| 出品公司: | Novangen |
|---|---|
| 載體名稱: | pET-23(+), pET23 |
| 質粒類型: | 大腸桿菌表達載體；蛋白純化 |
| 高拷貝/低拷貝: | 低拷貝 |
| 克隆方法: | 多克隆位點；限制性內切酶 |
| 啟動子: | T7/ lacI |
| 載體大小: | 3592 bp |
| 5' 測序引物及序列: | T7 Fwd: 5'd[TAATACGACTCACTATAGGG]3' |
| 3' 測序引物及序列: | -- |
| 載體標簽: | His Tag （C-端） |
| 載體抗性: | 氨芐青霉素（Ampicillin） |
| 克隆菌株: | DH5a |
| 表達菌株: | BL21系列 |
| 備注: | pET-23a，b，c僅閱讀框（讀碼框）不同，彼此相差1個bp。 |
| 產品目錄號: | 71553-3 |
| 穩定性: | 穩表達 |
| 組成型/誘導型: | 誘導型（IPTG) |
| 病毒/非病毒: | 非病毒 |
| 表達宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表達手冊： |
pET表達手冊英文版 pET表達手冊中文版 |
| pET載體匯總: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
買家導航
pET-23(+)載體 pET23質粒基本信息
pET-23(+)質粒圖譜 pET23載體圖譜和pET-23(+)載體序列 pET23質粒序列多克隆位點信息
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 129 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| T7_promoter | 245 | 227 | |
| ROP | 754 | 945 | |
| pGEX_3_primer | 961 | 939 | |
| pBR322_origin | 1979 | 1360 | |
| Ampicillin | 2994 | 2134 | |
| AmpR_promoter | 3064 | 3036 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 2 | 2994 | 2134 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 158 |
| NotI | 166 |
| EagI | 166 |
| HindIII | 173 |
| SalI | 179 |
| SacI | 190 |
| EcoRI | 192 |
| BamHI | 198 |
| BglII | 260 |
| MscI | 285 |
| PvuII | 905 |
| PstI | 2452 |
pET-23(+)質粒 pET23載體簡介
pET-23系列載體是一種轉錄載體，設計用于從克隆插入位點中攜帶的細菌翻譯信號表達。因此，它缺乏存在于pET翻譯載體上的核糖體結合位點和ATG起始密碼子。pET-23系列載體具有C端T7His標簽。獨特的酶切位點在載體圖譜中被標注出來。需要注意的是，這些載體序列是依據載體pBR322的方向來進行編號的，所以T7標簽表達區域在載體圖譜上是反向的。
pET-23(+)質粒序列 pET23載體序列
LOCUS pET-23(+) 3592 bp DNA SYN
DEFINITION pET-23(+)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
//gt12365.cn/
COMMENT ORIGDB|GenBank|
COMMENT VNTAUTHORNAME|gt12365.cn|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..3592
/organism="pET-23(+)"
/mol_type="other DNA"
terminator complement(1..129)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
promoter complement(227..245)
/label="T7_promoter"
misc_feature 754..945
/label="ROP"
misc_feature complement(939..961)
/label="pGEX_3_primer"
rep_origin complement(1360..1979)
/label="pBR322_origin"
gene complement(2134..2994)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(2134..2994)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(3036..3064)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tcctagaggg aaaccgttgt ggtctcccta tagtgagtcg
241 tattaatttc gcgggatcga gatctcgggc agcgttgggt cctggccacg ggtgcgcatg
301 atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag
361 aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc
421 tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag
481 tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt
541 ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg
601 tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt
661 tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc
721 atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt
781 aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg
841 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac
901 cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
961 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
1021 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
1081 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat
1141 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
1201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
1261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
1321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
1381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
1441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
1501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
1561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
1621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
1681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
1741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
1801 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
1861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
1921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
1981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
2041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
2101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
2161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
2221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
2281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
2341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
2401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
2461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
2521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
2581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
2641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
2701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
2761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
2821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
2881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
2941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
3001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
3061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
3121 acctgaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
3181 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
3241 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
3301 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
3361 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
3421 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
3481 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
3541 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc ca
//
pET-23(+)載體圖譜 pET23質粒圖譜pdf版和pET-23(+)載體序列 pET23質粒序列等相關資料下載
pET-23(+)質粒 pET23載體應用舉例
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