| 質粒名稱: | Flag-HA-USP36 |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 22579 |
| 存儲實驗室: | Wade Harper |
|
載體骨架基本信息 |
|
| 載體名稱: | pDEST_LTR_N_FLAG_HA_IRES_puro |
| 載體出品公司: | Harper_Lab |
| 空載體大小: | 6521bp |
| 完整質粒大小: | 9812bp |
| 載體類型: | 哺乳細胞表達, Retroviral |
| 篩選標記: | Puromycin |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | USP36、ubiquitin specific peptidase 36、DUB1 |
| 物種來源: | H. sapiens (human) |
| 基因突變: | 無 |
| GenBank編號 | NM_025090 |
| 標簽/融合蛋白: |
HA (N terminal on backbone)
FLAG (N terminal on backbone)
|
| 克隆方法: | Gateway Cloning |
| 5’克隆位點: | GATEWAY_LR (unknown if destroyed) |
| 3’克隆位點: | GATEWAY_LR (unknown if destroyed) |
| 5’測序引物 | CAGCCCTCACTCCTTCTCTAGG |
| 3’測序引物 |
CAAGCGGCTTCGGCCAGTAAC
(Common Sequencing Primers)
|
|
文獻引用方法 |
|
| 材料和方法部分: | Flag-HA-USP36 was a gift from Wade Harper (Addgene plasmid # 22579 ; //n2t.net/addgene:22579 ; RRID:Addgene_22579) |
| 參考文獻部分: | Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, Bennett EJ, Gygi SP, Harper JW. Cell. 2009 Jul 23. 138(2):389-403. 10.1016/j.cell.2009.04.042 PubMed 19615732 |
買家導航
Flag-HA-USP36載體質粒基本信息
Flag-HA-USP36質粒圖譜載體圖譜和Flag-HA-USP36載體序列質粒序列多克隆位點信息
Flag-HA-USP36質粒載體簡介
Flag-HA-USP36質粒序列載體序列
LOCUS Flag-HA-USP36 9812 bp DNA circular SYN 14-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9812)
AUTHORS Sowa ME, Bennett EJ, Gygi SP, Harper JW
TITLE Defining the human deubiquitinating enzyme interaction landscape.
JOURNAL Cell. 2009 Jul 23. 138(2):389-403.
PUBMED 19615732
REFERENCE 2 (bases 1 to 9812)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009
Jul 23. 138(2):389-403."
COMMENT Sequence Label: Flag-HA-USP36
FEATURES Location/Qualifiers
source 1..9812
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(63..80)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(234..822)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(314..333)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(993..1784,1785..1853))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1616..1635
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1854..1958)
/gene="bla"
/label=AmpR promoter
primer_bind 2026..2044
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(2082..2104)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 2204..2223
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2417..2439
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2567..2586
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 2786..3302
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
misc_feature 3366..3707
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 3774..4190
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 4120..4142
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 4158..4174
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
regulatory 4203..4212
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 4212..4235
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 4248..4274
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
protein_bind 4290..4314
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(7699..7723)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 7736..8303
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 7753..8305
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(7897..7914)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 8124..8143
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 8317..8916
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(8317..8336)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 8813..8833
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 8992..9506
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 9668..9684
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(9692..9698,9699..9716,9717..9722))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9737..9758
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
1981 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
2041 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
2101 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
2161 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
2221 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
