LOCUS        pDAC565      11297 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Mammalian expression of Csm-GFP complex (RNase mut) from separate 
            promoters (Used for RNA imaging).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Colognori D, Trinidad M, Doudna JA
  TITLE     Precise transcript targeting by CRISPR-Csm complexes.
  JOURNAL   Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9.
  PUBMED    36690762
REFERENCE   2  (bases 1 to 11297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            //gt12365.cn
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9."
COMMENT     Sequence Label: pDAC565
FEATURES             Location/Qualifiers
     source          1..11297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        49..252
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             292..309
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             316..339
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             346..366
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2650..2697
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        2728..2931
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2962..2971
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2971..2994
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3001..3021
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3409..3456
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        3487..3690
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3721..3730
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3730..3753
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3760..3780
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4465..4467,4468..4470,4471..5184)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4465..4467,4468..4470,4471..5181)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4510..4531)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4771..4790)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5118..5139
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5185..5232
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5263..5466
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      5497..5506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5506..5529
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5536..5556
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6463..6510
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        6541..6744
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      6775..6784
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6784..6807
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6814..6834
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7915..7962
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        7993..8196
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      8227..8236
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8236..8259
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             8266..8286
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9025..9072
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        9103..9351
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     9103..9123
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     9274..9293
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(9505..9523)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9591..9695
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9696..9764,9765..10556)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9914..9933)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10727..11297,1..18)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11216..11235
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tcttaagcgt gatgcggttt
       61 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      121 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      181 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      241 ataagcagag ctcgtttagt gaaccgtcag atctctagag ccgccaccat gcaccatcac
      301 catcaccatt ccggcgatta caaagacgat gacgataaga tggcgcctaa gaagaaacgc
      361 aaagtgcggg gcatgaagaa agaaaagatt gatctgtttt acggagccct gctgcacgac
      421 atcggaaagg tcatccagcg agcaaccgga gagcggaaga aacacgcact tgtgggcgcc
