LOCUS       pCas-Scramble-EF1a-GFP              10469 bp DNA     circular SYN 06-JUN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10469)
  AUTHORS   Ming
  TITLE     Direct Submission
  JOURNAL   Exported Jun 6, 2025 from SnapGene 6.0.2
            //gt12365.cn
FEATURES             Location/Qualifiers
     source          1..10469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        90..330
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        360..435
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        456..1634
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          687..1625
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     CDS             1713..2408
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     protein_bind    complement(2920..2953)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     enhancer        3023..3326
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3327..3530
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        3556..3574
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3633..7736
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7737..7757
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7764..7784
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7806..7835
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7854..7877
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7897..7913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7924..8546
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     rep_origin      complement(8667..9255)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9430..10290)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10405..10421
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10426..10444
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaattcccca gtggaaagac gcgcaggcaa aacgcaccac gtgacggagc gtgaccgcgc
       61 gccgagcgcg cgccaaggtc gggcaggaag agggcctatt tcccatgatt ccttcatatt
      121 tgcatatacg atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa
      181 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
      241 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
      301 tcttgggttt atatatcttg tggaaaggac gcgggatcgg cactaccaga gctaactcag
      361 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
      421 gcaccgagtc ggtgcttttt ttggtgtaca cgtgaggctc cggtgcccgt cagtgggcag
      481 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      541 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      601 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      661 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      721 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      781 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      841 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      901 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      961 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     1021 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     1081 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     1141 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     1201 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     1261 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     1321 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1381 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1441 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1501 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1561 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1621 ttcaggtgtc gtgactatag ggcggccgga cgtgacaaat ggaagtagca cgcctcacta
     1681 ggctcgtgca gatggacagc accgctgcag ccatggagag cgacgagagc ggcctgcccg
     1741 ccatggagat cgagtgccgc atcaccggca ccctgaacgg cgtggagttc gagctggtgg
     1801 gcggcggaga gggcaccccc gagcagggcc gcatgaccaa caagatgaag agcaccaaag
     1861 gcgccctgac cttcagcccc tacctgctga gccacgtgat gggctacggc ttctaccact
     1921 tcggcaccta ccccagcggc tacgagaacc ccttcctgca cgccatcaac aacggcggct
     1981 acaccaacac ccgcatcgag aagtacgagg acggcggcgt gctgcacgtg agcttcagct
     2041 accgctacga ggccggccgc gtgatcggcg acttcaaggt gatgggcacc ggcttccccg
     2101 aggacagcgt gatcttcacc gacaagatca tccgcagcaa cgccaccgtg gagcacctgc
     2161 accccatggg cgataacgat ctggatggca gcttcacccg caccttcagc ctgcgcgacg
     2221 gcggctacta cagctccgtg gtggacagcc acatgcactt caagagcgcc atccacccca
     2281 gcatcctgca gaacgggggc cccatgttcg ccttccgccg cgtggaggag gatcacagca
     2341 acaccgagct gggcatcgtg gagtaccagc acgccttcaa gaccccggat gcagatgccg
     2401 gtgaagaaag agtttaatcg atgatatcag atccccggga tgcagaaatt gatgatctat
     2461 taaacaataa agatgtccac taaaatggaa gtttttcctg tcatactttg ttaagaaggg
