LOCUS        pADH137      9185 bp    DNA     circular SYN 14-APR-2025
DEFINITION  NAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT 
            CRISPR system. Use with pADH118-series gRNA expression constructs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Nguyen N, Quail MMF, Hernday AD
  TITLE     An Efficient, Rapid, and Recyclable System for CRISPR-Mediated 
            Genome Editing in Candida albicans.
  JOURNAL   mSphere. 2017 Apr 26;2(2):e00149-17. doi: 
            10.1128/mSphereDirect.00149-17. eCollection 2017 Mar-Apr.
  PUBMED    28497115
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            //gt12365.cn
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/mSphereDirect.00149-17"; journalName: "mSphere"; date: 
            "2017-04-26- 26"; volume: "2"; issue: "2"; pages: "e00149-17"
COMMENT     Sequence Label: pADH137
FEATURES             Location/Qualifiers
     source          1..9185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     138..156
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1724..5827
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1724..5827
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5840..5860
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5864..5884
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5888..5908
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5909..5974
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      6039..6286
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6055..6073)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        6326..6669
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        complement(join(7177..7183,7184..7201,7202..7207))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7222..7243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7360..7377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7531..8119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7611..7630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8290..9081,9082..9150))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8913..8932
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9151..9185,1..70))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
       61 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
      121 tgacattaac ctataaaaat aggcgtatca cgaggccctt tgacggcacg gccacgcgtt
      181 taaaccgcca tgtctgttaa aaccaaaacc attactgtct tgcctggtga ccatgtcggt
      241 accgaaattg tcaatgaagc aatcaaagtc ttgaaagcaa ttgaagctgc cactccctac
      301 caaaaaatcc aatttgattt caaacaccac ttgattggtg gtgctgccat tgatgccact
      361 ggcgtcccgc ttccagatga tgctcttgaa agtgctaaat catccgatgc agttttgttg
      421 ggtgctgttg gtggacccaa atggggtact ggcactgttc gtcctgaaca aggattattg
      481 aaaatccgta aagaattgaa cctttatgcc aacattagac catgtaattt tgccagtgac
      541 tcgttattag aattgtctcc attaaaagct gaagtggtca aaggaaccaa tttaattatt
      601 gttcgtgaat tagttggtgg tatatatttt ggtgaacgtc aagaacaaga agaaagtgaa
      661 gacaaaaaaa cagcttggga tacagaaaag tatactgttg acgaagttac tcgtatcact
      721 cgtatggcag catttatggc tttgcagcac aatccaccat tgccaatatg gtctttggac
      781 aaagctaatg ttttggcatc gtcgagattg tggagaaaga ccgttgataa agtgatttct
      841 gaagaatttc cagctttgtc tgtgcagcat caattgattg attcggctgc aatgattttg
      901 atccaaaacc caaccaaatt gaacggtatc atcattacaa cagctaacaa ttacacaaaa
      961 aaaattctct tttcgatgta gcaactatct tcaactaaaa cattttctcc ttcggcccat
     1021 gattgtcctc cgggtcgaca gcaagccgtt acaattgaga tggaaagcga cctaccttca
     1081 ctcgataagg tgcttaattg tacttcatat aaatctggcc cggatctaaa caaatgagtt
     1141 ccattaagcc gtgggttctc aattagggtt tttgtttttg atttagaaaa aagagatcaa
     1201 gatttgttta caggtgatgc ctttttttag aacttatgcg ttgcaaaagt tgactaacga
     1261 tttctataag gtgatccaca ctaattatac aaacgtacaa acagacatac ttttcctgcg
     1321 ttcacctgat gttggccaga tttctctctt cattgcatag aacataacca cactagggca
     1381 acagaaaaaa aaaaaaaagt gcatcgggaa gttgtgttcc attcattata tgtctactac
     1441 tgcatatgag tagcccaccc accaccacca tagtaagttt ttgtgtatgc gcgccgtcag
     1501 gttatttcat ttctgaattt ttcaaccacc ttactccctt tattgttgat tgacaatttt
     1561 gctcacagta agatctttta gactccaatt aatataaaat aagtctgatt ttccaattcc
     1621 tgttttttct ttttttttct gtttctattt ctttcctttt ctcccttttt tttaattctt
     1681 cattcaatca tcaattgata attcaggaat attacaaccc gggatggata aaaagtatag
     1741 tattggttta gatattggta ctaactctgt gggttgggca gttatcactg acgaatataa
