LOCUS        pEF6-CSPG4-myc-his      12732 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of CSPG4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Yuan P, Zhang H, Cai C, Zhu S, Zhou Y, Yang X, He R, Li C, Guo S, Li
            S, Huang T, Perez-Cordon G, Feng H, Wei W
  TITLE     Chondroitin sulfate proteoglycan 4 functions as the cellular 
            receptor for Clostridium difficile toxin B.
  JOURNAL   Cell Res. 2015 Feb;25(2):157-68. doi: 10.1038/cr.2014.169. Epub 2014
            Dec 30.
  PUBMED    25547119
REFERENCE   2  (bases 1 to 12732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            //gt12365.cn
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cr.2014.169"; journalName: "Cell Res"; date: "2015-02"; 
            volume: "25"; issue: "2"; pages: "157-68"
COMMENT     Sequence Label: pEF6-CSPG4-myc-his
FEATURES             Location/Qualifiers
     source          1..12732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        351..1529
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          582..1520
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1477..1497
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1546..1565
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1546..1564
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8614..8643
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             8659..8676
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8699..8716)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8705..8929
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8975..9403
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9062..9081)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9272..9293
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9412..9432)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9417..9746
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9597..9732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9659..9678
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9794..9841
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9860..10258
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    10416..10537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10453..10472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10507..10526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10586..10602)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10586..10602)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10599..10621)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10610..10626
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10634..10640,10641..10658,10659..10664))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10679..10700
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10817..10834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10977..11565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11057..11076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11736..12527,12528..12596))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12359..12378
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12597..12701)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtatgcag
     1621 tccgggccgc ggcccccact tccagccccc ggcctggcct tggctttgac cctgactatg
     1681 ttggccagac ttgcatccgc ggcttccttc ttcggtgaga accacctgga ggtgcctgtg
     1741 gccacggctc tgaccgacat agacctgcag ctgcagttct ccacgtccca gcccgaagcc
     1801 ctccttctcc tggcagcagg cccagctgac cacctcctgc tgcagctcta ctctggacgc
     1861 ctgcaggtca gacttgttct gggccaggag gagctgaggc tgcagactcc agcagagacg
     1921 ctgctgagtg actccatccc ccacactgtg gtgctgactg tcgtagaggg ctgggccacg
     1981 ttgtcagtcg atgggtttct gaacgcctcc tcagcagtcc caggagcccc cctagaggtc
     2041 ccctatgggc tctttgttgg gggcactggg acccttggcc tgccctacct gaggggaacc
     2101 agccgacccc tgaggggttg cctccatgca gccaccctca atggccgcag cctcctccgg
     2161 cctctgaccc ccgatgtgca tgagggctgt gctgaagagt tttctgccag tgatgatgtg
     2221 gccctgggct tctctgggcc ccactctctg gctgccttcc ctgcctgggg cactcaggac
     2281 gaaggaaccc tagagtttac actcaccaca cagagccggc aggcaccctt ggccttccag
