LOCUS       pCE35        9205 bp    DNA     circular SYN 14-APR-2025
DEFINITION  C. auris LEUpOUT NAT marker CAS9 expression construct. Use with 
            pCE27 gRNA expression construct..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Ennis CL, Hernday AD, Nobile CJ
  TITLE     A Markerless CRISPR-Mediated System for Genome Editing in Candida 
            auris Reveals a Conserved Role for Cas5 in the Caspofungin Response.
  JOURNAL   Microbiol Spectr. 2021 Dec 22;9(3):e0182021. doi: 
            10.1128/Spectrum.01820-21. Epub 2021 Nov 3.
  PUBMED    34730409
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            //gt12365.cn
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr."; date: "2021-12-22"; volume: "9(3):e0182021. doi"; pages: "
            10.1128/Spectrum.01820-21. Epub 2021 Nov 3"
COMMENT     Sequence Label: pCE35
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(184..288)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     356..374
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             1962..6065
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1962..6065
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6078..6098
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6102..6122
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6126..6146
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6147..6212
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      6277..6524
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6293..6311)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        6564..6907
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        complement(join(7415..7421,7422..7439,7440..7445))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7460..7481
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7598..7615)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7769..8357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7849..7868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8528..9205,1..114,115..183))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9151..9170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
       61 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
      121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
      181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
      241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
      301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
      361 gcgtatcacg aggccctttg acggcacggc cacgcgttta aaccgccttt tcaaatggtt
      421 cagaaaaata ttgttcttct tcctggtgac cacgtgggca ccgaggttgt aaacgaggcc
      481 gtcaaggttt tgaaagccat tgaggaagca acgtctcaag atatcaaatt caatttttca
      541 acccatctaa ttggtggtgc agccattgac aagaccggag tgcctttgcc cgatgaagcc
      601 ctcgaggctg ccaaaaagtc agacgctgtc ttgttgggag ccgttggtgg ccccaaatgg
      661 ggtactggtg ccgttagacc agagcagggt cttctcaaga tcagaaagga attgggcctt
      721 tatgcgaact tgagaccttg caactttgca tctgactcgc ttttggacct ttctccattg
      781 aaggccagtg ttgtcaaagg aaccaatttc accgtggtgc gtgaattggt cggtggtatc
      841 tactttggtg agagacaaga acaggaagag tctgccgacg gtcagagcgc ttgggacacc
      901 gagaagtaca gtgtctctga agttgaaaga atcaccagaa tggctgcctt catggctttg
      961 cagcacaacc ctcctttgcc catctggtcc ttagacaagg ccaacgtgct tgcctcatct
     1021 agattgtgga gaaagacggt cgacaaggtg atgagcgagg agttccctca attgaccatc
     1081 aaccaccagc tcattgactc tgctgccatg attttggtgc agagcccaac taaattgaac
     1141 ggtgttgtga tcacgtcaaa catgaccaat cacaagactt acctgaagac ctgactcaac
     1201 gcaatcgcca gtcccgggtt tcctggctca cgggcccctc cactacgctg atattttgtc
     1261 aattaaccag gagtctaccc tagtgcgcat tgtcgaaggg agtggaaagc cagggaaaaa
     1321 aaacgtatag tgcggtcccc gtcgacccga gacgacaagg cacccaggtg agtcaggctc
     1381 aaatgcatga aaaattatga aaagtggaaa atcgatggcc ctcaggtgaa ggttatcaaa
     1441 acctcatcag ctcgtcgctc aattgctaaa aatcaactga aacagcgtcc cagtggtgtc
     1501 accccacggt tgtttcaggg ctgcggtgga tcacatggcg actccctttg gcccgtaggg
     1561 tgcgatccgt cagtgcccgg ggccctctgc caggattcta cgcgcattgc aactccgagg
     1621 ccgataccct ttgaccagtg gtcaattcgt cacaattggc aacattttca tgatttggca
     1681 gcttcacgtt gaacaattga accagcagaa tccaggaaac gagtttccgt tgtgaatgac
     1741 cctttgtgtg tgcaaataat catggtggct ccctaatggc ataccctcag tcacctggag
     1801 gaattgctcg ctcgagtctc ctgaggtgtc ctcctgcctg tggaaaaagc ggccaggtgt
