| 質粒名稱: | DR274 ，pDR274 |
|---|---|
| 出品公司: | 愛迪基因 |
| 目錄編號: | 42250 |
| 存儲實驗室: | Keith Joung |
|
載體骨架基本信息 |
|
| 載體名稱: | Zebrafish-gRNA-ExpressionVector |
| 載體出品公司: | Joung lab DR274 |
| 載體抗性: | 卡那霉素 |
| 空載體大小: | -- |
| 完整質粒大小: | 2147 bp |
| 載體修飾: | -- |
| 載體類型: | 蠕蟲表達式 |
| 篩選標記: | 新霉素（G418） |
| 高拷貝/低拷貝: | 高拷貝 |
| 生長條件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名稱: | -- |
| 物種來源: | -- |
| 插入大小: | -- |
| 基因突變: | -- |
| GenBank編號 | -- |
| 啟動子 | -- |
| 標簽/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位點: | -- |
| 3' 克隆位點: | -- |
| 5' 測序引物 | -- |
| 3' 測序引物 | -- |
|
文獻引用方法 |
|
| 材料和方法部分: | DR274 was a gift from Keith Joung (Addgene plasmid # 42250 ; //n2t.net/addgene:42250 ; RRID:Addgene_42250) |
| 參考文獻部分: | Efficient genome editing in zebrafish using a CRISPR-Cas system. Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT, Yeh JR, Joung JK. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501. 10.1038/nbt.2501 PubMed 23360964 |
買家導航
DR274載體 pDR274質粒基本信息
DR274質粒圖譜 pDR274載體圖譜和DR274載體序列 pDR274質粒序列多克隆位點信息
DR274質粒 pDR274載體簡介
DR274質粒序列 pDR274載體序列
LOCUS DR274 2147 bp ds-DNA circular SYN 23-AUG-2019
DEFINITION guide RNA (gRNA) expression vector used to create a gRNA to a
specific sequence; uses T7 promoter for in vitro transcription.
ACCESSION .
VERSION .
KEYWORDS DR274
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2147)
AUTHORS Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT,
Yeh JR, Joung JK
TITLE Efficient genome editing in zebrafish using a CRISPR-Cas system.
JOURNAL Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501.
PUBMED 23360964
REFERENCE 2 (bases 1 to 2147)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 23, 2019 from SnapGene Server 1.1.58
//www.snapgene.com
FEATURES Location/Qualifiers
source 1..2147
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 9..26
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 10..26
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_RNA complement(208..283)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter complement(305..323)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(452..468)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(452..468)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
terminator 516..547
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
promoter 548..650
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 651..1466
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(724..743)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
terminator 1490..1517
/label=T7Te terminator
/note="phage T7 early transcription terminator"
rep_origin complement(1529..2116)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1609..1628)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cccacccgtg taaaacgacg gccagtttat ctagtcagct tgattctagc tgatcgtgga
61 ccggaaggtg agccagtgag ttgattgcag tccagttacg ctggagtctg aggctcgtcc
121 tgaatgatat gcgaccgccg gagggttgcg tttgagacgg gcgacagatc cagtcgcgct
181 gctctcgtcg atccaagctt tttaaaagca ccgactcggt gccacttttt caagttgata
241 acggactagc cttattttaa cttgctattt ctagctctaa aactgagacc ctctctcggt
301 ctctcctata gtgagtcgta ttagctagcg gtgcgagcgg atcgagcagt gtcgatcact
361 actggaccgc gagctgtgct gcgacccgtg atcttacggc attatacgta tgatcggtcc
421 acgatcagct agattatcta gtcagcttga tgtcatagct gtttcctgag gctcaatact
481 gaccatttaa atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct
541 tttgacttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac
601 cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata
661 ttcaacggga aacgtcttgc ttgaagccgc gattaaattc caacatggat gctgatttat
721 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt
781 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
841 atgttacaga tgagatggtc aggctaaact ggctgacgga atttatgcct cttccgacca
901 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atcccaggga
961 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
1021 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacggcg
1081 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttggtgcga
1141 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
1201 aactcttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
1261 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
1321 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
1381 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
1441 acttgatgct cgatgagttt ttctaatgag gacctaaatg taatcacctg gctcaccttc
1501 gggtgggcct ttctgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
1561 acaaaaatcg atgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
1621 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
1681 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
1741 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
1801 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
1861 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
1921 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
1981 gtatctgcgc tctgctgaag ccagttacct cggaaaaaga gttggtagct cttgatccgg
2041 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
2101 aaaaaaagga tctcaagaag atcctttgat tttctaccga agaaagg
//
DR274載體圖譜 pDR274質粒圖譜pdf版和DR274載體序列 pDR274質粒序列等相關資料下載
DR274質粒 pDR274載體應用舉例
- 上一篇：pGL3 BRE Luciferase
- 下一篇：p14-9VNA5