| 出品公司: | Euroscarf |
|---|---|
| 載體名稱: | pFA6a-natMX6 |
| 質粒類型: | 酵母表達載體，酵母基因編輯質粒 |
| 高拷貝/低拷貝: | 高拷貝 |
| 克隆方法: | 限制性內切酶，多克隆位點 |
| 啟動子: | AgTEF |
| 載體大小: | 3.7 kb |
| 5’測序引物: | -- |
| 3’測序引物: | -- |
| 載體標簽: | -- |
| 載體抗性: | 氨芐青霉素 |
| 克隆菌株: | DH5 alpha; 等 |
| 宿主細胞（系）: | 酵母菌 |
| 產品目錄號: | P30437 |
| 穩定性: | 瞬表達 |
| 組成型/誘導型: | 組成型 |
| 病毒/非病毒: | 非病毒 |
| 備注: | pFA6a-natMX6是酵母菌基因編輯載體。 |
| 參考文獻： | Hentges P, Van Driessche B, Tafforeau L, Vandenhaute J , Carr AM . Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe. Yeast. 2005; 22, 1013-9. |
買家導航
pFA6a-natMX6載體質粒基本信息
pFA6a-natMX6質粒圖譜載體圖譜和pFA6a-natMX6載體序列質粒序列多克隆位點信息
pFA6a-natMX6質粒載體簡介
pFA6a-natMX6質粒序列載體序列
LOCUS pFA6a-natMX6 3704 bp DNA circular SYN 07-MAR-2024
DEFINITION Plasmid for yeast gene deletion using the natMX6 selectable marker
conferring nourseothricin resistance.
ACCESSION .
VERSION .
KEYWORDS pFA6a-natMX6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3704)
AUTHORS Hentges P, Van Driessche B, Tafforeau L, Vandenhaute J, Carr AM.
TITLE Three novel antibiotic marker cassettes for gene disruption and
marker switching in Schizosaccharomyces pombe.
JOURNAL Yeast 2005;22:1013-9.
PUBMED 16200533
REFERENCE 2 (bases 1 to 3704)
AUTHORS EUROSCARF
TITLE Direct Submission
JOURNAL Exported Jul 14, 2024 from SnapGene 6.0.2
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Yeast";
date: "2005"; volume: "22"; pages: "1013-9"
FEATURES Location/Qualifiers
source 1..3704
/lab_host="Schizosaccharomyces pombe"
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 40..59
/label=F1
/note="Forward primer for gene deletion. This primer
includes a BamHI recognition sequence. A gene-specific
sequence should be added at the 5' end of the primer."
/note="color: black; sequence: CGGATCCCCGGGTTAATTAA"
gene 113..1235
/label=natMX6
/note="yeast selectable marker conferring nourseothricin
resistance"
/note="color: #ff7f50; direction: RIGHT"
promoter 113..456
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 457..1032
/codon_start=1
/gene="Streptomyces noursei nat1"
/product="nourseothricin acetyltransferase"
/label=NrsR
/note="confers resistance to nourseothricin"
/note="color: #ccffcc"
/translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVISYSAWNR
RLTVEDIEVAPEHRGHGVGRALMGLATEFAGERGAGHLWLEVTNVNAPAIHAYRRMGFT
LCGLDTALYDGTASDGERQALYMSMPCP"
terminator 1038..1235
/label=TEF terminator
/note="Ashbya gossypii TEF terminator"
/note="color: #ffffff"
primer_bind complement(1266..1285)
/label=R1
/note="Reverse primer for gene deletion or C-terminal
tagging. This primer includes an EcoRI recognition
sequence. A gene-specific sequence should be added at the
5' end of the primer."
/note="color: black; sequence: GAATTCGAGCTCGTTTAAAC"
promoter complement(1340..1358)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
/note="color: #ffffff; direction: LEFT"
rep_origin complement(1616..2204)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS complement(2375..3235)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
1: 2375 .. 3166 / #ccffcc
2: 3167 .. 3235 / #ccffcc / signal sequence
Cleavage site after base 3166"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3236..3340)
/gene="bla"
/label=AmpR promoter
/note="color: #ffffff; direction: LEFT"
promoter 3686..3704
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
/note="color: #ffffff; direction: RIGHT"
ORIGIN
1 acgcggccgc cagctgaagc ttcgtacgct gcaggtcgac ggatccccgg gttaattaag
61 gcgcgccaga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga
121 ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg
181 tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca tacattttga
241 tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa
301 cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa
361 aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg
421 atacagttct cacatcacat ccgaacataa acaaccatgg gtaccactct tgacgacacg
481 gcttaccggt accgcaccag tgtcccgggg gacgccgagg ccatcgaggc actggatggg
541 tccttcacca ccgacaccgt cttccgcgtc accgccaccg gggacggctt caccctgcgg
601 gaggtgccgg tggacccgcc cctgaccaag gtgttccccg acgacgaatc ggacgacgaa
661 tcggacgacg gggaggacgg cgacccggac tcccggacgt tcgtcgcgta cggggacgac
721 ggcgacctgg cgggcttcgt ggtcatctcg tactcggcgt ggaaccgccg gctgaccgtc
781 gaggacatcg aggtcgcccc ggagcaccgg gggcacgggg tcgggcgcgc gttgatgggg
841 ctcgcgacgg agttcgccgg cgagcggggc gccgggcacc tctggctgga ggtcaccaac
901 gtcaacgcac cggcgatcca cgcgtaccgg cggatggggt tcaccctctg cggcctggac
961 accgccctgt acgacggcac cgcctcggac ggcgagcggc aggcgctcta catgagcatg
1021 ccctgcccct aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg
1081 tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt
1141 tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat
1201 gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc
1261 atccagttta aacgagctcg aattcatcga tgatatcaga tccactagtg gcctatgcgg
1321 ccgcggatct gccggtctcc ctatagtgag tcgtattaat ttcgataagc caggttaacc
1381 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
1441 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
1501 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
1561 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
1621 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
1681 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
1741 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
1801 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
1861 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
1921 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
1981 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
2041 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
2101 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
2161 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
2221 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
2281 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
2341 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
2401 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
2461 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
2521 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
2581 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
2641 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
2701 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
2761 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
2821 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
2881 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
2941 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
3001 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
3061 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
3121 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
3181 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
3241 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
3301 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
3361 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
3421 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
3481 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
3541 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
3601 ttgtactgag agtgcaccat atggacatat tgtcgttaga acgcggctac aattaataca
3661 taaccttatg tatcatacac atacgattta ggtgacacta taga
//