| 質粒名稱: | 19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 209264 |
| 存儲實驗室: | Wensheng Wei |
|
載體骨架基本信息 |
|
| 載體名稱: | pCMV |
| 載體出品公司: | Clontech |
| 完整質粒大小: | 6616bp |
| 載體類型: | 哺乳細胞表達 |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | SOD2-3xHA-TALE(RNR2-Left)-MutH |
| 物種來源: | H. sapiens (human) |
|
文獻引用方法 |
|
| 材料和方法部分: | 19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH was a gift from Wensheng Wei (Addgene plasmid # 209294 ; //n2t.net/addgene:209294 ; RRID:Addgene_209294) |
| 參考文獻部分: | Strand-selective base editing of human mitochondrial DNA using mitoBEs. Yi Z, Zhang X, Tang W, Yu Y, Wei X, Zhang X, Wei W. Nat Biotechnol. 2023 May 22. doi: 10.1038/s41587-023-01791-y. 10.1038/s41587-023-01791-y PubMed 37217751 |
買家導航
19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH載體質粒基本信息
19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH質粒圖譜載體圖譜和 19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH載體序列質粒序列多克隆位點信息
-MutH質粒圖譜.png)
19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH質粒載體簡介
19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH質粒序列載體序列
LOCUS 19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH 6616 bp DNA circular SYN 14-APR-2025
DEFINITION mitoBE expression vector, target mtRNR2 gene..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6616)
AUTHORS Yi Z, Zhang X, Tang W, Yu Y, Wei X, Zhang X, Wei W
TITLE Strand-selective base editing of human mitochondrial DNA using
mitoBEs.
JOURNAL Nat Biotechnol. 2023 May 22. doi: 10.1038/s41587-023-01791-y.
PUBMED 37217751
REFERENCE 2 (bases 1 to 6616)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Biotechnol. 2023 May 22. doi: 10.1038/s41587-023-01791-y."
COMMENT Sequence Label: 19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH
FEATURES Location/Qualifiers
source 1..6616
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 132..335
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 286..306
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 377..396
/label=T7
/note="T7 promoter, forward primer"
promoter 377..395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 421..430
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 520..546
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 550..576
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 580..606
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(3972..3988)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3972..3988)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3985..4007)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3996..4012
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(4020..4026,4027..4044,4045..4050))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4065..4086
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4203..4220)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4374..4962)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4454..4473)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(5133..5924,5925..5993))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5756..5775
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5994..6098)
/gene="bla"
/label=AmpR promoter
primer_bind complement(6177..6196)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer join(6368..6616,1..131)
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagac ccaagctggc tagcaccgcc
421 gccaccatgg ccctgtcccg tgcggtttgt ggcacctccc gtcaactggc tccggttctg
481 ggttatctgg gttcccgtca aaaacactcc ctgccggact acccgtatga tgttccggat
541 tacgctggct acccatacga cgtcccagac tacgctggct acccatacga cgtcccagac
601 tacgctatgg acatcgcgga cctcaggacg ctgggctaca gccagcaaca gcaagagaag
661 atcaagccca aagtaaggag caccgtggcc cagcaccacg aggccctggt gggtcacggc
721 ttcacccacg cgcatatcgt tgctctgagc caacatcccg cagctctggg taccgttgcg
781 gtgaagtatc aggacatgat cgcggcactg cctgaagcta cacacgaagc catagtgggc
841 gttggcaagc agtggagcgg tgccagagcg cttgaggcac tgttgacggt ggctggcgag
901 ctgaggggac cgccactgca actggacacc ggccaactgc tgaagatcgc caagagggga
961 ggcgtgacgg cggtggaggc cgtgcatgcc tggaggaacg cactgaccgg cgcgcccctg
1021 aacctgacac cagagcaagt agtggctatt gcaagtcacg acggtggcaa acaagcgctg
1081 gagaccgtgc agaggctcct tccggtgctc tgccaagcac acggtctcac tccggaacag
1141 gtggtcgcaa tcgcgagcaa catcggcggc aagcaagccc ttgagacagt ccaaagactt
1201 ttgcctgtcc tttgtcaggc gcatggcctt acgcctgagc aagtcgttgc gatcgcctcc
1261 aacatcggcg gaaaacaggc tttggaaacc gtgcagcggt tgctgcccgt tttgtgccaa
1321 gcccacggat tgacccccga acaggttgta gccatagctt ctcacgacgg aggtaagcag
1381 gcactggaaa ccgtgcagcg cctgctccca gtactgtgtc aggctcatgg gctgacacca
1441 gagcaagtag tggctattgc aagtaacatc ggtggcaaac aagcgctgga gaccgtgcag
1501 aggctccttc cggtgctctg ccaagcacac ggtctcactc cggaacaggt ggtcgcaatc
1561 gcgagcaaca tcggcggcaa gcaagccctt gagacagtcc aaagactttt gcctgtcctt
1621 tgtcaggcgc atggccttac gcctgagcaa gtcgttgcga tcgcctccaa cggcggcgga
1681 aaacaggctt tggaaaccgt gcagcggttg ctgcccgttt tgtgccaagc ccacggattg
1741 acccccgaac aggttgtagc catagcttct aacatcggag gtaagcaggc actggaaacc
1801 gtgcagcgcc tgctcccagt actgtgtcag gctcatgggc tcactccgga acaggtggtc
1861 gcaatcgcga gcaacaacgg cggcaagcaa gcccttgaga cagtccaaag acttttgcct
