| 質粒名稱: | pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 89609 |
| 存儲實驗室: | Vladislav Verkhusha |
|
載體骨架基本信息 |
|
| 載體名稱: | CCLc |
| 載體出品公司: | Luigi Naldini, PMID 9765382 |
| 空載體大小: | 8499bp |
| 完整質粒大小: | 9070bp |
| 載體類型: | 哺乳細胞表達, Lentiviral |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷貝數: | 未知 |
|
插入基因信息 |
|
| 插入基因名稱: | VEGF-A(165) |
| 物種來源: | H. sapiens (human) |
| 插入大小: | 423bp |
| 基因突變: | K188E |
| GenBank編號 | NM_001025366.1 |
| 啟動子: | MNDU3 |
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | EcoRI (not destroyed) |
| 3’克隆位點: | EcoRI (not destroyed) |
|
文獻引用方法 |
|
| 材料和方法部分: | pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE was a gift from Fernando Fierro (Addgene plasmid # 89609 ; //n2t.net/addgene:89609 ; RRID:Addgene_89609) |
買家導航
pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE載體質粒基本信息
pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE質粒圖譜載體圖譜和pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE載體序列質粒序列多克隆位點信息
pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE質粒載體簡介
pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE質粒序列載體序列
LOCUS pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE 9070 bp DNA circular SYN 14-APR-2025
DEFINITION over-expression of VEGF-A(165) in human cells.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9070)
TITLE Fernando Fierro lab plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9070)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT Sequence Label: pCCLc-MNDU3-VEGFA-PGK-EGFP-WPRE
FEATURES Location/Qualifiers
source 1..9070
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 36..57
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(72..77,78..95,96..102)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 110..126
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 115..137
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 134..150
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 134..150
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 169..189
/label=T3
/note="T3 promoter, forward primer"
promoter 171..189
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
enhancer 290..669
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 670..873
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 824..844
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 888..1068
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1115..1240
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1733..1966
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2151..2195
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2492..2609
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 3837..4336
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(3872..3895)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 4269..4288
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
regulatory 4357..4366
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS join(4363..4365,4366..4368,4369..5082)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS join(4363..4365,4366..4368,4369..5079)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4408..4429)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4669..4688)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5016..5037
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 5125..5713
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5178..5198)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5596..5607)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5805..6038
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6110..6231
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6147..6166)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6201..6220
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6271..6406
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(6426..6445)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6427..6445)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6455..6472)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6455..6471)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6464..6486)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6613..7068
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6700..6719)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6910..6931
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 7094..7198
/gene="bla"
/label=AmpR promoter
CDS join(7199..7267,7268..8059)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7417..7436)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8230..8818
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8719..8738
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8972..8989
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
61 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
121 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
181 cactaaaggg aacaaaagct ggagctgcaa gcttggccat tgcatacgtt gtatccatat
241 cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta
301 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
361 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
421 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
481 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
541 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
601 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
661 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
721 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
781 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
841 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgggg tctctctggt
901 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
961 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
1021 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
1081 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
1141 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
1201 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
1261 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
1321 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
1381 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
1441 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
1501 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
1561 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
1621 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
1681 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
1741 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
1801 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
1861 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
1921 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
1981 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
2041 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
2101 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
2161 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
2221 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
2281 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
2341 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
2401 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgataag
2461 ctaattcaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
2521 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
2581 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
2641 tttgggaatt agcttgatcg attagtccaa tttgttaaag acaggatatc agtggtccag
2701 gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata
2761 gaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
2821 tttggcaagc taggatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga
2881 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagttgga acagcagaat
2941 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
3001 atggtcccca gatgcggtcc cgccctcagc agtttctaga gaaccatcag atgtttccag
3061 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
3121 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
3181 ctcggcgcga tctagatctc gaatcgaatt cgcccttcct gagatcaccg gtaggagggc
3241 catcatgaac tttctgctgt cttgggtgca ttggagcctt gccttgctgc tctacctcca
3301 ccatgccaag tggtcccagg ctgcacccat ggcagaagga ggagggcaga atcatcacga
3361 agtggtgaag ttcatggatg tctatcagcg cagctactgc catccaatcg agaccctggt
3421 ggacatcttc caggagtacc ctgatgagat cgagtacatc ttcaagccat cctgtgtgcc
3481 cctgatgcga tgcgggggct gctgcaatga cgagggcctg gagtgtgtgc ccactgagga
3541 gtccaacatc accatgcaga ttatgcggat caaacctcac caaggccagc acataggaga
3601 gatgagcttc ctacagcaca acaaatgtga atgcagacca aagaaagata gagcaagaca
3661 agaaaatccc tgtgggcctt gctcagagcg gagaaagcat ttgtttgtac aagatccgca
3721 gacgtgtaaa tgttcctgca aaaacacaga ctcgcgttgc aaggcgaggc agcttgagtt
3781 aaacgaacgt acttgcagat gtgacgagcc gaggcggtga aagggcgaat tctaccgggt
3841 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
3901 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
3961 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
4021 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
4081 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
4141 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
4201 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
4261 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
4321 atctccgggc ctttcgacca tctagatcca ccggtcgcca ccatggtgag caagggcgag
4381 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
4441 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
4501 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
4561 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
4621 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
4681 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
4741 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
4801 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
4861 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
4921 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
4981 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
5041 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggcca actcgaggga
5101 tcccccgggg tcgactgatc cgataatcaa cctctggatt acaaaatttg tgaaagattg
5161 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
5221 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
5281 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
5341 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
5401 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
5461 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
5521 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
5581 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
5641 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
5701 gccgcctccc cgcatcggat caaattcgag ctcggtacct ttaagaccaa tgacttacaa
5761 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
5821 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat
5881 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
5941 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
6001 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
6061 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat
6121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
6181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
6241 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
6301 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
6361 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca
6421 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg
6481 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
6541 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
6601 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
6661 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
6721 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
6781 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
6841 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
6901 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
6961 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
7021 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
7081 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
7141 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
7201 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
7261 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
7321 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
7381 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
7441 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
7501 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
7561 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
7621 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
7681 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
7741 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
7801 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
7861 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
7921 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
7981 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
8041 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
8101 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
8161 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
8221 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
8281 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
8341 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
8401 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
8461 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
8521 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
8581 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
8641 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
8701 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
8761 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
8821 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
8881 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
8941 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
9001 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
9061 cgacaggttt
//