LOCUS       pDC512       3277 bp dsDNA    Circular
DEFINITION  Puc based frt  shuttle plasmid  for FLP-based Ad vector rescue Cummings & Graham, unpublished
COMMENT     This file is best viewed with a free Vector NTI Viewer:
COMMENT     //www.informaxinc.com/view/viewer.html
COMMENT     VNTREPLTYPE|Plasmid|
FEATURES               Location/Qualifiers
     rep_origin      967..1267
                     /label=ori
     CDS             complement(1717..2533)
                     /label=amp
     misc_feature    2818..453
                     /label=Ad
     misc_feature    509..542
                     /label=Frt
     CDS             3187..1
                     /label=ITR
     CDS             complement(2..102)
                     /label=ITR
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 
      421 gggtcaaagt tggcgtttta ttattatagt cagctctaga gtcgacgagc tcaagcttag 
      481 atctgctagc aggcctgaat tcggatccga agttcctatt cttactagag tataggaact 
      541 tcgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg 
      601 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc 
      661 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt 
      721 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccctgcat 
      781 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 
      841 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 
      901 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 
      961 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
     1021 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
     1081 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
     1141 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
     1201 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
     1261 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
     1321 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 
     1381 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 
     1441 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 
     1501 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 
     1561 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 
     1621 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 
     1681 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 
     1741 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 
     1801 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 
     1861 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 
     1921 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 
     1981 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 
     2041 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 
     2101 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 
     2161 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 
     2221 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 
     2281 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 
     2341 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc 
     2401 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 
     2461 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 
     2521 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 
     2581 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 
     2641 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 
     2701 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 
     2761 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcactcta 
     2821 ggcaaaatag caccctcccg ctccagaaca acatacagcg cttcacagcg gcagcctaac 
     2881 agtcagcctt accagtaaaa aagaaaacct attaaaaaaa caccactcga cacggcacca 
     2941 gctcaatcag tcacagtgta aaaaagggcc aagtgcagag cgagtatata taggactaaa 
     3001 aaatgacgta acggttaaag tccacaaaaa acacccagaa aaccgcacgc gaacctacgc 
     3061 ccagaaacga aagccaaaaa acccacaact tcctcaaatc gtcacttccg ttttcccacg 
     3121 ttacgtaact tcccatttta agaaaactac aattcccaac acatacaagt tactccgccc 
     3181 taaaacctac gtcacccgcc ccgttcccac gccccgcgcc acgtcacaaa ctccaccccc 
     3241 tcattatcat attggcttca atccaaaata aggtata 
//