| 出品公司: | TAKARA |
|---|---|
| 載體名稱: | pRI101-AN |
| 質粒類型: | 植物載體；農桿菌雙元表達載體 |
| 高拷貝/低拷貝: | 高拷貝 |
| 克隆方法: | 限制性內切酶，多克隆位點 |
| 啟動子: | 35S |
| 載體大小: | 10417 bp |
| 5' 測序引物及序列: | 35S promoter 5’CTATCCTTCGCAAGACCCTTC 3’ |
| 3' 測序引物及序列: | M13 Reverse 5’CAGGAAACAGCTATGAC 3’ |
| 載體標簽: | 無 |
| 載體抗性: | 卡那霉素 |
| 篩選標記: | 卡那霉素 |
| 克隆菌株: | HB101 |
| 宿主細胞（系）: | 植物細胞、農桿菌 |
| 備注: | pRI101-AN載體是一種在雙子葉植物細胞中表達外源基因的載體。利用發根土壤桿菌介導。 |
| 產品目錄號: | 3262 |
| 穩定性: | 穩表達 |
| 組成型/誘導型: | 組成型 |
| 病毒/非病毒: | 非病毒 |
買家導航
pRI101-AN載體質粒基本信息
pRI101-AN質粒圖譜載體圖譜和pRI101-AN載體序列質粒序列多克隆位點信息
pRI101-AN質粒載體簡介
pRI101-AN載體簡介：
pRI101 DNA vectors are binary plant transformation vectors intended for foreign gene
expression in plant cells. These agrobacterium-mediated plant transformation vectors carry
the 35S promoter of caμliflower mosaic virus (CaMV) and the 5’ non-coding region (5’-UTR)
of the alcohol dehydrogenase (ADH) gene. The 5’-UTR of ADH functions as a translation
enhancer in plants. There are two types of pRI101 DNA vectors, the pRI101-AN DNA vector,
which carries the 5’-UTR of Arabidopsis ADH (AtADH 5’-UTR), and the pRI101 ON DNA vector,
which carries the 5’-UTR of rice ADH (OsADH 5’-UTR). The pRI101-AN DNA vector is for
dicotyledonous plants such as tobacco or Arabidopsis while the pRI101-ON DNA vector is for
monocotyledonous plants such as rice.
Although the pRI101 DNA vectors are shuttle vectors and replicate autonomously in E. coli and
Rhizobium (Agrobacterium), they are high copy number plasmids because they contain the same
replication origin as pUC-type plasmids (ColE1 ori). These vectors are also stably maintained
in Rhizobium (Agrobacterium) containing the mutant-type replication origin Ri (Ri-ori). The
pRI101 DNA vectors are capable of stably integrating target genes into plant chromosomes
because the vector cloning sites are located closer to the Right Border (RB) of T-DNA than
the selection marker (NPT II) of the plant, so the target gene is not deleted.
pRI101-AN載體特征：
Agrobacterium-mediated plant transformation
Easy and stable integration of dicot and monocot target genes into plant chromosomes
