pMKO.1-GFP

價格：1500元

聯系方式：I47-825O-882O

買家導航

pMKO.1-GFP載體質粒基本信息

出品人:	愛迪基因
載體名稱:	pMKO.1-GFP
質粒類型:	逆病毒載體；RNAi載體
高拷貝/低拷貝:	低拷貝
克隆方法:	限制性內切酶，多克隆位點
啟動子:	U6
載體大小:	6767 bp
5' 測序引物及序列:	pLXSN 5' primer 5' CCCTTGAACCTCCTCGTTCGACC3'
3' 測序引物及序列:	pBABE 3' primer 5'ACCCTAACTGACACACATTCC3'
載體標簽:	無
載體抗性:	氨芐青霉素
篩選標記:	EGFP
克隆菌株:	DH5α、HB101等
宿主細胞（系）:	常規哺乳動物細胞系
備注:	推薦使用逆病毒包裝載體：pUMVC、pCMV-VSV-G 。
產品目錄號:	#10676
穩定性:	穩表達
組成型/誘導型:	組成型
病毒/非病毒:	逆轉錄病毒

pMKO.1-GFP質粒圖譜載體圖譜和pMKO.1-GFP載體序列質粒序列多克隆位點信息

pMKO.1-GFP載體圖譜

pMKO.1-GFP質粒載體簡介

pMKO.1-GFP質粒序列載體序列

LOCUS       pMKO.1-GFP Sequencing Result	6767 bp 	DNA	SYN
DEFINITION  pMKO.1 GFP Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="pMKO.1 GFP Sequencing Result"
                     /mol_type="other DNA"
     promoter        complement(232..434)
                     /label="SV40_promoter"
     promoter        complement(286..554)
                     /label="SV40_promoter"
     misc_feature    complement(306..325)
                     /label="SV40pro_F_primer"
     rep_origin      complement(310..387)
                     /label="SV40_origin"
     misc_feature    357..572
                     /label="SV40_enhancer"
     misc_feature    552..572
                     /label="pBABE_3_primer"
     misc_feature    708..730
                     /label="pGEX_3_primer"
     CDS             complement(1410..2129)
                     /label="ORF frame 1"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    2134..2150
                     /label="pBluescriptKS_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     promoter        complement(2154..2356)
                     /label="SV40_promoter"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     promoter        complement(2208..2476)
                     /label="SV40_promoter"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(2228..2247)
                     /label="SV40pro_F_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     rep_origin      complement(2232..2309)
                     /label="SV40_origin"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    2279..2494
                     /label="SV40_enhancer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    2474..2494
                     /label="pBABE_3_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     promoter        complement(2557..2813)
                     /label="hU6_promoter"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(2608..2627)
                     /label="LKO1_5_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(2834..3643)
                     /label="psi_plus_pack"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(2837..3244)
                     /label="gag"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(2850..2866)
                     /label="pBABE_5_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(2882..2904)
                     /label="MSCV_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(2882..2904)
                     /label="pLXSN_5_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(3713..4412)
                     /label="5_LTR"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     promoter        complement(3891..4471)
                     /label="CMV_immearly_promoter"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(3918..3938)
                     /label="CMV_fwd_primer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     misc_feature    complement(4104..4391)
                     /label="CAG_enhancer"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     promoter        4693..4721
                     /label="AmpR_promoter"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     promoter        4903..4931
                     /label="AmpR_promoter"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     gene            4973..5833
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFG
                     NQLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLR
