| 質粒名稱: | pBS302 |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 11925 |
| 存儲實驗室: | Brian Sauer |
|
載體骨架基本信息 |
|
| 載體名稱: | n/a |
| 載體出品公司: | Clontech |
| 空載體大小: | 3806bp |
| 完整質粒大小: | 3807bp |
| 載體類型: | 哺乳細胞表達, Cre/Lox |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | STOP |
| 克隆方法: | 限制性內切酶 |
| 5’測序引物 | na |
|
文獻引用方法 |
|
| 材料和方法部分: | pBS302 was a gift from Brian Sauer (Addgene plasmid # 11925 ; //n2t.net/addgene:11925 ; RRID:Addgene_11925) |
| 參考文獻部分: | Manipulation of transgenes by site-specific recombination: use of Cre recombinase. Sauer B. Methods Enzymol. 1993 . 225():890-900. PubMed 8231893 |
買家導航
pBS302載體質粒基本信息
pBS302質粒圖譜載體圖譜和pBS302載體序列質粒序列多克隆位點信息
pBS302質粒載體簡介
pBS302質粒序列載體序列
LOCUS pBS302 3807 bp DNA circular SYN 14-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3807)
AUTHORS Sauer B
TITLE Manipulation of transgenes by site-specific recombination: use of
Cre recombinase.
JOURNAL Methods Enzymol. 1993 . 225():890-900.
PUBMED 8231893
REFERENCE 2 (bases 1 to 3807)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Methods
Enzymol. 1993 . 225():890-900."
COMMENT Sequence Label: pBS302
FEATURES Location/Qualifiers
source 1..3807
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 50..68
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
protein_bind 118..151
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
polyA_signal 193..327
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(217..236)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 271..290
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
CDS complement(752..772)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
intron 902..967
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
protein_bind 1501..1534
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
primer_bind complement(1619..1641)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 1727..1867
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 1782..1801
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(1882..1899)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2053..2641)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2133..2152)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(2812..3603,3604..3672))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3435..3454
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3673..3777)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gcggccgcac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
61 cacgaggccc tttcgtcttc aagaattcca tcaagcttag gatccggaac ccttaatata
121 acttcgtata atgtatgcta tacgaagtta ttaggtccct cgacctgcag cccaagctta
181 cttaccatgt cagatccaga catgataaga tacattgatg agtttggaca aaccacaact
241 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
301 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
361 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
421 gctgattatg atctctagtc aaggcactat acatcaaata ttccttatta acccctttac
481 aaattaaaaa gctaaaggta cacaattttt gagcatagtt attaatagca gacactctat
541 gcctgtgtgg agtaagaaaa aacagtatgt tatgattata actgttatgc ctacttataa
601 aggttacaga atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg
661 tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta
721 gcaattctga aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag
781 aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca
841 ggttttcctc attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa
901 tctaaaatac acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta
961 tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga
1021 agtaaggttc cttcacaaag atccctcgag aaaaaaaata taaaagagat ggaggaacgg
1081 gaaaaagtta gttgtggtga taggtggcaa gtggtattcc gtaagaacaa caagaaaagc
1141 atttcatatt atggctgaac tgagcgaaca agtgcaaaat ttaagcatca acgacaacaa
1201 cgagaatggt tatgttcctc ctcacttaag aggaaaacca agaagtgcca gaaataacat
1261 gagcaactac aataacaaca acggcggcta caacggtggc cgtggcggtg gcagcttctt
1321 tagcaacaac cgtcgtggtg gttacggcaa cggtggtttc ttcggtggaa acaacggtgg
1381 cagcagatct aacggccgtt ctggtggtag atggatcgat ggcaaacatg tcccagctcc
1441 aagaaacgaa aaggccgaga tcgccatatt tggtgtcccc gaggatccgg aacccttaat
1501 ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgaagag gttcactagt
1561 actggccatt gcggccgctt cgaggctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
1621 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
1681 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
1741 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
1801 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
1861 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
1921 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
1981 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
2041 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
2101 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
2161 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
2221 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
2281 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
2341 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
2401 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
2461 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
2521 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
2581 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
2641 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
2701 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
2761 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
2821 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
2881 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
2941 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
3001 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
3061 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
3121 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
3181 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
3241 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
3301 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
3361 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
3421 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
3481 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
3541 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
3601 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
3661 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
3721 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
3781 atttccccga aaagtgccac ctgacgt
//
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