     2521 tgagaacaga gtacctacat tttgaatgga aggattggag ctacgggggt gggggtgggg
     2581 tgggattaga taaatgcctg ctctttactg aaggctcttt actattgctt tatgataatg
     2641 tttcatagtt ggatatcata atttaaacaa gcaaaaccaa attaagggcc agctcattcc
     2701 tcccactcat gatctataga tctatagatc tctcgtggga tcattgtttt tctcttgatt
     2761 cccactttgt ggttctaagt actgtggttt ccaaatgtgt cagtttcata gcctgaagaa
     2821 cgagatcagc agcctctgtt ccacatacac ttcattctca gtattgtttt gccaagttct
     2881 aattccatca gaagctggtc gagatccgga acccttaata taacttcgta taatgtatgc
     2941 tatacgaagt tattaggtcc actagttatt aatagtaatc aattacgggg tcattagttc
     3001 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     3061 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     3121 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     3181 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
     3241 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
     3301 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
     3361 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     3421 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
     3481 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
     3541 accgtcagaa ttttgtaata cgactcacta tagggcggcc gggaattcgt cgactggaac
     3601 cggtaccgag gagatctgcc gccgcgatcg ccatggataa gaaatactca ataggactgg
     3661 atattggcac aaatagcgtc ggatgggctg tgatcactga tgaatataag gttccttcta
     3721 aaaagttcaa ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg
     3781 ctcttctgtt tgacagtgga gagacagccg aagctactag actcaaacgg acagctagga
     3841 gaaggtatac aagacggaag aataggattt gttatctcca ggagattttt tcaaatgaga
     3901 tggccaaagt ggatgatagt ttctttcata gacttgaaga gtcttttttg gtggaagaag
     3961 acaagaagca tgaaagacat cctatttttg gaaatatagt ggatgaagtt gcttatcacg
     4021 agaaatatcc aactatctat catctgagaa aaaaattggt ggattctact gataaagccg
     4081 atttgcgcct gatctatttg gccctggccc acatgattaa gtttagaggt cattttttga
     4141 ttgagggcga tctgaatcct gataatagtg atgtggacaa actgtttatc cagttggtgc
     4201 aaacctacaa tcaactgttt gaagaaaacc ctattaacgc aagtggagtg gatgctaaag
     4261 ccattctttc tgcaagattg agtaaatcaa gaagactgga aaatctcatt gctcagctcc
     4321 ccggtgagaa gaaaaatggc ctgtttggga atctcattgc tttgtcattg ggtttgaccc
     4381 ctaattttaa atcaaatttt gatttggcag aagatgctaa actccagctt tcaaaagata
     4441 cttacgatga tgatctggat aatctgttgg ctcaaattgg agatcaatat gctgatttgt
     4501 ttttggcagc taagaatctg tcagatgcta ttctgctttc agacatcctg agagtgaata
     4561 ctgaaataac taaggctccc ctgtcagctt caatgattaa acgctacgat gaacatcatc
     4621 aagacttgac tcttctgaaa gccctggtta gacaacaact tccagaaaag tataaagaaa
     4681 tcttttttga tcaatcaaaa aacggatatg caggttatat tgatggcggc gcaagccaag
     4741 aagaatttta taaatttatc aaaccaattc tggaaaaaat ggatggtact gaggaactgt
     4801 tggtgaaact gaatagagaa gatttgctgc gcaagcaacg gacctttgac aacggctcta
     4861 ttccccatca aattcacttg ggtgagctgc atgctatttt gagaagacaa gaagactttt
     4921 atccatttct gaaagacaat agagagaaga ttgaaaaaat cttgactttt aggattcctt
     4981 attatgttgg tccattggcc agaggcaata gtaggtttgc atggatgact cggaagtctg
     5041 aagaaacaat taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat
     5101 catttattga acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtgctgccaa
     5161 aacatagttt gctttatgag tattttaccg tttataacga attgacaaag gtcaaatatg
     5221 ttactgaagg aatgagaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg
     5281 atctgctctt caaaacaaat aggaaagtga ccgttaagca actgaaagaa gattatttca
     5341 aaaaaataga atgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt
     5401 cactgggtac ataccatgat ttgctgaaaa ttattaaaga taaagatttt ttggataatg
     5461 aagaaaatga agacatcctg gaggatattg ttctgacatt gaccctgttt gaagataggg
     5521 agatgattga ggaaagactt aaaacatacg ctcacctctt tgatgataag gtgatgaaac
     5581 agcttaaaag acgcagatat actggttggg gaaggttgtc cagaaaattg attaatggta
     5641 ttagggataa gcaatctggc aaaacaatac tggatttttt gaaatcagat ggttttgcca
     5701 atcgcaattt tatgcagctc atccatgatg atagtttgac atttaaagaa gacatccaaa
     5761 aagcacaagt gtctggacaa ggcgatagtc tgcatgaaca tattgcaaat ctggctggta
     5821 gccctgctat taaaaaaggt attctccaga ctgtgaaagt tgttgatgaa ttggtcaaag
     5881 tgatggggcg gcataagcca gaaaatatcg ttattgaaat ggcaagagaa aatcagacaa
     5941 ctcaaaaggg ccagaaaaat tccagagaga ggatgaaaag aatcgaagaa ggtatcaaag
     6001 aactgggaag tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa
     6061 agctctatct ctattatctc caaaatggaa gagatatgta tgtggaccaa gaactggata
     6121 ttaataggct gagtgattat gatgtcgatc acattgttcc acaaagtttc cttaaagacg
     6181 attcaataga caataaggtc ctgaccaggt ctgataaaaa tagaggtaaa tccgataacg
     6241 ttccaagtga agaagtggtc aaaaagatga aaaactattg gagacaactt ctgaacgcca