     1801 agttccatca aagaaattta aggtgttagg taacactgac agacactcaa taaaaaagaa
     1861 tcttatcggt gctcttttgt tcgactccgg tgaaactgcc gaggctacac gtttaaaaag
     1921 aacagcaaga agaagatata cccgtagaaa aaatagaata tgttatttac aagaaatctt
     1981 ttctaatgaa atggctaaag ttgatgattc ctttttccat agattggaag agtcattttt
     2041 ggttgaagaa gacaaaaagc atgagagaca tccaatcttt gggaatatag ttgatgaagt
     2101 ggcttaccat gaaaaatatc ctaccattta tcatttaaga aagaaattgg tagattcaac
     2161 tgataaagct gaccttagat taatctattt agcacttgcc catatgatta aatttagagg
     2221 tcattttttg attgaaggtg atttgaaccc agataattct gacgtggata aattatttat
     2281 tcaattagtc caaacctaca accaattatt tgaggaaaat ccaattaatg ctagtggtgt
     2341 cgatgccaaa gctatattat cagccagatt atcaaaatct agacgtttgg aaaatttgat
     2401 tgcccaattg ccaggagaaa aaaagaatgg attatttgga aacttgatcg cattatcatt
     2461 gggtttgaca ccaaatttta aatctaattt tgatttagct gaagatgcta aattacaatt
     2521 atcaaaagac acctatgacg acgatttgga caatttactt gctcaaattg gtgatcaata
     2581 tgcagatttg ttcttagctg ctaaaaactt atctgatgct attttgttgt ctgatatttt
     2641 gagagtgaac acagaaataa ccaaagctcc attatcagca tctatgatca aacgttatga
     2701 tgaacaccat caggatttga ctttattgaa agctttggtg agacaacaat tgccagagaa
     2761 gtataaagaa atctttttcg atcaatctaa aaacgggtat gcaggttata ttgatggggg
     2821 tgcctcccaa gaggaatttt acaaatttat aaaacctatt ttagaaaaga tggatgggac
     2881 tgaggaactt ttggtcaaat tgaacagaga agatttgtta cgtaaacaga gaacttttga
     2941 taatggtagt atacctcacc aaattcattt gggtgagttg catgcaattt taagaagaca
     3001 agaagatttt tatccatttt taaaagataa tagagaaaaa atcgagaaaa ttttaacctt
     3061 tagaattcca tactatgttg ggcctttggc tagaggtaat tcaagatttg cctggatgac
     3121 acgtaaatca gaagaaacta ttaccccttg gaattttgaa gaggttgttg ataaaggagc
     3181 atcagcacag agttttattg aaagaatgac caatttcgat aaaaacttac caaatgaaaa
     3241 agttttacca aaacattcct tgttatacga atattttact gtttacaatg aacttacaaa
     3301 ggttaaatat gttactgaag gtatgcgtaa gccagccttt ttatctggag aacagaaaaa
     3361 ggcaatagtt gatttattgt ttaaaacaaa tagaaaagtt actgttaaac aattaaaaga
     3421 agattacttt aagaaaattg aatgttttga ttcagttgaa atcagtggtg ttgaagacag
     3481 atttaatgct agtttaggaa cttaccatga tttacttaaa attatcaaag ataaagattt
     3541 cttggataac gaagaaaatg aagacatttt agaagacatt gttttaacct taactttatt
     3601 cgaagataga gagatgattg aagaacgttt gaagacttat gcacatttgt ttgacgataa
     3661 agtgatgaaa cagttgaaaa gaagacgtta tactggatgg ggtagattgt ctcgtaaatt
     3721 gatcaatgga attagagata aacaaagtgg taaaactatc ttggactttt tgaaatctga
     3781 cggatttgct aatagaaatt tcatgcaatt gatccacgac gatagtttga catttaaaga
     3841 agacatccaa aaggcccaag tgagtgggca aggtgattca ttacatgaac atattgcaaa
     3901 tttagccgga tctcctgcta ttaagaaagg gatattacaa actgttaaag ttgtggatga
     3961 attagtgaaa gtaatgggaa gacataaacc tgaaaacatt gtcattgaga tggcaagaga
     4021 aaatcaaact acacaaaaag gacagaaaaa tagtagagaa cgtatgaaaa gaatagaaga
     4081 gggtattaaa gaattgggta gtcaaatatt gaaagaacac ccagtggaaa atacccagtt
     4141 gcaaaatgaa aaattatatc tttactacct tcaaaatgga cgtgatatgt atgttgatca
     4201 ggaattagat ataaatagac tttcagatta tgatgtagat catatagttc cacaatcttt
     4261 cttgaaagat gattccatag acaataaagt attaactaga agtgataaaa atagaggtaa
     4321 aagtgataat gtcccaagtg aggaagtcgt caaaaagatg aaaaattact ggcgtcaact
     4381 tttgaatgct aaattaatta ctcaaagaaa atttgataat ttgactaaag cagaaagagg
     4441 tgggctttct gaattagata aagccgggtt cattaaaaga caattggtcg aaactagaca
     4501 aattactaaa catgttgccc aaattttaga ttcccgtatg aacactaagt atgacgaaaa
     4561 tgataagtta atacgtgagg ttaaagtcat tactttaaaa tcaaaacttg tctctgattt
     4621 cagaaaggat ttccaattct ataaagttag agaaattaat aattatcatc atgctcatga
     4681 tgcatatttg aatgctgtag ttggaactgc tttaatcaag aaatacccta aattagaatc
     4741 tgaatttgta tatggtgatt acaaagtcta tgatgttaga aagatgattg ctaaatcaga
     4801 acaagaaatt ggtaaagcta cagctaaata cttcttttac tctaacatta tgaatttctt
     4861 taaaacagaa attactttgg caaacggtga aattagaaaa agacctctta ttgaaacaaa
     4921 tggtgagact ggagagatag tttgggacaa agggcgtgat ttcgctactg ttagaaaagt
     4981 tttatcaatg ccacaagtta acattgtaaa gaaaacagag gttcaaactg gtggtttctc
     5041 aaaagaaagt attttgccta aaagaaatag tgataaattg attgccagaa aaaaggattg
     5101 ggatccaaag aaatatggtg gtttcgactc accaaccgta gcctattctg ttttggttgt
     5161 ggcaaaggtt gaaaagggta aaagtaaaaa gcttaaatca gtaaaagaac ttttgggtat
