| 出品公司: | Agilent |
|---|---|
| 載體名稱: | pAAVS1-puro-DNR |
| 質粒類型: | 哺乳動物細胞載體；逆病毒包裝載體 |
| 高拷貝/低拷貝: | 高拷貝 |
| 克隆方法: | 限制性內切酶，多克隆位點 |
| 啟動子: | CMV |
| 載體大小: | 6.8kb |
| 5’測序引物: | CMV-F:CGCAAATGGGCGGTAGGCGTG (Invitrogen) |
| 3’測序引物: | -- |
| 載體標簽: | C-Myc，C-DDK |
| 載體抗性: | 氨芐青霉素 |
| 篩選標記: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | DH5 alpha; 等 |
| 宿主細胞（系）: | 常規細胞系（293、CV-1、CHO等） |
| 產品目錄號: | GE100024 |
| 穩定性: | 瞬表達 |
| 組成型/誘導型: | 組成型 |
| 病毒/非病毒: | 病毒 |
| 備注: |
買家導航
pAAVS1-puro-DNR載體質粒基本信息
pAAVS1-puro-DNR質粒圖譜載體圖譜和pAAVS1-puro-DNR載體序列質粒序列多克隆位點信息
pAAVS1-puro-DNR質粒載體簡介
pAAVS1-puro-DNR質粒序列載體序列
LOCUS pAAVS1-Puro-DNR 7507 bp DNA circular SYN 26-OCT-2024
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7507)
AUTHORS Ming
TITLE Direct Submission
JOURNAL Exported Oct 26, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
//gt12365.cn
COMMENT VNTDATE|1072224000|
COMMENT VNTDBDATE|1072224000|
COMMENT VNTNAME|pAAVS1-Puro-DNR|
COMMENT VNTAUTHORNAME|Ming|
FEATURES Location/Qualifiers
misc_feature 7..536
/label=HA-R
/note="right homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
polyA_signal 611..1087
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind 1098..1114
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(1134..1157)
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
CDS complement(1176..1205)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
promoter complement(1320..1338)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(1364..1567)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer 1568..1871
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1989..2488
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 2528..3127
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
misc_feature 3729..4230
/label=HA-L
/note="left homology arm from the adeno-associated virus
integration site (AAVS1) within intron 1 of the human
PPP1R12C gene"
primer_bind complement(4321..4337)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 4550..5005
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5287..5391
/gene="bla"
/label=AmpR promoter
CDS 5392..6252
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
rep_origin 6423..7011
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 7299..7320
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7335..7365
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7373..7389
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7397..7413
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 tctagacaga gcagggcctt agggaagcgg gaccctgctc tgggcggagg aatatgtccc
61 agatagcact ggggactctt taaggaaaga aggatggaga aagagaaagg gagtagaggc
121 ggccacgacc tggtgaacac ctaggacgca ccattctcac aaagggagtt ttccacacgg
181 acacccccct cctcaccaca gccctgccag gacggggctg gctactggcc ttatctcaca
241 ggtaaaactg acgcacggag gaacaatata aattggggac tagaaaggtg aagagccaaa
301 gttagaactc aggaccaact tattctgatt ttgtttttcc aaactgcttc tcctcttggg
361 aagtgtaagg aagctgcagc accaggatca gtgaaacgca ccagacggcc gcgtcagagc
421 agctcaggtt ctgggagagg gtagcgcagg gtggccactg agaaccgggc aggtcacgca
481 tccccccctt ccctcccacc ccctgccaag ctctccctcc caggatcctc tctggcaccg
541 gttgggcatg gccaggtagc ctatgctgtg tctggacgtc ctcctgctgg tatagttatt
601 ttaaaatcag aaggacaggg aagggagcag tggttcacgc ctgtaatccc agcaatttgg
661 gaggccaagg tgggtagatc acctgagatt aggagttgga gaccagcctg gccaatatgg
721 tgaaaccccg tctctaccaa aaaaacaaaa attagctgag cctggtcatg catgcctgga
781 atcccaacaa ctcgggaggc tgaggcagga gaatcgcttg aacccaggag gcggagattg
841 cagtgagcca agattgtgcc actgcactcc agcttggttc ccaatagacc ccgcaggccc
901 tacaggttgt cttcccaact tgccccttgc tccataccac ccccctccac cccataatat
961 tatagaagga cacctagtca gacaaaatga tgcaacttaa ttttattagg acaaggctgg
1021 tgggcactgg agtggcaact tccagggcca ggagaggcac tggggagggg tcacagggat
1081 gccacccggg atctgttcag gaaacagcta tgaccgcggc cggccgttta aaccttatcg
1141 tcgtcatcct tgtaatccag gatatcattt gctgccagat cctcttctga gatgagtttc
1201 tgctcgagcg gccgcgtacg cgtcggtccg ctagctagtt aagcttgaga tctggcgcgc
1261 cggcgatcgc ggcggcagat ctcctcggta ccggatccag tcgacgaatt cccggccgcc
1321 ctatagtgag tcgtattaca aaattctgac ggttcactaa acgagctctg cttatataga
1381 cctcccaccg tacacgccta ccgcccattt gcgtcaacgg ggcggggtta ttacgacatt
1441 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
1501 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attggtgtac tgccaaaacc
1561 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
1621 gtaaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
1681 ggggcggact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
1741 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
1801 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
1861 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
1921 attaataact agtctgaaga ggagtttacg tccagccaag cttaggatct cgacctcgaa
1981 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
2041 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
2101 cggtaggcgc caaccgactc cgttctttgg tggccccttc gcgccacctt ctactcctcc
2161 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
2221 agtagcacgt ctcaccagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
2281 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
2341 gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg
2401 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
2461 ctcctcttcc tcatctccgg gcctttcgac ctgcatccat ctagatctcg agcagctgaa