2281 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
2341 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
2401 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat
2461 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
2521 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
2581 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
2641 agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
2701 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
2761 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
2821 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
2881 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
2941 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
3001 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
3061 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
3121 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
3181 gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggac
3241 tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg gggtctttca
3301 tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc cccccgccgg
3361 gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt ctttgtgcgt gtttgtgccg
3421 gcatctaatg tttgcgcctg cgtctgtact agttagctaa ctagctctgt atctggcgga
3481 cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg
3541 actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc
3601 cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
3661 aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca
3721 tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat tagggccaga
3781 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
3841 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
3901 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
3961 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
4021 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
4081 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
4141 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat
4201 ctgccaccat ggactacaag gatgacgatg acaagctcga tggaggatac ccatacgatg
4261 ttccagatta cgctgctcga ggttatcaaa caagtttgta caaaaaagtt ggcgcgccca
4321 ccatgccaat agtggataag ttgaaggagg ccctgaaacc cggccgcaag gactcggctg
4381 atgatggaga actggggaag cttcttgcct cctctgccaa gaaggtcctt ttacagaaaa
4441 tcgagttcga gccagccagc aagagcttct cctaccagct ggaggcctta aagagcaaat
4501 atgtgttgct caaccccaaa acagagggag ctagtcgcca caagagtgga gatgacccac
4561 cggccgggag acagggcagt gagcacacgt atgagagctg tggtgacgga gtcccagccc
4621 cgcagaaagt gcttttcccc acggagcgac tgtctctgag gtgggagcgg gtcttccgcg
4681 tgggcgcagg actccacaac cttggcaaca cctgctttct caatgccacc atccagtgct
4741 tgacctacac accacctcta gccaactacc tgctctccaa ggagcatgct cgcagctgcc
4801 accagggaag cttctgcatg ctgtgtgtca tgcagaacca cattgtccag gccttcgcca
4861 acagcggcaa cgccatcaag cccgtctcct tcatccgaga cctgaaaaag atcgcccgac
4921 acttccgctt tgggaaccag gaggacgcgc atgagttcct gcggtacacc atcgacgcca
4981 tgcagaaagc ctgcctgaat ggctgtgcca agttggatcg tcaaacgcag gctactacct
5041 tggtccatca aatttttgga gggtatctca gatcacgcgt gaagtgctcc gtgtgcaaga
5101 gcgtctcgga cacctacgac ccctacttgg acgtcgcgct ggagatccgg caagctgcga
5161 atattgtgcg tgctctggaa ctttttgtga aagcagatgt cctgagtgga gagaatgcct
5221 acatgtgtgc taaatgcaag aagaaggttc cagccagcaa gcgcttcacc atccacagaa
5281 catccaacgt cttaaccctt tccctcaagc gctttgccaa cttcagcggg gggaagatca
5341 ccaaggatgt aggctatccg gaattcctca acatacgtcc gtatatgtcc cagaataatg
5401 gtgatcctgt catgtatgga ctctatgctg tcctggtgca ctcgggctac agctgccatg
5461 ccgggcacta ttactgctac gtgaaggcaa gcaatggaca gtggtaccag atgaatgatt
5521 ccttggtcca ttccagcaac gtcaaggtgg ttctgaacca gcaggcctac gtgctgttct
5581 atctgcgaat tccaggctct aagaaaagtc ccgagggcct catctccagg acaggctcct
5641 cctcccttcc cggccgcccg agtgtgattc cagatcactc caagaagaac atcggcaatg
5701 ggattatttc ctccccactg actggaaagc gacaagactc tgggacgatg aagaagccgc
5761 acaccactga agagattggt gtgcccatat ccaggaatgg ctccaccctg ggcctgaagt
5821 cccagaacgg ctgcattcct ccaaagctgc cctcggggtc cccttccccc aaactctccc
5881 agacacccac acacatgcca accatcctag acgaccctgg aaagaaggtg aagaagccag
5941 ctcctccaca gcacttttcc cccagaactg ctcaggggct gcctgggacc agcaactcga
6001 atagcagcag atctgggagc caaaggcagg gctcctggga cagcagggat gttgtcctct