     5221 tacaataatg gaaagaagtt cctttgaaaa gaaccctatt gattttttgg aagctaaagg
     5281 ttataaggaa gtaaagaagg acttaataat caaattgcct aaatattctt tatttgaatt
     5341 agaaaatggg agaaaaagaa tgttggcttc tgctggagaa ttgcaaaagg gtaatgaatt
     5401 agcattgcct tccaaatatg ttaacttctt gtatttagct tcacactatg aaaagttgaa
     5461 agggtcacca gaagataacg agcaaaaaca attatttgtt gaacaacaca aacactactt
     5521 agatgagatt atagaacaaa ttagtgaatt cagtaaaaga gtgatattag ctgatgcaaa
     5581 tttagataaa gttttgtcag cctataacaa acatagagat aagccaatta gagaacaagc
     5641 agaaaacatt attcacttat ttacccttac caatttagga gcacctgctg ctttcaagta
     5701 ttttgataca acaattgatc gtaaaagata tacctcaaca aaagaagtct tagacgccac
     5761 cttaattcat caatcaatca ctggattgta tgagacaaga attgatttgt ctcaattggg
     5821 tggtgatgaa ggggctgatc ctaagaagaa aagaaaagtt gatccaaaga aaaagcgtaa
     5881 ggtggatcct aagaaaaaga gaaaggttga ctacaaagac catgacggtg attataaaga
     5941 tcatgacatc gactacaagg atgacgatga caagtgataa tgactgcaga gatccatcga
     6001 cctgccgcca agctaattcc gggcgaattt ctgtcgagtc atgtaattag ttatgtcacg
     6061 cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc
     6121 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
     6181 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
     6241 aaccttgctt tagaaggttt tgggacgctc gaaggcttta atttgcggcc agcttgcctc
     6301 gtccccgcct ggtcacccgg ccagcgacat ggaggcccag aataccctcc ttgacagtct
     6361 tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc ccatacatcc
     6421 ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa gcaaaaatta
     6481 cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg cgttgaattg
     6541 tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac tgaggttctt
     6601 ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca catccgaaca
     6661 taaacaaccg gatcctacaa ttatgtctac tactttggat gatactgctt atagatacag
     6721 aacttctgtt ccaggtgatg ctgaagctat tgaagctttg gatggttctt tcactaccga
     6781 tactgttttt agagttactg ctactggtga tggtttcact ttgagagaag ttccagttga
     6841 tccaccattg actaaggttt tcccagatga tgaatccgat gatgaatccg atgctggtga
     6901 agatggtgat ccagattcta gaactttcgt tgcttatggt gatgatggtg atttggctgg
     6961 tttcgttgtt gtttcttatt ctggttggaa cagaagattg actgttgaag atattgaagt
     7021 tgctccagaa catagaggtc atggtgttgg tagagctttg atgggtttgg ctactgaatt
     7081 cgccagagaa agaggtgctg gtcatttgtg gttggaagtt accaatgtta atcggtgttt
     7141 aaaccccagc gcctggcggg cgctcacaat tccacacaac atacgagccg gaagcataaa
     7201 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7261 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7321 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7381 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7441 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7501 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7561 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7621 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7681 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7741 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7801 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7861 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7921 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7981 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8041 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8101 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8161 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8221 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8281 gtctgacagt taccaatgct tgatcagtga ggcacctatc tcagcgatct gtctatttcg
     8341 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8401 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8461 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8521 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8581 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8641 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8701 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8761 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8821 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8881 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8941 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9001 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9061 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9121 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9181 ttatc
//