LOCUS       pSPL3	6031 bp 	DNA	SYN
DEFINITION  pSPL3
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="pSPL3"
                     /mol_type="other DNA"
     misc_feature    complement(5..25)
                     /label="pBABE_3_primer"
     misc_feature    complement(11..226)
                     /label="SV40_enhancer"
     promoter        23..291
                     /label="SV40_promoter"
     rep_origin      190..267
                     /label="SV40_origin"
     misc_feature    252..271
                     /label="SV40pro_F_primer"
     CDS             875..3262
                     /label="ORF frame 2"
                     /translation="MRVKEKYQHLWRWGWRWGTMLLGMLMICSATEKLWVTVYYGVRD
                     HQNSGARAAAAGSQISGDPVPVWKEATTTLFCASDAKAYDTEVHNVWATHAGVPTDPN
                     PQEVVLVNVTENFNMWKNDMVEQMHEDIISLWDQSLKPCVKLTPLCVSLKCTDLKNDT
                     NTNSSSGRMIMEKGEIKNCSFNISTSIRGKVQKEYAFFYKLDIIPIDNDTTSYTLTSC
                     NTSVITQACPKVSFEPIPIHYCAPAGFAILKCNNKTFNGTGPCTNVSTVQCTHGIRPV
                     VSTQLLLNGSLAEEEVVIRSVNFTDNAKTIIVQLNTSVEINCTRPNNNTRKKIRIQRG
                     PGRAFVTIGKIGNMRQAHCNISRAKWNATLKQIASKLREQFGNNKTIIFKQSSGGDPE
                     IVTHSFNCGGEFFYCNSTQLFNSTWFNSTWSTEGSNNTEGSDTITLPCRIKQFINMWQ
                     EVGKAMYAPPISGQIRCSSNITGLLLTRDGGNNNNGSEIFRPGGGDMRDNWRSELYKY
                     KVVKIEPLGVAPTKAKRRVVQREKRAVGIGALFLGFLGAAGSTMGAASMTLTVQARQL
                     LSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQARILAVERYLKDQQLLGIWGCSGKL
                     LCTTAVPWNASWSNKSLEQIWNHTTWMEWDREINNYTSLIHSLIEESQNQQEKNEQEL
                     LELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLRIVFAVLSVVNRVRQGYSPLSFQ
                     TWRSPEGTRQARRNRRRRWRERQRQIHFDQFTPQVQAAYQKVVAGVANALAHKYH*"
     CDS             complement(2089..2658)
                     /label="ORF frame 2"
                     /translation="MPQTVSCNRCCCASIALSKLFCCCTIPDNNCLACTVSVIDAAPI
                     VLPAAPKNPRNKAPIPTALFSLCTTLLFALVGATPNGSIFTTLYLYNSLLQLSLISPP
                     PGLKISDPLLLLPPSLVNSSPVIFDEHLICPLMGGAYIAFPTSCHMFINCFILHGSVI
                     VSLPSVLFDPSVLQVLLNQVLLNSCVELQ*"
     misc_feature    2485..2718
                     /label="RRE"
                     /translation="MPQTVSCNRCCCASIALSKLFCCCTIPDNNCLACTVSVIDAAPI
                     VLPAAPKNPRNKAPIPTALFSLCTTLLFALVGATPNGSIFTTLYLYNSLLQLSLISPP
                     PGLKISDPLLLLPPSLVNSSPVIFDEHLICPLMGGAYIAFPTSCHMFINCFILHGSVI
                     VSLPSVLFDPSVLQVLLNQVLLNSCVELQ*"
     terminator      complement(3261..3392)
                     /label="SV40_PA_terminator"
                     /translation="MPQTVSCNRCCCASIALSKLFCCCTIPDNNCLACTVSVIDAAPI
                     VLPAAPKNPRNKAPIPTALFSLCTTLLFALVGATPNGSIFTTLYLYNSLLQLSLISPP
                     PGLKISDPLLLLPPSLVNSSPVIFDEHLICPLMGGAYIAFPTSCHMFINCFILHGSVI
                     VSLPSVLFDPSVLQVLLNQVLLNSCVELQ*"
     misc_feature    complement(3287..3306)
                     /label="EBV_rev_primer"
                     /translation="MPQTVSCNRCCCASIALSKLFCCCTIPDNNCLACTVSVIDAAPI
                     VLPAAPKNPRNKAPIPTALFSLCTTLLFALVGATPNGSIFTTLYLYNSLLQLSLISPP
                     PGLKISDPLLLLPPSLVNSSPVIFDEHLICPLMGGAYIAFPTSCHMFINCFILHGSVI
                     VSLPSVLFDPSVLQVLLNQVLLNSCVELQ*"
     CDS             complement(3420..3965)
                     /label="ORF frame 3"
                     /translation="MNTDHKAVLDKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPP
                     VLHITNTATTVLLDEQGVGPLCKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITL
                     RKRSVKNPYPISFLLSDLINRRTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDHNQPY
                     HICRGFTCFKKPPTPPPEPET*"
     promoter        4106..4134
                     /label="AmpR_promoter"