                     YEDGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGV
                     LVGQVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQH
                     ETAIAQLTSLGKPLGSLHEWV*"
     CDS             4973..5833
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5988..6607
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 ccatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcCTTTGAGT
   61 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG
  121 CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA
  181 TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TAAGCTTTTT
  241 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA
  301 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC
  361 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA
  421 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA
  481 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG
  541 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCCGG ATCTACACTC CGCTATCGCT
  601 ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG
  661 GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT
  721 GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCCAGCTT ACCTCCCGGT
  781 GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT CCCGATCCCG GACGAGCCCC CAAATGAAAG
  841 ACCCCCGCTG ACGGGTAGTC AATCACTCAG AGGAGACCCT CCCAAGGAAC AGCGAGACCA
  901 CAAGTCGGAT GCAACTGCAA GAGGGTTTAT TGGATACACG GGTACCCGGG CGACTCAGTC
  961 AATCGGAGGA CTGGCGCCCC GAGTGAGGGG TTGTGGGCTC TTTTATTGAG CTCGGGGAGC
 1021 AGAAGCGCGC GAACAGAAGC GAGAAGCGAA CTGATTGGTT AGTTCAAATA AGGCACAGGG
 1081 TCATTTCAGG TCCTTGGGGC ACCCTGGAAA CATCTGATGG TTCTCTAGGG TCGACCCTGT
 1141 TCCATCTGTT CCTGACCTTG ATCTGAACTT CTCTATTCTC AGTTATGTAT TTTTCCATGC
 1201 CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA CAGGTGGGGT CTTTCATTCC
 1261 CCCCTTTTTC TGGAGACTAA ATAAAATCTT TTATTTTATC TATGGCTCGT ACTCTATAGG
 1321 CTTCAGCTGG TGATATTGTT GAGTCAAAAC TAGAGCCTGG ACCACTGATA TCCTGTCTTT
 1381 AACAAATTGG ACTAATCGAA GGTACCCTAT TACACCCACT CGTGCAGGCT GCCCAGGGGC
 1441 TTGCCCAGGC TGGTCAGCTG GGCGATGGCG GTCTCGTGCT GCTCCACGAA GCCGCCGTCC
 1501 TCCACGTAGG TCTTCTCCAG GCGGTGCTGG ATGAAGTGGT ACTCGGGGAA GTCCTTCACC
 1561 ACGCCCTTGC TCTTCATCAG GGTGCGCATG TGGCAGCTGT AGAACTTGCC GCTGTTCAGG
 1621 CGGTACACCA GGATCACCTG GCCCACCAGC ACGCCGTCGT TCATGTACAC CACCTCGAAG
 1681 CTGGGCTGCA GGCCGGTGAT GGTCTTCTTC ATCACGGGGC CGTCGTTGGG GAAGTTGCGG
 1741 CCCTTGTACT CCACGCGGTA CACGAACATC TCCTCGATCA GGTTGATGTC GCTGCGGATC
 1801 TCCACCAGGC CGCCGTCCTC GTAGCGCAGG GTGCGCTCGT ACACGAAGCC GGCGGGGAAG
 1861 CTCTGGATGA AGAAGTCGCT GATGTCCTCG GGGTACTTGG TGAAGGTGCG GTTGCCGTAC
 1921 TGGAAGGCGG GGCTCAGGAT GTCGAAGGCG AAGGGCAGGG GGGCGCCCTT GGTCACGCGG
 1981 ATCTGCACCA GCTGGTTGCC GAACAGGATG TTGCCCTTGC CGCAGCCCTC CATGGTGAAC
 2041 ACGTGGTTGT TCACCACGCC CTCCAGGTTC ACCTTGAAGC TCATGATCTC CTGCAGGCCG
 2101 GTGTTCTTCA GGATCTGCTT GCTCACCATG GTCTCGAGGT CGACGGTATC GATAAGCTTT
 2161 TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC
 2221 GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG
 2281 GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT
 2341 GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA
 2401 CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT
 2461 GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGGG TCGACCACTG TGCTGGCGAA
 2521 TTCCATATGG GAGTACTAGT ACGCGTACCG GTGTTTCGTC CTTTCCACAA GATATATAAA
 2581 GCCAAGAAAT CGAAATACTT TCAAGTTACG GTAAGCATAT GATAGTCCAT TTTAAAACAT
 2641 AATTTTAAAA CTGCAAACTA CCCAAGAAAT TATTACTTTC TACGTCACGT ATTTTGTACT
 2701 AATATCTTTG TGTTTGCAGT CAAATTAATT CTAATTATCT CTCTAACAGC CTTGTATCGT
 2761 ATATGCAAAT ATGAAGGAAT CATGGGAAAT AGGCCCTCTT CCTGCCCGAC CTTGTCGACA
 2821 GATCTTCAAT TCCGGCGCCT AGAGAAGGAG TGAGGGCTGG ATAAAGGGAG GATCGAGGCG
 2881 GGGTCGAACG AGGAGGTTCA AGGGGGAGAG ACGGGGCGGA TGGAGGAAGA GGAGGCGGAG
 2941 GCTTAGGGTG TACAAAGGGC TTGACCCAGG GAGGGGGGTC AAAAGCCAAG GCTTCCCAGG
 3001 TCACGATGTA GGGGACCTGG TCTGGGTGTC CATGCGGGCC AGGTGAAAAG ACCTTGATCT
 3061 TAACCTGGGT GATGAGGTCT CGGTTAAAGG TGCCGTCTCG CGGCCATCCG ACGTTAAAGG