     2341 gcagggggcc ggcgtgggga cttcatctat gtggacatat ttgagggcca cctgcgggcc
     2401 gtggtggaga agggccaggg taccgtattg ctccacaaca gcgtgcctgt ggccgatggg
     2461 cagccccatg aggtcagtgt ccacatcaat gctcaccggc tggaaatctc cgtggaccag
     2521 taccctacgc atacttcgaa ccgaggagtc ctcagctacc tggagccacg gggcagtctc
     2581 cttctcgggg ggctggatgc agaggcctct cgtcacctcc aggaacaccg cctgggcctg
     2641 acaccagagg ccaccaatgc ctccctgctg ggctgcatgg aagacctcag tgtcaatggc
     2701 cagaggcggg ggctgcggga agctttgctg acgcgcaaca tggcagccgg ctgcaggctg
     2761 gaggaggagg agtatgagga cgatgcctat ggacattatg aagctttctc caccctggcc
     2821 cctgaggctt ggccggccat ggagctgcct gagccatgcg tgcctgagcc agggctgcct
     2881 cctgtctttg ccaatttcac ccagctgctg actatcagcc cactggtggt ggccgagggg
     2941 ggcacagcct ggcttgagtg gaggcatgtg cagcccacgc tggacctgat ggaggctgag
     3001 ctgcgcaaat cccaggtgct gttcagcgtg acccgagggg cacgccatgg cgagctcgag
     3061 ctggacatcc cgggagccca ggcacgaaaa atgttcaccc tcctggacgt ggtgaaccgc
     3121 aaggcccgct tcatccacga tggctctgag gacacctccg accagctggt gctggaggtg
     3181 tcggtgacgg ctcgggtgcc catgccctca tgccttcgga ggggccaaac atacctcctg
     3241 cccatccagg tcaaccctgt caatgaccca ccccacatca tcttcccaca tggcagcctc
     3301 atggtgatcc tggaacacac gcagaagccg ctggggcctg aggttttcca ggcctatgac
     3361 ccggactctg cctgtgaggg cctcaccttc caggtccttg gcacctcctc tggcctcccc
     3421 gtggagcgcc gagaccagcc tggggagccg gcgaccgagt tctcctgccg ggagttggag
     3481 gccggcagcc tagtctatgt ccaccgcggt ggtcctgcac aggacttgac gttccgggtc
     3541 agcgatggac tgcaggccag ccccccggcc acgctgaagg tggtggccat ccggccggcc
     3601 atacagatcc accgcagcac agggttgcga ctggcccaag gctctgccat gcccatcttg
     3661 cccgccaacc tgtcggtgga gaccaatgcc gtggggcagg atgtgagcgt gctgttccgc
     3721 gtcactgggg ccctgcagtt tggggagctg cagaagcagg gggcaggtgg ggtggagggt
     3781 gctgagtggt gggccacaca ggcgttccac cagcgggatg tggagcaggg ccgcgtgagg
     3841 tacctgagca ctgacccaca gcaccacgct tacgacaccg tggagaacct ggccctggag
     3901 gtgcaggtgg gccaggagat cctgagcaat ctgtccttcc cagtgaccat ccagagagcc
     3961 actgtgtgga tgctgcggct ggagccactg cacactcaga acacccagca ggagaccctc
     4021 accacagccc acctggaggc caccctggag gaggcaggcc caagcccccc aaccttccat
     4081 tatgaggtgg ttcaggctcc caggaaaggc aaccttcaac tacagggcac aaggctgtca
     4141 gatggccagg gcttcaccca ggatgacata caggctggcc gggtgaccta tggggccaca
     4201 gcacgtgcct cagaggcagt cgaggacacc ttccgtttcc gtgtcacagc tccaccatat
     4261 ttctccccac tctatacctt ccccatccac attggtggtg acccagatgc gcctgtcctc
     4321 accaatgtcc tcctcgtggt gcctgagggt ggtgagggtg tcctctctgc tgaccacctc
     4381 tttgtcaaga gtctcaacag tgccagctac ctctatgagg tcatggagcg gccccgccat
     4441 gggaggttgg cttggcgtgg gacacaggac aagaccacta tggtgacatc cttcaccaat
     4501 gaagacctgt tgcgtggccg gctggtctac cagcatgatg actccgagac cacagaagat
     4561 gatatcccat ttgttgctac ccgccagggc gagagcagtg gtgacatggc ctgggaggag
     4621 gtacggggtg tcttccgagt ggccatccag cccgtgaatg accacgcccc tgtgcagacc
     4681 atcagccgga tcttccatgt ggcccggggt gggcggcggc tgctgactac agacgacgtg
     4741 gccttcagcg atgctgactc gggctttgct gacgcccagc tggtgcttac ccgcaaggac
     4801 ctcctctttg gcagtatcgt ggccgtagat gagcccacgc ggcccatcta ccgcttcacc
     4861 caggaggacc tcaggaagag gcgagtactg ttcgtgcact caggggctga ccgtggctgg
     4921 atccagctgc aggtgtccga cgggcaacac caggccactg cgctgctgga ggtgcaggcc
     4981 tcggaaccct acctccgtgt ggccaacggc tccagccttg tggtccctca aggaggccag
     5041 ggcaccatcg acacggccgt gctccacctg gacaccaacc tcgacatccg cagtggggat
     5101 gaggtccact accacgtcac agctggccct cgctggggac agctagtccg ggctggtcag
     5161 ccagccacag ccttctccca gcaggacctg ctggatgggg ccgttctcta tagccacaat
     5221 ggcagcctca gcccccgcga caccatggcc ttctccgtgg aagcagggcc agtgcacacg
     5281 gatgccaccc tacaagtgac cattgcccta gagggcccac tggccccact gaagctggtc
     5341 cggcacaaga agatctacgt cttccaggga gaggcagctg agatcagaag ggaccagctg
     5401 gaggcagccc aggaggcagt gccacctgca gacatcgtat tctcagtgaa gagcccaccg
     5461 agtgccggct acctggtgat ggtgtcgcgt ggcgccttgg cagatgagcc acccagcctg
     5521 gaccccgtgc agagcttctc ccaggaggca gtggacacag gcagggtcct gtacctgcac