                     /translation="MNTDHKAVLDKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPP
                     VLHITNTATTVLLDEQGVGPLCKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITL
                     RKRSVKNPYPISFLLSDLINRRTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDHNQPY
                     HICRGFTCFKKPPTPPPEPET*"
     gene            4176..5036
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MNTDHKAVLDKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPP
                     VLHITNTATTVLLDEQGVGPLCKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITL
                     RKRSVKNPYPISFLLSDLINRRTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDHNQPY
                     HICRGFTCFKKPPTPPPEPET*"
     CDS             4176..5036
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5191..5810
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT
   61 ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA
  121 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA
  181 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA
  241 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG
  301 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTGGACT GTGTTTACTT
  361 GCAATCCCCC AAAACAGACA GAATGGTGCA TCTGTCCAGT GAGGAGAAGT CTGCGGTCAC
  421 TGCCCTGTGG GGCAAGGTGA ATGTGGAAGA AGTTGGTGGT GAGGCCCTGG GCAGGCTGCT
  481 GGTTGTCTAC CCATGGACCC AGAGGTTCTT CGAGTCCTTT GGGGACCTGT CCTCTGCAAA
  541 TGCTGTTATG AACAATCCTA AGGTGAAGGC TCATGGCAAG AAGGTGCTGG CTGCCTTCAG
  601 TGAGGGTCTG AGTCACCTGG ACAACCTCAA AGGCACCTTT GCTAAGCTGA GTGAACTGCA
  661 CTGTGACAAG CTGCACGTGC TCTAGAGTCG ACCCAGCAGT AAGTAATACA TGTAATGCAA
  721 CCTATACAAA TAGCAATAGT AGCATTAGTA GTAGCAATAA TAATAGCAAT AGTTGTGTGG
  781 TCCATAGTAA TCATAGAATA TAGGAAAATA TTAAGACAAA GAAAAATAGA CAGGTTAATT
  841 GATAGACTAA TAGAAAGAGC AGAAGACAGT GGCAATGAGA GTGAAGGAGA AATATCAGCA
  901 CTTGTGGAGA TGGGGGTGGA GATGGGGCAC CATGCTCCTT GGGATGTTGA TGATCTGTAG
  961 TGCTACAGAA AAATTGTGGG TCACAGTCTA TTATGGGGTA CGGGATCACC AGAATTCTGG
 1021 AGCTCGAGCG GCCGCTGCAG GATCCCAGAT ATCTGGTGAT CCCGTACCTG TGTGGAAGGA
 1081 AGCAACCACC ACTCTATTTT GTGCATCAGA TGCTAAAGCA TATGATACAG AGGTACATAA
 1141 TGTTTGGGCC ACACATGCCG GTGTACCCAC AGACCCCAAC CCACAAGAAG TAGTATTGGT
 1201 AAATGTGACA GAAAATTTTA ACATGTGGAA AAATGACATG GTAGAACAGA TGCATGAGGA
 1261 TATAATCAGT TTATGGGATC AAAGCCTAAA GCCATGTGTA AAATTAACCC CACTCTGTGT
 1321 TAGTTTAAAG TGCACTGATT TGAAGAATGA TACTAATACC AATAGTAGTA GCGGGAGAAT
 1381 GATAATGGAG AAAGGAGAGA TAAAAAACTG CTCTTTCAAT ATCAGCACAA GCATAAGAGG
 1441 TAAGGTGCAG AAAGAATATG CATTTTTTTA TAAACTTGAT ATAATACCAA TAGATAATGA
 1501 TACTACCAGC TATACGTTGA CAAGTTGTAA CACCTCAGTC ATTACACAGG CCTGTCCAAA
 1561 GGTATCCTTT GAGCCAATTC CCATACATTA TTGTGCCCCG GCTGGTTTTG CGATTCTAAA
 1621 ATGTAATAAT AAGACGTTCA ATGGAACAGG ACCATGTACA AATGTCAGCA CAGTACAATG
 1681 TACACATGGA ATTAGGCCAG TAGTATCAAC TCAACTGCTG TTAAATGGCA GTCTAGCAGA
 1741 AGAAGAGGTA GTAATTAGAT CTGTCAATTT CACGGACAAT GCTAAAACCA TAATAGTACA
 1801 GCTGAACACA TCTGTAGAAA TTAATTGTAC AAGACCCAAC AACAATACAA GAAAAAAAAT
 1861 CCGTATCCAG AGGGGACCAG GGAGAGCATT TGTTACAATA GGAAAAATAG GAAATATGAG
 1921 ACAAGCACAT TGTAACATTA GTAGAGCAAA ATGGAATGCC ACTTTAAAAC AGATAGCTAG
 1981 CAAATTAAGA GAACAATTTG GAAATAATAA AACAATAATC TTTAAGCAAT CCTCAGGAGG
 2041 GGACCCAGAA ATTGTAACGC ACAGTTTTAA TTGTGGAGGG GAATTTTTCT ACTGTAATTC
 2101 AACACAACTG TTTAATAGTA CTTGGTTTAA TAGTACTTGG AGTACTGAAG GGTCAAATAA
 2161 CACTGAAGGA AGTGACACAA TCACACTCCC ATGCAGAATA AAACAATTTA TAAACATGTG
 2221 GCAGGAAGTA GGAAAAGCAA TGTATGCCCC TCCCATCAGC GGACAAATTA GATGTTCATC
 2281 AAATATTACA GGGCTGCTAT TAACAAGAGA TGGTGGTAAT AACAACAATG GGTCCGAGAT
 2341 CTTCAGACCT GGAGGAGGAG ATATGAGGGA CAATTGGAGA AGTGAATTAT ATAAATATAA
 2401 AGTAGTAAAA ATTGAACCAT TAGGAGTAGC ACCCACCAAG GCAAAGAGAA GAGTGGTGCA
 2461 GAGAGAAAAA AGAGCAGTGG GAATAGGAGC TTTGTTCCTT GGGTTCTTGG GAGCAGCAGG
 2521 AAGCACTATG GGCGCAGCGT CAATGACGCT GACGGTACAG GCCAGACAAT TATTGTCTGG
