LOCUS       pINDUCER21-puro_Gateway-3xFLAG       12131 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway Destination vector for doxycycline-inducible expression of 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12131)
  AUTHORS   Ma X, Mandausch FJ, Wu Y, Sahoo VK, Ma W, Leoni G, Hostiuc M, 
            Wintgens JP, Qiu J, Kannaiyan N, Rossner MJ, Wehr MC
  TITLE     Comprehensive split TEV based protein-protein interaction screening 
            reveals TAOK2 as a key modulator of Hippo signalling to limit 
            growth.
  JOURNAL   Cell Signal. 2024 Jan;113:110917. doi: 
            10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7.
  PUBMED    37813295
REFERENCE   2  (bases 1 to 12131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            //gt12365.cn
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Signal."; date: "2024-01"; volume: "113:110917. doi"; pages: " 
            10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7"
COMMENT     Sequence Label: pINDUCER21-puro_Gateway-3xFLAG
FEATURES             Location/Qualifiers
     source          1..12131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2825
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2850..2855,2856..2873,2874..2880)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2934..3593
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3000..3019)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3935..4240
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4119..4138
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4281..4405)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4458..4523
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4541..4567
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4787..5965
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5018..5956
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5913..5933
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    6035..6068
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(6075..6692,6693..6698,6699..6734,6735..6737,6738..6773,
                     6774..6776,6777..6812,6813..6821)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(6874..7447,7448..7450,7451..7459)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7041..7058)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7268..7287
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7479..8078
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7479..7498)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7975..7995
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8214..8233
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8521..9109
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8574..8594)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8992..9003)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9184..9417
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9536..9554)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9622..9726
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9727..9795,9796..10587)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9945..9964)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10758..11346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11247..11266
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11500..11517
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11757..11778
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11793..11798,11799..11816,11817..11823)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11831..11847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11836..11858
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11855..11871
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11855..11871
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11921..11940
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
     2761 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
     2821 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
     2881 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
     2941 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     3001 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     3061 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     3121 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     3181 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     3241 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     3301 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     3361 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     3421 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     3481 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     3541 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg
     3601 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     3661 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     3721 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     3781 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     3841 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     3901 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     3961 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     4021 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     4081 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     4141 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     4201 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     4261 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     4321 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     4381 gttcagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag
     4441 agggcccgcg gttcgaagac tacaaagacc atgacggtga ttataaagat catgacatcg
     4501 attacaagga tgacgatgac aagtaggtaa gtgattcgaa tacccatacg acgtcccaga
     4561 ctacgcttag taatgattaa ttaaactaga aattctaccg ggtaggggag gcgcttttcc
     4621 caaggcagtc tggagtaacc cacccaagat ctggcctccg cgccgggttt tggcgcctcc
     4681 cgcgggcgcc cccctcctca cggcgagccg cgtaagatct ggcctccgcg ccgggttttg
     4741 gcgcctcccg cgggcgcccc cctcctcacg gcgagcctag tcgtgaggct ccggtgcccg
     4801 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     4861 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     4921 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     4981 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     5041 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     5101 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     5161 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     5221 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     5281 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     5341 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     5401 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     5461 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     5521 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     5581 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     5641 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     5701 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     5761 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     5821 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     5881 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     5941 ttttcttcca tttcaggtgt cgtgaagcgg ccgccatgct agtaaattgt ccgctaaatt
     6001 ctggccgttt ttggcttttt tgttagacgg atccataact tcgtataatg tatgctatac
     6061 gaagttatgc caccatgtct agactggaca agagcaaagt cataaacgga gctctggaat
     6121 tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag
     6181 ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc
     6241 caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc
     6301 aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca catcgcgacg
     6361 gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc
     6421 agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg
     6481 ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag
     6541 aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc
     6601 tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg
     6661 gcctggagaa acagctaaag tgcgaaagcg gcgggccgac cgacgccctt gacgattttg
     6721 acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg
     6781 acgctcttga cgattttgac cttgacatgc tccccgggta actaagtaag gatccgcggc
     6841 cgcactagag gaattccgcc cctctccccc ccccccctct ccctcccccc cccctaacgt
     6901 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     6961 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     7021 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     7081 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     7141 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     7201 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     7261 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     7321 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     7381 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttg aaaaacacga
     7441 tgataagctt gccacaaccc acaaggagac gaccttccat gaccgagtac aagcccacgg
     7501 tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg
     7561 ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg
     7621 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     7681 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     7741 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     7801 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     7861 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     7921 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     7981 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     8041 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc atgcccgacg gcgaggatct
     8101 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     8161 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     8221 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     8281 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     8341 ctgatgtaca agtaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
     8401 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     8461 atgcaattgt tgttgtttag tccctcccaa ttcgatatca agcttatcgc gatagatcct
     8521 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     8581 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     8641 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     8701 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     8761 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     8821 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8881 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     8941 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     9001 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     9061 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt
     9121 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     9181 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     9241 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     9301 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     9361 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     9421 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     9481 gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc
     9541 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     9601 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     9661 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     9721 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     9781 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     9841 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     9901 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     9961 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    10021 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    10081 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    10141 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    10201 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    10261 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    10321 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    10381 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    10441 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    10501 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    10561 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    10621 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    10681 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    10741 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    10801 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    10861 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    10921 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    10981 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    11041 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    11101 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    11161 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    11221 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    11281 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    11341 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    11401 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    11461 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    11521 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    11581 agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
    11641 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
    11701 tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
    11761 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
    11821 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg
    11881 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    11941 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
    12001 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
    12061 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
    12121 aacttagtag t
//