| 出品公司: | Invitrogen |
|---|---|
| 載體名稱: | pYES2-NTC, pYES2/NT C |
| 質粒類型: | 釀酒酵母蛋白表達載體 |
| 表達水平: | 高拷貝 |
| 誘導方法: | 半乳糖 |
| 啟動子: | GAL1 |
| 克隆方法: | 多克隆位點，限制性內切酶 |
| 載體大小: | 6037 bp |
| 5' 測序引物及序列: | T7: TAATACGACTCACTATAGGG |
| 3' 測序引物及序列: | CYC1 Terminator: GTGACATAACTAATTACATGATG |
| 載體標簽: | N-His, N-Xpress, C-His, C-V5 |
| 載體抗性: | 氨芐 |
| 篩選標記: | URA3 |
| 備注: | 利用半乳糖誘導蛋白在釀酒酵母中表達。 |
| 產品目錄號: | V8252-20 |
| 穩定性: | 穩定 Stable |
| 組成型/誘導型: | 誘導型 |
| 病毒/非病毒: | 非病毒 |
買家導航
pYES2-NTC載體質粒基本信息
pYES2-NTC質粒圖譜載體圖譜和pYES2-NTC載體序列質粒序列多克隆位點信息
pYES2-NTC質粒載體簡介
pYES2 NTC的是一個6.0 kb的載體，設計用來在釀酒酵母（Saccharomyces cerevisiae）中誘導表達重組蛋白。
該載體包含以下元素：
1. 酵母GAL1啟動子，能夠在釀酒酵母中被半乳糖高水平的誘導蛋白表達目的蛋白，同時能夠被葡萄糖抑制表達
2.多克隆位點可以使用的很多限制酶切位點，便于基因插入。
3.CYC1終止子能夠有效終止mRNA的轉錄。
4.能夠利用URA3基因篩選帶有ura3基因型的酵母宿主菌株轉化子。
5.氨芐抗性基因能夠方便在大腸桿菌中的進行載體篩選。
6. 載體本身帶有N端His和Xpress標簽，同時載體C端也帶有V5抗原表位和His純化標簽。
8. 在酵母中，利用載體上的2μ復制子維持質粒的高拷貝。
pYES2-NTC質粒序列載體序列
LOCUS pYES2/NT C 6037 bp DNA circular SYN
DEFINITION pYES2/NT C
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
//gt12365.cn/
COMMENT VNTAUTHORNAME|gt12365.cn|
FEATURES Location/Qualifiers
source 1..6037
/organism="pYES2/NT C"
/mol_type="other DNA"
promoter 1..451
/label="GAL1_promoter"
misc_feature 414..437
/label="GAL1_primer"
promoter 475..493
/label="T7_promoter"
misc_feature 542..560
/label="Xpress_fwd_primer"
misc_feature 543..575
/label="T7_leader"
misc_feature 579..602
/label="Xpress_EK"
terminator 790..1035
/label="CYC1_terminator"
misc_feature complement(799..817)
/label="CYC1_primer"
misc_feature complement(799..817)
/label="pYESTrp_rev_primer"
rep_origin complement(1263..1882)
/label="pBR322_origin"
CDS complement(2037..2897)
/label="ORF frame 3"
CDS complement(2993..3796)
/label="ORF frame 1"
gene complement(2996..3796)
/label="URA3"
/gene="URA3"
CDS 3285..3761
/label="ORF frame 3"
promoter complement(3798..4023)
/label="URA3_promoter"
rep_origin 4026..5497
/label="2micron_origin"
ORIGIN
1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
481 GACTCACTAT AGGGAATATT AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG
541 GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA
601 AGGTACCCGG ATCCAGTGTG GTGGAATTCT GCAGATATCC AGCACAGTGG CGGCCGCTCG
661 AGTCTAGAGG GCCCTTCGAA GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
721 CGCGTACCGG TCATCATCAC CATCACCATT GAGTTTAAAC CCGCTGATCC TAGAGGGCCG
781 CATCATGTAA TTAGTTATGT CACGCTTACA TTCACGCCCT CCCCCCACAT CCGCTCTAAC
841 CGAAAAGGAA GGAGTTAGAC AACCTGAAGT CTAGGTCCCT ATTTATTTTT TTATAGTTAT
901 GTTAGTATTA AGAACGTTAT TTATATTTCA AATTTTTCTT TTTTTTCTGT ACAGACGCGT
961 GTACGCATGT AACATTATAC TGAAAACCTT GCTTGAGAAG GTTTTGGGAC GCTCGAAGGC
1021 TTTAATTTGC AAGCTGCGGC CCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT
1081 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG
1141 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
1201 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGCCCAGGAA CCGTAAAAAG
1261 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
1321 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
1381 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
1441 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG
1501 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
1561 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
1621 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
1681 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT
1741 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
1801 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
1861 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
1921 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
1981 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
2041 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT
2101 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGC GCTTACCATC TGGCCCCAGT
2161 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
2221 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
2281 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
2341 GTTGGCATTG CTACAGGCAT CGTGGTGTCA CTCTCGTCGT TTGGTATGGC TTCATTCAGC
2401 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
2461 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
2521 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
2581 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
2641 TGCCCGGCGT CAATACGGGA TAATAGTGTA TCACATAGCA GAACTTTAAA AGTGCTCATC
2701 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
2761 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
2821 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
2881 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATGGGTAA TAACTGATAT AATTAAATTG
2941 AAGCTCTAAT TTGTGAGTTT AGTATACATG CATTTACTTA TAATACAGTT TTTTAGTTTT
3001 GCTGGCCGCA TCTTCTCAAA TATGCTTCCC AGCCTGCTTT TCTGTAACGT TCACCCTCTA
3061 CCTTAGCATC CCTTCCCTTT GCAAATAGTC CTCTTCCAAC AATAATAATG TCAGATCCTG
3121 TAGAGACCAC ATCATCCACG GTTCTATACT GTTGACCCAA TGCGTCTCCC TTGTCATCTA
3181 AACCCACACC GGGTGTCATA ATCAACCAAT CGTAACCTTC ATCTCTTCCA CCCATGTCTC
3241 TTTGAGCAAT AAAGCCGATA ACAAAATCTT TGTCGCTCTT CGCAATGTCA ACAGTACCCT
3301 TAGTATATTC TCCAGTAGAT AGGGAGCCCT TGCATGACAA TTCTGCTAAC ATCAAAAGGC
3361 CTCTAGGTTC CTTTGTTACT TCTTCTGCCG CCTGCTTCAA ACCGCTAACA ATACCTGGGC
3421 CCACCACACC GTGTGCATTC GTAATGTCTG CCCATTCTGC TATTCTGTAT ACACCCGCAG
3481 AGTACTGCAA TTTGACTGTA TTACCAATGT CAGCAAATTT TCTGTCTTCG AAGAGTAAAA
3541 AATTGTACTT GGCGGATAAT GCCTTTAGCG GCTTAACTGT GCCCTCCATG GAAAAATCAG
3601 TCAAGATATC CACATGTGTT TTTAGTAAAC AAATTTTGGG ACCTAATGCT TCAACTAACT
3661 CCAGTAATTC CTTGGTGGTA CGAACATCCA ATGAAGCACA CAAGTTTGTT TGCTTTTCGT
