LOCUS       pET-3b(+)    4639 bp     DNA  circular   SYN
DEFINITION  pET-3b
COMMENT     This file is created by Vector NTI
            //gt12365.cn/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|gt12365.cn|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
 FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="pET-3b"
                     /mol_type="other DNA"
     gene            86..1552
                     /label="tet (w/ gaps)"
                     /gene="tet (w/ gaps)"
     terminator      complement(379..507)
                     /label="T7_terminator"
     misc_feature    447..465
                     /label="T7_Terminal_primer"
     misc_feature    complement(518..550)
                     /label="T7_leader"
     misc_feature    complement(533..551)
                     /label="Xpress_fwd_primer"
     misc_feature    complement(559..575)
                     /label="T7_transl_en_RBS"
     promoter        complement(612..630)
                     /label="T7_promoter"
     misc_feature    complement(714..733)
                     /label="pBRrevBam_primer"
     CDS             767..1552
                     /label="ORF frame 2"
     CDS             complement(899..1357)
                     /label="ORF frame 1"
     misc_feature    2191..2382
                     /label="ROP"
     misc_feature    complement(2376..2398)
                     /label="pGEX_3_primer"
     gene            complement(3571..4431)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(3571..4431)
                     /label="ORF frame 2"
     promoter        complement(4473..4501)
                     /label="AmpR_promoter"
ORIGIN
    1 TTCTCATGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA
   61 TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG
  121 CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC CGGGCCTCTT
  181 GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT GGCGTGCTGC TAGCGCTATA
  241 TGCGTTGATG CAATTTCTAT GCGCACCCGT TCTCGGAGCA CTGTCCGACC GCTTTGGCCG
  301 CCGCCCAGTC CTGCTCGCTT CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC
  361 CACACCCGTC CTGTGGATAT CCGGATATAG TTCCTCCTTT CAGCAAAAAA CCCCTCAAGA
  421 CCCGTTTAGA GGCCCCAAGG GGTTATGCTA GTTATTGCTC AGCGGTGGCA GCAGCCAACT
  481 CAGCTTCCTT TCGGGCTTTG TTAGCAGCCG GATCCCGACC CATTTGCTGT CCACCAGTCA
  541 TGCTAGCCAT ATGTATATCT CCTTCTTAAA GTTAAACAAA ATTATTTCTA GAGGGAAACC
  601 GTTGTGGTCT CCCTATAGTG AGTCGTATTA ATTTCGCGGG ATCGAGATCT CGATCCTCTA
  661 CGCCGGACGC ATCGTGGCCG GCATCACCGG CGCCACAGGT GCGGTTGCTG GCGCCTATAT
  721 CGCCGACATC ACCGATGGGG AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT
  781 CGGCGTGGGT ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCA
  841 TGCACCATTC CTTGCGGCGG CGGTGCTCAA CGGCCTCAAC CTACTACTGG GCTGCTTCCT
  901 AATGCAGGAG TCGCATAAGG GAGAGCGTCG ACCGATGCCC TTGAGAGCCT TCAACCCAGT
  961 CAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT
 1021 TATCATGCAA CTCGTAGGAC AGGTGCCGGC AGCGCTCTGG GTCATTTTCG GCGAGGACCG
 1081 CTTTCGCTGG AGCGCGACGA TGATCGGCCT GTCGCTTGCG GTATTCGGAA TCTTGCACGC
 1141 CCTCGCTCAA GCCTTCGTCA CTGGTCCCGC CACCAAACGT TTCGGCGAGA AGCAGGCCAT
 1201 TATCGCCGGC ATGGCGGCCG ACGCGCTGGG CTACGTCTTG CTGGCGTTCG CGACGCGAGG
 1261 CTGGATGGCC TTCCCCATTA TGATTCTTCT CGCTTCCGGC GGCATCGGGA TGCCCGCGTT
 1321 GCAGGCCATG CTGTCCAGGC AGGTAGATGA CGACCATCAG GGACAGCTTC AAGGATCGCT
 1381 CGCGGCTCTT ACCAGCCTAA CTTCGATCAC TGGACCGCTG ATCGTCACGG CGATTTATGC
 1441 CGCCTCGGCG AGCACATGGA ACGGGTTGGC ATGGATTGTA GGCGCCGCCC TATACCTTGT
 1501 CTGCCTCCCC GCGTTGCGTC GCGGTGCATG GAGCCGGGCC ACCTCGACCT GAATGGAAGC
 1561 CGGCGGCACC TCGCTAACGG ATTCACCACT CCAAGAATTG GAGCCAATCA ATTCTTGCGG
 1621 AGAACTGTGA ATGCGCAAAC CAACCCTTGG CAGAACATAT CCATCGCGTC CGCCATCTCC
 1681 AGCAGCCGCA CGCGGCGCAT CTCGGGCAGC GTTGGGTCCT GGCCACGGGT GCGCATGATC
 1741 GTGCTCCTGT CGTTGAGGAC CCGGCTAGGC TGGCGGGGTT GCCTTACTGG TTAGCAGAAT
 1801 GAATCACCGA TACGCGAGCG AACGTGAAGC GACTGCTGCT GCAAAACGTC TGCGACCTGA
 1861 GCAACAACAT GAATGGTCTT CGGTTTCCGT GTTTCGTAAA GTCTGGAAAC GCGGAAGTCA
 1921 GCGCCCTGCA CCATTATGTT CCGGATCTGC ATCGCAGGAT GCTGCTGGCT ACCCTGTGGA
 1981 ACACCTACAT CTGTATTAAC GAAGCGCTGG CATTGACCCT GAGTGATTTT TCTCTGGTCC
 2041 CGCCGCATCC ATACCGCCAG TTGTTTACCC TCACAACGTT CCAGTAACCG GGCATGTTCA
 2101 TCATCAGTAA CCCGTATCGT GAGCATCCTC TCTCGTTTCA TCGGTATCAT TACCCCCATG
 2161 AACAGAAATC CCCCTTACAC GGAGGCATCA GTGACCAAAC AGGAAAAAAC CGCCCTTAAC
 2221 ATGGCCCGCT TTATCAGAAG CCAGACATTA ACGCTTCTGG AGAAACTCAA CGAGCTGGAC
 2281 GCGGATGAAC AGGCAGACAT CTGTGAATCG CTTCACGACC ACGCTGATGA GCTTTACCGC
 2341 AGCTGCCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG
 2401 ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA
 2461 GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA TAGCGGAGTG
 2521 TATACTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATATGCG
 2581 GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT CTTCCGCTTC
 2641 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC
 2701 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC
 2761 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG
 2821 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
 2881 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT
 2941 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT
 3001 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG
 3061 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT
 3121 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
 3181 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG
 3241 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA
 3301 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT
 3361 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC
 3421 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
 3481 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA
 3541 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT
 3601 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC
 3661 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG
 3721 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
 3781 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT
 3841 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTGCAG GCATCGTGGT
 3901 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT
 3961 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT
 4021 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT
 4081 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT
 4141 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAACAC GGGATAATAC
 4201 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA
 4261 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA
 4321 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA
 4381 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT
 4441 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA
 4501 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC
 4561 TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG
 4621 GCCCTTTCGT CTTCAAGAA
//