| 質粒名稱: | pS-cr:GFP |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 196295 |
| 存儲實驗室: | Zhenghe Li |
|
載體骨架基本信息 |
|
| 載體名稱: | pCB301-2μ-HDV |
| 載體出品公司: | Plant Expression, CRISPR |
| 完整質粒大小: | 10153bp |
| 載體類型: | 哺乳細胞表達 |
| 篩選標記: | 新霉素（G418） |
| 載體抗性: | Kanamycin, 50 μg/mL |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 低拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: |
Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs、SpeI-DR-BsaI-BsaI-DR-GFP-SpeI
gRNA/shRNA sequenceggagaccgaggtccgaggtctcc
|
| 物種來源: | Synthetic |
| 啟動子: | duplicated cauliflower mosaic virus 35S promoter |
| 標簽/融合蛋白: | SpeI-DR-BsaI-DR-SpeI (N terminal on insert) |
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | SpeI (not destroyed) |
| 3’克隆位點: | SpeI (not destroyed) |
| 5’測序引物 | 35S promoter/forwarad primer: CTATCCTTCGCAAGACCCTTC |
| 3’測序引物 | Nos terminator/reverse primer: TCATCGCAAGACCGGCAA |
|
文獻引用方法 |
|
| 材料和方法部分: |
pS-cr:GFP was a gift from Zhenghe Li (Addgene plasmid # 196295 ; //n2t.net/addgene:196295 ; RRID:Addgene_196295)
|
| 參考文獻部分: | Engineered biocontainable RNA virus vectors for non-transgenic genome editing across crop species and genotypes. Liu Q, Zhao C, Sun K, Deng Y, Li Z. Mol Plant. 2023 Mar 6;16(3):616-631. doi: 10.1016/j.molp.2023.02.003. Epub 2023 Feb 7. 10.1016/j.molp.2023.02.003 PubMed 36751129 |
買家導航
pS-cr:GFP載體質粒基本信息
pS-cr:GFP質粒圖譜載體圖譜和pS-cr:GFP載體序列質粒序列多克隆位點信息
pS-cr:GFP質粒載體簡介
pS-cr:GFP質粒序列載體序列
LOCUS pS-cr:GFP 10153 bp DNA circular SYN 14-APR-2025
DEFINITION 35S promoter-driven expression of the positive-sense TSWV S RNA
segment encoding crRNA:GFP fusion in place of the NSs.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10153)
AUTHORS Liu Q, Zhao C, Sun K, Deng Y, Li Z
TITLE Engineered biocontainable RNA virus vectors for non-transgenic
genome editing across crop species and genotypes.
JOURNAL Mol Plant. 2023 Mar 6;16(3):616-631. doi:
10.1016/j.molp.2023.02.003. Epub 2023 Feb 7.
PUBMED 36751129
REFERENCE 2 (bases 1 to 10153)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.molp.2023.02.003"; journalName: "Mol Plant"; date:
"2023-03-6- 6"; volume: "16"; issue: "3"; pages: "616-631"
COMMENT Sequence Label: pS-cr:GFP
FEATURES Location/Qualifiers
source 1..10153
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 423..768
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 711..731
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
CDS complement(join(2241..2954,2955..2957,2958..2960))
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVPLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2286..2307)
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 2635..2654
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2894..2915
/label=EGFP-N
/note="EGFP, reverse primer"
terminator 3222..3474
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 3522..3546
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin 3674..5016
/label=2u ori
/note="yeast 2u plasmid origin of replication"
protein_bind complement(4637..4684)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
primer_bind 5057..5075
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(5113..5135)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 5235..5254
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 5275..5556
/gene="S. cerevisiae TRP1"
/label=TRP1 promoter
CDS 5557..6231
/codon_start=1
/gene="S. cerevisiae TRP1"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/label=TRP1
/note="yeast auxotrophic marker"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK"
rep_origin complement(6334..6789)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6471..6492)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6683..6702
/label=F1ori-R
/note="F1 origin, reverse primer"
CDS complement(6905..8053)
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
CDS complement(8352..9146)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 9355..9986
/label=oriV
/note="incP origin of replication"
misc_feature 10066..10090
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
ORIGIN
1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
61 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
121 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
181 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
241 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag
361 cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca
421 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
481 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
541 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
601 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
661 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
721 caagaccctt cctctatata aggaagttca tttcatttgg agaggagagc aattgtgtca
781 attttattca aaccttaaca ctcagtctta caaatcatca cattaaaacc ctaagaaacg
841 actgcggaat acagagttgt actttcgcac cttgaattac atacggtcaa agcatataac
901 aacttctacg atcatcatgt ctaaggttaa gctcactaag gaaaacattg ttgctttgtt
961 gacacaaggc aaagatcttg aatttgaaga agatcagaat ctggtagcat ttaacttcaa
1021 gactttttgt ctggaaaacc ttgaccagat caagaagatg agcattattt catgtctgac