 3121 TTGGCCATTC TGCAGAGCAG AAGGTAACCC AACGTCTCTT CTTGACATCT ACCGACTGGT
 3181 TGTGAGCGAT CCGCTCGACA TCTTTCCAGT GACCTAAGGT CAAACTTAAG GGAGTGGTAA
 3241 CAGTCTGGCC CTAATTTTCA GACAAATACA GAAACACAGT CAGACAGAGA CAACACAGAA
 3301 CGATGCTGCA GCGCTGCAGC AGACAAGACG CGCGGCTTCG GTTCCAAACC GAAAGCAAAA
 3361 ATTCAGACGG AGGCGGGAAC TGTTTTAGGT TCTCGTCTCC TACCAGAACC ACATATCCTG
 3421 ACGGGGTCGG ATTCCACATC GACTCCCTTC CTCAGGTCGG GCCACAAAAA CGGCCCCCAA
 3481 AGTCCCTGGG ACGTCTCCCA GGGTTGCGGC CGGGTGTTCA GAACTCGTCA GTTCCACCAC
 3541 GGGTCCGCCA GATACAGAGC TAGTTAGCTA ACTAGTACAG ACGCAGGCGC ATAACATCAA
 3601 ACATAGACAC TAGACAATCG GACAGACACA GATAAGTTGC TGGCCAGCTT ACCTCCCGGT
 3661 GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT CCAAATCCCG GACGAGCCCC CAAATGAAAG
 3721 ACCCCCGTCG TGGGTAGTCA ATCACTCAGA GGAGACCCTC CCAAGGAACA GCGAGACCAC
 3781 GATTCGGATG CAAACAGCAA GAGGCTTTAT TGGGAATACG GGTACCCGGG CGACGCAGTC
 3841 TATCGGAAGA CTGGCGCGCC GAGTGAGGGG TTGTGGGCTC TTTTATTGAG CTCTGCTTAT
 3901 ATAGACCTCC CACCGTACAC GCCTACCGCC CATTTGCGTC AATGGGGCGG AGTTGTTACG
 3961 ACATTTTGGA AAGTCCCGTT GATTTTGGTG CCAAAACAAA CTCCCATTGA CGTCAATGGG
 4021 GTGGAGACTT GGAAATCCCC GTGAGTCAAA CCGCTATCCA CGCCCATTGA TGTACTGCCA
 4081 AAACCGCATC ACCATGGTAA TAGCGATGAC TAATACGTAG ATGTACTGCC AAGTAGGAAA
 4141 GTCCCATAAG GTCATGTACT GGGCATAATG CCAGGCGGGC CATTTACCGT CATTGACGTC
 4201 AATAGGGGGC GTACTTGGCA TATGATACAC TTGATGTACT GCCAAGTGGG CAGTTTACCG
 4261 TAAATACTCC ACCCATTGAC GTCAATGGAA AGTCCCTATT GGCGTTACTA TGGGAACATA
 4321 CGTCATTATT GACGTCAATG GGCGGGGGTC GTTGGGCGGT CAGCCAGGCG GGCCATTTAC
 4381 CGTAAGTTAT GTAACGCGGA ACTCCATATA TGGGCTATGA ACTAATGACC CCGTAATTGA
 4441 TTACTATTAA TAACTAGTCA ATAATCAATG TCAACATGGC GGTAATGTTG GACATGAGCC
 4501 AATATAAATG TACATATTAT GATATGGATA CAACGTATGC AATGGCCAAG CTAATTCTTG
 4561 AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT
 4621 TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT
 4681 TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA
 4741 ATAATTAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA
 4801 AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TTAGGTGGCA CTTTTCGGGG
 4861 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT
 4921 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT
 4981 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC
 5041 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG
 5101 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG
 5161 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA
 5221 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA
 5281 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC
 5341 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC
 5401 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG
 5461 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC
 5521 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA
 5581 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT
 5641 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT
 5701 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG
 5761 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT
 5821 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT
 5881 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT
 5941 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC
 6001 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT
 6061 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG
 6121 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA
 6181 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC
 6241 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA
 6301 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC
 6361 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA
 6421 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG
 6481 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG
 6541 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG
 6601 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC
 6661 TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC
 6721 TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAG
//