2521 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
2581 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
2641 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
2701 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
2761 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
2821 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
2881 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
2941 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
3001 cttcctggag acctccgcgc cccacaacct ccccttctac gagcggctcg gcttcaccgt
3061 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
3121 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
3181 atcgatgata tcagatcccc gggatgcaga aattgatgat ctattaaaca ataaagatgt
3241 ccactaaaat ggaagttttt cctgtcatac tttgttaaga agggtgagaa cagagtacct
3301 acattttgaa tggaaggatt ggagctacgg gggtgggggt ggggtgggat tagataaatg
3361 cctgctcttt actgaaggct ctttactatt gctttatgat aatgtttcat agttggatat
3421 cataatttaa acaagcaaaa ccaaattaag ggccagctca ttcctcccac tcatgatcta
3481 tagatctata gatctctcgt gggatcattg tttttctctt gattcccact ttgtggttct
3541 aagtactgtg gtttccaaat gtgtcagttt catagcctga agaacgagat cagcagcctc
3601 tgttccacat acacttcatt ctcagtattg ttttgccaag ttctaattcc atcagaagct
3661 ggtcgagatc cggaaccctt aatataactt cgtataatgt atgctataga agttattagg
3721 tccactagtg ctgtcctgaa gtggacatag gggcccgggt tggaggaaga agactagctg
3781 agctctcgga cccctggaag atgccatgac agggggctgg aagagctagc acagactaga
3841 gaggtaaggg gggtagggga gctgcccaaa tgaaaggagt gagaggtgac ccgaatccac
3901 aggagaacgg ggtgtccagg caaagaaagc aagaggatgg agaggtggct aaagccaggg
3961 agacggggta ctttggggtt gtccagaaaa acggtgatga tgcaggccta caagaagggg
4021 aggcgggacg caagggagac atccgtcgga gaaggccatc ctaagaaacg agagatggca
4081 caggccccag aaggagaagg aaaagggaac ccagcgagtg aagacggcat ggggttgggt
4141 gagggaggag agatgcccgg agaggaccca gacacgggga ggatccgctc agaggacatc
4201 acgtggtgca gcgccgagaa ggaagtgctc tctagagtgt cgcatgaata acgggcagtt
4261 atcaaatgag cacggcgaca gcctgtgaaa gtcctctcca ctgactgtag cctccaattc
4321 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
4381 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
4441 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
4501 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
4561 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
4621 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
4681 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
4741 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
4801 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
4861 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
4921 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
4981 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
5041 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
5101 ctgctcccgg catccgctta cagacaagct gtgaccgtca acgggagctg catgtgtcag
5161 aggttttcac cgtcatcacc gaaacgcgcg acccgaaagg gcctcgtgat acgcctattt
5221 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
5281 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
5341 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
5401 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
5461 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
5521 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
5581 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
5641 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
5701 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
5761 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
5821 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
5881 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
5941 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
6001 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
6061 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggttc tcgcggtatc
6121 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
6181 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
6241 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
6301 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
6361 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
6421 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
6481 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
6541 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
6601 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
6661 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
6721 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
6781 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
6841 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
6901 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
6961 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
7021 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
7081 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
7141 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
7201 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
7261 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
7321 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
7381 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ccttcctctt
7441 ccagcccttc ctctttcggg cggcagcaag atcccttctg atctaaagtc caaccggcga
7501 gagtgat
//
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