| 質粒名稱: | pcDNA3-AKAR4-NES |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 64727 |
| 存儲實驗室: | Jin Zhang |
|
載體骨架基本信息 |
|
| 載體名稱: | pcDNA3 |
| 載體出品公司: | Invitrogen |
| 空載體大小: | 5446bp |
| 完整質粒大小: | 6594bp |
| 載體類型: | 哺乳細胞表達 |
| 篩選標記: | 新霉素（G418） |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | AKAR4-NES |
| 物種來源: | S. cerevisiae (budding yeast), Synthetic |
| 插入大小: | 2010bp |
| 啟動子: | CMV |
| 標簽/融合蛋白: |
Cerulean (N terminal on insert)
cpVenus[E172] (C terminal on insert)
NES (C terminal on insert)
|
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | BamHI (not destroyed) |
| 3’克隆位點: | EcoRI (not destroyed) |
| 5’測序引物 | T7 forward |
| 3’測序引物 | BGH reverse |
|
文獻引用方法 |
|
| 材料和方法部分: | pcDNA3-AKAR4-NES was a gift from Jin Zhang (Addgene plasmid # 64727 ; //n2t.net/addgene:64727 ; RRID:Addgene_64727) |
| 參考文獻部分: | Spatiotemporally regulated protein kinase A activity is a critical regulator of growth factor-stimulated extracellular signal-regulated kinase signaling in PC12 cells. Herbst KJ, Allen MD, Zhang J. Mol Cell Biol. 2011 Oct;31(19):4063-75. doi: 10.1128/MCB.05459-11. Epub 2011 Aug 1. 10.1128/MCB.05459-11 PubMed 21807900 |
買家導航
pcDNA3-AKAR4-NES載體質粒基本信息
pcDNA3-AKAR4-NES質粒圖譜載體圖譜和pcDNA3-AKAR4-NES載體序列質粒序列多克隆位點信息
pcDNA3-AKAR4-NES質粒載體簡介
pcDNA3-AKAR4-NES質粒序列載體序列
LOCUS pcDNA3-AKAR4-NES 7481 bp DNA circular SYN 14-APR-2025
DEFINITION Cytosol-targeted, FRET-based PKA activity reporter..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7481)
AUTHORS Herbst KJ, Allen MD, Zhang J
TITLE Spatiotemporally regulated protein kinase A activity is a critical
regulator of growth factor-stimulated extracellular signal-regulated
kinase signaling in PC12 cells.
JOURNAL Mol Cell Biol. 2011 Oct;31(19):4063-75. doi: 10.1128/MCB.05459-11.
Epub 2011 Aug 1.
PUBMED 21807900
REFERENCE 2 (bases 1 to 7481)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1128/MCB.05459-11"; journalName: "Mol Cell Biol"; date:
"2011-10"; volume: "31"; issue: "19"; pages: "4063-75"
COMMENT Sequence Label: pcDNA3-AKAR4-NES
FEATURES Location/Qualifiers
source 1..7481
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 901..910
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 922..939
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 943..975
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/label=T7 tag (gene 10 leader)
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS join(979..987,988..1002)
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/label=Xpress(TM) tag
/translation="DLYDDDDK"
CDS join(1008..1010,1011..1013,1014..1724)
/codon_start=1
/product="enhanced monomeric variant of CFP (Rizzo et al.,
2004)"
/label=mCerulean
/note=" mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 2247..2981
/codon_start=1
/product="circularly permuted form of the Venus yellow
fluorescent protein (Nagai et al., 2004)"
/label=cp173Venus
/note="efficient FRET acceptor for CFP"
/translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYITADKQKNGIKANFKIRHNIE"
CDS 2988..3014
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
promoter complement(3033..3051)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(3034..3051)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(3071..3088)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 3077..3301
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3347..3775
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3434..3453)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3644..3665
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3784..3804)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3789..4119
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3970..4105
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4032..4051
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4186..4980
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4240..4259)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4850..4869
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 5154..5275
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5191..5210)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5245..5264
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(5324..5340)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5324..5340)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5337..5359)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5348..5364
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(5372..5378,5379..5396,5397..5402))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5417..5438
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5555..5572)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5726..6314)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5806..5825)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(6485..7276,7277..7345))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7108..7127
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7346..7450)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
901 gccaccatgg tgcggggttc tcatcatcat catcatcatg gtatggctag catgactggt
961 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatccatg gtgagcaagg
1021 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
1081 gccacaggtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
1141 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
1201 tgacctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
1261 tcaagtccgc catgcccgaa ggctacgtcc aggagcgtac catcttcttc aaggacgacg
1321 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
1381 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
1441 acgccatcag tgacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggccc
1501 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
1561 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
1621 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
1681 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagcgcatg cataagtttt
1741 ctcaagaaca gatcggcgaa aacattgtgt gcagggtcat ttgtaccacg ggtcaaattc
