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BL21 Star(DE3)pLySs

價格：800元

聯系方式：I47-825O-882O

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BL21(DE3)Star pLySs感受態細胞基本信息

出品公司:	Invitrogen
感受態細胞名稱:	BL21 Star(DE3)pLySs, BL21(DE3)Star pLySs
菌株類型:	E.coli
產品目錄號:	C6020-03
培養基:	LB培養基
生長條件:	37 ℃, 有氧
基因型:
抗性:	氯霉素
應用:	蛋白表達，IPTG誘導表達
菌株特點:	來源于BL21(DE3)菌株。含有rne131基因突變體，rne131突變基因能夠增強菌株細胞內mRNA的穩定性，從而有效提高蛋白表達能力。主要適用于T7啟動子載體的高水平蛋白表達。含有pLSs質粒，pLySs質粒能夠產生T7溶菌酶，可以有效降低目的基因的基礎表達水平。pLySs質粒使得該菌株具有氯霉素抗性。pLySs質粒的起始復制位點是p15 Origin, 這使得該質粒能夠和pUC-及pBR322-衍生的質粒互相共存。目的基因在菌株中的基礎表達水平，一般是BL21 Star(DE3)要高于BL21 Star(DE3)pLySs，但是誘導后的總共的蛋白表達水平仍然是BL21 Star(DE3)要高于BL21 Star(DE3)pLySs。總得來說，目的基因的表達水平在BL21 Star(DE3)要高于在BL21 Star(DE3)pLySs中。主要用來表達有毒蛋白或者對蛋白生長具有抑制作用的基因蛋白。建議選擇該菌株進行蛋白表達的條件如下：1. 你在使用高拷貝的T7啟動子載體進行蛋白表達。2.使用BL21 Star(DE3)進行蛋白表達時，你觀察到明顯的對細菌生長的抑制作用。3.你在表達一個已知的毒性蛋白。

JM109(DE3) Chemically Competent Cell	C58C1 Chemically Competent Cell
EGY48 Chemically Competent Cell	LBA4404 Chemically Competent Cell
BL21 Star(DE3)pLysS Chemically Competent	XL10-Gold Chemically Competent Cell
Mach1-T1 Chemically Competent Cell	K599 Chemically Competent Cell
Ar 1193 Chemically Competent Cell	MSU440 Chemically Competent Cell
Ar.Qual Chemically Competent Cell	Ar.A4 Chemically Competent Cell
AGL1(pSoup) Chemically Competent Cell	EHA105(pSoup) Chemically Competent Cell
GV3101 (pJIC SA_Rep) Chemically Competen	GV3101(pSoup-p19) Chemically Competent C
GV3101(pSoup) Chemically Competent Cell	EHA101 Chemically Competent Cell
AGL1 Chemically Competent Cell	YM4271 Chemically Competent Cell
R5421 Chemically Competent Cell	Y190 Chemically Competent Cell
BY4741 Chemically Competent Cell	INVSc1 Chemically Competent Cell
NMY51 Chemically Competent Cell	AH109 Chemically Competent Cell
Y187 Chemically Competent Cell	Y2HGold Chemically Competent Cell
Y1HGold Chemically Competent Cell	SHuffle T7 E. coli Chemically Competent
ArcticExpress (DE3) pRARE2 Chemically Co	ArcticExpress (DE3) Chemically Competent
HT115(DE3) Chemically Competent Cell	M15(pREP4) Chemically Competent Cell
ER2566 Chemically Competent Cell	C43(DE3) pLysS Chemically Competent Cell
OverExpress C43(DE3) Chemically Competen	TB1 Chemically Competent Cell
OrigamiB(DE3)pLysS Chemically Competent	Rosetta-gami B(DE3) Chemically Competent
Rosetta 2(DE3) Chemically Competent Cell	Rosetta(DE3) Chemically Competent Cell
BL21-CodonPlus (DE3)-RIPL Chemically Com	BL21(AI) Chemically Competent Cell
BW25113 Chemically Competent Cell	JM108 Chemically Competent Cell
MG1655 Chemically Competent Cell	JM330 Chemically Competent Cell
DH5α λpir Chemically Competent Cell	S17-1 λpir Chemically Competent Cell
MC1061F- Chemically Competent Cell	GT115 Chemically Competent Cell
EPI300 Chemically Competent Cell	EPI400 Chemically Competent Cell
TOP10F` Chemically Competent Cell	Turbo Chemically Competent Cell
Stable Chemically Competent Cell	BJ5183-AD-1 Chemically Competent Cell
HB101 Chemically Competent Cell	TG1 Chemically Competent Cell
DB3.1 Chemically Competent Cell	XL2-Blue Chemically Competent Cell
XL1-Blue MRF` Kan Chemically Competent C	JM110 Chemically Competent Cell
TOP10	DH5α
Origami 2(DE3)	DH10Bac
Stbl4感受態細胞	Stbl3 Ultracompetent Cells
Origami 2	Origami 2(DE3)pLysS
Origami 2(DE3)pLacI	Origami B
OrigamiB(DE3)pLysS	OrigamiB(DE3)pLacI
GV3101	BL21(DE3)
Origami(DE3)	OrigamiB(DE3)
Origami	Origami(DE3)pLysS
Origami(DE3)pLacI	BL21 Star(DE3)
BL21 Star(DE3)pLySs	BL21-AI
Sure	M15[pREP4]
One Shot ccdB Survival 2 T1R Competent C	BL21-Gold(DE3)
Rosetta(DE3)	Rosetta(DE3)pLySs
Rosetta-gami(DE3)	BL21(DE3)pLySs
RosettaBlue(DE3)	BL21
Rosetta-gami 2(DE3)	Rosetta-gami(DE3)pLysS
Rosetta-gami 2(DE3)pLysS	Rosetta-gami B(DE3)

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BL21 Star(DE3)pLySs

買家導航

BL21(DE3)Star pLySs感受態細胞基本信息

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