Increased target gene expression via the 5'-UTR translation enhancer region
Vector versatility for dicotyledonous and monoctyledonous plant cells
參考文獻：
1. Sugio, T., et al. (2008) J. Bioscience and Bioengineering 105(3):300-302.
2. Nishiguchi, R. et al. (1987) Mol. and Gen. Genet. 206:1-8.
3. Satoh, J. et al. (2004) J. Bioscience and Bioengineering 98(1):1-8.
pRI101-AN質粒序列載體序列
TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTC CATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGA AACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCT CCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTG GCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTAT CGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAAC AGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAAC TACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTC GGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATC TTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATG AGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCA ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCA CCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCGATCCTACAAGG TAGAATCCGCCTGAGTCGCAAGGGTGACTTCGCCTATATTGGACGACGGCGCGCAGAGGG CGACCTCTTTTTGGGTTACGATTGTAGGATTATCACTAAAACAATACATGAACATATTCA AATGGCAATCTCTCTAAGGCATTGGAAATAAATACAAATAACAGTTGGGTGGAGTTTTTC GACCTGAGGGCGTTAACCTTCTGTTAACCTAAAAGCTCTTGCCCAAACAGCAGAATCGGC GCTAATTGCCAGCGGCGGAACTTTTCCAGTTTCGCGAAAAATATCGCCACTGGCAAGGAA TGGGTTTGAGATGGCGAAGTCTGTCCTAAAAGCAGCGCCTGTAGTTGTAGGGTTGACGGC CTTGATGGAGCGTCATGCCGATGCCCTCTCGAGCCAACTTCAAGCACATCATCTTAAGGT TTTCCCGCCGCATTCCGAGAAGGGTATTCGAACATTCGGGCCATCGGAGGCGTCCAAGCT GCTCGGCGTTGGCGAGTCATATTTACGGCAGACCGCGTCTGAGATGCCAGAGTTGAATGT TAGCATGAGCCCAGGTGGCAGGCGAATGTTCTCAATTGAAGATATCCATGTGATTCGGAA GTATATGGATCAGGTCGGCCGCGGGAACCGGCGCTACCTGCCACATCGTCGAGGCGGCGA GCAGCTTCAGGTTATCTCTGTGATGAATTTCAAAGGTGGGTCGGGTAAGACCACCACCGC CGCGCATCTGGCGCAGTACCTCGCTATGCGCGGATATCGAGTCTTGGCCATTGATCTCGA TCCTCAAGCGAGCCTTTCTGCACTCTTTGGGAGCCAACCGGAGACGGACGTTGGCCCGAA CGAAACGCTCTACGGCGCTATAAGGTATGATGATGAGCAGGTGGCAATCGAACGAGTCGT CCGAGGGACTTACATTCCCGACCTCCACCTGATTCCTGGTAACCTTGAGCTGATGGAGTT TGAACACGATACGCCACGCGCGCTGATGAACCGCAAAGAGGGCGACACGCTCTTTTATGG TCGCATCAGCCAAGTAATTGAAGATATCGCGGATAACTATGACGTCGTGGTCATCGACTG CCCTCCCCAGCTTGGGTATCTCACGCTATCCGCATTGACTGCGGCGACGTCCATTCTTGT CACGGTCCATCCGCAGATGCTGGATGTGATGTCGATGAACCAGTTTCTGGCAATGACATC GAACCTTTTGCGTGAAATCGAGAATGCTGGCGCCAAGTTCAAGTTTAATTGGATGCGCTA TCTGATAACCCGTTTCGAACCGAGCGACGGACCACAGAACCAAATGGTAGGTTATCTGCG GTCGATTTTTGGCGAAAATGTCCTCAATTTTCCGATGCTTAAAACCACCGCGGTTTCGGA CGCTGGCCTGACAAACCAGACTCTATTCGAAGTGGAGCGTGGCCTGTTCACGCGCTCGAC CTATGATCGAGCCTTGGAGGCGATGAACGCCGTCAACGACGAGATCGAAACACTGATCAA AAAAGCATGGGGTAGGCCCACATGAGCCGGAAGCACATCCTTGGCGTCTCAACTGACGCC