      481 gactggttcg acgagatcgc cgacaaccaa gtcatctcgg atcagatccg gtaccatatg
      541 gccaactacc agtctgataa gctcggcaac gatcacctgg cttacatcac ctacattgcc
      601 gacaacatcg cctccggtgt cgaccgccgg caatccaacg aagagtcaga cgaagatacc
      661 tccgcaaaga tctgggacac ctacacgaac caggccgaca tctttaacgt gttcggagcg
      721 cagaccgata agcggtactt caagcctacc gtgctgaatc tcaagtcgaa gcccaacttc
      781 gcgtccgcca cttacgaacc ctttagcaag ggcgattacg ctgccatcgc cacccggatt
      841 aagaacgaac tggccgagtt cgagttcaac caagtccaga ttgactccct gctcaacctt
      901 ttcgaggcta ctctctcctt cgtgccgtca agcaccaaca ctaaggaaat cgccgacatc
      961 tccctggccg accattcccg cttgactgct gccttcgctc tggcgatcta cgactacctg
     1021 gaggacaagg gtcggcacaa ctacaaagag gacctgttca ccaaagtgtc agcgttctat
     1081 gaagaagaag ccttcctgct ggcctccttc gacctgtcgg gaatccagga ctttatctac
     1141 aacattaaca tcgcaactaa cggcgcggcg aagcagctga aggcccggag cctctacctg
     1201 gactttatgt ccgagtacat cgccgatagc ctgctggaca agctgggact gaacagggct
     1261 aacatgcttt acgtcggcgg cggacacgcc tacttcgtcc tggccaacac cgaaaagact
     1321 gtggaaaccc tggtgcagtt tgagaaggat ttcaaccagt tcctgttggc aaacttccag
     1381 acccgcctct atgtggcctt tggctggggt tccttcgcgg ccaaggacat catgtccgag
     1441 ctgaatagcc ccgagtccta ccgccaagtg taccaaaagg cttcgcgcat gatctccaaa
     1501 aagaaaatct ccagatacga ctaccagaca ctgatgctcc tgaatcgcgg tggaaagtcc
     1561 tcagagagag agtgcgagat ttgccactcc gtggagaacc tggtgtccta ccacgaccag
     1621 aaagtctgtg acatttgccg gggactgtac cagttctcga aagaaattgc ccatgaccac
     1681 ttcatcatta ccgaaaatga ggggctgccg attggaccaa acgcgtgctt aaagggcgtg
     1741 gcattcgaaa agctgtccca agaagcgttc agccgggtct acgtgaagaa tgactataag
     1801 gccggtaccg tgaaggctac gcatgtgttc gtgggggatt accagtgcga cgagatctac
     1861 aactacgccg ccctgagcaa gaacgagaac ggcctaggca tcaagagact ggccgtggtc
     1921 cggctcgacg tggatgactt gggcgccgcc ttcatggccg gtttcagcca gcagggaaac
     1981 ggacaatact ccactctgtc aagatcggcc acattctccc ggagcatgtc gctgttcttc
     2041 aaagtgtaca ttaaccagtt cgcctccgac aagaagctga gcattatcta cgcgggcggc
     2101 gatgacgtgt tcgccattgg atcgtggcag gatatcatcg cgttcactgt ggaacttcgc
     2161 gaaaacttca tcaagtggac caacgggaag ctcaccctct ccgcggggat agggttgttc
     2221 gccgacaaga ctcctattag cctgatggct caccagaccg gggaactgga agaggccgcc
     2281 aagggcaacg aaaaggactc catctcgctg ttctcaagcg actacacttt caagtttgat
     2341 aggttcatca ctaacgtgta cgacgacaaa ctggaacaga ttagatactt cttcaaccat
     2401 caagacgaga ggggaaagaa cttcatctat aagcttattg agcttttgag gaaccacgac
     2461 cgcatgaata tggcacgcct cgcctattac ctcactcgcc tggaagaact gacccgggag
     2521 actgacaggg acaagttcaa gaccttcaag aacctgttct actcctggta caccaacaag
     2581 aacgataagg accggaagga agccgagctc gcgctcctgc tgtacatcta cgaaatcaga
     2641 aaggattaac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcgca
     2701 cacattagct agccgtcagc acacattgtg atgcggtttt ggcagtacat caatgggcgt
     2761 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2821 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2881 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     2941 aaccgtcaga tctctagagc cgccaccatg gattacaaag acgatgacga taagatggcg
     3001 cctaagaaga aacgcaaagt gcggggcatg accatcctga ccgacgagaa ctacgtggac
     3061 atcgccgaga aagccatcct gaagctggaa agaaacacca gaaatagaaa gaaccctgat
     3121 gccttcttcc tgaccacatc taagctgcgg aacctgctga gcctgacaag caccctgttc
     3181 gacgagagca aggtgaagga atacgacgcc ctgctggaca gaatcgctta tctgagagtg
     3241 cagttcgtgt accaggccgg cagagagatc gccgtgaaag atctgatcga gaaggcccag
     3301 atcctggaag ctctgaaaga gatcaaggac cgggaaaccc tgcagagatt ctgcagatac
     3361 atggaagccc tggtggccta cttcaagttc tacggcggca aggactgacg gcaataaaaa
     3421 gacagaataa aacgcacggt gttgggtcgt ttgttcagtt cttttgcctt actttcaatg
     3481 catgcggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     3541 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     3601 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     3661 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat ctctagagcc
     3721 gccaccatgg attacaaaga cgatgacgat aagatggcgc ctaagaagaa acgcaaagtg
     3781 cggggcatga ccttcgccaa gatcaaattc agcgcccaga tccggctgga aaccggcctg
     3841 cacatcggag gatctgatgc ctttgccgct atcggcgcca tcgccagccc tgtgatcaag
     3901 gaccccatca ccaacctgcc tatcatcccc ggctctagcc tgaagggcaa gatgagaaca
