| 質粒名稱: | pcDNA3-Lyn-ICUE3 |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 61623 |
| 存儲實驗室: | Jin Zhang |
|
載體骨架基本信息 |
|
| 載體名稱: | pcDNA3 |
| 載體出品公司: | Invitrogen |
| 空載體大小: | 5446bp |
| 完整質粒大小: | 9113bp |
| 載體類型: | 哺乳細胞表達 |
| 篩選標記: | 新霉素（G418） |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | Lyn-ICUE3 |
| 物種來源: | H. sapiens (human) |
| 插入大小: | 3708bp |
| 啟動子: | CMV |
| 標簽/融合蛋白: |
ECFP (N terminal on insert)
cpVenus[L194] (C terminal on insert)
N-terminal targeting sequence from Lyn kinase (N terminal on insert)
|
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | HindIII (not destroyed) |
| 3’克隆位點: | EcoRI (not destroyed) |
| 5’測序引物 | T7 forward |
| 3’測序引物 |
BGH reverse
(Common Sequencing Primers)
|
|
文獻引用方法 |
|
| 材料和方法部分: | pcDNA3-Lyn-ICUE3 was a gift from Jin Zhang (Addgene plasmid # 61623 ; //n2t.net/addgene:61623 ; RRID:Addgene_61623) |
| 參考文獻部分: | The role of membrane microdomains in shaping beta2-adrenergic receptor-mediated cAMP dynamics. DiPilato LM, Zhang J. Mol Biosyst. 2009 Aug;5(8):832-7. doi: 10.1039/b823243a. Epub 2009 May 26. 10.1039/b823243a PubMed 19603118 |
買家導航
pcDNA3-Lyn-ICUE3載體質粒基本信息
pcDNA3-Lyn-ICUE3質粒圖譜載體圖譜和pcDNA3-Lyn-ICUE3載體序列質粒序列多克隆位點信息
pcDNA3-Lyn-ICUE3質粒載體簡介
pcDNA3-Lyn-ICUE3質粒序列載體序列
LOCUS pcDNA3-Lyn-ICUE3 9123 bp DNA circular SYN 14-APR-2025
DEFINITION Plasma membrane-targeted FRET biosensor for monitoring cAMP.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9123)
AUTHORS DiPilato LM, Zhang J
TITLE The role of membrane microdomains in shaping beta2-adrenergic
receptor-mediated cAMP dynamics.
JOURNAL Mol Biosyst. 2009 Aug;5(8):832-7. doi: 10.1039/b823243a. Epub 2009
May 26.
PUBMED 19603118
REFERENCE 2 (bases 1 to 9123)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b823243a";
journalName: "Mol Biosyst"; date: "2009-08"; volume: "5"; issue:
"8"; pages: "832-7"
COMMENT Sequence Label: pcDNA3-Lyn-ICUE3
FEATURES Location/Qualifiers
source 1..9123
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 901..910
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS join(946..948,949..951,952..1662)
/codon_start=1
/product="monomeric super enhanced variant of CFP (Matsuda
et al., 2008)"
/label=mseCFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
CDS join(946..948,949..951,952..1662)
/codon_start=1
/product="cyan variant of GFP"
/label=CFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
CDS join(946..948,949..951,952..1662)
/codon_start=1
/product="enhanced CFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
/label=mECFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
CDS join(4027..4029,4030..4032,4033..4545)
/codon_start=1
/product="N-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VN173
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIE"
promoter complement(4675..4693)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4676..4693)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4713..4730)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4719..4943
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4989..5417
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5076..5095)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5286..5307
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5426..5446)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5431..5761
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5612..5747
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5674..5693
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5828..6622
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5882..5901)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6492..6511
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 6796..6917
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6833..6852)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6887..6906
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6966..6982)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6966..6982)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6979..7001)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6990..7006
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(7014..7020,7021..7038,7039..7044))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7059..7080
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7197..7214)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7368..7956)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7448..7467)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(8127..8918,8919..8987))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8750..8769
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8988..9092)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
901 gccaccatgg gctgcatcaa gagcaagcgc aaggacaagg atcccatggt gagcaagggc
961 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
1021 cacaggttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
1081 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
1141 acctggggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
1201 aagtccgcca tgcccgaagg ctacgtccag gagcgtacca tcttcttcaa ggacgacggc
1261 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
1321 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
1381 tacatcagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggcccac
1441 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
1501 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
1561 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
1621 accgccgccg ggatcactca cggcatggac gagctgtaca aggctggtac ccccgtggga
1681 actcatgaga tggaggagga gttggccgaa gctgtggccc tgctctccca gcgggggcct
1741 gacgccctgc tcactgtggc acttcgaaag cccccaggtc agcgcacgga tgaagagctg
1801 gacctcatct ttgaggagct gctgcacatc aaggctgtgg cccacctctc caactcggtg
1861 aagcgagaat tagcggctgt tctgctcttt gaaccacaca gcaaggcagg gaccgtgttg
1921 ttcagccagg gggacaaggg cacttcgtgg tacattatct ggaagggatc tgtcaacgtg
1981 gtgacccatg gcaaggggct ggtgaccacc ctgcatgagg gagatgattt tggacagctg
2041 gctctggtga atgatgcacc ccgggcagcc accatcatcc tgcgagaaga caactgtcat
2101 ttcctgcgtg tggacaagca ggacttcaac cgtatcatca aggatgtgga ggcaaagacc
2161 atgcggctgg aagaacatgg caaagtggtg ctggtgctgg agagagcctc tcagggcgcc
2221 ggcccttccc gacccccaac cccaggcagg aaccggtata cagtgatgtc tggcacccca
2281 gagaagatcc tagagcttct gttggaggcc atgggaccag attccagtgc tcatgaccaa
2341 acagagacat tcctcagcga cttcctcctg acccacaggg tcttcatgcc cagcgcccaa
2401 ctctgcgctg cccttctgca ccacttccat gtggagcctg cgggtggcag cgagcaggag
2461 cgcagcacct acgtctgcaa caagaggcag cagatcttgc ggctggtcag ccagtgggtg
2521 gccctgtatg gctccatgct ccacactgac cctgtggcca ccagcttcct ccagaaactc
2581 tcagacctgg tgggcaggga cacccgactc agcaacctgc tgagggagca gtggccagag
2641 aggcggcgat gccacaggtt ggagaatggc tgtgggaatg catctcctca gatgaaggcc
2701 cggaacttgc ctgtttggct ccccaaccag gacgagcccc ttcctggcag cagctgtgcc