pMKO.1-GFP載體圖譜質粒圖譜pdf版和pMKO.1-GFP載體序列質粒序列等相關資料下載

pMKO.1-GFP質粒載體應用舉例

上一篇：pBABE-puro-IRES-EGFP
下一篇：pMKO.1-puro

pcDNA6-Flag-N/Myc-His	pHIS1525
pHY300PLK	pGL4.24[luc2P/minP]
pMKS-1	pDsRed2-Bid
pLXIN	pmR-mCherry
pCXGFP-P-pXcmI-GFP	pCRII-TOPO/-17-23
pCas	pEMBLYe30
pRI201-ON	pDONR221-P5P2
pLAFR1	pJW342
pCold III	pSos-MAFB
pCambia0390	pBABE-hygro
pGBKT7-Lam	pCMV-LacZ
pCX-DR	pmCerulean-N1
pGT12	pGEc41
pAX01-ComK	pCMV-tdTomato
pLKO.3G	pGL4.32[luc2P/NF-κB-RE/Hygro]
pLenti6.4/R4R2/V5-DEST	pCEP4-Flag-N
pLP/VSVG	pRB394
pcDNA3.1/GS	pLF6
pCGN978	pSW2
pCSN43	pcDNA4/TO/Myc-His C
pCMV-3Tag-1a-P2A	pIJ702-36Y
pTYB1	pMMB207 sp.
pRSVADH	PX330S-2
pSP72	pMCSG7
pTP-5	ColE1
pCMV-VSVG	pCR14-18
pFN10A (ACT)	PinPoint Xa-2
HDM-Rev1b	pAG60
pTP-4	pEF4/myc-His B
pQCXIX	pBE2RS(pBE2-R)
pLL3.7	pCMV-CREB
pCold I	TEV protease
pEXP1-DEST	pCAMBIA1105.1
pDEST32	pDNAPolbeta_TA
pMET C	pET302/NT-His
pED-Trx-pp-air	pAdTrack
pCMV-Gag-Pol	pOG44
pTRP-H3	pHBLV-CMV-MCS-IRES-Puro
pRluc-N1	pCambia2200
pGL4.84[hRlucCP/Puro]	pMG36e
pTRE3G-Luc	pISRE-TA-Luc
pRtr5a::Tn5	pECFP-ER
pDE3	pVK102
pAAV-hSyn-RFP	SARS Frag. 14 pCRII-TOPO
pGL4GH43-TA	pUT-mini-Tn5-Km
pmGeos-M-C1	Super1300
pSK1	pSAM2
pUB6/V5 His lacZ	P416GPD
pcDNA3.1/Myc-His B	pET-24d(+)
YIplac204	pBBR1MCS2-pAmp-EGFP