1801 ccatccgaga tttgtcagct gatatttcac aagtgcttaa ggaaaaacga tccataaaga
1861 aagtttggac atttggtaga aacccagcct gtgactatca tttaggaaac atttcaagac
1921 tgtcaaataa gcatttccaa atactactag gagaagacgg taacctttta ttgaatgaca
1981 tttccactaa tgggacctgg ttaaatgggc aaaaagtcga gaagaacagc aatcagttac
2041 tgtctcaagg tgatgaaata accgttggtg taggcgtgga atcagatatt ttatctctgg
2101 tcattttcat aaacgacaaa tttaagcagt gcctcgagca gaacaaagtt gatcgctctg
2161 caggtaagcc aggcagcggc gagggcagca ccaagggcct gcgtcgcgcc accctggtgg
2221 acggcggcac cggcggcagc gagctcatgg gcggcgtgca gctcgccgac cactaccagc
2281 agaacacccc catcggcgac ggccccgtgc tgctgccnga caaccactac ctgagctacc
2341 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
2401 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggaggt accggtggat
2461 ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
2521 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
2581 acggcaagct gaccctgaag ctgatctgca ccaccggcaa gctgcccgtg ccctggccca
2641 ccctcgtgac caccctgggc tacggccttc agtgcttcgc ccgctacccc gaccacatga
2701 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
2761 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
2821 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
2881 acaagctgga gtacaactac aacagccaca acgtctatat caccgccgac aagcagaaga
2941 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggaattcctg ccccccctgg
3001 agcgcctgac cctgtaatct agagggccct attctatagt gtcacctaaa tgctagagct
3061 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
3121 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
3181 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
3241 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
3301 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
3361 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
3421 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
3481 ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc tttacggcac
3541 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
3601 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
3661 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgggg
3721 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
3781 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt
3841 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
3901 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
3961 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
4021 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
4081 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
4141 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
4201 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
4261 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
4321 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
4381 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
4441 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
4501 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
4561 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
4621 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
4681 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
4741 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
4801 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
4861 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
4921 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
4981 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
5041 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
5101 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
5161 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
5221 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
5281 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
5341 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
5401 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
5461 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
5521 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
5581 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
5641 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
5701 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
5761 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
5821 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
5881 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
5941 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
6001 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
6061 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
6121 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
6181 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
6241 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
6301 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
6361 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
6421 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
6481 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
6541 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
6601 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
6661 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
6721 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
6781 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
6841 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
6901 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
6961 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
7021 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
7081 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
7141 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
7201 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
7261 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
7321 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
7381 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
7441 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
pcDNA3-AKAR4-NES載體圖譜質粒圖譜pdf版和pcDNA3-AKAR4-NES載體序列質粒序列等相關資料下載
pcDNA3-AKAR4-NES質粒載體應用舉例
- 上一篇：pCuo CA Rac1
- 下一篇：pET-28b-RfxCas13d-His