     3961 ctgctggcca aggtgtacaa cgaaaaggtg gccgagaagc ctagcgacga cagcgacatc
     4021 ctgagcagac tgttcggaaa tagcaaggat aagcggttca agatgggcag actgatcttc
     4081 cgggacgcct tcctgagcaa cgccgacgag ctggattctc tgggcgtgcg gagctacacc
     4141 gaggtgaagt tcgagaacac catcgataga atcaccgccg aggccaatcc tagacagatc
     4201 gagagagcca ttcggaactc aacattcgac ttcgagctga tctacgagat cactgatgag
     4261 aatgagaacc aggtcgagga agatttcaag gtgatcagag acggcctgaa gctgctggaa
     4321 ctggactacc tgggcggaag cggctccaga ggctacggca aagtggcttt tgagaacctg
     4381 aaagccacca cagtgttcgg caactacgac gtgaaaaccc tgaacgagct gctgaccgcc
     4441 gaagtgggcg gaggtgctgc cgcaatggtg agcaagggcg aggagctgtt caccggggtg
     4501 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     4561 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     4621 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     4681 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4741 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4801 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4861 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     4921 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     4981 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5041 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     5101 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     5161 ggcatggacg agctgtacaa gtgacggcaa taaaaagaca gaataaaacg cacggtgttg
     5221 ggtcgtttgt tctggattgc gagaatggac tagtagcaaa ctgtgatgcg gttttggcag
     5281 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     5341 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     5401 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     5461 agagctcgtt tagtgaaccg tcagatctct agagccgcca ccatggatta caaagacgat
     5521 gacgataaga tggcgcctaa gaagaaacgc aaagtgcggg gcatgactta caagctctac
     5581 attatgacct ttcaaaacgc ccacttcggt tccggcactc tggactcatc gaagctgacc
     5641 ttctccgcgg atagaatctt ctcggcactc gtgctcgagg ctctgaagat gggaaagctc
     5701 gacgccttct tggccgaggc caaccaggat aagttcactc tgaccgacgc gttcccattc
     5761 caattcggtc ctttcctgcc gaaaccgatt ggttacccca agcacgacca gatcgaccag
     5821 tctgtggacg tgaaggaagt ccgccgccaa gcgaagctgt ccaaaaagct ccagttcctg
     5881 gctctggaaa acgtcgacga ctacctgaac ggagagctgt ttgagaatga ggaacacgcc
     5941 gtgatcgaca cagtgaccaa gaaccagccc cataaagatg ataatctgta ccaagtggcc
     6001 accactcggt tctcgaacga cacctccctt tacgtgatcg ccaacgaatc cgatctgctg
     6061 aacgaactga tgagcagcct tcagtactcc gggctgggcg gcaaaaggtc ctcaggattc
     6121 ggcagatttg agctggacat ccagaacatt cccttggaac tgtccgaccg gctgacgaag
     6181 aaccacagcg acaaggtcat gtcacttacc accgccctcc cggtggacgc tgatctcgag
     6241 gaagcgatgg aagatggcca ttacctgttg accaagtcgt ccggattcgc attctcccac
     6301 gccaccaacg aaaactatcg gaagcaggac ctgtacaagt tcgcctccgg gagcaccttc
     6361 agcaagactt tcgagggaca gatcgtggac gtgcgccctc tcgatttccc tcacgccgtg
     6421 ctgaactacg ccaagccgct gttctttaag ctcgaagtct aacggcaata aaaagacaga
     6481 ataaaacgca cggtgttggg tcgtttgttc gcacattcaa aaacaggcaa ttggacaagc
     6541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     6601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     6661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     6721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatctctag agccgccacc
     6781 atggattaca aagacgatga cgataagatg gcgcctaaga agaaacgcaa agtgcggggc
     6841 atgaaaaatg actaccggac cttcaagctg agcctgctga ccctggctcc tatccacatc
     6901 ggcaacggcg agaagtacac cagcagagaa ttcatctacg agaacaagaa gttctacttc
     6961 cccgacatgg gcaagttcta caacaagatg gtggaaaaga gactggccga gaagttcgag
     7021 gccttcctga tccagaccag acccaacgcc agaaacaacc ggctgatttc ttttctgaac
     7081 gacaacagaa tcgccgaaag atcttttggc ggctacagca tcagtgaaac cggcctggaa
     7141 tctgataaga accctaacag cgccggagct atcaacgagg tgaacaaatt catccgggac
     7201 gccttcggaa atccttacat cccaggcagc agcctgaagg gcgccatccg caccatcctg
     7261 atgaacacca cacctaagtg gaacaacgag aacgccgtga acgacttcgg cagattccca
     7321 aaggaaaaca agaacctgat cccttgggga cctaagaaag gcaaggaata cgacgacctg
     7381 ttcaacgcca tcagagtgtc cgacagcaag cccttcgaca acaaaagcct gatcctcgtg
     7441 cagaagtggg actacagcgc caaaaccaac aaggccaagc ctctgcctct gtacagagag