3721 GCATGATATT AAATAGCTTG GCAGCAACAG GACTAGGATG AGTAGCAGCA CGTTCCTTAT
3781 ATGTAGCTTT CGACATGATT TATCTTCGTT TCCTGCAGGT TTTTGTTCTG TGCAGTTGGG
3841 TTAAGAATAC TGGGCAATTT CATGTTTCTT CAACACTACA TATGCGTATA TATACCAATC
3901 TAAGTCTGTG CTCCTTCCTT CGTTCTTCCT TCTGTTCGGA GATTACCGAA TCAAAAAAAT
3961 TTCAAAGAAA CCGAAATCAA AAAAAAGAAT AAAAAAAAAA TGATGAATTG AATTGAAAAG
4021 CTAGCTTATC GATGATAAGC TGTCAAAGAT GAGAATTAAT TCCACGGACT ATAGACTATA
4081 CTAGATACTC CGTCTACTGT ACGATACACT TCCGCTCAGG TCCTTGTCCT TTAACGAGGC
4141 CTTACCACTC TTTTGTTACT CTATTGATCC AGCTCAGCAA AGGCAGTGTG ATCTAAGATT
4201 CTATCTTCGC GATGTAGTAA AACTAGCTAG ACCGAGAAAG AGACTAGAAA TGCAAAAGGC
4261 ACTTCTACAA TGGCTGCCAT CATTATTATC CGATGTGACG CTGCAGCTTC TCAATGATAT
4321 TCGAATACGC TTTGAGGAGA TACAGCCTAA TATCCGACAA ACTGTTTTAC AGATTTACGA
4381 TCGTACTTGT TACCCATCAT TGAATTTTGA ACATCCGAAC CTGGGAGTTT TCCCTGAAAC
4441 AGATAGTATA TTTGAACCTG TATAATAATA TATAGTCTAG CGCTTTACGG AAGACAATGT
4501 ATGTATTTCG GTTCCTGGAG AAACTATTGC ATCTATTGCA TAGGTAATCT TGCACGTCGC
4561 ATCCCCGGTT CATTTTCTGC GTTTCCATCT TGCACTTCAA TAGCATATCT TTGTTAACGA
4621 AGCATCTGTG CTTCATTTTG TAGAACAAAA ATGCAACGCG AGAGCGCTAA TTTTTCAAAC
4681 AAAGAATCTG AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAAAGCGC TATTTTACCA
4741 ACGAAGAATC TGTGCTTCAT TTTTGTAAAA CAAAAATGCA ACGCGACGAG AGCGCTAATT
4801 TTTCAAACAA AGAATCTGAG CTGCATTTTT ACAGAACAGA AATGCAACGC GAGAGCGCTA
4861 TTTTACCAAC AAAGAATCTA TACTTCTTTT TTGTTCTACA AAAATGCATC CCGAGAGCGC
4921 TATTTTTCTA ACAAAGCATC TTAGATTACT TTTTTTCTCC TTTGTGCGCT CTATAATGCA
4981 GTCTCTTGAT AACTTTTTGC ACTGTAGGTC CGTTAAGGTT AGAAGAAGGC TACTTTGGTG
5041 TCTATTTTCT CTTCCATAAA AAAAGCCTGA CTCCACTTCC CGCGTTTACT GATTACTAGC
5101 GAAGCTGCGG GTGCATTTTT TCAAGATAAA GGCATCCCCG ATTATATTCT ATACCGATGT
5161 GGATTGCGCA TACTTTGTGA ACAGAAAGTG ATAGCGTTGA TGATTCTTCA TTGGTCAGAA
5221 AATTATGAAC GGTTTCTTCT ATTTTGTCTC TATATACTAC GTATAGGAAA TGTTTACATT
5281 TTCGTATTGT TTTCGATTCA CTCTATGAAT AGTTCTTACT ACAATTTTTT TGTCTAAAGA
5341 GTAATACTAG AGATAAACAT AAAAAATGTA GAGGTCGAGT TTAGATGCAA GTTCAAGGAG
5401 CGAAAGGTGG ATGGGTAGGT TATATAGGGA TATAGCACAG AGATATATAG CAAAGAGATA
5461 CTTTTGAGCA ATGTTTGTGG AAGCGGTATT CGCAATGGGA AGCTCCACCC CGGTTGATAA
5521 TCAGAAAAGC CCCAAAAACA GGAAGATTGT ATAAGCAAAT ATTTAAATTG TAAACGTTAA
5581 TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACGAATAGCC
5641 CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT
5701 TCCAGTTTCC AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA
5761 AAGGGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG
5821 GTCGAGGTGC CGTAAAGCAG TAAATCGGAA GGGTAAACGG ATGCCCCCAT TTAGAGCTTG
5881 ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGGGC
5941 TAGGGCGGTG GGAAGTGTAG GGGTCACGCT GGGCGTAACC ACCACACCCG CCGCGCTTAA
6001 TGGGGCGCTA CAGGGCGCGT GGGGATGATC CACTAGT
//