1081 attcctgaag aatcgtcaga gtataatgaa ggttattaag caaagtgatt ttacttttgg
1141 caaaatcact ataaagaaaa cttcagacag gattggagcc actgacatga ccttcagaag
1201 gcttgatagc ttgatcaggg tcaggcttgt cgaggaaact gggaattctg agaatctcaa
1261 tactatcaaa tctaagattg cttctcaccc tctgattcaa gcctatggat tacctcttga
1321 tgatgcaaag tctgtgaggc ttgccataat gctgggaggt agcttacctc ttattgcttc
1381 agttgatagc tttgagatga tcagtgttgt cttggctata tatcaggatg caaaatacaa
1441 agacctcggg atcgatccaa agaagtatga caccagggaa gccttaggaa aagtttgcac
1501 tgtgctaaaa agcaaagcat ttgaaatgaa tgaagatcag gtgaagaaag ggaaagagta
1561 tgctgctata cttagctcca gcaatcctaa tgctaaagga agtattgcta tggaacatta
1621 cagtgaaact cttaacaagt tctatgaaat gttcggggtt aaaaaacaga caaaacttgc
1681 agaacttgct taaaagcagt tgtaagttaa attataaaaa agcctataaa tatataaagc
1741 tttctttatc tttattgctt gtgcttgctt agtgtgttaa atttaaaata agtgtgttta
1801 attaaagttt gctttctgtg tgttgtgctt aataataaac aaaataacaa aaacaacaaa
1861 aaacataaaa taaaaataag aaaaaataaa ataaaataaa ataaaataaa taaataaaaa
1921 taaaaataaa ataaaaacaa aaaacaaaaa acaaaaaaca aaacaaaacc caaatttggc
1981 caaaattgtc cctttcggga tctttttggg tttttgtttt ttaatttttt gttgttttta
2041 tttcattttt tgatttttgt ttgattttgt ttcatttttt gatttttgtt tcattttgat
2101 tttattttta tttttatttt tatttttatg gtttctatta aacaggagaa attcaaaaga
2161 aatgacaaaa cagagaaata attatgagta aagaaagaaa ataaacataa cataattagg
2221 aaaagctgga cacggcaaga ttacttgtac agctcgtcca tgccgagagt gatcccggcg
2281 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg
2341 gcggactggg tgctcaggta gtggttgtcg ggcagcggca cggggccgtc gccgatgggg
2401 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
2461 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
2521 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
2581 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
2641 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
2701 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg
2761 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
2821 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
2881 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
2941 tcctcgccct tgctcaccat actagtatct acacttagta gaaattggag accgaggtcc
3001 gaggtctcca tctacactta gtagaaatta ctagttatgg ttattggtac tgtgttctta
3061 ttacagtatt gtgattttct aagtgaggtt tgattaagaa taaaattatg acacaattgc
3121 tctgggtcgg catggcatct ccacctcctc gcggtccgac ctgggcatcc gaaggaggac
3181 gtcgtccact cggatggcta agggagagct cgaatttccc cgatcgttca aacatttggc
3241 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
3301 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
3361 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
3421 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcggaatt
3481 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg
3541 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
3601 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac aggagatctc agtaaagcga
3661 aaagtgccac ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag
3721 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
3781 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac
3841 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
3901 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat
3961 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt
4021 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag
4081 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt
4141 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat
4201 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt
4261 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata
4321 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat
4381 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga
4441 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata
4501 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta
4561 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg
4621 ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa
4681 cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca
4741 gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa
4801 gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga
4861 tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct
4921 tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc
4981 atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat
5041 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
5101 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
5161 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
5221 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata gatcaacgac
5281 attactatat atataatata ggaagcattt aatagaacag catcgtaata tatgtgtact
5341 ttgcagttat gacgccagat ggcagtagtg gaagatattc tttattgaaa aatagcttgt
5401 caccttacgt acaatcttga tccggagctt ttcttttttt gccgattaag aattaattcg
5461 gtcgaaaaaa gaaaaggaga gggccaagag ggagggcatt ggtgactatt gagcacgtga
5521 gtatacgtga ttaagcacac aaaggcagct tggagtatgt ctgttattaa tttcacaggt
5581 agttctggtc cattggtgaa agtttgcggc ttgcagagca cagaggccgc agaatgtgct
5641 ctagattccg atgctgactt gctgggtatt atatgtgtgc ccaatagaaa gagaacaatt
