| 質粒名稱: | pAAV-Syn-SomArchon |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 126941 |
| 存儲實驗室: | Edward Boyden |
|
載體骨架基本信息 |
|
| 載體名稱: | pAAV-Syn |
| 載體出品公司: | Clontech |
| 空載體大小: | 5279bp |
| 完整質粒大小: | 6265bp |
| 載體類型: | 哺乳細胞表達, AAV |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷貝數: | 未知 |
|
插入基因信息 |
|
| 插入基因名稱: | SomArchon |
| 物種來源: | Synthetic |
| 插入大小: | 1821bp |
| 啟動子: | Synapsin |
| 標簽/融合蛋白: | GFP |
| 克隆方法: | Gibson Cloning |
| 5’測序引物 |
gcacgggcgcgaccatctgc
(Common Sequencing Primers)
|
|
文獻引用方法 |
|
| 材料和方法部分: | pAAV-Syn-SomArchon was a gift from Edward Boyden (Addgene plasmid # 126941 ; //n2t.net/addgene:126941 ; RRID:Addgene_126941) |
| 參考文獻部分: | Population imaging of neural activity in awake behaving mice. Piatkevich KD, Bensussen S, Tseng HA, Shroff SN, Lopez-Huerta VG, Park D, Jung EE, Shemesh OA, Straub C, Gritton HJ, Romano MF, Costa E, Sabatini BL, Fu Z, Boyden ES, Han X. Nature. 2019 Oct;574(7778):413-417. doi: 10.1038/s41586-019-1641-1. Epub 2019 Oct 9. 10.1038/s41586-019-1641-1 PubMed 31597963 |
買家導航
pAAV-Syn-SomArchon載體質粒基本信息
pAAV-Syn-SomArchon質粒圖譜載體圖譜和pAAV-Syn-SomArchon載體序列質粒序列多克隆位點信息
pAAV-Syn-SomArchon質粒載體簡介
pAAV-Syn-SomArchon質粒序列載體序列
LOCUS pAAV-Syn-SomArchon 6522 bp DNA circular SYN 25-AUG-2024
DEFINITION Expression of SomArchon under Syn promoter.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6522)
AUTHORS Piatkevich KD, Bensussen S, Tseng HA, Shroff SN, Lopez-Huerta VG,
Park D, Jung EE, Shemesh OA, Straub C, Gritton HJ, Romano MF, Costa
E, Sabatini BL, Fu Z, Boyden ES, Han X
TITLE Population imaging of neural activity in awake behaving mice.
JOURNAL Nature. 2019 Oct;574(7778):413-417. doi: 10.1038/s41586-019-1641-1.
Epub 2019 Oct 9.
PUBMED 31597963
REFERENCE 2 (bases 1 to 6522)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1038/s41586-019-1641-1"; journalName: "Nature"; date: "2019-10";
volume: "574"; issue: "7778"; pages: "413-417"
FEATURES Location/Qualifiers
source 1..6522
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
promoter 341..788
/label=hSyn promoter
/note="human synapsin I promoter; confers neuron-specific
expression (Kugler et al., 2003)"
regulatory 801..810
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1674..2387
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
CDS 1674..2387
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(1716..1737)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1977..1996)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2324..2345
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 2655..3243
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2708..2728)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3126..3137)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3263..3739
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(3462..3481)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 3785..3925
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3785..3914
/label=AAV2 ITR
rep_origin 4000..4455
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4087..4106)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4297..4318
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4472..4491)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4591..4613
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4651..4669)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4737..4841
/gene="bla"
/label=AmpR promoter
CDS 4842..5702
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5060..5079)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5873..6461
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6362..6381
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc
181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca
301 aatggttaat taatctagac tgcagagggc cctgcgtatg agtgcaagtg ggttttagga
361 ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc
421 acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga
481 tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc
541 ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc
601 ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg
661 gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg
721 ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga
781 gagcgcagtc gagaggatcc gccaccatgg tttctatcgc actgcaggct ggttatgacc
841 tcctcggaga tggtagacct gaaagtctgt ggctcggaat tggtacactg ctgatgctga
901 tcggaacctt ctacttcctg gttagagcgt ggggagagac tgacaaggat gcacgggagt
961 actacgcagt gacaatcctg gtcagcggta ttgccagtgc agcttatctg tcaatgttct
1021 ttggtattgg cctcacagag gtgccagtag gtggagagat gctgaacatc tactacgcaa
1081 gatacgcaca gtggctgttc actacaccac tgttgctgct gcacctggca ctcctggcca
1141 aagttgatag agtgaccatc ggcactctcg ttggcgtcga tgctctgatg attgtcacag
1201 gcctgatagg aacactgtca catactgcca ttgccagata ctcttggtgg ctgttctcaa
1261 ctatctgtat gatcgtcgtt ctgtatgtcc tggccacttc tctgcggtca gccgctaagg
1321 agagaggccc agaggttgcc tcaaccttca acatcctcac cgcactggtt ctggtcctgt
1381 ggactgctta ccctatcatc tggattatcg gcaccgaggg tgccggagtt gtgggcctgg
1441 gaatcgaaac tttgctgttc atggtgctgg acgtcaccgc caaggtgggc ttcggtttta
1501 tcctgctcag gtcaagagcc attctgcaag atacagaagc acctgaacct tcagctggag
1561 ctgataccgg tgccgccgac cgcccggtag tagcagtgag caaggcggcc gccaagagca
1621 ggatcaccag cgagggcgag tacatccccc tggaccagat cgacatcaac gtggtgagca
1681 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
1741 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
1801 ccctgaagtt catttgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
1861 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
1921 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
1981 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
2041 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
2101 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
2161 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
2221 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
2281 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
2341 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggt gggagcggag
2401 gtacccagag ccagcctatc ctgaacacca aggagatggc ccctcagagc aagcctcctg
2461 aggagctgga gatgagcagc atgcctagcc ctgtggcccc tctgcctgcc aggaccgagg
2521 gcgtgatcga catgaggagc atgagcagca tcgacagctt catcagctgc gccaccgact
2581 tccctgaggc caccaggttc tacaagttct gctacgagaa cgaggtgtaa gaattcgata
2641 tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
2701 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
2761 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
2821 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
2881 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
2941 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
3001 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
3061 ttccttggct gctcgcctat gttgccacct ggattctgcg cgggacgtcc ttctgctacg
3121 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
3181 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
3241 cgcatcgata ccggctcgag acgggtggca tccctgtgac ccctccccag tgcctctcct
3301 ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat
3361 cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag
3421 caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg
3481 gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct
3541 cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt
3601 tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta
3661 atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac
3721 tgctcccttc cctgtccttc tcgagctgat tttgtaggta accacgtgcg gaccgagcgg
3781 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
3841 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
3901 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
3961 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
4021 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
4081 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
4141 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
4201 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
4261 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
4321 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
4381 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
4441 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
4501 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
4561 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
4621 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
4681 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
4741 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
4801 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
4861 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
4921 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
4981 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
5041 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
5101 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
5161 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
5221 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
5281 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
5341 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
5401 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
5461 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
5521 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
5581 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
5641 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
5701 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
5761 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
5821 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
5881 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
5941 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
6001 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
6061 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
6121 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
6181 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
6241 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
6301 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
6361 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
6421 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
6481 ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
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