1921 gtcctttgtc aggcgcatgg ccttacgcct gagcaagtcg ttgcgatcgc ctccaacaac
1981 ggcggaaaac aggctttgga aaccgtgcag cggttgctgc ccgttttgtg ccaagcccac
2041 ggattgaccc ccgaacaggt tgtagccata gcttctaaca acggaggtaa gcaggcactg
2101 gaaaccgtgc agcgcctgct cccagtactg tgtcaggctc atgggctgac accagagcaa
2161 gtagtggcta ttgcaagtaa cggcggtggc aaacaagcgc tggagaccgt gcagaggctc
2221 cttccggtgc tctgccaagc acacggtctc actccggaac aggtggtcgc aatcgcgagc
2281 aacggcggcg gcaagcaagc ccttgagaca gtccaaagac ttttgcctgt cctttgtcag
2341 gcgcatggcc ttacgcctga gcaagtcgtt gcgatcgcct ccaacggcgg cggaaaacag
2401 gctttggaaa ccgtgcagcg gttgctgccc gttttgtgcc aagcccacgg attgaccccc
2461 gaacaggttg tagccatagc ttctaacatc ggaggtaagc aggcactgga aaccgtgcag
2521 cgcctgctcc cagtactgtg tcaggctcat gggctgacac cagagcaagt agtggctatt
2581 gcaagtcacg acggtggcaa acaagcgctg gagaccgtgc agaggctcct tccggtgctc
2641 tgccaagcac acggtctgac accagagcaa gtagtggcta ttgcaagtaa caacggtggc
2701 aaacaagcgc tggagaccgt gcagaggctc cttccggtgc tctgccaagc acacggtctc
2761 actccggaac aggtggtcgc aatcgcgagc aacatcggcg gcaagcaagc ccttgagagc
2821 atcgtggccc agctgagcag acccgaccct gccctcgccg ctctgaccaa cgaccacctg
2881 gtggccctgg cttgcctcgg gggcaggcca gctcttgacg ccgtgaagaa gggccttggt
2941 ggatccatgt cacaaccaag accgttgttg tcccccccag aaacagagga acagctcctc
3001 gcacaggcac aacagctcag tggttacact cttggagaat tggcggcatt ggttggactc
3061 gttacccccg aaaaccttaa acgcgacaaa ggttggatcg gcgttcttct tgaaatttgg
3121 ttgggcgcaa gcgcaggttc aaaacccgaa caagactttg cggcgttggg cgttgagctg
3181 aagaccattc ctgtagatag cttgggtcga ccattggaga ccacgttcgt ttgcgtcgct
3241 cctctgactg gaaactcagg agtcacatgg gaaacatccc acgtaaggca taagctcaaa
3301 cgagtgcttt ggatcccggt ggaaggtgag agaagcatcc cccttgctca aaggcgagtg
3361 ggatcacccc ttctgtggtc cccaaacgag gaagaggatc gccaactgag ggaggactgg
3421 gaagaattga tggacatgat cgtcctcggg caagtcgaga gaattaccgc gagacacggt
3481 gagtacttgc agattcgccc taaggctgca aatgctaagg ctttgactga ggctattgga
3541 gccagaggcg agcgcattct gactctgcct aggggatttt acctgaagaa gaacttcact
3601 tccgcacttc ttgcgaggca ctttttgata cagtagtgat aaaccacgat cgttatgctg
3661 atcataccct aatgatccca gcaagataat gtcctgtctt ctaagatgtg catcaagcct
3721 ggtacatact gaaaacccta taaggtcctg gataattttt gtttgattat tcattgaaga
3781 aacatttatt ttccaattgt gtgaagtttt tgactgttaa taaaagaatc tgtcaaccat
3841 caaaaccggt gtttaaacga agacaatagc aggcatgctg gggatgcggt gggctctatg
3901 gcttctgagg cggaaagaac cagctggggc tcgataccgt cgacctctag ctagagcttg
3961 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
4021 aacatacgag ccggaagcat aaagtgtaaa gcctagggtg cctaatgagt gagctaactc
4081 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
4141 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
4201 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
4261 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
4321 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
4381 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
4441 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
4501 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
4561 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
4621 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
4681 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
4741 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
4801 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
4861 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
4921 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
4981 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
5041 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
5101 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
5161 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
5221 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
5281 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
5341 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
5401 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
5461 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
5521 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
5581 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
5641 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
5701 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
5761 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
5821 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
5881 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
5941 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
6001 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
6061 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
6121 cctgacgtcg acggatcggg agatcgatct cccgatcccc tagggtcgac tctcagtaca
6181 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
6241 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
6301 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
6361 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
6421 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
6481 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
6541 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
6601 gtacatcaag tgtatc
//