LOCUS       pGR475       11561 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin 
            resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11561)
  TITLE     Continuous evolution of user-defined genes at 1-million-times the 
            genomic mutation rate
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            //gt12365.cn
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGR475
FEATURES             Location/Qualifiers
     source          1..11561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      108..696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     597..616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     850..867
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    984..1005
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1020..1025,1026..1043,1044..1050)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1058..1074
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1063..1085
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1082..1098
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1082..1098
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(1658..2686)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             complement(1658..2686)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     primer_bind     10439..10458
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10533..10637
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10638..10706,10707..11498)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGEPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10856..10875)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
       61 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
      121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
      181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
      241 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
      301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
      361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      481 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      721 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      781 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      841 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      901 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      961 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     1021 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     1081 acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga cgggaattct
     1141 aagctctttg ttttctaatt tagaatactc acctaactca gacattatcg gaacatgtat
     1201 atctgttaga ttctggtcga gatattcttt aatggtttag tgtatgttgc gcttactact
     1261 ttttggcgtt tttgcctatt tcactattta catatcgtga aaatgacatt tggttctagg
     1321 ttcgggtgac gtgaagataa cgaaaaatga gtaaaaatta tcttctaatt atacattagt
     1381 attagcgtga aaatgtacac atatacatat atatatatat atatatatac ttgaaggtct
     1441 gaaggagttt caaatgcttc tactccgtct gctttctttt cgggagcaag attgttgtgg
     1501 tgaatcatcg ataaaaataa atatactgag attatagtaa gctcattgat cccttaaact
     1561 ttcttttcgg tgtatgactt atgagggtga gaatgcgaaa tggcgtggaa atgtgatcaa
     1621 aggtaataaa acgtcatata ttaccctgtt atccctatta ttcctttgcc ctcggacgag
     1681 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg
     1741 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg
     1801 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat
     1861 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct
     1921 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc
     1981 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa
     2041 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca
     2101 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg
     2161 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag
     2221 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg
     2281 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga
     2341 ccggttgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg
     2401 cacggcggga gatgcaatag gtcaggctct cgctaaattc cccaatgtca agcacttccg
     2461 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat
     2521 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac
     2581 gagattcttc gccctccgag agctgcatca ggtcggacac gctgtcgaac ttttcgatca
     2641 gaaacttctc gacagacgtc gcggtgagtt caggcttttt acccattttg ttttttgttt
     2701 tcttctaatt gattttttct ttctatttcc tttggtgaaa caggtagttt tgttcaaaag
     2761 aaatcattca atggtttaaa taaaagcaag aagtatgaaa atttttttat tttttcttca
     2821 gaattttatc atagttgtcg taaaataacg aggtcgggta acgtgtttgg tgtactgagg
     2881 tcttttttaa aagaagctcc agcaagaaag agttttgtgt gatggctaat gaagtgtcaa
     2941 ttgcatttgg aaagcagcct gatttcttgg gcgtgaatat gaaattcttc ttttgttatt
     3001 ttataatatg aagtatgaat gaaaacataa agcgcgctga gacaacgact gttcaatgta
     3061 aaataaagaa aatctatcgt tctcaagacg tggtcccatt tttttttact ttgaatttcg
     3121 tcagcagtgt gacatggtta gaatgtaacc ccacatttgc aaagagattg ttacaggagc
     3181 gttctaattg tcccgtactg gtacattcat aagtgaaaag aaagccatat cgggcgcgct
     3241 ggtttgtgag ccacagaaac cggacaaggc acttcttgaa tacttcccaa ggaaagcgcc
     3301 aaggtttacg gttctgatgg gaatgcaggg gggaatctag tcttgaagta tccttattcc
     3361 ttaaaaaaaa tattggacat cctcttgtgt tgttatccga tacaaccgga tatttttctt
     3421 ttaatgagtc taaaccgtga tagcttcagg ttaatacaat caaaaaaagc tcaaatattc
     3481 ttttaatgcc gcgttcacag attccaattg aatacaacta ggtagttcat tatatgaagc
     3541 ctttgctact atttttcact atagtctgcc ttcaccttaa tgcagacatc cacatatttt
     3601 aatcacttta aaataaaaag gaagatatat tagaagctat gatccaatct gtaagccaga
     3661 ttaaaattca cgaactcttc tttcatttga attgaatgct ttgagttggg gtagattatc
     3721 gcaaattact catcacattt attgactacg aacttgctga tgtccttttt ttatttatat
     3781 ttttcttcag tgaagcgatt ttttttttac acagaccaag acggaaaaaa gtagctaagg
     3841 aagaaaacaa aatcatgaaa aaaatgtgaa gtgatcatgc acatcgcatc aacttaaaca
     3901 ttggcttaga gatatataga gttagagttt acggcaacct ttaagcacca ataccttttg
     3961 gcatagtcta aagacctggt tcttaatttt aaacaaattt aactaaagat ttccctatca
     4021 aagaagtaac gagttgacag attttctcaa aataaatcga tactgcattt ctaggcatat
     4081 ccagcgagat ctatggatta caaggacaag gcattgaacg atttgagaaa cgtttatgca
     4141 gacttcgact ctttaccatt agatttcaga caaatcttga ttaaagatag agcaactttg
     4201 ttacaaaaag aagatgttga aaagaagatt ttagaaagac aggaagatgc taagaagtac
     4261 gcagaatatt tgaaacaatc tgaaatccct gaaagaattt ctttaccaaa cattaaaaga
     4321 cataagggtg tttcaatctc ttttgaagaa acttcagaag atatggtttt agaaccacgt
     4381 ccttttattt ttgatggttt gaatatcaga tgttttagaa gggaaaccat cttctcattg
     4441 aagaataaga tcctaaatat ggttaaggaa tcatcttctt tcaagaatgt ttctagacaa
     4501 tcagtttctt tcatgtactt caagattttc aataaaggta aggttattgc ttcaactaaa
     4561 tcagttaata tctacgaaga taaaattgat gaaagattag aagacttgtg taataatttt
     4621 gatgatgttt tgaagaaaat tattgatgtt acttacggtt acgaatcttt atttgtttca
     4681 gaaacatact cttatgttat tttctacgct aagtctatct atttcccaca accaagatgt
     4741 gttaataatt ggggtaataa tattccaaac attttgactt tcgattcttt taaattgttt
     4801 actgcaaata agaataatgt ttcatgtatt aaacaatgtt ctagattctt gtggcaaaag
     4861 gattttaata cattggaaga aatgatcgaa tacaaaaacg gtaacatttg tattgttact
     4921 ccacaattac atattaatga tgttagagac attaaatctt ttaatgatat cagattatac
     4981 tctgaatccc caattaagac tttctctgtt attgacaaca caatcactta cttgttttat
     5041 ttcaaagaac atttaggtgt tattttcaat attactaaat ctagacatga taggagagtt
     5101 acaaagtttt ctccattgtc taaattttca gatgttaaaa atattacagt ttgttttgat
     5161 attgaatcat atttcgatcc agaaaaagaa tcaaatcaag ttaatattcc ttttatttgt
     5221 tgtgcttcta ttatctataa caaagttatc ggtaatattg ttgatttcga aggtagagat
     5281 tgtgttgctc aaatgattga atatgttgtt gatatttgtg gtgaattaaa catttcatct
