| 質粒名稱: | pLJC5-Tmem192-3xHA |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 102930 |
| 存儲實驗室: | David Sabatini |
|
載體骨架基本信息 |
|
| 載體名稱: | pLJC5 |
| 載體出品公司: | Clontech |
| 空載體大小: | 4038 bp |
| 完整質粒大小: | 8954bp |
| 載體類型: | 哺乳細胞表達 |
| 篩選標記: | Puromycin |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷貝數: | 未知 |
|
插入基因信息 |
|
| 插入基因名稱: | Tmem192 |
| 物種來源: | H. sapiens (human) |
| 啟動子: | ubc |
| 標簽/融合蛋白: | HA (C terminal on insert) |
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | AgeI (not destroyed) |
| 3’克隆位點: | EcorI (未破壞) |
| 5’測序引物 | CGAAGGAATAGAAGAAGAAGGTGGAGA |
| 3’測序引物 | SV40pA-R |
|
文獻引用方法 |
|
| 材料和方法部分: | pLJC5-Tmem192-3xHA was a gift from David Sabatini (Addgene plasmid # 102930 ; //n2t.net/addgene:102930 ; RRID:Addgene_102930) |
| 參考文獻部分: | Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent regulation of amino acid efflux from lysosomes. Abu-Remaileh M, Wyant GA, Kim C, Laqtom NN, Abbasi M, Chan SH, Freinkman E, Sabatini DM. Science. 2017 Nov 10;358(6364):807-813. doi: 10.1126/science.aan6298. Epub 2017 Oct 26. 10.1126/science.aan6298 PubMed 29074583 |
買家導航
pLJC5-Tmem192-3xHA載體質粒基本信息
pLJC5-Tmem192-3xHA質粒圖譜載體圖譜和 pLJC5-Tmem192-3xHA載體序列質粒序列多克隆位點信息
pLJC5-Tmem192-3xHA質粒載體簡介
pLJC5-Tmem192-3xHA質粒序列載體序列
LOCUS pLJC5-Tmem192-3xHA 8954 bp DNA circular SYN 14-APR-2025
DEFINITION Express a lysosomal Tag.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8954)
AUTHORS Abu-Remaileh M, Wyant GA, Kim C, Laqtom NN, Abbasi M, Chan SH,
Freinkman E, Sabatini DM
TITLE Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent
regulation of amino acid efflux from lysosomes.
JOURNAL Science. 2017 Nov 10;358(6364):807-813. doi:
10.1126/science.aan6298. Epub 2017 Oct 26.
PUBMED 29074583
REFERENCE 2 (bases 1 to 8954)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1126/science.aan6298"; journalName: "Science"; date:
"2017-11-10- 10"; volume: "358"; issue: "6364"; pages: "807-813"
COMMENT Sequence Label: pLJC5-Tmem192-3xHA
FEATURES Location/Qualifiers
source 1..8954
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 259..278
/label=hUBCpro-F
/note="Human Ubiquitin C (UbC) promoter, forward primer"
regulatory 444..453
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1274..1300
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 1313..1339
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 1352..1378
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
misc_feature 1435..1552
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 1601..2111
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 2030..2048
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 2133..2732
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2133..2152)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2629..2649
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 2860..3093
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 3165..3286
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3202..3221)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3256..3275
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 3326..3461
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(3481..3500)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(3482..3500)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3510..3527)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(3510..3526)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3519..3541)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 3668..4123
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3755..3774)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3965..3986
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 4150..4253
/gene="bla"
/label=AmpR promoter
CDS join(4254..4322,4323..5114)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4472..4491)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5285..5873
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5774..5793
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6027..6044
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 6161..6182
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(6197..6202,6203..6220,6221..6227)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6235..6251
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6240..6262
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 6259..6275
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6259..6275
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 6294..6314
/label=T3
/note="T3 promoter, forward primer"
promoter 6296..6314
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 6342..6568
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 6569..6749
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 6796..6921
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 7414..7647
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 7832..7876
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter join(8165..8954,1..422)
/label=UbC promoter
/note="human ubiquitin C promoter"
ORIGIN
1 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
61 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
121 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
181 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
241 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
301 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
361 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
421 acgaagcgct agcgctaccg gtaccatggc ggcggggggc aggatggagg acggttcctt
481 ggatatcacc cagagtattg aagacgaccc acttctggat gcccagcttc tcccacacca
541 ctcattacaa gctcacttta gaccccgatt ccatcctctt cctacagtca tcatagtgaa
601 tcttctgtgg tttattcatc tcgtgtttgt tgttttagca tttttaacag gtgtgctttg
661 ttcttatcct aatccaaatg aggacaagtg cccaggaaat tacacaaacc cattgaaagt
721 tcagacggtt ataatccttg ggaaagttat tttgtggatt ctccatttac tccttgaatg
781 ctacatccag tatcaccaca gcaaaatcag aaaccgaggc tataacttga tctaccgatc
841 aacaaggcat ctcaagagac ttgcgttgat gatacagtcc tctggcaaca cagtgcttct
901 cctcatactg tgcatgcagc actccttccc agagcctggc agattgtatc ttgacctcat
961 tctggccatc ttggcactgg aactcatctg ttccctgata tgtctcctca tttacacagt
1021 gaaaatccgg agatttaata aagctaaacc agagcctgat atacttgaag aagaaaaaat
1081 ctatgcttac cccagcaata ttacctcgga gactggattc agaactattt caagcctaga
1141 agaaattgtt gaaaagcaag gagacaccat tgaatacctg aagcgacaca atgcgctgct
1201 gagtaagcga ttgttggctc tcacttcctc agacctgggc tgtcagccaa gtagaacggg
1261 aggtggaggt agttatccct atgacgtgcc tgattacgcc ggcacaggat cctaccccta
1321 tgatgtgcct gactacgctg gcagcgccgg atacccttat gatgtgcctg attatgctgg
1381 agggagcggc taagaattct cgacctcgag acaaatggca gtattcatcc acaattttaa
1441 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
1501 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
1561 ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt
1621 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac
1681 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga
1741 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg
1801 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta
1861 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
