| 質粒名稱: | pscALPSpuro-RsACE2 |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 159665 |
| 存儲實驗室: | Jeremy Luban |
|
載體骨架基本信息 |
|
| 載體名稱: | pscALPS puro |
| 載體出品公司: | Clontech |
| 空載體大小: | 10143bp |
| 完整質粒大小: | 10146bp |
| 載體類型: | 哺乳細胞表達, Lentiviral |
| 篩選標記: | Puromycin |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | rhinolophus sinicus ACE2 |
| 物種來源: | rhinolophus sinicus |
| 插入大小: | 2477bp |
| 基因突變: | T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T, N290Y relative to RpBphP2 |
| GenBank編號 | JN247409 |
| 啟動子: | SFFV |
| 標簽/融合蛋白: | Myc tag (C terminal on insert) |
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | XbaI (not destroyed) |
| 3’克隆位點: | EcoRI (not destroyed) |
| 5’測序引物 | CGCTTCTGTTCGCGCGCTTC |
| 3’測序引物 | GCGAACGCAGGCCTCAAGGT |
|
文獻引用方法 |
|
| 材料和方法部分: | pscALPSpuro-RsACE2 was a gift from Jeremy Luban (Addgene plasmid # 159665 ; //n2t.net/addgene:159665 ; RRID:Addgene_159665) |
| 參考文獻部分: | Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant. Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB, Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC, Kyratsous CA, Dudkina NV, Shen K, Luban J. Cell. 2020 Sep 15. pii: S0092-8674(20)31229-0. doi: 10.1016/j.cell.2020.09.032. 10.1016/j.cell.2020.09.032 PubMed 32991842 |
買家導航
pscALPSpuro-RsACE2載體質粒基本信息
pscALPSpuro-RsACE2質粒圖譜載體圖譜和pscALPSpuro-RsACE2載體序列質粒序列多克隆位點信息
pscALPSpuro-RsACE2質粒載體簡介
pscALPSpuro-RsACE2質粒序列載體序列
LOCUS pscALPSpuro-RsACE2 10146 bp DNA circular SYN 14-APR-2025
DEFINITION Expresses Rhinolophus sinicus ACE2 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10146)
AUTHORS Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang
Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB,
Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC,
Kyratsous CA, Dudkina NV, Shen K, Luban J
TITLE Structural and Functional Analysis of the D614G SARS-CoV-2 Spike
Protein Variant.
JOURNAL Cell. 2020 Sep 15. pii: S0092-8674(20)31229-0. doi:
10.1016/j.cell.2020.09.032.
PUBMED 32991842
REFERENCE 2 (bases 1 to 10146)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020
Sep 15. pii: S0092-8674(20)31229-0. doi:
10.1016/j.cell.2020.09.032."
COMMENT Sequence Label: pscALPSpuro-RsACE2
FEATURES Location/Qualifiers
source 1..10146
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(240..260)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 245..574
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 425..560
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 487..506
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
enhancer 586..965
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 967..1165
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1120..1140
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 1183..1363
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1410..1535
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 2032..2265
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2449..2493
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2791..2908
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 3045..3452
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
CDS 5895..5924
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 6428..7027
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(6428..6447)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6924..6944
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 7037..7625
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7090..7110)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7508..7519)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 8143..8323
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(8394..8982)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8474..8493)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(9153..9944,9945..10013))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9776..9795
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10014..10118)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgctgtg
241 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
301 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
361 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
421 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
481 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
541 aggaggcttt tttggaggcc taggcttttg caaaaagctc gcgttgacat tgattattga
601 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
661 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
721 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
781 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
841 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
901 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
961 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
1021 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
1081 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
1141 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
1201 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
1261 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
1321 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
1381 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
1441 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
1501 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
1561 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
1621 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
1681 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
1741 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
1801 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
1861 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
1921 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
1981 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
2041 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
2101 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
2161 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
2221 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
2281 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
2341 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaat taacaattac
2401 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
2461 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
2521 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
2581 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
2641 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
2701 gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt
2761 ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
2821 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
2881 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
2941 ttggttaatt aactgcagcc ccgataaaat aaaagatttt atttagtctc cagaaaaagg
3001 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg ccattttgca
3061 aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg ggtacatgaa
3121 aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc ccggcccggg
3181 gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa cagatggtcc
3241 ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc caggctcccc
3301 caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg cttctcgctt
3361 ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc tcactcggcg
3421 cgccagtcct ccgacagact gagtcgcccg ggggtctaga agccgccacc atgagctcta