     5341 gttgaattaa ttgctcataa tggtggtggt tacgatttcc attatatctt atcttctatg
     5401 tataacccag cagctattaa aaatatctta atcagaaata attctttcat ttcatttaac
     5461 tttgctcatg atggtgttaa attttctgtt aaagattcat actctttttt gttatgttca
     5521 ttggctaatg catctaaagc ttttttgaat gaagaaacat ttaaaaaaac agattttcca
     5581 catcacgatt tgaagactgc tgatgatttg tacaaggttt acaaagaatg gtcatctgtt
     5641 aatactgaaa ttaatcatgt tgttgaaaaa gaaaaattgt taattacttc tgaacatatc
     5701 gttaatttta ctaaaaatga taaatcaaaa acattaattg aatggtctaa agattattgt
     5761 agaaatgatg ttttggtttt atcaaaagtt tggttagagt tcaagaacgc tgttgaagat
     5821 attttcaatt gtgaattagt tgatcaaact atgacattgg caggtttgtc ttataaattg
     5881 tttcaagcta atatgccatt tgatgttgaa ttaagacatc caaataaaga agattacttt
     5941 aatatgagag aagctttaat cggtggtagg tgtatttcag ttaatggtat ttataaagat
     6001 gttttgtgtt tagatgttaa atctttatac ccagcttcaa tggcattcta cgatcaacca
     6061 tacggttcat ttaaaagagt ttctagcaga ccaaaggatg aattaggtat ctactatgtt
     6121 agagttacac caaatagaaa taataagtca aatttctttc caatcagatc tcataacaaa
     6181 atcacataca ataactttga agaatctact tacatcgctt ggtatactaa tgttgatatt
     6241 gatatcggtt tgtctgaagg tcataacatt gaatatatcc cattcgattc ttacggtaat
     6301 atcggttatt catggtctaa gaaaggtaag atcttcgaaa agtacatcaa agatgttttg
     6361 tacaagttga agataaagta tgaaaaacaa aataataagg ttaaaagaaa tgttattaaa
     6421 attattatga actctttgtg gggtaaattt gctcaaaaat gggttaactt cgaatatttt
     6481 atcaaatctg aagatgatat cgattttgaa tctgaagaag cttacaagat ctgggatact
     6541 gatttcatgt taattaagaa aattaaagaa tcaacatact catctaaacc aatccaaaat
     6601 ggtgttttta cattatcatg ggctagatac cacatgaaat caatttggga tgctggtgca
     6661 aaggaaggtg cagaatgtat atattctgat acagattcta tcttcgttca caaagaacat
     6721 ttcaagaaaa atgctaaatt tatgttgaat ggtttgaagg ttccaattat tggttcagaa
     6781 gttggtcaat tagaattgga atgtgaattt gataagttat tgtgtgcagg taagaaacaa
     6841 tacatgggtt tctatacata ttttcaagat ggtaagccat gtattaaaga aaaaaagaga
     6901 tttaagggta ttccatcaaa ttatatcatc ccagaattgt acgctcattt gttgtctggt
     6961 gctgataaag aagcaaagat ccaattcttg aagtttagaa gggaatgggg ttctgttaaa
     7021 ggttatattg aaaacaaaac tgttaaggca acataaattt ttcaaactgc aaattcaaga
     7081 aaaagccacg cgtgtgcacc ttttttttcc ccttccagtg cattatgcaa tagacagcac
     7141 gagtctttga aaaagtaact tataaaactg tatcaatttt taaacctaaa tagattcata
     7201 aactattcgt taatataaag tgttctaaac tatgatgaaa aaataagcag aaaagactaa
     7261 taattcttag ttaaaagcac tttatgctac aacattccaa aatttgtccc aaaaagtctt
     7321 tggttcatga tcttcccata caattgcctc aatgtctctt ctatcggaat cgatgtcgac
     7381 atctccaatc ttccaaataa atctgcatct gaatatgcat tgaaataaga tccaaacagc
     7441 taagaacagg aaaatagaga tataggcggc agcaaagcta acaccattga attttggtgc
     7501 aaaagccgtg aaaccttgaa taatgataat gatcgtcata aatgtggccg cataataagc
     7561 caagccgggc attaatttag ctttaaatgg taactcgtca cgagagatgc cacggtattt
     7621 caaagcttgc ataaatctga tgtgcgagat tgagataaat aaccatgcaa aaaagcctgc
     7681 aacaccagtg atatttaata gccattcgaa aactttgtca ccaccagtag atgtctccat
     7741 gtaagccaaa gcgccaaatg cagcagtaac gaaaactgca atgtatggaa caccaccttt
     7801 ggtggtcctt gacaggaatt taggagccaa cttgttcttt gatagaccaa ataaaatacg
     7861 ggaaccaacg taaatatttg aatttgcggc agaaataatg gttgttaaga taacagcgtt
     7921 gaagatatgt ggcaaaacct ttgtaccaga gttctcaata gcaataataa agggagaagt
     7981 agaaacgtag gaagtagatt gtgttagttt agggtcattg tatggaacta aaagtccaat
     8041 gaataataga gagccaatgt agaaggttaa gatacggaaa acaacttttt tgatggctct
     8101 tggaacggat tttctggggt ttgcagcttc accagcagtg ataccaacta gttcagtacc
     8161 ttgaaatgtg aaggcagcgt taatcaaaga ggaaacccaa cctaagaacc tcccttcgtt
     8221 tttatcctta gatattatac ctggacccca ggcacctggg tttctccaat aacggaatcc
     8281 aactgggccg gtaaccccag caccacaaac catacaaaaa cagtatatta gaaacccgat
