| 出品公司: | ATCC |
|---|---|
| 載體名稱: | YCplac22 |
| 質粒類型: | 釀酒酵母表達載體；中心粒載體 |
| 高拷貝/低拷貝: | 低拷貝 |
| 克隆方法: | 限制性內切酶，多克隆位點 |
| 啟動子: | lacZ |
| 載體大小: | 4854 bp |
| 5' 測序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
| 3' 測序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
| 載體標簽: | -- |
| 載體抗性: | 氨芐青霉素 |
| 篩選標記: | TRP1 |
| 表達菌株: | 釀酒酵母、白色念珠菌 |
| 備注: | -- |
| 產品目錄號: | 87585 |
| 穩定性: | 穩表達 |
| 組成型/誘導型: | 誘導型 |
| 病毒/非病毒: | 非病毒 |
買家導航
YCplac22載體質粒基本信息
YCplac22質粒圖譜載體圖譜和YCplac22載體序列質粒序列多克隆位點信息
YCplac22質粒載體簡介
YCplac22質粒序列載體序列
LOCUS YCplac22 4854 bp DNA SYN
DEFINITION YCplac22
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4854
/organism="YCplac22"
/mol_type="other DNA"
promoter 142..171
/label="lac_promoter"
misc_feature 185..207
/label="M13_pUC_rev_primer"
promoter 206..224
/label="M13_reverse_primer"
misc_feature 287..442
/label="lacZ_a"
promoter complement(290..306)
/label="M13_forward20_primer"
misc_feature complement(299..321)
/label="M13_pUC_fwd_primer"
misc_feature 504..1356
/label="CEN4"
gene complement(2066..2740)
/label="TRP1"
/gene="TRP1"
CDS complement(2066..2740)
/label="ORF frame 3"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK*"
promoter 2983..3011
/label="AmpR_promoter"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK*"
gene 3053..3913
/label="Ampicillin"
/gene="Ampicillin"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
VKNAKK*"
CDS 3053..3913
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 4068..4687
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg
301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
481 gcggtatttc acaccgcata tatcgctggg ccattctcat gaagaatatc ttgaatttat
541 tgtcatatta ctagttggtg tggaagtcca tatatcggtg atcaatatag tggttgacat
601 gctggctagt caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca
661 aatatcaaac ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa
721 gaaaaaaatt actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg
781 tctcaccata gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata
841 catataatac acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa
901 attggctagt gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg
961 taaaagttca ctttagcata catatattac acgagccaga aattgtaact tttgcctaaa
1021 atcacaaatt gcaaaattta attgcttgca aaaggtcaca tgcttataat caactttttt
1081 aaaaatttaa aatacttttt tattttttat ttttaaacat aaatgaaata atttatttat
1141 tgtttatgat taccgaaaca taaaacctgc tcaagaaaaa gaaactgttt tgtccttgga
1201 aaaaaagcac tacctaggag cggccaaaat gccgaggctt tcatagctta aactctttac
1261 agaaaatagg cattatagat cagttcgagt tttcttattc ttccttccgg ttttatcgtc
1321 acagttttac agtaaataag tatcacctct tagagttcga tgataagctg tcaaacatga
1381 gaattaattc cacatgttaa aatagtgaag gagcatgttc ggcacacagt ggaccgaacg
1441 tggggtaagt gcactagggt ccggttaaac ggatctcgca ttgatgaggc aacgctaatt
1501 atcaacatat agattgttat ctatctgcat gaacacgaaa tctttacttg acgacttgag
1561 gctgatggtg tttatgcaaa gaaaccactg tgtttaatat gtgtcactgt ttgatattac
1621 tgtcagcgta gaagataata gtaaaagcgg ttaataagtg tatttgagat aagtgtgata
1681 aagtttttac agcgaaaaga cgataaatac aagaaaatga ttacgaggat acggagagag
1741 gtatgtacat gtgtatttat atactaagct gccggcggtt gtttgcaaga ccgagaaaag
1801 gctagcaaga atcgggtcat tgtagcgtat gcgcctgtga acattctctt caacaagttt
1861 gattccattg cggtgaaatg gtaaaagtca accccctgcg atgtatattt tcctgtacaa
1921 tcaatcaaaa agccaaatga tttagcatta tctttacatc ttgttatttt acagatttta
1981 tgtttagatc ttttatgctt gcttttcaaa aggcttgcag gcaagtgcac aaacaatact
2041 taaataaata ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt
2101 tgttagagtc ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc
2161 catttaatct aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat
2221 gtaagctctc ggggctctct tgccttccaa cccagtcaga aatcgagttc caatccaaaa
2281 gttcacctgt cccacctgct tctgaatcaa acaagggaat aaacgaatga ggtttctgtg
2341 aagctgcact gagtagtatg ttgcagtctt ttggaaatac gagtctttta ataactggca
2401 aaccgaggaa ctcttggtat tcttgccacg actcatctcc atgcagttgg acgatatcaa
2461 tgccgtaatc attgaccaga gccaaaacat cctccttagg ttgattacga aacacgccaa
2521 ccaagtattt cggagtgcct gaactatttt tatatgcttt tacaagactt gaaattttcc
2581 ttgcaataac cgggtcaatt gttctctttc tattgggcac acatataata cccagcaagt
2641 cagcatcgga atctagtgca cattctgcgg cctctgtgct ctgcaagccg caaactttca
2701 ccaatggacc agaactacct gtgaaattaa taacagacat actccaagct gcctttgtgt
2761 gcttaatcac gtatactcac gtgctcaata gtcaccaatg ccctccctct tggccctctc
2821 cttttctttt ttcgaccgaa ttaattcttg aagacgaaag ggcctcgtga tacgcctatt
2881 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
2941 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
3001 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
3061 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
3121 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
3181 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
3241 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
3301 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
3361 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
3421 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
3481 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
3541 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
3601 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
3661 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
3721 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
3781 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
3841 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
3901 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
3961 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
4021 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
4081 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
4141 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
4201 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
4261 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
4321 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
4381 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
4441 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
4501 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
4561 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
4621 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
4681 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
4741 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
4801 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aaga
//