2761 atccaagttg gggataaagt cccctatgac atctgccggc cagaccactc agtgttgacc
2821 ctgcagctgc ctgtgacagc ctccgtgaga gaggtgatgg cagcgttggc ccaggaggat
2881 ggctggacca aggggcaggt gctggtgaag gtcaattctg caggtgatgc cattggcctg
2941 cagccagatg cccgtggtgt ggccacatct ctggggctca atgagcgtct ctttgttgtc
3001 aacccacagg aagcgcatga gctgatccca caccctgacc agctggggcc cactgtgggc
3061 tctgctgagg ggctggacct ggtgagtgcc aaggacctgg caggccagct gacggaccac
3121 gactggagcc tcttcaacag tatccaccag gtggagctga tccactatgt gctgggcccc
3181 cagcatctgc gggatgtcac caccgccaac ctggagcgct tcatgcgccg cttcaatgag
3241 ctgcagtact gggtggccac cgagctgtgt ctctgccccg tgcccggccc ccgggcccag
3301 ctgctcagga agttcattaa gctggcggcc cacctcaagg agcagaagaa tctcaattcc
3361 ttctttgccg tcatgtttgg cctcagcaac tcggccatca gccgcctagc ccacacctgg
3421 gagcggctgc cccacaaagt ccggaagctg tactccgccc tcgagaggct gctggacccc
3481 tcatggaacc accgggtata ccgactggcc ctcgccaagc tctcccctcc tgtcatcccc
3541 ttcatgcccc ttcttctcaa agacatgacc ttcattcatg agggaaacca cacactagtg
3601 gagaatctca tcaactttga gaagatgaga atgatggcca gagccgcgcg gatgctgcac
3661 cactgccgaa gccacaaccc tgtgcctctc tcaccactca gaagccgagt ttcccacctc
3721 cacgaggaca gccaggtggc gaggatttcc acatgctcgg agcagtccct gagcacccgg
3781 agtccagcca gcacctgggc ttatgtccag cagctgaagg tcattgacaa ccagcgggaa
3841 ctctcccgcc tctcccgaga gctggagcca gagctcatgc ccgacaacca ctncctgagc
3901 taccagncca agntgagcaa agaccccaac gagaagcgcg atcacatggt cntgctggag
3961 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg aggtaccggt
4021 ggatctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
4081 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
4141 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
4201 cccaccctcg tgaccaccct gggctacggc cttcagtgct tcgcccgcta ccccgaccac
4261 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
4321 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
4381 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
4441 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag
4501 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
4561 ctcgccgacc actaccagca gaacaccccc nnnggngacg gccccgtgct gtaagaattc
4621 tgcagatatc catcacactg gcggccgctc gagcatgcat ctagagggcc ctattctata
4681 gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
4741 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
4801 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
4861 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
4921 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
4981 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
5041 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
5101 tctcgccacg ttcgccggct ttccccgtca agctctaaat cggggcatcc ctttagggtt
5161 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
5221 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
5281 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
5341 tgatttataa gggattttgg ggatttcggc ctattggtta aaaaatgagc tgatttaaca
5401 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
5461 ggctccccag gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
5521 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
5581 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
5641 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
5701 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
5761 aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc
5821 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
5881 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
5941 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
6001 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
6061 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
6121 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
6181 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
6241 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
6301 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
6361 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
6421 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
6481 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
6541 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
6601 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
6661 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
6721 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
6781 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
6841 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
6901 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
6961 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
7021 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
7081 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
7141 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
7201 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
7261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
7321 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
7381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
7441 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
7501 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
7561 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
7621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
7681 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
7741 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
7801 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
7861 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
7921 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
7981 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
8041 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
8101 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
8161 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
8221 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
8281 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
8341 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
8401 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
8461 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
8521 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
8581 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
8641 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
8701 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
8761 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
8821 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
8881 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
8941 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
9001 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
9061 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
9121 gtc
//
pcDNA3-Lyn-ICUE3載體圖譜質粒圖譜pdf版和pcDNA3-Lyn-ICUE3載體序列質粒序列等相關資料下載
pcDNA3-Lyn-ICUE3質粒載體應用舉例
- 上一篇：pV1081
- 下一篇：pCAG-VSFP Butterfly 1.2