     1861 ggtccaccgt tggaacagct tgctgtataa ataccacagg tgcgcacgcc ccaggggcct
     1921 tttttttttt ttctcctccc tatccaaact taattttcat catggataaa aagtatagta
     1981 ttggtttaga tattggtact aactctgtgg gttgggcagt tatcactgac gaatataaag
     2041 ttccatcaaa gaaatttaag gtgttaggta acactgacag acactcaata aaaaagaatc
     2101 ttatcggtgc tcttttgttc gactccggtg aaactgccga ggctacacgt ttaaaaagaa
     2161 cagcaagaag aagatatacc cgtagaaaaa atagaatatg ttatttacaa gaaatctttt
     2221 ctaatgaaat ggctaaagtt gatgattcct ttttccatag attggaagag tcatttttgg
     2281 ttgaagaaga caaaaagcat gagagacatc caatctttgg gaatatagtt gatgaagtgg
     2341 cttaccatga aaaatatcct accatttatc atttaagaaa gaaattggta gattcaactg
     2401 ataaagctga ccttagatta atctatttag cacttgccca tatgattaaa tttagaggtc
     2461 attttttgat tgaaggtgat ttgaacccag ataattctga cgtggataaa ttatttattc
     2521 aattagtcca aacctacaac caattatttg aggaaaatcc aattaatgct agtggtgtcg
     2581 atgccaaagc tatattatca gccagattat caaaatctag acgtttggaa aatttgattg
     2641 cccaattgcc aggagaaaaa aagaatggat tatttggaaa cttgatcgca ttatcattgg
     2701 gtttgacacc aaattttaaa tctaattttg atttagctga agatgctaaa ttacaattat
     2761 caaaagacac ctatgacgac gatttggaca atttacttgc tcaaattggt gatcaatatg
     2821 cagatttgtt cttagctgct aaaaacttat ctgatgctat tttgttgtct gatattttga
     2881 gagtgaacac agaaataacc aaagctccat tatcagcatc tatgatcaaa cgttatgatg
     2941 aacaccatca ggatttgact ttattgaaag ctttggtgag acaacaattg ccagagaagt
     3001 ataaagaaat ctttttcgat caatctaaaa acgggtatgc aggttatatt gatgggggtg
     3061 cctcccaaga ggaattttac aaatttataa aacctatttt agaaaagatg gatgggactg
     3121 aggaactttt ggtcaaattg aacagagaag atttgttacg taaacagaga acttttgata
     3181 atggtagtat acctcaccaa attcatttgg gtgagttgca tgcaatttta agaagacaag
     3241 aagattttta tccattttta aaagataata gagaaaaaat cgagaaaatt ttaaccttta
     3301 gaattccata ctatgttggg cctttggcta gaggtaattc aagatttgcc tggatgacac
     3361 gtaaatcaga agaaactatt accccttgga attttgaaga ggttgttgat aaaggagcat
     3421 cagcacagag ttttattgaa agaatgacca atttcgataa aaacttacca aatgaaaaag
     3481 ttttaccaaa acattccttg ttatacgaat attttactgt ttacaatgaa cttacaaagg
     3541 ttaaatatgt tactgaaggt atgcgtaagc cagccttttt atctggagaa cagaaaaagg
     3601 caatagttga tttattgttt aaaacaaata gaaaagttac tgttaaacaa ttaaaagaag
     3661 attactttaa gaaaattgaa tgttttgatt cagttgaaat cagtggtgtt gaagacagat
     3721 ttaatgctag tttaggaact taccatgatt tacttaaaat tatcaaagat aaagatttct
     3781 tggataacga agaaaatgaa gacattttag aagacattgt tttaacctta actttattcg
     3841 aagatagaga gatgattgaa gaacgtttga agacttatgc acatttgttt gacgataaag
     3901 tgatgaaaca gttgaaaaga agacgttata ctggatgggg tagattgtct cgtaaattga
     3961 tcaatggaat tagagataaa caaagtggta aaactatctt ggactttttg aaatctgacg
     4021 gatttgctaa tagaaatttc atgcaattga tccacgacga tagtttgaca tttaaagaag
     4081 acatccaaaa ggcccaagtg agtgggcaag gtgattcatt acatgaacat attgcaaatt
     4141 tagccggatc tcctgctatt aagaaaggga tattacaaac tgttaaagtt gtggatgaat
     4201 tagtgaaagt aatgggaaga cataaacctg aaaacattgt cattgagatg gcaagagaaa
     4261 atcaaactac acaaaaagga cagaaaaata gtagagaacg tatgaaaaga atagaagagg
     4321 gtattaaaga attgggtagt caaatattga aagaacaccc agtggaaaat acccagttgc
     4381 aaaatgaaaa attatatctt tactaccttc aaaatggacg tgatatgtat gttgatcagg
     4441 aattagatat aaatagactt tcagattatg atgtagatca tatagttcca caatctttct
     4501 tgaaagatga ttccatagac aataaagtat taactagaag tgataaaaat agaggtaaaa
     4561 gtgataatgt cccaagtgag gaagtcgtca aaaagatgaa aaattactgg cgtcaacttt
     4621 tgaatgctaa attaattact caaagaaaat ttgataattt gactaaagca gaaagaggtg
     4681 ggctttctga attagataaa gccgggttca ttaaaagaca attggtcgaa actagacaaa
     4741 ttactaaaca tgttgcccaa attttagatt cccgtatgaa cactaagtat gacgaaaatg
     4801 ataagttaat acgtgaggtt aaagtcatta ctttaaaatc aaaacttgtc tctgatttca
     4861 gaaaggattt ccaattctat aaagttagag aaattaataa ttatcatcat gctcatgatg
     4921 catatttgaa tgctgtagtt ggaactgctt taatcaagaa ataccctaaa ttagaatctg
     4981 aatttgtata tggtgattac aaagtctatg atgttagaaa gatgattgct aaatcagaac
     5041 aagaaattgg taaagctaca gctaaatact tcttttactc taacattatg aatttcttta
     5101 aaacagaaat tactttggca aacggtgaaa ttagaaaaag acctcttatt gaaacaaatg
     5161 gtgagactgg agagatagtt tgggacaaag ggcgtgattt cgctactgtt agaaaagttt
     5221 tatcaatgcc acaagttaac attgtaaaga aaacagaggt tcaaactggt ggtttctcaa