3481 gctcatggct gctcctgagc ttggttgccg tcacgacggc acagtttacg acagaagatc
3541 tcgcaaaaat ttttttggat gagttcaatt ccgaagcaga gaatttgtca taccaatcca
3601 gccttgccag ctgggactat aacacaaata tcaatgatga aaacgttcaa aaaatggatg
3661 aagcgggcgc aaaatggtcc gcgttttatg aagaacaatc caaactcgct aaaaactatc
3721 cactggaaca aatacaaaat gtgactgtca aactgcagct gcaaatcttg caacaaagtg
3781 gctcaccagt actttctgag gacaaaagca agcgactcaa ttcaattctg aatgcgatgt
3841 ccaccatcta ctcaacggga aaagtttgca aaccgaacaa gccacatgag tgtttgctcc
3901 ttgagcccgg gctggataat ataatgggta ccagcaaaga ctatagcgaa aggctgtggg
3961 cctgggaagg gtggagggca gaggtaggaa agcagctgcg gccactgtac gaagagtatg
4021 tcgttctcaa aaacgaaatg gctcgcgggt atcactatga agactacggg gactactgga
4081 ggcgggatta tgaaaccgag gagtccccag ggccgggtta ctcaagggac cagctcatga
4141 aggacgtcga gcgcatcttc acagagatca agccgctcta tgagcatctt cacgcctacg
4201 ttcgagcgaa gcttatggac acatatccgt tccatatatc tccaacgggc tgcctgccag
4261 cacatcttct gggcgatatg tggggtcgat tctggacgaa cctttaccct ctcacagtac
4321 cgttcgggca aaagccgaat atagacgtta cggatgagat gctcaagcag ggttgggatg
4381 ctgatcggat attcaaggaa gcagaaaagt tttttgtatc tgttgggctt cccaatatga
4441 ccgaaggatt ctggaataat tccatgctta ccgagcccgg agacggtcgc aaagtggttt
4501 gccaccctac ggcctgggat cttggtaaag gagattttcg aataaaaatg tgcactaaag
4561 taaccatgga agacttcttg acagcgcatc atgagatggg acacatacag tacgacatgg
4621 cctatgctag tcagccctat ctcttgcgga acggggccaa cgaaggtttc catgaagcgg
4681 tcggcgaagt gatgtccctt tcagtagcga ctcccaaaca cttgaagact atgggacttc
4741 tcagcccgga cttcagggaa gataatgaga ctgaaataaa ctttcttctt aagcaggccc
4801 tcaatattgt tggtacactt ccgtttactt acatgctgga aaaatggagg tggatggtct
4861 tcaagggcga aattccgaag gaggaatgga tgaagaaatg gtgggagatg aagcggaaga
4921 ttgtaggggt agtagagcct gttccgcatg atgaaacata ttgtgacccg gcgtcccttt
4981 ttcatgtcgc caatgattac agcttcatcc gctattatac gagaactatt ttcgagttcc
5041 aatttcacga agccttgtgc aggattgctc aacatgacgg accccttcat aaatgcgata
5101 tatccaacag tacggatgca gggaaaaagt tgcatcaaat gctctccgtg ggaaaaagcc
5161 aagcctggac gaagacgctt gaagatatcg tagattcacg aaacatggac gttggtccac
5221 ttctccgata ctttgaacca ctttacacgt ggctgcaaga gcagaatcgc aagtcctatg
5281 ttggctggaa cacagattgg agtccgtaca gtgatcagtc catcaaggtg cggatctcac
5341 tcaagtctgc gttgggggag aacgcatatg agtggaacga caacgaaatg tatcttttcc
5401 gctcaagtgt tgcgtacgcg atgagggaat atttcctcaa agaaaaacat caaactattc
5461 tctttggcgc tgaaaatgtc tgggtctcca atttgaaacc acgaattagc tttaactttc
5521 atgtaaccag tcccggaaat ctctccgaca tcataccgag gcccgaggtc gaaggtgcga
5581 tacggatgag caggagtagg attaatgacg catttcgcct ggatgacaac tccctcgagt
5641 tccttggcat acaaccgacg ctcgggccac cctaccaacc tccggtcacg atctggctga
5701 tcgtgtttgg cgtagttatg gcagttgttg tagtgggcat tgttgtactc atcatcacag
5761 gcatacgaga cagaagaaaa acagaccagg caaggtcaga ggagaaccct tatagttccg
5821 ttgaccttag taaaggtgag aataatcccg gttttcagaa cggcgacgac gtccaaacgt
5881 catttggagc agctgagcag aagctgatca gcgaggagga cctgtaggaa ttctcgacct
5941 tgactggcgg cgcgaccttg aggcctgcgt tcgcctcagt tgccccctct gtgcaatggg
6001 gagacgcgcc tcatcgcttg acaacggccg aagagccgcc gcgcttccgt ctcccgcgtg
6061 cgcgcgccat gctgcccacc cccgttccgc actgaccctc ccccgtgccc cgcgtcccgt
6121 actgccgccc cgccccgagt cccatgccgc agccaccgcg acggagcccg caggcgggaa
6181 cctgcctccg cgcgttagcg cgcacgcgcg cctcatgtgt cgtccccatc agcgccggct
6241 tccgtctata ggccagatgc actgtcactc tggcgaagtc gcagacccga ttggccggga
6301 cggaggcgcg agaccgggtt gcgggcgggg ccgaacgtgg tataaaaggg gcgggaggcc
6361 aggctcgtgc cgttttgcag acgccaccgc cgaggaaaac cgtgtactat tagccacgcg
6421 tgccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg
6481 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
6541 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
6601 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
6661 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
6721 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
6781 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
6841 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
6901 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
6961 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
7021 tgcctgagcg atcgctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
7081 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
7141 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
7201 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
7261 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
7321 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
7381 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
7441 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
7501 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
7561 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
7621 cccgcttaat cgcgtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca
7681 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
7741 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
7801 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc
7861 cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca
7921 gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
7981 caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc
8041 ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta
8101 gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac
8161 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
8221 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
8281 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttcatgtga
8341 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
8401 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
8461 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
8521 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
8581 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
8641 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
8701 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
8761 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
8821 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
8881 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
8941 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
9001 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
9061 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
9121 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
9181 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
9241 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
9301 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
9361 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
9421 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
9481 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
9541 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
9601 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
9661 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
9721 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
9781 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
9841 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
9901 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
9961 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
10021 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
10081 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
10141 cctgac
//
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