     6301 agctgatcac tcaaaggaag tttgataatc tgaccaaagc tgaaagagga ggtttgagtg
     6361 aacttgataa agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc
     6421 atgtggcaca aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta
     6481 ttagagaggt taaagtgatt accctgaaat ctaaactggt ttctgacttc agaaaagatt
     6541 tccaattcta taaagtgaga gagattaaca attaccatca tgcccatgat gcctatctga
     6601 atgccgtcgt tggaactgct ttgattaaga aatatccaaa acttgaaagc gagtttgtct
     6661 atggtgatta taaagtttat gatgttagga aaatgattgc taagtctgag caagaaatag
     6721 gcaaagcaac cgcaaagtat ttcttttact ctaatatcat gaacttcttc aaaacagaaa
     6781 ttacacttgc aaatggagag attcgcaaac gccctctgat cgaaactaat ggggaaactg
     6841 gagaaattgt ctgggataaa gggagagatt ttgccacagt gcgcaaagtg ttgtccatgc
     6901 cccaagtcaa tatcgtcaag aaaacagaag tgcagacagg cggattctct aaggagtcaa
     6961 ttctgccaaa aagaaattcc gacaagctga ttgctaggaa aaaagactgg gacccaaaaa
     7021 aatatggtgg ttttgatagt ccaaccgtgg cttattcagt cctggtggtt gctaaggtgg
     7081 aaaaagggaa atccaagaag ctgaaatccg ttaaagagct gctggggatc acaattatgg
     7141 aaagaagttc ctttgaaaaa aatcccattg actttctgga agctaaagga tataaggaag
     7201 ttaaaaaaga cctgatcatt aaactgccta aatatagtct ttttgagctg gaaaacggta
     7261 ggaaacggat gctggctagt gccggagaac tgcaaaaagg aaatgagctg gctctgccaa
     7321 gcaaatatgt gaattttctg tatctggcta gtcattatga aaagttgaag ggtagtccag
     7381 aagataacga acaaaaacaa ttgtttgtgg agcagcataa gcattatctg gatgagatta
     7441 ttgagcaaat cagtgaattt tctaagagag ttattctggc agatgccaat ctggataaag
     7501 ttcttagtgc atataacaaa catagagaca aaccaataag agaacaagca gaaaatatca
     7561 ttcatctgtt taccttgacc aatcttggag cacccgctgc ttttaaatac tttgatacaa
     7621 caattgatag gaaaagatat acctctacaa aagaagttct ggatgccact cttatccatc
     7681 aatccatcac tggtctttat gaaacacgca ttgatttgag tcagctggga ggtgacccca
     7741 agaaaaaacg caaggtggaa gatcctaaga aaaagcggaa agtggacacg cgtacgcggc
     7801 cgctcgagca gaaactcatc tcagaagagg atctggcagc aaatgatatc ctggattaca
     7861 aggatgacga cgataaggtt taaacggccg gccgcggtca tagctgtttc ctgaacagat
     7921 cccgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
     7981 ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
     8041 tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
     8101 acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct
     8161 tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
     8221 ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagagg
     8281 cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
     8341 ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
     8401 ctgattttaa aataactata ccagcaggag gacgtccaga cacagcatag gctacctggc
     8461 catgcccaac cggtgggaca tttgagttgc ttgcttggca ctgtcctctc atgcgttggg
     8521 tccactcagt agatgcctgt tgaattgggt acgcggccag cggcgagcgg tatcagctca
     8581 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtc
     8641 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8701 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8761 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8821 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8881 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8941 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9001 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9061 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9121 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9181 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9241 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9301 gacgcgtaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9361 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9421 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9481 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9541 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9601 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9661 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9721 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
     9781 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9841 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9901 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9961 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10021 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10081 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10141 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10201 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10261 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10321 ttatcagggt tattgtctca tgatgatatt attttatctt gtgcaatgta acatcagaga
    10381 ttttgagaca cgggccagag ctgccaggaa acagctatga ccatgtaata cgactcacta
    10441 taggggatat cagctggatg gcagttaac
//