     7501 tctatcagcc ctctgaccaa gatcgagttc gagataacaa caaccactga tgaggccggc
     7561 agactgatcg aggaactggg aaagcgggcc caggcctttt ataaggacta caaggccttt
     7621 ttcctgtctg aattccctga tgataagatc caggctaatc tgcaataccc catctacctg
     7681 ggcgccggca gcggcgcttg gacaaagacc ctgtttaagc aggccgacgg catcctgcag
     7741 cggagatact ccagaatgaa aaccaagatg gtcaagaagg gcgtgctgaa gctgacaaag
     7801 gcccctctga aaacagtgaa gatccccagc ggcaaccaca gcctggtgaa gaatcacgag
     7861 agcttctacg agatgggcaa agccaacttc atgatcaagg aaatcgacaa gtgacggcaa
     7921 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcatgtgtgc cggccggaga
     7981 ataaaagtct aagtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     8041 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     8101 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     8161 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatctct
     8221 agagccgcca ccatggatta caaagacgat gacgataaga tggcgcctaa gaagaaacgc
     8281 aaagtgcggg gcatgaaaaa gctcgtgttc acctttaagc ggatcgacca ccctgctcag
     8341 gacctggccg tgaaattcca cggcttcctg atggaacagc tggatagcga ctacgtggac
     8401 tacctgcacc agcagcagac caacccctac gccacaaagg tgatccaggg caaagagaac
     8461 acccagtggg tcgtgcatct gctgacagac gacatcgagg acaaggtgtt catgaccctg
     8521 ctgcagatca aggaagtgtc cctgaacgac ctgcctaagt tgtctgtgga aaaggtggaa
     8581 atccaggagc tgggcgctga taagctgctc gagatcttca acagcgagga aaaccagacc
     8641 tacttcagca tcatcttcga gacacctaca ggctttaaaa gccagggcag ctacgtgatc
     8701 ttccccagca tgcggctgat ctttcagagc ctgatgcaga agtacggcag actggtggaa
     8761 aaccagcctg agatcgagga agataccctg gactacctga gcgagcacag caccatcacc
     8821 aattacagac tggaaacaag ctacttcaga gtgcatagac agagaatccc cgccttccgg
     8881 ggcaagctga ccttcaaggt gcagggagcc cagacactga aggcctacgt gaagatgctg
     8941 ctgaccttcg gcgagtacag cggcctgggc atgaaaacca gcctgggaat gggcggcatc
     9001 aagctggaag aaagaaagga ctgacggcaa taaaaagaca gaataaaacg cacggtgttg
     9061 ggtcgtttgt tcgtgcgata gagggatcca gtgcaccggt tggagggcct atttcccatg
     9121 attccttcat atttgcatat acgatacaag gctgttagag agataattag aattaatttg
     9181 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     9241 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     9301 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgatataaac
     9361 ctaattacct cgagagggga cggaaacccg tcttcgatga agcgattcag aagacttgat
     9421 ataaacctaa ttacctcgag aggggacttt tttacatgtg tcagaggttt tcaccgtcat
     9481 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     9541 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg ctcggaaccc
     9601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9661 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9721 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9781 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9841 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9901 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9961 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    10021 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    10081 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    10141 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    10201 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    10261 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    10321 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    10381 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    10441 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    10501 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10561 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10621 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10681 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10741 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10801 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10861 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10921 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10981 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    11041 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    11101 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    11161 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    11221 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    11281 tgtgatgctc gtcaggg
//