| 出品公司: | ATCC |
|---|---|
| 載體名稱: | YEplac112 |
| 質粒類型: | 釀酒酵母表達載體；2μ質粒型載體；酵母附加體型載體 |
| 高拷貝/低拷貝: | 高拷貝 |
| 克隆方法: | 限制性內切酶，多克隆位點 |
| 啟動子: | lacZ |
| 載體大小: | 4989 bp |
| 5' 測序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
| 3' 測序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
| 載體標簽: | -- |
| 載體抗性: | 氨芐青霉素 |
| 篩選標記: | TRP1 |
| 表達菌株: | 釀酒酵母、白色念珠菌 |
| 備注: | -- |
| 產品目錄號: | 87590 |
| 穩定性: | 穩表達 |
| 組成型/誘導型: | 誘導型 |
| 病毒/非病毒: | 非病毒 |
買家導航
YEplac112載體質粒基本信息
YEplac112質粒圖譜載體圖譜和YEplac112載體序列質粒序列多克隆位點信息
YEplac112質粒載體簡介
YEplac112質粒序列載體序列
LOCUS YEplac112 4989 bp DNA SYN
DEFINITION YEplac112
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4989
/organism="YEplac112"
/mol_type="other DNA"
promoter 142..171
/label="lac_promoter"
misc_feature 185..207
/label="M13_pUC_rev_primer"
promoter 206..224
/label="M13_reverse_primer"
misc_feature 287..442
/label="lacZ_a"
promoter complement(290..306)
/label="M13_forward20_primer"
misc_feature complement(299..321)
/label="M13_pUC_fwd_primer"
rep_origin complement(743..1907)
/label="2micron2_origin"
gene complement(2201..2875)
/label="TRP1"
/gene="TRP1"
CDS complement(2201..2875)
/label="ORF frame 3"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK*"
promoter 3118..3146
/label="AmpR_promoter"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK*"
gene 3188..4048
/label="Ampicillin"
/gene="Ampicillin"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK*"
CDS 3188..4048
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 4203..4822
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tatcggatcg tacttgttac ccatcattga attttgaaca
541 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat
601 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc
661 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc
721 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg
781 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
841 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
901 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
961 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
1021 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
1081 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
1141 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
1201 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
1261 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
1321 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
1381 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
1441 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
1501 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
1561 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
1621 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag
1681 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac
1741 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag
1801 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat
1861 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc
1921 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg
1981 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa
2041 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat
2101 aagctgtcaa acatgagaat tgatctttta tgcttgcttt tcaaaaggct tgcaggcaag
2161 tgcacaaaca atacttaaat aaatactact cagtaataac ctatttctta gcatttttga
2221 cgaaatttgc tattttgtta gagtctttta caccatttgt ctccacacct ccgcttacat
2281 caacaccaat aacgccattt aatctaagcg catcaccaac attttctggc gtcagtccac
2341 cagctaacat aaaatgtaag ctctcggggc tctcttgcct tccaacccag tcagaaatcg
2401 agttccaatc caaaagttca cctgtcccac ctgcttctga atcaaacaag ggaataaacg
2461 aatgaggttt ctgtgaagct gcactgagta gtatgttgca gtcttttgga aatacgagtc
2521 ttttaataac tggcaaaccg aggaactctt ggtattcttg ccacgactca tctccatgca
2581 gttggacgat atcaatgccg taatcattga ccagagccaa aacatcctcc ttaggttgat
2641 tacgaaacac gccaaccaag tatttcggag tgcctgaact atttttatat gcttttacaa
2701 gacttgaaat tttccttgca ataaccgggt caattgttct ctttctattg ggcacacata
2761 taatacccag caagtcagca tcggaatcta gtgcacattc tgcggcctct gtgctctgca
2821 agccgcaaac tttcaccaat ggaccagaac tacctgtgaa attaataaca gacatactcc
2881 aagctgcctt tgtgtgctta atcacgtata ctcacgtgct caatagtcac caatgccctc
2941 cctcttggcc ctctcctttt cttttttcga ccgaattaat tcttgaagac gaaagggcct
3001 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
3061 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
3121 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
3181 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
3241 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
3301 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
3361 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
3421 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
3481 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
3541 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
3601 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
3661 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
3721 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
3781 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
3841 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
3901 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
3961 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
4021 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
4081 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
4141 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
4201 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
4261 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
4321 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
4381 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
4441 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
4501 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
4561 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
4621 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
4681 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
4741 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
4801 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
4861 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
4921 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
4981 agcggaaga
//