     5281 aagaaagtat tttgcctaaa agaaatagtg ataaattgat tgccagaaaa aaggattggg
     5341 atccaaagaa atatggtggt ttcgactcac caaccgtagc ctattctgtt ttggttgtgg
     5401 caaaggttga aaagggtaaa agtaaaaagc ttaaatcagt aaaagaactt ttgggtatta
     5461 caataatgga aagaagttcc tttgaaaaga accctattga ttttttggaa gctaaaggtt
     5521 ataaggaagt aaagaaggac ttaataatca aattgcctaa atattcttta tttgaattag
     5581 aaaatgggag aaaaagaatg ttggcttctg ctggagaatt gcaaaagggt aatgaattag
     5641 cattgccttc caaatatgtt aacttcttgt atttagcttc acactatgaa aagttgaaag
     5701 ggtcaccaga agataacgag caaaaacaat tatttgttga acaacacaaa cactacttag
     5761 atgagattat agaacaaatt agtgaattca gtaaaagagt gatattagct gatgcaaatt
     5821 tagataaagt tttgtcagcc tataacaaac atagagataa gccaattaga gaacaagcag
     5881 aaaacattat tcacttattt acccttacca atttaggagc acctgctgct ttcaagtatt
     5941 ttgatacaac aattgatcgt aaaagatata cctcaacaaa agaagtctta gacgccacct
     6001 taattcatca atcaatcact ggattgtatg agacaagaat tgatttgtct caattgggtg
     6061 gtgatgaagg ggctgatcct aagaagaaaa gaaaagttga tccaaagaaa aagcgtaagg
     6121 tggatcctaa gaaaaagaga aaggttgact acaaagacca tgacggtgat tataaagatc
     6181 atgacatcga ctacaaggat gacgatgaca agtgataatg actgcagaga tccatcgacc
     6241 tgccgccaag ctaattccgg gcgaatttct gtcgagtcat gtaattagtt atgtcacgct
     6301 tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg
     6361 aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat
     6421 ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa
     6481 ccttgcttta gaaggttttg ggacgctcga aggctttaat ttgcggccag cttgcctcgt
     6541 ccccgcctgg tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg
     6601 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc
     6661 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg
     6721 gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc
     6781 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct
     6841 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata
     6901 aacaaccgga tcctacaatt atgtctacta ctttggatga tactgcttat agatacagaa
     6961 cttctgttcc aggtgatgct gaagctattg aagctttgga tggttctttc actaccgata
     7021 ctgtttttag agttactgct actggtgatg gtttcacttt gagagaagtt ccagttgatc
     7081 caccattgac taaggttttc ccagatgatg aatccgatga tgaatccgat gctggtgaag
     7141 atggtgatcc agattctaga actttcgttg cttatggtga tgatggtgat ttggctggtt
     7201 tcgttgttgt ttcttattct ggttggaaca gaagattgac tgttgaagat attgaagttg
     7261 ctccagaaca tagaggtcat ggtgttggta gagctttgat gggtttggct actgaattcg
     7321 ccagagaaag aggtgctggt catttgtggt tggaagttac caatgttaat cggtgtttaa
     7381 accccagcgc ctggcgggcg ctcacaattc cacacaacat acgagccgga agcataaagt
     7441 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7501 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7561 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7621 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7681 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7741 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7801 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7861 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7921 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7981 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8041 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8101 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8161 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8221 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8281 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8341 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8401 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8461 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8521 ctgacagtta ccaatgcttg atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8581 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8641 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8701 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8761 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8821 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8881 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8941 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9001 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9061 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9121 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9181 aagtgctcat cattggaaaa cgttc
//