| 產品名稱： | 寄生蟲細胞培養基；ATCC培養基 2222 |
|---|---|
| 英文名稱： | Cell cultivation medium for parasites；ATCC medium 2222 |
| 培養基類型： | 加富培養基 |
| 級別： | for microbiology |
| 品牌： | ELITE-MEDIA（艾禮培養基） |
| 產品目錄號： | M2854 |
| 產品規格： | 250g、500g |
| 產品外觀： | 乳白色至淺黃色粉末。 |
| 滅菌后顏色與澄清度： | 淺琥珀色，透明，無沉淀。 |
| 保存條件： | 密封，2-25°C保存。 |
| 注意事項： | 避免攝入、吸入、皮膚接觸。 |
| 相關產品： | -- |
產品描述：
胰蛋白胨大豆肉湯（TSB）培養基是加富培養基，用于營養需求高的微生物（難養菌）的培養和回收，不需要加血清。胰蛋白胨大豆肉湯培養基適用范圍廣泛，可用于培養好氧菌、厭氧菌和真菌。例如：
Bacillus subtilis 枯草芽孢桿菌
Bacteroides vulgatus 普通擬桿菌
Candida albicans 白色念珠菌
Escherichia coli 大腸桿菌
Micrococcus luteus 藤黃微球菌
Staphylococcus aureus 金黃色葡萄球菌
Staphylococcus epidermidis 表皮葡萄球菌
Streptococcus pneumonia 肺炎鏈球菌
Streptococcus pyogenes 釀膿鏈球菌
用途：
作為致病菌的富集培養基。用于配制血液培養基和血瓊脂培養基。
用于無菌試驗，檢測真菌和好氧菌污染。
在醫學上，常用來培養從臨床樣品中分離的致病微生物。
用于藥敏試驗和紙片擴散法試驗。
配方與配制方法
| Dulbecco's Minimal Essential Medium (DMEM)* | 450.0 ml |
| Fetal Bovine Serum (FBS)** | 50.0 ml |
將DMEM和冷卻的熱滅活血清無菌結合。
*ATCC Cat. #30-2002
**ATCC Cat. #30-2021
*ATCC Cat. #30-2002
**ATCC Cat. #30-2021
TCC測試的胎牛血清（FBS）可作為ATCC Cat#獲得30-2020（500毫升）和Cat.#30-2021（100毫升）。ATCC分布式FBS未進行熱滅活。
Thaw serum: Remove the serum from frozen storage and place it overnight in a refrigerator at 2C to 6C. Transfer the bottles to a 37C water bath for approximately 5 minutes. Agitate the bottles from time-to-time in order to mix the solutes that tend to concentrate at the bottom of the bottle. Do not keep the serum at 37C any longer than necessary to completely thaw it. Thawing serum in a bath above 40C without mixing may lead to the formation of a precipitate inside the bottle. We don't recommend thawing the serum at a high temperature. After the serum has warmed in the 37C water bath, continue with the heat-inactivation process. Heat-inactivate the FBS: Pre-heat water bath to 56C. There must be sufficient water to immerse the bottle above the level of serum. Mix thawed serum by gentle inversion and place serum bottle in the 56C water bath. (The temperature of the water bath will drop.) When the temperature of the water bath reaches 56C again, continue to heat for a additional 30 minutes. Mix gently every five minutes to insure uniform heating. Remove serum from water bath and cool. To avoid contamination, do not allow the level of the water in the bath to come in contact with the lip of the screw cap. It is best to leave one inch between the serum level in the bottle and the lip of the cap and to fill the water bath to a level just slightly above the level of the serum.
Thaw serum: Remove the serum from frozen storage and place it overnight in a refrigerator at 2C to 6C. Transfer the bottles to a 37C water bath for approximately 5 minutes. Agitate the bottles from time-to-time in order to mix the solutes that tend to concentrate at the bottom of the bottle. Do not keep the serum at 37C any longer than necessary to completely thaw it. Thawing serum in a bath above 40C without mixing may lead to the formation of a precipitate inside the bottle. We don't recommend thawing the serum at a high temperature. After the serum has warmed in the 37C water bath, continue with the heat-inactivation process. Heat-inactivate the FBS: Pre-heat water bath to 56C. There must be sufficient water to immerse the bottle above the level of serum. Mix thawed serum by gentle inversion and place serum bottle in the 56C water bath. (The temperature of the water bath will drop.) When the temperature of the water bath reaches 56C again, continue to heat for a additional 30 minutes. Mix gently every five minutes to insure uniform heating. Remove serum from water bath and cool. To avoid contamination, do not allow the level of the water in the bath to come in contact with the lip of the screw cap. It is best to leave one inch between the serum level in the bottle and the lip of the cap and to fill the water bath to a level just slightly above the level of the serum.