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pEX-C-His載體質粒基本信息
pEX-C-His質粒圖譜載體圖譜和pEX-C-His載體序列質粒序列多克隆位點信息
pEX-C-His質粒載體簡介
pEX-C-His質粒序列載體序列
LOCUS pEX-C-His 4583 bp DNA circular SYN 12-NOV-2024
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4583)
AUTHORS Ming
TITLE Direct Submission
JOURNAL Exported Nov 12, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
//gt12365.cn
COMMENT VNTDATE|1073779200|
COMMENT VNTDBDATE|1073779200|
COMMENT VNTNAME|pEX-C-His|
COMMENT VNTAUTHORNAME|Ming|
FEATURES Location/Qualifiers
primer_bind 155..171
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 207..225
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 226..250
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 254..276
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 338..358
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
CDS 359..376
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
CDS 395..415
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
CDS 416..433
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
terminator 488..534
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 780..857
/gene="lacI"
/label=lacI promoter
CDS 858..1940
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter 1989..2019
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2027..2043
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2051..2067
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2083..2099)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(2391..2979)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3150..4010)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
promoter complement(4011..4115)
/gene="bla"
/label=AmpR promoter
rep_origin 4142..4570
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 aacgcttaca atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
61 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
121 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccgaattc
181 tgctggactt tccaaaatgt cgagcttaat acgactcact ataggggaat tgtgagcgga
241 taacaattcc ccttttgttt aactttaaga aggagatatc gatcgctctc ctcggcgcgc
301 cagatctcaa gcttaactag ctagcggacc gacgcgtgaa aacctgtatt ttcagggcca
361 tcaccatcac catcactaaa cgcggccgct cgaggaaaac ctgtattttc agggccatca
421 ccatcaccat cacgtttaaa cggccggcca catgactgac tgagccacga taaccgctga
481 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttcccacc
541 agccttgtcc taatccgcgg cggttcctgg ccttttgctg gccttttgct cacatgttct
601 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
661 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
721 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataaattccg
781 acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc
841 aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg
901 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc
961 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac
1021 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg
1081 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg
1141 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc
1201 tcgcgcaacg agtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg
1261 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac
1321 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg
1381 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc
1441 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg
1501 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg
1561 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc
1621 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg
1681 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc
1741 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
1801 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc
1861 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
1921 gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcac tcattaggca
1981 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
2041 caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca
2101 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
2161 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat ggatccgctc ttccgcttcc
2221 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
2281 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
2341 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
2401 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
2461 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
2521 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
2581 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
2641 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
2701 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
2761 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
2821 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
2881 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
2941 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
3001 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
3061 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
3121 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
3181 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
3241 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
3301 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
3361 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
3421 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
3481 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
3541 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
3601 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
3661 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
3721 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
3781 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
3841 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
3901 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
3961 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
4021 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
4081 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
4141 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
4201 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
4261 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
4321 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
4381 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
4441 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
4501 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
4561 aacgcgaatt ttaacaaaat att
//
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