出品公司: | 愛迪基因 |
---|---|
載體名稱: | pLentG-KOSM |
質粒類型: | 慢病毒系統載體 |
高拷貝/低拷貝: | -- |
啟動子: | CMV |
克隆方法: | 多克隆位點,限制性內切酶 |
載體大小: | 13kb |
5' 測序引物及序列: | -- |
3' 測序引物及序列: | -- |
載體標簽: | GFP |
載體抗性: | 氨芐青霉素(Ampicillian) |
篩選標記: | -- |
備注: | -- |
產品目錄號: | -- |
穩定性: | -- |
組成型: | -- |
病毒/非病毒: | 慢病毒 |
買家導航
pLentG-KOSM載體質粒基本信息
pLentG-KOSM質粒圖譜載體圖譜和pLentG-KOSM載體序列質粒序列多克隆位點信息
pLentG-KOSM質粒載體簡介
pLentG-KOSM is a simple lentiviral vector in which defined factors are in-frame fused into a single open
reading frame (ORF) via self-cleaving 2A peptides and are controlled by a CMV promoter. The mouse
transcription factor ORF is followed by IRES-GFP as a reporter for viral transduction. This polycistronic
expression system can efficiently reprogram mouse or human somatic cells into iPS cells; for example, in
infected MEF (GFP+), the reprogramming efficiency is around 1%, which is at least 10-fold higher than
the reprogramming method using a pool of viruses in which each virus expresses a different stem cell
factor gene. Notably, most of the iPS cells generated using the pLentG-KOSM system contain only a
single copy of the viral vector (Ref. 1).
pLentG-KOSM質粒序列載體序列
pLentG-KOSM載體圖譜質粒圖譜pdf版和pLentG-KOSM載體序列質粒序列等相關資料下載
pLentG-KOSM質粒載體應用舉例
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