LOCUS       pGEX-2T	4948 bp 	DNA       circular   	SYN
DEFINITION  pGEX-2T
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            //gt12365.cn/
COMMENT     VNTAUTHORNAME|gt12365.cn|
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="pGEX-2T"
                     /mol_type="other DNA"
     promoter        184..212
                     /label="tac_promoter"
     misc_feature    224..246
                     /label="M13_pUC_rev_primer"
     gene            258..956
                     /label="GST (variant)"
                     /gene="GST (variant)"
     CDS             258..956
                     /label="ORF frame 3"
     misc_feature    complement(998..1020)
                     /label="pGEX_3_primer"
     promoter        1286..1314
                     /label="AmpR_promoter"
     gene            1356..2216
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             1356..2216
                     /label="ORF frame 3"
     rep_origin      2371..2990
                     /label="pBR322_origin"
     misc_feature    3288..4379
                     /label="lacI"
     CDS             3420..4379
                     /label="ORF frame 3"
     promoter        4428..4457
                     /label="lac_promoter"
     misc_feature    4471..4493
                     /label="M13_pUC_rev_primer"
     promoter        4492..4510
                     /label="M13_reverse_primer"
     CDS             4502..81
                     /label="ORF frame 2"
     misc_feature    4519..4674
                     /label="lacZ_a"
     promoter        complement(4522..4538)
                     /label="M13_forward20_primer"
     misc_feature    complement(4531..4553)
                     /label="M13_pUC_fwd_primer"
ORIGIN
    1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
   61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
  121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
  181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
  241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
  301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
  361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
  421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
  481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
  541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
  601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
  661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
  721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
  781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
  841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
  901 atcctccaaa atcggatctg gttccgcgtg gatccccggg aattcatcgt gactgactga
  961 cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
 1021 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
 1081 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
 1141 tgtataattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
 1201 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
 1261 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
 1321 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
 1381 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
 1441 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
 1501 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
 1561 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
 1621 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
 1681 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
 1741 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
 1801 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
 1861 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
 1921 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
 1981 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
 2041 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
 2101 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
 2161 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
 2221 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
 2281 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
 2341 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
 2401 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
 2461 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
 2521 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
 2581 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
 2641 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
 2701 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
 2761 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
 2821 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
 2881 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
 2941 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
 3001 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
 3061 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
 3121 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
 3181 ttacgcatct gtgcggtatt tcacaccgca taaattccga caccatcgaa tggtgcaaaa
 3241 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga
 3301 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
 3361 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
 3421 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
 3481 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
 3541 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
 3601 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
 3661 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
 3721 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
 3781 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
 3841 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
 3901 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
 3961 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga
 4021 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
 4081 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
 4141 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
 4201 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
 4261 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt
 4321 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
 4381 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
 4441 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
 4501 tatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
 4561 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
 4621 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
 4681 ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc
 4741 tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat
 4801 ctacaccaac gtaacctatc ccattacggt caatccgccg tttgttccca cggagaatcc
 4861 gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac
 4921 gcgaattatt tttgatggcg ttggaatt
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