LOCUS       pGEX-KG                 5006 bp    DNA     circular SYN 23-SEP-2012
DEFINITION  pGEX-KG.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            //gt12365.cn/
COMMENT     VNTAUTHORNAME|gt12365.cn|
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="pGEX-KG"
                     /mol_type="other DNA"
                     /vntifkey="98"
     promoter        184..212
                     /vntifkey="30"
                     /label=tac_promoter
     misc_feature    224..246
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     CDS             258..1010
                     /vntifkey="4"
                     /label=ORF\frame\3
     misc_feature    258..1014
                     /vntifkey="21"
                     /label=GST
     misc_feature    869..891
                     /vntifkey="21"
                     /label=pGEX_5_primer
     misc_feature    complement(1056..1078)
                     /vntifkey="21"
                     /label=pGEX_3_primer
     promoter        1344..1372
                     /vntifkey="30"
                     /label=AmpR_promoter
     CDS             1414..2274
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 1"
     gene            1414..2274
                     /gene="Ampicillin"
                     /vntifkey="60"
     rep_origin      2429..3048
                     /vntifkey="33"
                     /label=pBR322_origin
     misc_feature    3346..4437
                     /vntifkey="21"
                     /label=lacI
     CDS             3478..4437
                     /vntifkey="4"
                     /label=ORF\frame\1
     promoter        4486..4515
                     /vntifkey="30"
                     /label=lac_promoter
     misc_feature    4529..4551
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     misc_feature    4550..4568
                     /vntifkey="21"
                     /label=M13_reverse_primer
     misc_feature    complement(4580..4596)
                     /vntifkey="21"
                     /label=M13_forward20_primer
     misc_feature    complement(4589..4611)
                     /vntifkey="21"
                     /label=M13_pUC_fwd_primer
     misc_feature    4577..4732
                     /vntifkey="21"
                     /label=lacZ_a
BASE COUNT     1234 a      1205 c      1302 g      1265 t 
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 
      901 atcctccaaa atcggatctg gttccgcgtg gatccccggg aatttccggt ggtggtggtg 
      961 gaattctaga ctccatgggt cgactcgagc tcaagcttaa ttcatcgtga ctgactgacg 
     1021 atctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 
     1081 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 
     1141 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg 
     1201 tataattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg 
     1261 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 
     1321 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 
     1381 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 
     1441 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 
     1501 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 
     1561 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 
     1621 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc 
     1681 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 
     1741 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 
     1801 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 
     1861 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 
     1921 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc 
     1981 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 
     2041 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 
     2101 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 
     2161 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 
     2221 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 
     2281 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 
     2341 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 
     2401 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 
     2461 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 
     2521 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 
     2581 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 
     2641 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 
     2701 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 
     2761 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 
     2821 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 
     2881 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 
     2941 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 
     3001 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 
     3061 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 
     3121 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 
     3181 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt 
     3241 acgcatctgt gcggtatttc acaccgcata aattccgaca ccatcgaatg gtgcaaaacc 
     3301 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa 
     3361 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc 
     3421 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg 
     3481 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg 
     3541 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg 
     3601 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc 
     3661 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg 
     3721 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat 
     3781 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc 
     3841 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc 
     3901 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat 
     3961 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc 
     4021 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg 
     4081 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg 
     4141 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat 
     4201 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac 
     4261 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca 
     4321 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 
     4381 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 
     4441 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 
     4501 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta 
     4561 tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 
     4621 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 
     4681 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct 
     4741 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg 
     4801 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct 
     4861 acaccaacgt aacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga 
     4921 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc 
     4981 gaattatttt tgatggcgtt ggaatt 
//