CCTGAGACGTCGCCCGCCGACAATAGGACGGCAAAGAACCGCTCCATGCCGCTCCTCGGC GTAACAAGGAAGGAGCGCGATCCGGCAACGAAGCTCACAGCGAACATTGGTAACGCACTG CGAGAGCAAAACGATCGTCTTAGCCGTGCCGAAGAGATCGAGCGGCGTCTCGCTGAAGGT CAGGCAGTGATAGAGTTGGATGCCTCGTCAATAGAACCGTCTTTCGTGCAGGATCGTATG CGAGGGGACATTGACGGGCTCCTTACTTCGATCCGGGAACAAGGACAGCAAGTCCCAATC CTTGTGCGACCGCATCCGAGCCAGCCGGGCCGATATCAGGTTGCCTTCGGCCACCGCCGG CTACGCGCCGTTTCAGAACTCGGACTTCCGGTCAGGGCGGTCGTTCGCGAACTGACGGAC GAGCAAGTGGTCGTAGCACAGGGTCAGGAAAACAATGTGCGCGAAGATCTTACCTTCATC GAAAAGGCGCGCTTCGCACATCGCCTGAACAGGCAGTTTTCTCGAGAGATTGTCATCGCC GCGATGTCGATCGACAAGAGCAATTTGTCCAAGATGCTTCTGCTCGTTGACGCCCTCCCC TCTGAACTGACCGATGCTATTGGTGCCGCTCCTGGTGTTGGACGGCCGAGTTGGCAACAA CTTGCCGAGCTGATTGAGAAAGTTTCTTCACCGGCCGACGTGGCTAAATATGCTATGTCG GAGGAAGTTCAAGCGCTGCCATCGGCAGAACGATTCAAGGCGGTGATCGCTAGTCTGAAG CCCAGTCGGGTTGCGCGTGGACTTCCCGAGGTCATGGCCACCCCAGACGGCACCAGAATT GCACAGGTGACGCAGAGCAAGGCCAAACTGGAAATCACGATTGACAGGAAGGCGACGCCC GATTTTGCGACCTTCGTGCTCGATCATGTGCCAGCGCTGTATCAAGCGTACCACGCTGAG AACCAACGGAAACGGGGAGAGTAAACCGCAAAAGAAAAGAGCCCCCTCAACGTCGCCGTC GCGGAAGCCCTTCTGTCTCTCTAGCGCGAACAGAATCGCATTTCCTCGAATCCTCGTCAA GAGTTTTTAGCGCCGTTTTGGTGAGCTGATTTCCTTTGCCTGCTGAAAGGTGAAAGATGA TGCAGACAGGAAGTGTAACGACGCCATTCGGGCGGCGGCCAATGACGCTTGCGCTTGTGC GGCGCCAGACGGCGCTGGCCGATATCAAACAAGGCAAGACAGCGGACAAGTGGAAGGTCT TTAGGGACGCGTCCGCGGCCATGGAACTACTTGGAATCCAGTCCAACAGTCTTGCCGTCC TTGATGCGCTATTGAGCTTTCACCCGGAAACGGAGTTGCGTCAGGAGGCACAGCTGATCG TCTTCCCGTCGAATGCTCAGCTTGCCCTTCGGGCGCATGGGATGGCTGGCGCGACTTTGC GTAGGCACATCGCCATGCTCGTGGAGTCAGGCTTGATCGTCCGGAAGGATAGCGCCAACG GAAAGCGTTACGCTCGTAAGGATGGCGCTGGTCAGATCGAGCGCGCGTTTGGCTTCGATT TGTCTCCGCTTCTCGCGCGGTCCGAAGAGCTAGCGATGATGGCACAGCAGGTGATGGCCG ATCGAGCAGCATTCAGGATGGCCAAAGAAAGTCTGACGATTTGCCGACGGGACGTTCGGA AGCTAATTACGGCAGCTATGGAAGAGGGAGCGGAGGGCGACTGGCAAGCTGTCGAGGAAG TCTATGTGGAACTTGTGGGTAGAATCCCACGCGCCCCGACGCTTGCTGATGTAGAGTCAA TTCTCGAAGAGATGTGGATGCTCCAGGAAGAGATAATCAACCGGTTGGAAATTAGAGACA ATTCAGAAAATAATAGCACCAATGCTGCCCAGAGCGAGCAGCACATACAGAACTCAAAAC CCGAATCCGTTAATGAACTTGAACCTCGCTCTGAAAAGGAGCAGGGCGCTAAGCCGAGTG AAATAGACCGGGCAAGGAGCGAGCCGATAAAAGCGTTCCCCCTCGGGATGATCCTGAAAG CGTGCCCGACCATTGGCAATTATGGGCCGAGCGGTGCGGTTGCTAGCTGGCGTGACCTCA TGTCGGCTGCGGTGGTGGTTCGGTCTATGCTGGGGGTCAGCCCGTCGGCTTACCAAGACG CGTGTGAGGCAATGGGACCGGAGAATGCGGCAGCAGCGATGGCGTGCATTTTGGAGCGAG CGAACTTCATCAATTCGCCTGGGGGCTATCTCCGAGATCTGACACGGCGGAGCGAACTCG GGAAGTTTTCACTTGGCCCGATGATAATGGCGCTCTTGAAGGCTAGCGGGCAGGGGACGT TGCGGTTTGGCTAGAATTAGCGAGTATGGAGCAGGATGGTCTGTGGTCAGCTGACCACAG ACCTAATAGGTTGAAAACATGAGCGTTTTTTGGATGATCGACAGACCATCCGATTCCCGG AGTACCAAGCGTGCTCTGATGGGAGCGATAACATTACTCAACAAGCACGAAGGCCCCATG CCGATCGTTGATCGTGAAGGAGAGCCTGCTCTACATGCGGCGGTATTTTGCCGGCCGAGG CATGTAGTCGCGGAGCACTGCCTATTTACTGCCCTAGGCACAAACGTTGACTCTTGGATC GAGCTGGCAGACAAAGCAATAACCCACACAGAGGACGATTAATGGCTGACGAAGAGATCC AGAATCCGCCGGACGGTACTGCTGCTGCCGAAGTTGAGCCGGCTGCTCCTAGAGGTAGAA