LOCUS       pIN III ompA3	7025 bp 	DNA	SYN
DEFINITION  pIN III ompA3
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="pIN III ompA3"
                     /mol_type="other DNA"
     promoter        269..292
                     /label="lac_promoter"
     misc_feature    312..334
                     /label="M13_pUC_rev_primer"
     promoter        333..351
                     /label="M13_reverse_primer"
     CDS             complement(648..1253)
                     /label="ORF frame 1"
                     /translation="METRVGQKIPNPTWTPTAGIRQRSLERGIKLPPVVPAGPNNPLG
                     RYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIKALFSSVRTGTPVKVINE
                     PVKYSVEPNGMRYVEVHRPLSAGEQQNVQTMPYTLPAGFTQFKDNKAVDQKLVDKALY
                     RRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRVTQ*"
     misc_feature    1422..2513
                     /label="lacI"
                     /translation="METRVGQKIPNPTWTPTAGIRQRSLERGIKLPPVVPAGPNNPLG
                     RYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIKALFSSVRTGTPVKVINE
                     PVKYSVEPNGMRYVEVHRPLSAGEQQNVQTMPYTLPAGFTQFKDNKAVDQKLVDKALY
                     RRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRVTQ*"
     CDS             1554..2513
                     /label="ORF frame 3"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             complement(3207..3779)
                     /label="ORF frame 1"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSPSVCQNYATKTEVHKIA
                     FRAVRVRCGLSCARMPRELLNYRATAMRRL*"
     CDS             3299..3772
                     /label="ORF frame 2"
                     /translation="MLFCGLLSSSRNSGRRWGTDALESLQPSQLLPVGAGHDYRRRTY
                     DCLLYHATRRTGAGSALGHFRRGPLSLERDDDRPVACGIRNLARPRSSLRHWSRHQTF
                     RREAGHYRRHGGRRAGLRLAGVRDARLDGLPHYDSSRFRRHRDARVAGHAVQAGR*"
     CDS             3357..3974
                     /label="ORF frame 3"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     gene            3360..3974
                     /label="tet (576 - 1190)"
                     /gene="tet (576 - 1190)"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     misc_feature    4613..4804
                     /label="ROP"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     misc_feature    complement(4798..4820)
                     /label="pGEX_3_primer"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     gene            complement(5991..6851)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI
                     FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL
                     LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG
                     PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
     CDS             complement(5991..6851)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(6893..6921)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 taggcgtatc acgaggccct ttggataacc agaagcaata aaaaatcaaa tcggatttca
   61 ctatataatc tcactttatc taagatgaat ccgatggaag catcctgttt tctctcaatt
  121 tttttatcta aaacccagcg ttcgatgctt ctttgagcga acgatcaaaa ataagtgcct
  181 tcccatcaaa aaaatattct caacataaaa aactttgtgt aatacttgta acgctacatg
  241 gagattaact caatctagct agagaggctt tacactttat gcttccggct cgtataatgt
  301 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacggat
  361 tcactggaac tctagataac gagggcaaaa aatgaaaaag acagctatcg cgattgcagt
  421 ggcactggct ggtttcgcta ccgtagcgca ggccggaatt ccaagcttgg atccggctga
  481 gcaacgacgt gaacgcaatg cgttccgacg ttcaggctgc taaagatgac gcagctcgtg
  541 ctaaccagcg tctggacaac atggctacta aataccgcaa gtaatagtac ctgtgaagtg
  601 aaaaatggcg cacattgtgc gacatttttt ttgtctgccg tttaccgcta ctgcgtcacg
  661 cgtaacatat tcccttgctc tggttcacca ttctgcgctg actctactga aggcgcattg
  721 ctggctgcgg gagttgctcc actgctcacc gaaaccggat accctgcccg acgatacaac
  781 gctttatcga ctaacttctg atctacagcc ttattgtctt taaattgcgt aaagcctgct
  841 ggcagtgtgt atggcattgt ctgaacgttc tgctgttctc ctgccgatag tggtcgatgt
  901 acttcaacat aacgcatccc gttaggctcc acggaatatt tcaccggttc gttgatcact
  961 ttcaccggcg ttcccgtccg cacgctggag aacaaggctt taatatccgg tgcattcatg
 1021 cgaatacacc ctgaactgac gcgcaaaccg acgctgtccg gcgcactggt accatgaatg
 1081 aggtattcgc cattaccatg cgcgaggcgc agtgcgtaac gtcctagcgg gttatttggt
 1141 ccggcaggaa cgactggcgg taatttaatg ccacgctcca gcgaacgctg acgaatgcct
 1201 gccgtaggcg tccaggttgg gttagggatt ttctgcccaa cacgcgtttc catcaccggc
 1261 gtttccagcc cctgcaatcc aatacctatt ggataaacct gcacaatatt ttctcccggc
 1321 ggataataat aaaggcgcag ctctgcaagg ttgacaccat cgaatggcgc aaaacctttc
 1381 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
 1441 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
 1501 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
 1561 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
 1621 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
 1681 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
 1741 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
 1801 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
 1861 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
 1921 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
 1981 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