 2581 TATAGTGCAG CAGCAGAACA ATTTGCTGAG GGCTATTGAG GCGCAACAGC ATCTGTTGCA
 2641 ACTCACAGTC TGGGGCATCA AGCAGCTCCA GGCAAGAATC CTGGCTGTGG AAAGATACCT
 2701 AAAGGATCAA CAGCTCCTGG GGATTTGGGG TTGCTCTGGA AAACTACTTT GCACCACTGC
 2761 TGTGCCTTGG AATGCTAGTT GGAGTAATAA ATCTCTGGAA CAGATTTGGA ATCACACGAC
 2821 GTGGATGGAG TGGGACAGAG AAATTAACAA TTACACAAGC TTAATACACT CCTTAATTGA
 2881 AGAATCGCAA AACCAGCAAG AAAAGAATGA ACAAGAATTA TTGGAATTAG ATAAATGGGC
 2941 AAGTTTGTGG AATTGGTTTA ACATAACAAA TTGGCTGTGG TATATAAAAT TATTCATAAT
 3001 GATAGTAGGA GGCTTGGTAG GTTTAAGAAT AGTTTTTGCT GTACTTTCTG TAGTGAATAG
 3061 AGTTAGGCAG GGATATTCAC CATTATCGTT TCAGACCTGG AGATCTCCCG AGGGGACCCG
 3121 ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTTCGA
 3181 CCAATTCACT CCTCAGGTGC AGGCTGCCTA TCAGAAGGTG GTGGCTGGTG TGGCCAATGC
 3241 CCTGGCTCAC AAATACCACT GAGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA
 3301 AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC
 3361 TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT
 3421 TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA
 3481 ATGTGGTATG GCTGATTATG ATCCCCAGGA AGCTCCTCTG TGTCCTCATA AACCCTAACC
 3541 TCCTCTACTT GAGAGGACAT TCCAATCATA GGCTGCCCAT CCACCCTCTG TGTCCTCCTG
 3601 TTAATTAGGT CACTTAACAA AAAGGAAATT GGGTAGGGGT TTTTCACAGA CCGCTTTCTA
 3661 AGGGTAATTT TAAAATATCT GGGAAGTCCC TTCCACTGCT GTGTTCCAGA AGTGTTGGTA
 3721 AACAGCCCAC AAATGTCAAC AGCAGAAACA TACAAGCTGT CAGCTTTGCA CAAGGGCCCA
 3781 ACACCCTGCT CATCAAGAAG CACTGTGGTT GCTGTGTTAG TAATGTGCAA AACAGGAGGC
 3841 ACATTTTCCC CACCTGTGTA GGTTCCAAAA TATCTAGTGT TTTCATTTTT ACTTGGATCA
 3901 GGAACCCAGC ACTCCACTGG ATAAGCATTA TCCTTATCCA AAACAGCCTT GTGGTCAGTG
 3961 TTCATCTGCT GACTGTCAAC TGTAGCATTT TTTGGGGTTA CAGTTTGAGC AGGATATTTG
 4021 GTCCTGTAGT TTGCTAACAC ACCCCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC
 4081 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT
 4141 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG
 4201 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG
 4261 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC
 4321 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA
 4381 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC
 4441 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA
 4501 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG
 4561 ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT
 4621 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG
 4681 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC
 4741 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA
 4801 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA
 4861 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC
 4921 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG
 4981 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT
 5041 CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA
 5101 GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCTTAAC GGTGAGTTTT
 5161 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT
 5221 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT
 5281 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA
 5341 TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG
 5401 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA
 5461 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG
 5521 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA
 5581 GATACCTACA GCGCGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA
 5641 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA
 5701 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT
 5761 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC
 5821 GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT
 5881 CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA
 5941 CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC
 6001 TCCCCGCGCG TTGGCCGATT CATTAATGCA G
//