| 出品公司: | Invitrogen |
|---|---|
| 載體名稱: | pPICZC, pPICZ C |
| 質粒類型: | 畢赤酵母蛋白表達載體 |
| 表達水平: | 高拷貝 |
| 啟動子: | AOX1 |
| 克隆方法: | 多克隆位點，限制性內切酶 |
| 載體大小: | 3329 bp |
| 5' 測序引物及序列: | 5´ AOX1:5´-GACTGGTTCCAATTGACAAGC-3´ |
| 3' 測序引物及序列: | 3´ AOX1:5´-GCAAATGGCATTCTGACATCC-3´ |
| 載體標簽: | C-Myc, C-His |
| 載體抗性: | Zeocin 博來霉素 |
| 篩選標記: | Zeocin 博來霉素 |
| 備注: |
插入基因是必需包含起始密碼子ATG。
|
| 產品目錄號: | V190-20 |
| 穩定性: | 穩定 Stable |
| 組成型: | 組成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
買家導航
pPICZ C載體 pPICZC質粒基本信息
pPICZ C質粒圖譜 pPICZC載體圖譜和pPICZ C載體序列 pPICZC質粒序列多克隆位點信息
pPICZ C質粒 pPICZC載體簡介
pPICZ A，B和C是3.3 kb的畢赤酵母蛋白載體。表達的重組蛋白是融合蛋白，含有一個C-端多肽，多太重含c-myc和C-His標簽。載體能夠在畢赤酵母中利用甲醇誘導的高水 平的表達目的蛋白，并且可以用在任何畢赤酵母中，包括X33，GS115菌株，SMD1168H，KM71H。
pPICZ系列載體包含以下元素：
•5'片段含有AOX1啟動子的嚴格調控，利用甲醇誘導表達任何感興趣的基因（Ellis等，1985; Koutz等人，1989;tschopp等人，1987A）。
•Zeocin抗性基因在大腸桿菌和畢赤酵母都能用于篩選（Baron等人,1992; Drocourt等人，1990）。
•C-端含Myc和His標簽，可以用于檢測和純化重組蛋白。
•pPICZ A, B, C三種讀碼框使得可以將基因克隆入載體而不發生任何移碼突變。
pPICZ C質粒序列 pPICZC載體序列
LOCUS pPICZ C 3329 bp ds-DNA circular SYN 04-二月-2013
DEFINITION Pichia pastoris vector for methanol-inducible intracellular
expression of a protein.
ACCESSION .
VERSION .
KEYWORDS pPICZ C
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
COMMENT This file is created by Vector NTI
//gt12365.cn/
COMMENT VNTAUTHORNAME|gt12365.cn|
COMMENT Linearize with SacI, PmeI, or BstXI for integration at the AOX1
locus.
FEATURES Location/Qualifiers
source 1..3329
/organism="synthetic DNA construct"
/lab_host="Pichia pastoris"
/mol_type="other DNA"
promoter 2..940
/gene="Pichia pastoris AOX1"
/note="AOX1 promoter"
/note="inducible promoter, regulated by methanol"
/note="color: #ffffff; direction: RIGHT"
misc_feature 932..1010
/note="MCS"
/note="multiple cloning site"
/note="color: #99ccff"
CDS 1011..1040
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/note="color: #cc99b2"
/translation="EQKLISEEDL"
CDS 1056..1073
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/note="color: #cc99b2"
/translation="HHHHHH"
terminator 1153..1399
/gene="Pichia pastoris AOX1"
/note="AOX1 terminator"
/note="transcription terminator for AOX1"
/note="color: #ffffff"
promoter 1439..1825
/gene="S. cerevisiae TEF1"
/note="TEF1 promoter"
/note="promoter for EF-1-alpha"
/note="color: #ffffff; direction: RIGHT"
promoter 1833..1880
/note="EM7 promoter"
/note="synthetic bacterial promoter?"
/note="color: #ffffff; direction: RIGHT"
CDS 1899..2273
/codon_start=1
/gene="Sh?ble from?Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="BleoR"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/note="color: #ccffcc"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
terminator 2339..2586
/gene="S. cerevisiae CYC1"
/note="CYC1 terminator"
/note="transcription terminator for CYC1"
/note="color: #ffffff"
rep_origin complement(2661..3249)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
ORIGIN
1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
901 caacttgaga agatcaaaaa acaactaatt attcgaaacg aggaattcac gtggcccagc
961 cggccgtctc ggatcggtac ctcgagccgc ggcggccgcc agcttacgta gaacaaaaac
1021 tcatctcaga agaggatctg aatagcgccg tcgaccatca tcatcatcat cattgagttt
1081 gtagccttag acatgactgt tcctcagttc aagttgggca cttacgagaa gaccggtctt
1141 gctagattct aatcaagagg atgtcagaat gccatttgcc tgagagatgc aggcttcatt
1201 tttgatactt ttttatttgt aacctatata gtataggatt ttttttgtca ttttgtttct
1261 tctcgtacga gcttgctcct gatcagccta tctcgcagct gatgaatatc ttgtggtagg
1321 ggtttgggaa aatcattcga gtttgatgtt tttcttggta tttcccactc ctcttcagag
1381 tacagaagat taagtgagac cttcgtttgt gcggatcccc cacacaccat agcttcaaaa
1441 tgtttctact ccttttttac tcttccagat tttctcggac tccgcgcatc gccgtaccac
1501 ttcaaaacac ccaagcacag catactaaat tttccctctt tcttcctcta gggtgtcgtt
1561 aattacccgt actaaaggtt tggaaaagaa aaaagagacc gcctcgtttc tttttcttcg
1621 tcgaaaaagg caataaaaat ttttatcacg tttctttttc ttgaaatttt tttttttagt
1681 ttttttctct ttcagtgacc tccattgata tttaagttaa taaacggtct tcaatttctc
1741 aagtttcagt ttcatttttc ttgttctatt acaacttttt ttacttcttg ttcattagaa
1801 agaaagcata gcaatctaat ctaaggggcg gtgttgacaa ttaatcatcg gcatagtata
1861 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
1921 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
1981 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
2041 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
2101 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
2161 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
2221 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtcc
2281 gacggcggcc cacgggtccc aggcctcgga gatccgtccc ccttttcctt tgtcgatatc
2341 atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa
2401 aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta
2461 gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac
2521 gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta
2581 atttgcaagc tggagaccaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
2641 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
2701 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
2761 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
2821 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
2881 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
2941 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
3001 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
3061 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
3121 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
3181 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
3241 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
3301 tcacgttaag ggattttggt catgagatc
//