     5581 tcccgccctg aggcctggag cgatgccttc tcgctggatg tggcctcagg cctgggtgct
     5641 cccctcgagg gcgtccttgt ggagctggag gtgctgcccg ctgccatccc actagaggcg
     5701 caaaacttca gcgtccctga gggtggcagc ctcaccctgg cccctccact gctccgtgtc
     5761 tccgggccct acttccccac tctcctgggc ctcagcctgc aggtgctgga gccaccccag
     5821 catggagccc tgcagaagga ggacggacct caagccagga ccctcagcgc cttctcctgg
     5881 agaatggtgg aagagcagct gatccgctac gtgcatgacg ggagcgagac actgacagac
     5941 agttttgtcc tgatggctaa tgcctccgag atggatcgcc agagccatcc tgtggccttc
     6001 actgtcactg tcctgcctgt caatgaccaa ccccccatcc tcactacaaa cacaggcctg
     6061 cagatgtggg agggggccac tgcgcccatc cctgcggagg ctctgaggag cacggacggc
     6121 gactctgggt ctgaggatct ggtctacacc atcgagcagc ccagcaacgg gcgggtagtg
     6181 ctgcgggggg cgccgggcac tgaggtgcgc agcttcacgc aggcccagct ggacggcggg
     6241 ctcgtgctgt tctcacacag aggaaccctg gatggaggct tccgcttccg cctctctgac
     6301 ggcgagcaca cttcccccgg acacttcttc cgagtgacgg cccagaagca agtgctcctc
     6361 tcgctgaagg gcagccagac actgactgtc tgcccagggt ccgtccagcc actcagcagt
     6421 cagaccctca gggccagctc cagcgcaggc actgaccccc agctcctgct ctaccgtgtg
     6481 gtgcggggcc cccagctagg ccggctgttc cacgcccagc aggacagcac aggggaggcc
     6541 ctggtgaact tcactcaggc agaggtctac gctgggaata ttctgtatga gcatgagatg
     6601 ccccccgagc ccttttggga ggcccatgat accctagagc tccagctgtc ctcgccgcct
     6661 gcccgggacg tggccgccac ccttgctgtg gctgtgtctt ttgaggctgc ctgtccccag
     6721 cgccccagcc acctctggaa gaacaaaggt ctctgggtcc ccgagggcca gcgggccagg
     6781 atcaccgtgg ctgctctgga tgcctccaat ctcttggcca gcgttccatc accccagcgc
     6841 tcagagcatg atgtgctctt ccaggtcaca cagttcccca gccggggcca gctgttggtg
     6901 tccgaggagc ccctccatgc tgggcagccc cacttcctgc agtcccagct ggctgcaggg
     6961 cagctagtgt atgcccacgg cggtgggggc acccagcagg atggcttcca ctttcgtgcc
     7021 cacctccagg ggccagcagg ggcctccgtg gctggacccc aaacctcaga ggcctttgcc
     7081 atcacggtga gggatgtaaa tgagcggccc cctcagccac aggcctctgt cccgctccgg
     7141 ctcacccgag gctctcgtgc ccccatctcc cgggcccagc tgagtgtggt ggacccagac
     7201 tcagctcctg gggagattga gtacgaggtc cagcgggcac cccacaacgg cttcctcagc
     7261 ctggtgggtg gtggcctggg gcccgtgacc cgcttcacgc aagccgatgt ggattcaggg
     7321 cggctggcct tcgtggccaa cgggagcagc gtggcaggca tcttccagct gagcatgtct
     7381 gatggggcca gcccacccct gcccatgtcc ctggctgtgg acatcctacc atccgccatc
     7441 gaggtgcagc tgcgggcacc cctggaggtg ccccaagctt tggggcgctc ctcactgagc
     7501 cagcagcagc tccgggtggt ttcagatcgg gaggagccag aggcagcata ccgcctcatc
     7561 cagggacccc agtatgggca tctcctggtg ggcgggcggc ccacctcggc cttcagccaa
     7621 ttccagatag accagggcga ggtggtcttt gccttcacca acttctcctc ctctcatgac
     7681 cacttcagag tcctggcact ggctaggggt gtcaatgcat cagccgtagt gaacgtcact
     7741 gtgagggctc tgctgcatgt gtgggcaggt gggccatggc cccagggtgc caccctgcgc
     7801 ctggacccca ccgtcctaga tgctggcgag ctggccaacc gcacaggcag tgtgccgcgc
     7861 ttccgcctcc tggagggacc ccggcatggc cgcgtggtcc gcgtgccccg agccaggacg
     7921 gagcccgggg gcagccagct ggtggagcag ttcactcagc aggaccttga ggacgggagg
     7981 ctggggctgg aggtgggcag gccagagggg agggcccccg gccccgcagg tgacagtctc
     8041 actctggagc tgtgggcaca gggcgtcccg cctgctgtgg cctccctgga ctttgccact
     8101 gagccttaca atgctgcccg gccctacagc gtggccctgc tcagtgtccc cgaggccgcc
     8161 cggacggaag cagggaagcc agaaagcagc acccccacag gcgagccagg ccccatggca
     8221 tccagccctg agcccgctgt ggccaaggga ggcttcctga gcttccttga ggccaacatg
     8281 ttcagcgtca tcatccccat gtgcctggta cttctgctcc tggcgctcat cctgcccctg
     8341 ctcttctacc tccgaaaacg caacaagacg ggcaagcatg acgtccaggt cctgactgcc
     8401 aagccccgca acggcctggc tggtgacacc gagacctttc gcaaggtgga gccaggccag
     8461 gccatcccgc tcacagctgt gcctggccag gggccccctc caggaggcca gcctgaccca
     8521 gagctgctgc agttctgccg gacacccaac cctgccctta agaatggcca gtactgggtg
     8581 ggcggccgct cgagtctaga gggcccgcgg ttcgaacaaa aactcatctc agaagaggat
     8641 ctgaatatgc ataccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
     8701 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     8761 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     8821 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     8881 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     8941 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     9001 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     9061 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     9121 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     9181 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     9241 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     9301 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     9361 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     9421 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     9481 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     9541 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     9601 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     9661 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     9721 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     9781 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     9841 caaggtgagg aactaaacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga
     9901 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
     9961 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
    10021 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
    10081 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
    10141 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
    10201 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaagc
    10261 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
    10321 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
    10381 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
    10441 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10501 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
    10561 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    10621 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    10681 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    10741 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    10801 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    10861 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    10921 gaaagaacat gtgagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10981 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    11041 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    11101 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    11161 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    11221 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    11281 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    11341 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    11401 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    11461 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    11521 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    11581 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    11641 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    11701 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    11761 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    11821 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    11881 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11941 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    12001 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    12061 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    12121 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    12181 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    12241 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    12301 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    12361 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    12421 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    12481 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    12541 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    12601 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    12661 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    12721 ccacctgacg tc
//