| 質粒名稱: | pHBV-X/EnhI-Luc |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 71418 |
| 存儲實驗室: | Wang-Shick Ryu |
|
載體骨架基本信息 |
|
| 載體名稱: | pGL3-Basic |
| 載體出品公司: | Promega |
| 空載體大小: | 3100bp |
| 完整質粒大小: | 5200 bp |
| 載體類型: | 哺乳細胞表達, Luciferase |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 未知 |
|
插入基因信息 |
|
| 插入基因名稱: | HBV X promoter and enhancer I driving Luciferase expression |
| 物種來源: | hepatitis B virus |
| 啟動子: | HBV X |
| 克隆方法: | 未知 |
| 5’克隆位點: | AgeI(未破壞) |
| 3’克隆位點: | NotI (未破壞) |
| 5’測序引物 | RVprimer3 |
| 3’測序引物 |
LucNRev
(Common Sequencing Primers)
|
|
文獻引用方法 |
|
| 材料和方法部分: | pHBV-X/EnhI-Luc was a gift from Wang-Shick Ryu (Addgene plasmid # 71418 ; //n2t.net/addgene:71418 ; RRID:Addgene_71418) |
| 參考文獻部分: | DDX3 DEAD-box RNA helicase is a host factor that restricts hepatitis B virus replication at the transcriptional level. Ko C, Lee S, Windisch MP, Ryu WS. J Virol. 2014 Dec;88(23):13689-98. doi: 10.1128/JVI.02035-14. Epub 2014 Sep 17. 10.1128/JVI.02035-14 PubMed 25231298 |
買家導航
pHBV-X/EnhI-Luc載體質粒基本信息
pHBV-X/EnhI-Luc質粒圖譜載體圖譜和pHBV-X/EnhI-Luc載體序列質粒序列多克隆位點信息
pHBV-X/EnhI-Luc質粒載體簡介
pHBV-X/EnhI-Luc質粒序列載體序列
LOCUS pHBV-X/EnhI-Luc 5204 bp DNA circular SYN 14-APR-2025
DEFINITION To see HBV X promoter(overlaped EnhanceI) activity for luciferase
assay.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5204)
AUTHORS Ko C, Lee S, Windisch MP, Ryu WS
TITLE DDX3 DEAD-box RNA helicase is a host factor that restricts hepatitis
B virus replication at the transcriptional level.
JOURNAL J Virol. 2014 Dec;88(23):13689-98. doi: 10.1128/JVI.02035-14. Epub
2014 Sep 17.
PUBMED 25231298
REFERENCE 2 (bases 1 to 5204)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1128/JVI.02035-14"; journalName: "J Virol"; date: "2014-12";
volume: "88"; issue: "23"; pages: "13689-98"
COMMENT Sequence Label: pHBV-X/EnhI-Luc
FEATURES Location/Qualifiers
source 1..5204
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 468..477
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 474..2126
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(540..558)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 2167..2288
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2178..2197)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2232..2251
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2536..2553)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2707..3295)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2787..2806)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(3466..4257,4258..4326))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4089..4108
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4327..4431)
/gene="bla"
/label=AmpR promoter
rep_origin 4458..4913
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4545..4564)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4755..4776
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 5044..5092
/note="synthetic polyadenylation signal"
misc_feature 5106..5197
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 5146..5165
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagtag aaaacttcct attaacaggc
61 ctattgattg gaaagtatgt caacgaattg tgggtctttt gggttttgct gcccctttta
121 cacaatgtgg ttatcctgcg ttgatgcctt tgtatgcatg tattcaatct aagcaggctt
181 tcactttctc gccaacttac aaggcctttc tgtgtaaaca atacctgaac ctttaccccg
241 ttgcccggca acggccaggt ctgtgccaag tgtttgctga cgcaaccccc actggctggg
301 gcttggtcat gggccatcag cgcatgcgtg gaaccttttc ggctcctctg ccgatccata
361 ctgcggaact cctagccgct tgttttgctc gcagcaggtc tggagcaaac attatcggga
421 ctgataactc tgttgtccaa gcttggcatt ccggtactgt tggtaaagcc accatggaag
481 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg
541 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
601 cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt
661 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
721 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
781 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc
841 ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc
901 tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt
961 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc
1021 cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg
1081 gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca
1141 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat
1201 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg
1261 tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc
1321 aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca
1381 aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag
1441 tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca
1501 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg
1561 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg
1621 gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa
1681 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca
1741 tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta
1801 agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca
1861 acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg
1921 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg
1981 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
2041 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
2101 agaagggcgg aaagatcgcc gtgtaattct agagtcgggg cggccggccg cttcgagcag
2161 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
2221 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
2281 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
2341 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga
2401 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
2461 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
2521 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
2581 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
2641 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
2701 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
2761 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
2821 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
2881 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
2941 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
3001 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
3061 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
3121 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
3181 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
3241 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
3301 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
3361 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
3421 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
3481 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
3541 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
3601 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
3661 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
3721 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
3781 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
3841 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
3901 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
3961 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
4021 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
4081 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
4141 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
4201 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
4261 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
4321 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
4381 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
4441 ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
4501 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
4561 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
4621 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
4681 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
4741 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
4801 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
4861 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttgccattc
4921 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
4981 ccagcccaag ctaccatgat aagtaagtaa tattaaggta cgggaggtac ttggagcggc
5041 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata
5101 gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct
5161 gtccccagtg caagtgcagg tgccagaaca tttctctatc gata
//