出品公司: | EMD Biosciences (Novagen) |
---|---|
別名: | pET30c, pet 30c |
質粒類型: | 大腸桿菌蛋白表達 |
表達水平: | 高 |
克隆方法: | 多克隆位點,限制性內切酶 |
載體大小: | 5423 bp |
5' 測序引物: | T7 |
5' 測序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
載體標簽: | N-His, N-S, N-Thrombin, N-EK, C-His |
載體抗性: | Kanamycin |
備注: | N term thrombin cleavage site; N term enterokinase cleavage site; a,b,c vary by MCS |
產品目錄號: | 69911-3 |
穩定性: | 瞬時表達 Transient |
組成型: | 組成型 Constitutive |
病毒/非病毒: | 非病毒 |
表達宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3)、C43(DE3) |
pET表達手冊: |
pET表達手冊英文版 pET表達手冊中文版 |
pET載體匯總: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
買家導航
pET-30c(+)載體 pET30c質粒基本信息
pET-30c(+)質粒圖譜 pET30c載體圖譜和pET-30c(+)載體序列 pET30c質粒序列多克隆位點信息
Feature Name | Start | End | |
---|---|---|---|
T7_terminator | 129 | 1 | |
T7_Terminal_primer | 69 | 87 | |
EK | 231 | 220 | |
lacI | 303 | 1909 | |
T7_transl_en_RBS | 373 | 357 | |
lacO | 418 | 391 | |
T7_promoter | 436 | 418 | |
tet (300 - 563) | 472 | 735 | |
pBRrevBam_primer | 543 | 524 | |
tet (576 - 851) | 1968 | 2243 | |
ROP | 2718 | 2909 | |
pGEX_3_primer | 2925 | 2903 | |
pBR322_origin | 3943 | 3324 | |
KanR2 | 4049 | 4864 |
ORF | Start | End | |
---|---|---|---|
ORF frame 1 | 328 | 1077 | |
ORF frame 2 | 950 | 1909 | |
ORF frame 2 | 4049 | 4864 |
Enzyme Name | Cut |
---|---|
XhoI | 158 |
NotI | 166 |
EagI | 166 |
HindIII | 173 |
SalI | 179 |
SacI | 190 |
EcoRI | 192 |
BamHI | 198 |
EcoRV | 209 |
NcoI | 213 |
KpnI | 239 |
BglII | 242 |
BstBI | 269 |
NdeI | 347 |
XbaI | 385 |
BclI | 1191 |
ApaI | 1388 |
HpaI | 1683 |
FspI | 2259 |
NruI | 4137 |
SmaI | 4354 |
XmaI | 4352 |
pET-30c(+)質粒 pET30c載體簡介
pET-30c(+)質粒序列 pET30c載體序列
LOCUS pET-30c (+) 5423 bp DNA Circular SYN DEFINITION pET-30c (+) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI //gt12365.cn/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|gt12365.cn| COMMENT VNTOAUTHORAD1|Novagen Inc.| FEATURES Location/Qualifiers source 1..5423 /organism="pET-30 c (+)" /mol_type="other DNA" terminator complement(1..129) /label="T7_terminator" misc_feature 69..87 /label="T7_Terminal_primer" misc_feature complement(220..231) /label="EK" misc_feature 303..1909 /label="lacI" CDS 328..1077 /label="ORF frame 1" /translation="MMMMMVHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSI SILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLL GAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRE SIQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPR GTTTGGQTVVADWRCHLQSGPARAVANCRGD*" misc_feature complement(357..373) /label="T7_transl_en_RBS" /translation="MMMMMVHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSI SILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLL GAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRE SIQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPR GTTTGGQTVVADWRCHLQSGPARAVANCRGD*" misc_feature complement(391..418) /label="lacO" /translation="MMMMMVHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSI SILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLL GAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRE SIQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPR GTTTGGQTVVADWRCHLQSGPARAVANCRGD*" promoter complement(418..436) /label="T7_promoter" /translation="MMMMMVHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSI SILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLL GAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRE SIQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPR GTTTGGQTVVADWRCHLQSGPARAVANCRGD*" gene 472..735 /label="tet (300 - 563)" /gene="tet (300 - 563)" /translation="MMMMMVHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSI SILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLL GAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRE SIQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPR GTTTGGQTVVADWRCHLQSGPARAVANCRGD*" misc_feature complement(524..543) /label="pBRrevBam_primer" /translation="MMMMMVHMYISFLKLNKIISRGELLSAHNSPIVSRINFAGSRSI SILYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLL GAISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRE SIQGGECETSNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPR GTTTGGQTVVADWRCHLQSGPARAVANCRGD*" CDS 950..1909 /label="ORF frame 2" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" gene 1968..2243 /label="tet (576 - 851)" /gene="tet (576 - 851)" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" misc_feature 2718..2909 /label="ROP" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" misc_feature complement(2903..2925) /label="pGEX_3_primer" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" rep_origin complement(3324..3943) /label="pBR322_origin" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" gene 4049..4864 /label="KanR2" /gene="KanR2" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" CDS 4049..4864 /label="ORF frame 2" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*" ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccacagata tcccatggcc ttgtcgtcgt cgtcggtacc 241 cagatctggg ctgtccatgt gctggcgttc gaatttagca gcagcggttt ctttcatacc 301 agaaccgcgt ggcaccagac cagaagaatg atgatgatga tggtgcatat gtatatctcc 361 ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct 421 atagtgagtc gtattaattt cgcgggatcg agatcgatct cgatcctcta cgccggacgc 481 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc 541 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt 601 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc 661 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag 721 tcgcataagg gagagcgtcg agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg 781 tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac 841 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa 901 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct 961 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg 1021 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc 1081 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga 1141 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa 1201 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc 1261 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga 1321 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt 1381 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct 1441 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg 1501 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc 1561 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg 1621 tgcggacatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc 1681 gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct 1741 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa 1801 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 1861 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 1921 ttaatgtaag ttagctcact cattaggcac cgggatctcg accgatgccc ttgagagcct 1981 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga 2041 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg 2101 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa 2161 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga 2221 agcaggccat tatcgccggc atggcggccc cacgggtgcg catgatcgtg ctcctgtcgt 2281 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac 2341 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa 2401 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca 2461 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg 2521 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata 2581 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc 2641 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc 2701 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta 2761 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg 2821 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc 2881 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 2941 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 3001 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa 3061 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg 3121 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 3181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 3241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 3301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 3361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 3421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 3481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 3541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 3601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 3661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 3721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 3781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 3841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 3901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 3961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgaacaataa aactgtctgc 4021 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc 4081 taggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 4141 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga 4201 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 4261 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 4321 tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga 4381 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 4441 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 4501 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 4561 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga 4621 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 4681 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 4741 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 4801 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt 4861 ctaagaatta attcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 4921 gggttccgcg cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt 4981 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 5041 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 5101 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 5161 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 5221 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 5281 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 5341 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 5401 tacagggcgc gtcccattcg cca //
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pET-30c(+)質粒 pET30c載體應用舉例
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