 2041 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
 2101 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
 2161 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
 2221 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
 2281 cgccgtcaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
 2341 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
 2401 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
 2461 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
 2521 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgctaacg
 2581 ataatcccct gacgcggtgc atcaggtaat aacagttgtg aaggaatagt tatcgtcgta
 2641 ccaggttttg gcaccggggc gatagtgtta ttggcttcaa ggatcaacat tgccgcagta
 2701 tcaaaacgtc gggcaatagc ctgaaggttt ttatcccctt cttgcaccgt atacgtttga
 2761 ttttgcccaa ccagtcggct tccggttggt ggtagcggat aatcaaccgc ccaggcagcc
 2821 tggatggcgc taaaagcgcc gataagcgtg agtgtaagca aagacgcgcg tttcattgta
 2881 aacctcctgt atttgccgga gactcacgct gaaacgtcgg atggcgctta tgttcacctg
 2941 aaaccaaaac actcctgtgc aggtcagtgt aaacattgac catccggcaa tgtgagccaa
 3001 ccggatgaaa gctgtccttt tagtttagct aagtgcagcg gctttggcgc gaattgcgcg
 3061 aatcatcgct tccagacctt gtgaacgaga tggggtgaga tgttgggtga gcgccatttt
 3121 ttcaaaccac ggacgcacat cgaaattgac aatatcctgc ggcgtcatct gatcgtagag
 3181 aataaagacg accgcaataa gccctttcac aatcgccgca tcgctgtcgc cctgtaattc
 3241 aataattccc tgggcattct ggcgcatgac aatccacacc tgactctgac agccctgaat
 3301 gctattttgt ggacttctgt cttcgtcgcg taattctggc agacgctggg ggaccgatgc
 3361 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
 3421 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
 3481 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
 3541 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
 3601 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
 3661 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
 3721 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
 3781 agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
 3841 tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
 3901 taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
 3961 ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
 4021 tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
 4081 atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
 4141 ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
 4201 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
 4261 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
 4321 aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
 4381 atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
 4441 ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
 4501 ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
 4561 catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa
 4621 acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat taacgcttct
 4681 ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga
 4741 ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
 4801 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
 4861 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
 4921 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
 4981 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
 5041 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
 5101 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
 5161 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
 5221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
 5281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
 5341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
 5401 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
 5461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
 5521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
 5581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
 5641 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
 5701 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
 5761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
 5821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
 5881 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
 5941 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
 6001 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
 6061 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
 6121 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
 6181 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
 6241 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
 6301 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
 6361 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
 6421 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
 6481 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
 6541 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
 6601 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
 6661 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
 6721 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
 6781 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
 6841 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
 6901 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
 6961 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
 7021 aaaaa
//