GAGCAAAGAAAGCACCAGCCGAAACAGCCCGCACGGGATCGTTCAAATCCGTGAAGCCGA AAACCCGCGGCCTCAGCAACCGAGAAAAACTGGAGAAGATCGGTCAAATCGAAGCTCAGG TCGCTGGCGGCGCAACCTTGAAGGACGCCGTTAAGATCGTGGGTATTTCCGTTCAGACCT ATTATCAATGGAAGAGAGCTGCGGTTCAACCTGTCTCACAGAATCCGGCCGTGTCTGTTT CAGTTGACGATGAACTCGGCGAGTTCATCCAACTCGAGGAGGAAAATCGGCGGCTCAGAA AGCTAGCTTGTGGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATA ACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGC AACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTG GTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAATCCCTT ATAAATCAAAAGAATAGCCCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTC CACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATG GCCCAAATTCCCGATCTAGTAACATAGATGACACCGCGCGCGATAATTTATCCTAGTTTG CGCGCTATATTTTGTTTTCTATCGCGTATTAAATGTATAATTGCGGGACTCTAATCATAA AAACCCATCTCATAAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAAT TCAACAGAAATTATATGATAATCATCGCAAGACCGGCAACAGGATTCAATCTTAAGAAAC TTTATTGCCAAATGTTTGAACGATCGGGGCCCGATCATATTGTCGCTCAGGATCGTGGCG TTGTGCTTGTCGGCCGTTGCTGTCGTAATGATATCGGCACCTTCGACCGCCTGTTCCGCA GAGATCCCGTGGGCGAAGAACTCCAGCATGAGATCCCCGCGCTGGAGGATCATCCAGCCG GCGTCCCGGAAAACGATTCCGAAGCCCAACCTTTCATAGAAGGCGGCGGTGGAATCGAAA TCTCGTGATGGCAGGTTGGGCGTCGCTTGGTCGGTCATTTCGAACCCCAGAGTCCCGCTC AGAAGAACTCGTCAAGAAGGCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGGCGATAC CGTAAAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGCAATATCACGGG TAGCCAACGCTATGTCCTGATAGCGGTCCGCCACACCCAGCCGGCCACAGTCGATGAATC CAGAAAAGCGGCCATTTTCCACCATGATATTCGGCAAGCAGGCATCGCCATGGGTCACGA CGAGATCATCGCCGTCGGGCATACGCGCCTTGAGCCTGGCGAACAGTTCGGCTGGCGCGA GCCCCTGATGCTCTTCGTCCAGATCATCCTGATCGACAAGACCGGCTTCCATCCGAGTAC GTGCTCGCTCGATGCGATGTTTCGCTTGGTGGTCGAATGGGCAGGTAGCCGGATCAAGCG TATGCAGCCGCCGCATTGCATCAGCCATGATGGATACTTTCTCGGCAGGAGCAAGGTGAG ATGACAGGAGATCCTGCCCCGGCACTTCGCCCAATAGCAGCCAGTCCCTTCCCGCTTCAG TGACAACGTCGAGCACAGCTGCGCAAGGAACGCCCGTCGTGGCCAGCCACGATAGCCGCG CTGCCTCGTCCTGTAGTTCATTCAGGGCACCGGACAGGTCGGTCTTGACAAAAAGAACCG GGCGCCCCTGCGCTGACAGCCGGAACACGGCGGCATCAGAGCAGCCGATTGTCTGTTGTG CCCAGTCATAGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATCCAT CTTGTTCAATCATGCGAAACGATCCAGATCCGGTGCAGATTATTTGGATTGAGAGTGAAT ATGAGACTCTAATTGGATACCGAGGGGAATTTATGGAACGTCAGTGGAGCATTTTTGACA AGAAATATTTGCTAGCTGATAGTGACCTTAGGCGACTTTTGAACGCGCAATAATGGTTTC TGACGTATGTGCTTAGCTCATTAAACTCCAGAAACCCGCGGCTGAGTGGCTCCTTCAACG TTGCGGTTCTGTCAGTTCCAAACGTAAAACGGCTTGTCCCGCGTCATCGGCGGGGGTCAT AACGTGACTCCCTTAATTCTCCGCTCATGATCCTGGCACGACAGGTTTCCCGACTGGAAA GCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCT TTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCAC ACAGGAAACAGCTATGACCATGATTACGAATTAATTCCCGATCTAGTAACATAGATGACA CCGCGCGCGATAATTTATCCTAGTTTGCGCGCTATATTTTGTTTTCTATCGCGTATTAAA TGTATAATTGCGGGACTCTAATCATAAAAACCCATCTCATAAATAACGTCATGCATTACA TGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGAC CGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGATCGGGGAAAT TCGAGCTCGGTAGCAATTCCCGAGGCTGTAGCCGACGATGGTGCGCCAGGAGAGTTGTTG ATTCAGAATTCGGATCCGGTACCCCCGGGGTCGACGGGCATATGTATCAACAGTGAAGAA CTTGCTTTTGATCTTTTGTTAGTTTTGTGTGATTGTGATGTATCTAGAGTCCCCCGTGTT CTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGAT TGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTG GTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTTGGGACCA CTGTCGGCAGAGGCATCTTCAACGATGGCCTTTCCTTTATCGCAATGATGGCATTTGTAG GAGCCACCTTCCTTTTCCACTATCTTCACAATAAAGTGACAGATAGCTGGGCAATGGAAT CCGAGGAGGTTTCCGGATATTACCCTTTGTTGAAAAGTCTCAATTGCCCTTTGGTCTTCT GAGACTGTATCTTTGATATTTTTGGAGTAGACAAGTGTGTCGTGCTCCACCATGTTGACG AAGATTTTCTTCTTGTCATTGAGTCGTAAGAGACTCTGTATGAACTGTTCGCCAGTCTTT ACGGCGAGTTCTGTTAGGTCCTCTATTTGAATCTTTGACTCCATGGCCTTTGATTCAGTG GGAACTACCTTTTTAGAGACTCCAATCTCTATTACTTGCCTTGGTTTGTGAAGCAAGCCT TGAATCGTCCATACTGGAATAGTACTTCTGATCTTGAGAAATATATCTTTCTCTGTGTTC TTGATGCAGTTAGTCCTGAATCTTTTGACTGCATCTTTAACCTTCTTGGGAAGGTATTTG ATTTCCTGGAGATTATTGCTCGGGTAGATCGTCTTGATGAGACCTGCTGCGTAAGCCTCT CTAACCATCTGTGGGTTAGCATTCTTTCTGAAATTGAAAAGGCTAATCTGGGGACCTGCA GGCATGCAAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCG TTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGGATCAGATTGTCGTTTC CCGCCTTCAGTTTAAACTATCAGTGTTTGACAGGATATATTGGCGGGTAAACCTAAGAGA AAAGAGCGTTTATTAGAATAATCGGATATTTAAAAGGGCGTGAAAAGGTTTATCCGTTCG TCCATTTGTATGTGACTAAAACAATTCATCCAGTAAAATATAATATTTTATTTTCTCCCA ATCAGGCTTGATCCCCAGTAAGTCAAAAAATAGCTCGACATACTGTTCTTCCCCGATATC CTCCCTGATCGACCGGACGCAGAAGGCAATGTCATACCACTTGTCCGCCCTGCCGCTTCT CCCAAGATCAATAAAGCCACTTACTTTGCCATCTTTCACAAAGATGTTGCTGTCTCCCAG GTCGCCGTGGGAAAAGACAAGTTCCTCTTCGGGCTTTTCCGTCTTTAAAAAATCATACAG CTCGCGCGGATCTTTAAATGGAGTGTCTTCTTCCCAGTTTTCGCAATCCACATCGGCCAG ATCGTTATTCAGTAAGTAATCCAATTCGGCTAAGCGGCTGTCTAAGCTATTCGTATAGGG ACAATCCGATATGTCGATGGAGTGAAAGAGCCTGATGCACTCCGCATACAGCTCGATAAT CTTTTCAGGGCTTTGTTCATCTTCATACTCTTCCGAGCAAAGGACGCCATCGGCCTCACT CATGAGCAGATTGCTCCAGCCATCATGCCGTTCAAAGTGCAGGACCTTTGGAACAGGCAG CTTTCCTTCCAGCCATAGCATCATGTCCTTTTCCCGTTCCACATCATAGGTGGTCCCTTT ATACCGGCTGTCCGTCATTTTTAAATATAGGTTTTCATTTTCTCCCACCAGCTTATATAC CTTAGCAGGAGACATTCCTTCCGTATCTTTTACGCAGCGGTATTTTTCGATCAGTTTTTT CAATTCCGGTGATATTCTCATTTTAGCCATTTATTATTTCCTTCCTCTTTTCTACAGTAT TTAAAGATACCCCAAGAAGCTAATTATAACAAGACGAACTCCAATTCACTGTTCCTTGCA TTCTAAAACCTTAAATACCAGAAAACAGCTTTTTCAAAGTTGTTTTCAAAGTTGGCGTAT AACATAGTATCGACGGAGCCGATTTTGAAACCACAATTATGGGTGATGCTGCCAACTTAC TGATTTAGTGTATGATGGTGTTTTTGAGGTGCTCCAGTGGCTTCTGTTTCTATCAGCTGT CCCTCCTGTTCAGCTACTGACGGGGTGGTGCGTAACGGCAAAAGCACCGCCGGACATCAG CGCTATCTCTGCTCTCACTGCCGTAAAACATGGCAAC
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pRI101-AN質粒載體應用舉例
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