| 出品公司: | EMD Biosciences(Novagen) |
|---|---|
| 別名: | pET23c, pet 23c |
| 質粒類型: | 大腸桿菌表達 |
| 表達水平: | 高 |
| 克隆方法: | 多克隆位點，限制性內切酶 |
| 載體大小: | 3664bp |
| 5' 測序引物: | T7 |
| 5' 測序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 載體標簽: | N-T7, C-His |
| 載體抗性: | Ampicillin |
| 克隆菌株: | DH5a |
| 表達菌株: | BL21系列 |
| 備注: |
Same as pET21abcd(+) but no lac; a,b,c,d vary by MCS.
|
| 產品目錄號: | 69747-3 |
| 穩定性: | 穩表達 |
| 組成型: | 組成型 |
| 病毒/非病毒: | 非病毒 |
| 表達宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表達手冊： |
pET表達手冊英文版 pET表達手冊中文版 |
| pET載體匯總: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
買家導航
pET-23c(+)載體 pET23c質粒基本信息
pET-23c(+)質粒圖譜 pET23c載體圖譜和pET-23c(+)載體序列 pET23c質粒序列多克隆位點信息
The maps for pET-23b(+), pET-23c(+) and pET-23d(+) are the same as pET-23a(+) (shown) with the following exceptions: pET-23b(+) is a 3665bp plasmid; subtract 1bp from each site beyond BamH I at 198. pET-23c(+) is a 3664bp plasmid; subtract 2bp from each site beyond BamH I at 198. pET-23d(+) is a 3663bp plasmid; the BamH I site is in the same reading frame as in pET-23c(+). An Nco I site is substituted for the Nde I site with a net 1bp deletion at position 238 of pET-23c(+). As a result, Nco I cuts pET-23d(+) at 234, and Nhe I cuts at 229. For the rest of the sites, subtract 3bp from each site beyond position 239 in pET-23a(+). Nde I does not cut pET-23d(+). Note also that Sty I is not unique in pET-23d(+).
pET-23c(+)質粒 pET23c載體簡介
pET-23a-d系列載體帶有N-T7標簽和C-His標簽。pET-23a-d系列載體與pET-21a-d系列載體相比，啟動子由pet21a系列載體的T7lac啟動子，更改為pET-23a-d載體的T7啟動子。獨特的酶切位點在載體圖譜中被標注出來。需要注意的是，這些載體序列是依據載體pBR322的方向來進行編號的，所以T7標簽表達區域在載體圖譜上是反向的。
pET-23c(+)質粒序列 pET23c載體序列
LOCUS pET-23c(+) 3664 bp DNA circular 11-SEP-2008
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
//gt12365.cn/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|gt12365.cn|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
promoter complement(301..317)
/vntifkey="30"
/label=T7\promoter
protein_bind complement(205..237)
/vntifkey="31"
/label=T7\tag
terminator complement(26..72)
/vntifkey="43"
/label=T7\terminator
protein_bind complement(140..157)
/vntifkey="31"
/label=His\tag
rep_origin complement(1448..1448)
/vntifkey="33"
/label=ColE1\origin
CDS complement(2209..3066)
/vntifkey="4"
/label=bla(amp)\sequence
rep_origin complement(3198..3653)
/vntifkey="33"
/label=f1\origin
BASE COUNT 919 a 935 c 925 g 885 t
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg
241 tatatctcct tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt gtggtctccc
301 tatagtgagt cgtattaatt tcgcgggatc gagatctcgg gcagcgttgg gtcctggcca
361 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
421 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
481 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
541 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
601 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
661 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
721 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
781 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
841 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
901 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
961 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
1021 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
1081 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
1141 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
1201 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
1261 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
1321 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
1381 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
1441 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
1501 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
1561 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
1621 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
1681 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
1741 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
1801 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
1861 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
1921 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
1981 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
2041 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
2101 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
2161 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
2221 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
2281 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
2341 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
2401 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
2461 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
2521 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
2581 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
2641 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
2701 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
2761 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
2821 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
2881 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
2941 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
3001 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
3061 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
3121 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
3181 ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
3241 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
3301 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
3361 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
3421 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
3481 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
3541 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
3601 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
3661 gcca
//
pET-23c(+)載體圖譜 pET23c質粒圖譜pdf版和pET-23c(+)載體序列 pET23c質粒序列等相關資料下載
pET-23c(+)質粒 pET23c載體應用舉例
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