| 出品公司: | EMD Biosciences (Novagen) |
|---|---|
| 別名: | pET41 EK/LIC, pET 41 EK/LIC |
| 質粒類型: | 大腸桿菌蛋白表達 |
| 表達水平: | 高 |
| 克隆方法: | 多克隆位點，限制性內切酶 |
| 載體大小: | 5947 bp |
| 5' 測序引物: | T7或pGEX-5‘ |
| 5' 測序引物序列: | T7: 5'-TAATACGACTCACTATAGGG-3'; pGEX-5': 5'-GGGCTGGCAAGCCACGTTTGGTG-3' |
| 載體標簽: | N-GST, N-His, N-EK, C-His |
| 載體抗性: | Kanamycin |
| 備注: |
For directional cloning of PCR-amplified DNA; ligation independent cloning; enterokinase cleavage site.
|
| 產品目錄號: | 71071-3 |
| 穩定性: | 瞬時表達 Transient |
| 組成型: | 組成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表達宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表達手冊： |
pET表達手冊英文版 pET表達手冊中文版 |
| pET載體匯總: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
買家導航
pET-41 EK/LIC載體 pET41 EK/LIC質粒基本信息
pET-41 EK/LIC質粒圖譜 pET41 EK/LIC載體圖譜和pET-41 EK/LIC載體序列 pET41 EK/LIC質粒序列多克隆位點信息
pET-41 EK/LIC質粒 pET41 EK/LIC載體簡介
pET-41 EK/LIC載體是用來快速定向PCR高通量質粒構建的載體。本載體帶有N-GST標簽和His標簽，S標簽。獨特的酶切位點在載體圖譜中被標注出來。需要注意的是，這些載體序列是依據載體pBR322的方向來進行編號的，所以T7標簽表達區域在載體圖譜上是反向的。
T7 RNA聚合酶啟動的克隆和表達區域在質粒圖譜中也被標注了出來。質粒的F1復制子是被定向的，所以在T7噬菌體聚合酶的作用下，包含有蛋白編碼序列的病毒 粒子能夠產生，并啟動蛋白表達，同時蛋白表達將被T7終止子序列(Cat. No. 69337-3)的作用下終止蛋白翻譯。
pET-41a載體上面含有一個EK蛋白酶切位點，當將目的基因使用載體上面的PshAI限制性內切酶位點插入進去時，可以通過EK蛋白酶將載體上的融合的所有氨基酸序列包括GST標簽序列，全部切除掉。
pET-41 EK/LIC質粒序列 pET41 EK/LIC載體序列
LOCUS pET-41 Ek/LIC 5947 bp DNA Circular SYN
DEFINITION pET-41 Ek/LIC
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
//gt12365.cn/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|gt12365.cn|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..5947
/organism="pET-41 Ek/LIC"
/mol_type="other DNA"
terminator complement(1..120)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
CDS complement(147..1109)
/label="ORF frame 3"
misc_feature complement(279..293)
/label="EK"
misc_feature complement(324..368)
/label="S15"
misc_feature complement(411..428)
/label="6xHIS"
gene complement(459..1109)
/label="GST (variant)"
/gene="GST (variant)"
CDS 1110..1838
/label="ORF frame 3"
misc_feature complement(1118..1134)
/label="T7_transl_en_RBS"
misc_feature complement(1152..1179)
/label="lacO"
promoter complement(1179..1197)
/label="T7_promoter"
gene 1233..1496
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(1285..1304)
/label="pBRrevBam_primer"
misc_feature 1579..2670
/label="lacI"
CDS 1711..2670
/label="ORF frame 1"
misc_feature 3242..3433
/label="ROP"
misc_feature complement(3427..3449)
/label="pGEX_3_primer"
rep_origin complement(3848..4467)
/label="pBR322_origin"
gene 4573..5388
/label="KanR2"
/gene="KanR2"
CDS 4573..5388
/label="ORF frame 1"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgtttagcag cctaggtatt aatcaattag tggtggtggt ggtggtggtg gtgctcgagt
181 gcggccgcaa gcttgtcgac ggagctcgcc tgcaggcgcg ccaaggcctg tacagaattc
241 ggatccccga tatccatggt tgaggagaag cccgggctct tgtcgtcgtc atcaccagaa
301 ccaccaccgg tacccagatc tgggctgtcc atgtgctggc gttcgaattt agcagcagcg
361 gtttctttca taccaattgc agtactaccg cgtggcacca gacccgcgga gtgatggtga
421 tggtgatgac cagaaccact agttgaacca tccgattttg gaggatggtc gccaccacca
481 aacgtggctt gccagccctg caaaggccat gctatatact tgctggattt caagtactta
541 tcaatttgtg ggatagcttc aatacgtttt ttaaaacaaa ctaattttgg gaacgcatcc
601 aggcacattg ggtccatgta taaaacaaca tcaagagcgt catacaacat gaagtcagga
661 tgggttacat gatcaccatt taaatatgtt ttatgacata aacgatcttc gaacattttc
721 agcatttcag gtagcttgct aagaaaatca actttgagag tttcaaagtc tttactatat
781 gcaattctcg aaacaccgta tctaatatcc aaaaccgctc cttcaagcat tgaaatctct
841 gcacgctctt ttggacaacc acccaacatg ttgtgcttgt cagctatata acgtatgatg
901 gccatagact gtgttaattt aacatcacca tcaatataat aaggaagatt gggaaactcc
961 aaacccaatt caaacttttt gtttcgccat ttatcacctt catcgcgctc atacaaatgc
1021 tcttcatatt tttcttcaag atattccaaa agaagtcgag tgggttgcac aaggccctta
1081 attttccaat aacctagtat aggggacata tgtatatctc cttcttaaag ttaaacaaaa
1141 ttatttctag aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt
1201 tcgcgggatc gagatcgatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg
1261 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
1321 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
1381 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
1441 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
1501 gagatcccgg acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg
1561 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
1621 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
1681 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
1741 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
1801 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
1861 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
1921 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
1981 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
2041 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
2101 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
2161 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
2221 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
2281 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
2341 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggacat ctcggtagtg
2401 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
2461 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
2521 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
2581 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
2641 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcac
2701 tcattaggca ccgggatctc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
2761 ccggtgggcg cggggcatga ctagcatgat cgtgctcctg tcgttgagga cccggctagg
2821 ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag
2881 cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg
2941 tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg
3001 catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg
3061 gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc
3121 ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct
3181 ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc
3241 agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt
3301 aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc
3361 gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg
3421 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
3481 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
3541 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
3601 cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga
3661 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
3721 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
3781 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
3841 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
3901 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
3961 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
4021 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
4081 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
4141 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
4201 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
4261 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
4321 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
4381 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
4441 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
4501 actcacgtta agggattttg gtcatgaaca ataaaactgt ctgcttacat aaacagtaat
4561 acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctctaggcc gcgattaaat
4621 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
4681 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
4741 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
4801 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
4861 ctcaccactg cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca
4921 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
4981 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
5041 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
5101 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
5161 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
5221 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
5281 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
5341 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaaga attaattcat
5401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
5461 tccccgaaaa gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa
5521 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
5581 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
5641 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
5701 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
5761 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
5821 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
5881 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
5941 ttcgcca
//
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pET-41 EK/LIC質粒 pET41 EK/LIC載體應用舉例
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