5701 gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag catataaaaa tagttcaggc
5761 actccgaaat acttggttgg cgtgtttcgt aatcaaccta aggaggatgt tttggctctg
5821 gtcaatgatt acggcattga tatcgtccaa ctgcatggag atgagtcgtg gcaagaatac
5881 caagagttcc tcggtttgcc agttattaaa agactcgtat ttccaaaaga ctgcaacata
5941 ctactcagtg cagcttcaca gaaacctcat tcgtttattc ccttgtttga ttcagaagca
6001 ggtgggacag gtgaactttt ggattggaac tcgatttctg actgggttgg aaggcaagag
6061 agccccgaaa gcttacattt tatgttagct ggtggactga cgccagaaaa tgttggtgat
6121 gcgcttagat taaatggcgt tattggtgtt gatgtaagcg gaggtgtgga gacaaatggt
6181 gtaaaagact ctaacaaaat agcaaatttc gtcaaaaatg ctaagaaata ggttattact
6241 gagtagtatt tatttaagta ttgtttgtgc acttgccgat ctatgcggtg tgaaataccg
6301 cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa
6361 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
6421 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
6481 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
6541 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
6601 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
6661 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
6721 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
6781 agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
6841 ctcttcgcta ttacgccaga tccttgctgg ctgaaccccc agccggaact gaccccacaa
6901 ggccctagcg tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc
6961 tgcctcgcaa ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg
7021 aatccgatcc gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg
7081 agctgaaata gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata
7141 tgaatttcgt gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct
7201 ggcaacggga cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg
7261 attccaggtg cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca
7321 ggcattcctc ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa
7381 gctcgtagaa cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca
7441 cctgctgcca caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct
7501 tcacgtcctt gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct
7561 tgttgcgcgt ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt
7621 ccggccacgg cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt
7681 gtttcagcaa cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg
7741 tttttcgctt cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac
7801 ctgccgcctc ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg
7861 cagggggagc cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tgggccatcg
7921 agccgacgga ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt
7981 cggcatcctc ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa
8041 acgtccgatt cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc
8101 cttattcctg atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa
8161 tgaagtcggt cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc
8221 cctgcacgaa taccagcgac cccttgccca aatacttgcc gtgggcctcg gcctgagagc
8281 caaaacactt gatgcggaag aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc
8341 acatctaggt actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca
8401 ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc
8461 ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca
8521 agatcaataa agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg
8581 ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg
8641 cgcggatctt taaatggggt gtcttcttcc cagttttcgc aatccacatc ggccagatcg
8701 ttattcagta agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa
8761 tccgatatgt cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt
8821 tcagggcttt gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg
8881 agcagattgc tccagccatc atgccgttca aggtgcagga cctttggaac aggcagcttt
8941 ccttccagcc atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac
9001 cggctgtccg tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta
9061 gcaggagaca ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat
9121 tccggtgata ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa
9181 agatacccca agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct
9241 aaaactttaa ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca
9301 tagtatcgac ggagccgatt ttgaaaccac aattatgggt gatgctgcaa ctcgagcggg
9361 ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg
9421 cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag
9481 gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg
9541 acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg
9601 tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg
9661 cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt
9721 cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat
9781 ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt
9841 cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac
9901 acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg
9961 cgagggcaac cagcccggtg agcgtctcta gtggactgat gggctgcctg tatcgagtgg
10021 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
10081 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta
10141 ctggggtggt ttt
//