     8341 aatggctaaa actttgatgg aagcgaccca gaactcaaat tcaccgtaat atttgacagg
     8401 gaacaagttc attattgtga taattaccca aaaaatacta atccatgccg ccagtggaac
     8461 tttgtacgtc caaaattgaa tgacttggcc aactacacta agttccaggg caaaagtgat
     8521 tgcccaagaa aaccaataca tgtaaccatt ggccgcacca aatgctggag aaaggaatct
     8581 ttgtgagaaa actgtgaaag aggatgtaac agggatgaat gtagccattt cacccaagga
     8641 ctgcgtgaca gaatatgcca aagaacccat aaataaatat gatataagag cgcccactgg
     8701 gccggcgttg gtcagaggtg tggataaacc aatgaaaaga cctgtaccaa tagtaccacc
     8761 aagggcaatc ataccaatat gtctttgctt aagctctctc ttcacttcag cgttctgtac
     8821 ttctccttca tcttcatcac ctatgccatc ctccatagag aacgtatcct cgccatttac
     8881 tctcgtcggg aaagagcgca atggatacaa ttctttactt ttctcatctt tcaatggtat
     8941 tgacccacgt ctgtggtgtg tttgtgaagc ttcaacgtcg tgaaagaggg ttgtgaccgg
     9001 ctcattgtac atatgcttct cctctatgtc ggcgtcttct tttgaatttg tcatttcttc
     9061 ccgttccagc actctatgtg attaagatcg agtgagcaaa cgtgtttttc taggatgatt
     9121 tcttgaccaa ttctttgtgg agataagctt taaagtctga agaacgtatc tctgtagttc
     9181 ttctgtctca tttaaaataa cccaaaattt ccgaaatttc agtgcagctc aaccctaaat
     9241 tagacctaat tgaaatcagt agggttagct cgtatttgaa atattacaaa acaagtccta
     9301 taaaactcta atcttaaccc ttatgttaga ttttcagcac gtgattttgc atctttttta
     9361 gttttgctta aaaaattttg acctctacct tttctctctc tgtttaatca ggtaataaaa
     9421 agttcagcca aaaagtgtgg ttgtgaaatt ttattctggc gatgcgcaat cggagtcttt
     9481 tctatttctt gcaactcgca gaagaaaagg tattttacta cataaattgt atatattcct
     9541 tctcaggatc agcaacataa gatattggct ttgatattat attgcatctt attgcattgt
     9601 gtgttgtggt taagacaaat aaataaagga agtttcttct tttttttcct tccttttttt
     9661 cctctctcag ataaaagttc aacagagcca tggtgtcgaa ggatcaaacg tcctttaata
     9721 agaggtggac cttgggtcat ctgatgttgg gtcatgctat gccttttttt ttttttgttt
     9781 ttacaggagt taagaagtct gaagaactct gaaataaact ttcgattgac gacagattaa
     9841 aaactggatc caactataag tataatagta acttatatat ttctgttcca gatatatacg
     9901 taaaaaaaag aaaaggataa ttgatgtttt tgcaaaaagg gattccctga ttcggtttac
     9961 tctgttcgca accactcttc tttctgctaa tttgtgcttt atctcgtatc attctcttat
    10021 ctacttatct taaccttatc gtcttcctcg aaattttcat tgatagagac aactgcaaaa
    10081 aaagaaattc aaaaaagccg tatgactcac ccggaaacca cacagaaacc ctagatttct
    10141 atagggcaaa tttcagggtt atactaaaag atatattatt gaccttggtt attcttcaag
    10201 ttcgccctat tgaaaattcg gacactttag ggtttggaat ctgttaaaga tatagaattc
    10261 actcaccaac gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg cctaacccta
    10321 agcagattct tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta aattttgctc
    10381 gcgcactact cagcgacctc caacacacaa gcagggagca gatactggct taactatgcg
    10441 gcatcagagc agattgtact gagagtgcac cataggggat cgggagatct cccgatccgt
    10501 cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    10561 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10621 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10681 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10741 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10801 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10861 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    10921 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    10981 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    11041 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    11101 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    11161 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    11221 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    11281 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    11341 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt gagccctccc
    11401 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    11461 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    11521 atatacttta gattgattta aaacttcatt tttaatttaa a
//