1921 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
1981 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
2041 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
2101 tctctcccca gggggatcca ccggagctta ccatgaccga gtacaagccc acggtgcgcc
2161 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
2221 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
2281 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
2341 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
2401 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
2461 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
2521 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
2581 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
2641 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
2701 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
2761 ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact tacaaggcag
2821 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
2881 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
2941 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
3001 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
3061 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
3121 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
3181 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
3241 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
3301 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
3361 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
3421 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
3481 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
3541 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
3601 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
3661 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
3721 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
3781 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
3841 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
3901 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
3961 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
4021 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
4081 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
4141 gaaatgtgtg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
4201 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
4261 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
4321 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
4381 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
4441 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
4501 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
4561 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
4621 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
4681 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
4741 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
4801 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
4861 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
4921 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
4981 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
5041 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
5101 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
5161 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
5221 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
5281 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
5341 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
5401 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
5461 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
5521 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
5581 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
5641 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
5701 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
5761 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
5821 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
5881 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
5941 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
6001 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
6061 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
6121 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
6181 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
6241 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
6301 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
6361 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
6421 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
6481 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
6541 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
6601 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
6661 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
6721 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
6781 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
6841 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
6901 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
6961 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
7021 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
7081 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
7141 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
7201 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagca
7261 gcagctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
7321 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
7381 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
7441 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
7501 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
7561 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
7621 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
7681 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
7741 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
7801 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
7861 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
7921 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
7981 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
8041 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
8101 atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc ctcgagcggg
8161 atctggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc
8221 gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg
8281 acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt
8341 ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc
8401 gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc
8461 cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt
8521 gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg
8581 ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga
8641 ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg
8701 agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct
8761 gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg
8821 ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga
8881 ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg
8941 cagttatggc ggtg
//