6061 ctacctcacc taagctcctg gctacagcca ctgccaacgg gcatgggctg aaggggaacg
6121 acgagagcgc tggcctcgac aggaggggct ccagcagctc cagcccagag cactcggcca
6181 gcagtgactc caccaaggcc ccccagaccc ccaggagtgg agcggcccat ctctgcgatt
6241 ctcaggaaac gaactgttcc accgctggcc actccaaaac gccgccaagt ggagcagatt
6301 ctaagacggt gaagctgaag tcccctgtcc tgagcaacac caccactgag cctgcaagca
6361 ccatgtctcc tccaccagcc aaaaaactgg ccctttctgc caagaaggcc agcaccctgt
6421 ggagggcgac cggcaatgac ctccgtccac ctcccccctc accatcctcc gacctcaccc
6481 accccatgaa aacctctcac cccgtcgttg cctccacttg gcccgtccat agagccaggg
6541 ctgtgtcacc tgctccccaa tcatccagcc gcctgcaacc ccccttcagc ccccacccca
6601 cattgctgtc cagtaccccc aagcccccag ggacgtcaga accacggagc tgctcctcca
6661 tctcgacggc gctgcctcag gtcaacgagg accttgtgtc tcttccacac cagttgccag
6721 aggccagtga gcccccccag agcccctctg agaagaggaa aaagaccttt gtgggagagc
6781 cgcagaggct gggctcagag acgcgcctcc cacagcacat cagggaggcc actgcggctc
6841 cccacgggaa gaggaagagg aagaagaaga agcgcccgga ggacacagct gccagcgccc
6901 tgcaggaggg gcagacacag agacagcctg ggagccccat gtacaggagg gagggccagg
6961 cacagctgcc cgctgtcaga cggcaggaag atggcacaca gccacaggtg aatggccagc
7021 aggtgggatg tgttacggac ggccaccacg cgagcagcag gaagcggagg aggaaaggag
7081 cagaaggtct tggtgaagaa ggcggcctgc accaggaccc acttcggcac agctgctctc
7141 ccatgggtga tggtgatcca gaggccatgg aagagtctcc aaggaaaaag aaaaagaaaa
7201 aaagaaagca ggagacacag cgggcagtag aagaggatgg gcatctcaaa tgcccaagga
7261 gtgccaagcc ccaagatgct gttgtccccg agtccagcag ctgcgcacca tccgcgaatg
7321 gctggtgtcc tggggaccgc atggggctga gccaggcccc tcctgtgtct tggaatggag
7381 agcgggagtc tgatgtggtc caggaactgc tcaaatactc atctgataaa gcttacggga
7441 gaaaagttct gacctgggat ggcaagatgt cggcggtcag tcaggatgct attgaagaca
7501 gcagacaggc ccggactgag accgtggttg atgactggga cgaagagttt gaccgaggga
7561 aggaaaagaa aattaaaaaa tttaagagag agaagaggag aaacttcaac gccttccaga
7621 aacttcagac tcgacggaac ttctggtctg tgactcaccc agcaaaggct gccagcctca
7681 gctatcgccg cttaattaat ccaactttct tgtacaaagt ggttcgataa cgaattccgc
7741 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
7801 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
7861 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
7921 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
7981 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
8041 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
8101 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
8161 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
8221 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
8281 tcctttgaaa aacacgatga taatatggcc acaaccatga ccgagtacaa gcccacggtg
8341 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
8401 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
8461 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
8521 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
8581 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
8641 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
8701 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
8761 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
8821 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
8881 cgcacctggt gcatgacccg caagcccggt gcctgatgca tcgatgtcga cctgcagcca
8941 agcttatcga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
9001 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaata
9061 cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg
9121 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
9181 tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
9241 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
9301 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
9361 gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtgtatccaa taaaccctct
9421 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
9481 actacccgtc agcgggggtc tttcatgggt aacagtttct tgaagttgga gaacaacatt
9541 ctgagggtag gagtcgaata ttaagtaatc ctgactcaat tagccactgt tttgaatcca
9601 catactccaa tactcctgaa atagttcatt atggacagcg cagaaagagc tggggagaat
9661 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
9721 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
9781 ctttccagtc gggaaacctg tcgtgccagc tg
//
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