| 質粒名稱: | pAAVscCBPIGpluc |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 34645 |
| 存儲實驗室: | Phillip Zamore |
|
載體骨架基本信息 |
|
| 載體名稱: | pN1 |
| 載體出品公司: | pAAVscCBPIGpluc |
| 空載體大小: | 4734bp |
| 完整質粒大小: | 4734bp |
| 載體類型: | AAV |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷貝數: | 高拷貝 |
|
文獻引用方法 |
|
| 材料和方法部分: | pAAVscCBPIGpluc was a gift from Phillip Zamore (Addgene plasmid # 35645 ; //n2t.net/addgene:35645 ; RRID:Addgene_35645) |
| 參考文獻部分: | Long-term, efficient inhibition of microRNA function in mice using rAAV vectors. Xie J, Ameres SL, Friedline R, Hung JH, Zhang Y, Xie Q, Zhong L, Su Q, He R, Li M, Li H, Mu X, Zhang H, Broderick JA, Kim JK, Weng Z, Flotte TR, Zamore PD, Gao G. Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903. 10.1038/nmeth.1903 PubMed 22388288 |
買家導航
pAAVscCBPIGpluc載體質粒基本信息
pAAVscCBPIGpluc質粒圖譜載體圖譜和pAAVscCBPIGpluc載體序列質粒序列多克隆位點信息
pAAVscCBPIGpluc質粒載體簡介
pAAVscCBPIGpluc質粒序列載體序列
LOCUS pAAVscCBPIGpluc 4734 bp DNA circular SYN 14-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4734)
AUTHORS Xie J, Ameres SL, Friedline R, Hung JH, Zhang Y, Xie Q, Zhong L, Su
Q, He R, Li M, Li H, Mu X, Zhang H, Broderick JA, Kim JK, Weng Z,
Flotte TR, Zamore PD, Gao G
TITLE Long-term, efficient inhibition of microRNA function in mice using
rAAV vectors.
JOURNAL Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903.
PUBMED 22388288
REFERENCE 2 (bases 1 to 4734)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth";
journalName: "Nat Methods"; date: "2012-03-4- 4"; volume: "9";
issue: "4"; pages: "403-9"
COMMENT Sequence Label: pAAVscCBPIGpluc
FEATURES Location/Qualifiers
source 1..4734
/mol_type="other DNA"
/organism="synthetic DNA construct"
intron 797..893
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
CDS 974..1531
/codon_start=1
/product="secreted Gaussia luciferase"
/label=hGLuc
/note="human codon-optimized"
/translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADRG
KLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKESA
QGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKWLPQ
RCATFASKIQGQVDKIKGAGGD"
primer_bind complement(1547..1564)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 1553..1664
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 1784..1913
/label=AAV2 ITR
primer_bind complement(1932..1949)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(1932..1948)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1941..1963)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 2090..2545
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2177..2196)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2387..2408
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 2571..2675
/gene="bla"
/label=AmpR promoter
CDS join(2676..2744,2745..3536)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2894..2913)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3707..4295
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4196..4215
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
protein_bind 4583..4604
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(4619..4624,4625..4642,4643..4649)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4657..4673
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4662..4684
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 4681..4697
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4681..4697
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
ORIGIN
1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtgtagcc atgctctagg
121 aagatcaatt cggtacaatt cacgcgtcga cattgattat tgactctggt cgttacataa
181 cttacggtaa atggcccgcc tggctgaccg cccaacgacc ccgcccattg acgtcaataa
241 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
301 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
361 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
421 gggactttcc tacttggcag tacatctact cgaggccacg ttctgcttca ctctccccat
481 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
541 gatgggggcg gggggggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
601 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
661 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
721 gggcgggagc gggatcagcc accgcggtgg cggccctaga gtcgatcgag gaactgaaaa
781 accagaaagt taactggtaa gtttagtctt tttgtctttt atttcaggtc ccggatccgg
841 tggtggtgca aatcaaagaa ctgctcctca gtggatgttg cctttacttc taggcctgta
901 cggaagtgtt acttctgctc taaaagctgc ggaattgtac ccgcggccga tccaccggtc
961 gccaccatct agcatgggag tcaaagttct gtttgccctg atctgcatcg ctgtggccga
1021 ggccaagccc accgagaaca acgaagactt caacatcgtg gccgtggcca gcaacttcgc
1081 gaccacggat ctcgatgctg accgcgggaa gttgcccggc aagaagctgc cgctggaggt
1141 gctcaaagag atggaagcca atgcccggaa agctggctgc accaggggct gtctgatctg
1201 cctgtcccac atcaagtgca cgcccaagat gaagaagttc atcccaggac gctgccacac
1261 ctacgaaggc gacaaagagt ccgcacaggg cggcataggc gaggcgatcg tcgacattcc
1321 tgagattcct gggttcaagg acttggagcc catggagcag ttcatcgcac aggtcgatct
1381 gtgtgtggac tgcacaactg gctgcctcaa agggcttgcc aacgtgcagt gttctgacct
1441 gctcaagaag tggctgccgc aacgctgtgc gacctttgcc agcaagatcc agggccaggt
1501 ggacaagatc aagggggccg gtggtgacta gctcgacgct gatcagcctc gactgtgcct
1561 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
1621 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
1681 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
1741 aattaggtag ataagtagca tggcgggtta atcattaact acaaggaacc cctagtgatg
1801 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
1861 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagccttaat
1921 taacctaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
1981 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
2041 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
2101 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
2161 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
2221 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
2281 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
2341 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
2401 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
2461 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
2521 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
2581 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
2641 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
2701 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
2761 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
2821 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
2881 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
2941 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
3001 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
3061 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
3121 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
3181 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
3241 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
3301 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
3361 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
3421 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
3481 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
3541 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
3601 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
3661 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
3721 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
3781 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
3841 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
3901 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
3961 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
4021 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
4081 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
4141 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
4201 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
4261 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
4321 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
4381 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
4441 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
4501 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
4561 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
4621 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
4681 caggaaacag ctatgaccat gattacgcca gatttaatta aggccttaat tagg
//