pCAG-T7pol	pCMV-PE2
pAnc80L65	pBiFC-bFOSDelataZipVC155
ABE8.8-m	pLVX-EF1alpha-SARS-CoV-2-nsp1-2xStrep-IR
pC0061 PsmCas13 (B05) His6-TwinStrep-SUM	pGreenIIM DR5v2-ntdTomato/DR5-n3GFP
HA-Rab8a-CA (Q67L)	pBMTBX-3
pQCascade	EGFP-Rab7A Q67L
pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-	BECLIN-HA WT
pDHt-SK	pX330a dCas9-KRAB
pHR_5x Gal4 UAS	pMSCV-IRES-DsRed FP (pMIDsRed II)
pBiFC-VN155(I152L)	pcDNA3 Flag-humanTRAF6 (1093)
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE	pEF1a-BirA-V5-neo
pCMT-flp	HA-Rab8a-DN (T22N)
pGD-p19	pNG6
pRADZ1	pNH6
pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-R	pMal-T-Avi-His/BirA
SP-dCas9-VPR	EC.SurA.pTYB1.a21-281&390-428.wt
pLV-CMV-LoxP-DsRed-LoxP-eGFP	pXR002:EF1a-dCasRx-2A-EGFP
pMPRA1	pMXs-Lhx3
pFLP-hyg	ERM-APEX2
pmRFP_LAP2beta_IRES_puro2b	vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Fla
pClneoHA mouse Numb	pRK5-FLAG-SLC38A9.1
pcDNA3/Flag-METTL14	pLenti CMV/TO p16 Hygro (w184-1)
pCgsgRNA_crtYf	pEGFP-LC3
pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_	pSLQ1651-sgTelomere(F+E)
pHAGE_puro	pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-I
pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs	pPH37
p75-RFP	S2060
pQCascade-tra13	pcDNA3-H-Ras_V12
hRad51(K133R)–Cas9(D10A)	pMGS56(GFP-ARF16-PB1-P2A-OsTIR1)
pHAGE-EFS-MCP-3XBFPnls	MCP-mCherry-SEC61B
pEJS578_DD-dSpyCas9-mCherry-APEX2	pLEX_305-C-dTAG
pT7-NSP3SA11	HER2 CA (V659E)
pST1374-scFv-APOBEC-UGI-GB1	pSR660
pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/	G1397 DddAtox-N–SaKKH-Cas9(D10A)
p45sheLL	pNN6
FUW-tetO-lox-hSOX2	mCherry-Parkin
pCW57-MCS1-P2A-MCS2 (Neo)	pCNA
pcDNA-K-GECO1	pKA-142
pCMV-dCas13-M3M14nes	pFUPI hT5-S322-Cyp
pAnc80L65AAP	pHR_PGK_antiCD19_synNotch_Gal4VP64
pFastBac His6 TEV cloning vector with Bi	xCas9 3.6
pEGFP(C3)-Nup153	pQUAST>stop>mCD8-GFP
pSpCas9(BB)-2A-Puro (PX459)	pET-NLS-Cas9-6xHis
DNMT1 KO Hyg	pMA-SpCas9-g6
pNN1	pLVX-Tet3G blasticidin
pMOD_C2515	pcDNA3.1(+) CircRNA Mini Vector
pGST-PPARgamma	pMKO.1 GFP
lenti dCAS-VP64_Blast	CAMK2D gRNA (BRDN0001146112)
pCE-mp53DD	pJK-caCas9-NatMX-Neut5L-Ade2 drive
pHAGE2-TetOminiCMV-SKM	pBabepuro-KIBRA

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