| 出品公司: | EMD Biosciences(Novagen) |
|---|---|
| 別名: | pET23a, pet 23a |
| 質粒類型: | 大腸桿菌蛋白表達 |
| 表達水平: | 高 |
| 克隆方法: | 多克隆位點，限制性內切酶 |
| 載體大小: | 3666bp |
| 5' 測序引物: | T7 |
| 5' 測序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 載體標簽: | N-T7, C-His |
| 載體抗性: | Ampicillin |
| 克隆菌株: | DH5a |
| 表達菌株: | BL21系列 |
| 備注: |
Same as pET21abcd(+) but no lac; a,b,c,d vary by MCS.
|
| 產品目錄號: | 69745-3 |
| 穩定性: | 穩表達 |
| 組成型: | 組成型 |
| 病毒/非病毒: | 非病毒 |
| 表達宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
| pET表達手冊： |
pET表達手冊英文版 pET表達手冊中文版 |
| pET載體匯總: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
買家導航
pET-23a(+)載體 pET23a質粒基本信息
pET-23a(+)質粒圖譜 pET23a載體圖譜和pET-23a(+)載體序列 pET23a質粒序列多克隆位點信息
pET-23a(+)質粒 pET23a載體簡介
pET-23a-d系列載體帶有N-T7標簽和C-His標簽。pET-23a-d系列載體與pET-21a-d系列載體相比，啟動子由pet21a系列載體的T7lac啟動子，更改為pET-23a-d載體的T7啟動子。獨特的酶切位點在載體圖譜中被標注出來。需要注意的是，這些載體序列是依據載體pBR322的方向來進行編號的，所以T7標簽表達區域在載體圖譜上是反向的。
pET-23a(+)質粒序列 pET23a載體序列
LOCUS pET-23a(+) 3666 bp DNA circular 11-SEP-2008
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
//gt12365.cn/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|gt12365.cn|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
promoter complement(303..319)
/vntifkey="30"
/label=T7\promoter
protein_bind complement(207..239)
/vntifkey="31"
/label=T7\tag
protein_bind complement(140..157)
/vntifkey="31"
/label=His\tag
terminator complement(26..72)
/vntifkey="43"
/label=T7\terminator
rep_origin complement(1450..1450)
/vntifkey="33"
/label=ColE1\pBR322\origin
CDS complement(2211..3068)
/vntifkey="4"
/label=bla\(Ap)\sequence
rep_origin complement(3200..3655)
/vntifkey="33"
/label=f1\origin
BASE COUNT 919 a 936 c 926 g 885 t
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgcgaccc atttgctgtc caccagtcat gctagccata
241 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag agggaaaccg ttgtggtctc
301 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gggcagcgtt gggtcctggc
361 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
421 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
481 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
541 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
601 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
661 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
721 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
781 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg
841 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga
901 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg
961 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
1021 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
1081 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
1141 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
1201 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
1261 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
1321 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
1381 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
1441 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
1501 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
1561 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
1621 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
1681 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
1741 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
1801 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
1861 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
1921 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
1981 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
2041 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
2101 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
2161 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
2221 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
2281 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
2341 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
2401 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
2461 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
2521 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
2581 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
2641 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
2701 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
2761 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
2821 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
2881 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
2941 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
3001 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
3061 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
3121 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
3181 ccccgaaaag tgccacctga aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
3241 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
3301 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
3361 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
3421 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
3481 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
3541 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
3601 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
3661 tcgcca
//
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pET-23a(+)質粒 pET23a載體應用舉例
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