| 出品公司: | Agilent |
|---|---|
| 載體名稱: | pShuttle-IRES-hrGFP-1 |
| 質粒類型: | 腺病毒表達載體，哺乳動物細胞表達載體 |
| 高拷貝/低拷貝: | 低拷貝 |
| 啟動子: | CMV |
| 克隆方法: | 多克隆位點，限制性內切酶 |
| 載體大小: | 8.9 kb |
| 5' 測序引物及序列: | pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC |
| 3' 測序引物及序列: | pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG |
| 載體標簽: | -- |
| 載體抗性: | 卡那 |
| 備注: | 載體內包含有IRES序列和hrGFP序列。 |
| 產品目錄號: | 240081 |
| 穩定性: | / |
| 組成型: | -- |
| 病毒/非病毒: | 腺病毒 |
買家導航
pShuttle-IRES-hrGFP-1載體質粒基本信息
pShuttle-IRES-hrGFP-1質粒圖譜載體圖譜和pShuttle-IRES-hrGFP-1載體序列質粒序列多克隆位點信息
pShuttle-IRES-hrGFP-1質粒載體簡介
Two additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG® epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).