LOCUS       pDEST53-LRRK2-G2019S      13749 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13749)
  AUTHORS   Greggio E, Jain S, Kingsbury A, Bandopadhyay R, Lewis P, Kaganovich 
            A, van der Brug MP, Beilina A, Blackinton J, Thomas KJ, Ahmad R, 
            Miller DW, Kesavapany S, Singleton A, Lees A, Harvey RJ, Harvey K, 
            Cookson MR
  TITLE     Kinase activity is required for the toxic effects of mutant 
            LRRK2/dardarin.
  JOURNAL   Neurobiol Dis. 2006 Aug . 23(2):329-41.
  PUBMED    16750377
REFERENCE   2  (bases 1 to 13749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            //gt12365.cn
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurobiol 
            Dis. 2006 Aug . 23(2):329-41."
COMMENT     Sequence Label: pDEST53-LRRK2-G2019S
FEATURES             Location/Qualifiers
     source          1..13749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(66..90)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(122..139)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    128..352
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      398..826
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(485..504)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     695..716
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(835..855)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        840..1169
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1020..1155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1082..1101
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1236..2030
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1290..1309)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1900..1919
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2204..2325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2241..2260)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2295..2314
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2374..2390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2374..2390)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2387..2409)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2398..2414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2422..2428,2429..2446,2447..2452))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2467..2488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2605..2622)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2776..3364)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2856..2875)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3535..4326,4327..4395))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4158..4177
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4396..4500)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4575..4594)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4766..5145
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5146..5349
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5300..5320
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5394..5413
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5394..5412
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(5436..5438,5439..5441,5442..6152)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(5469..5497)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6092..6112
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    6174..6198
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgagaaaaga attagctgaa aaaatgagac gaacatctgt tgagtaagag agaagggcga
       61 attcgaccca gctttcttgt acaaagtggt gataattaat taagataaac ccgctgatca
      121 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
      181 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
      241 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
      301 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
      361 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
      421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      541 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
      781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
      841 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      901 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
      961 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     1021 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     1081 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     1141 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     1201 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     1261 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     1321 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     1381 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     1441 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     1501 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     1561 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     1621 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     1681 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     1741 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     1801 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     1861 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     1921 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     1981 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     2041 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     2101 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     2161 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     2221 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     2281 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     2341 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     2401 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     2461 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     2521 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2581 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2641 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     2701 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2761 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2821 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2881 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2941 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3001 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3061 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3121 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3181 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3241 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3301 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3361 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3421 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3481 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3541 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3601 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3661 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     3721 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     3781 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     3841 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     3901 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     3961 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4021 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4081 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4141 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4201 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4261 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4321 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4381 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4441 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4501 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     4561 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     4621 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     4681 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     4741 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     4801 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     4861 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     4921 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     4981 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     5041 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     5101 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     5161 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     5221 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     5281 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     5341 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
     5401 actcactata gggagaccca agctggctag acaccatggc cagcaaagga gaagaacttt
     5461 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
     5521 ctgtcagtgg agagggtgaa ggtgatgcta catacggaaa gcttaccctt aaatttattt
     5581 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc tcttatggtg
     5641 ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc aagagtgcca
     5701 tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg aactacaaga
     5761 cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag ttaaaaggta
     5821 ttgattttaa agaagatgga aacattctcg gacacaaact cgagtacaac tataactcac
     5881 acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac ttcaaaattc
     5941 gccacaacat tgaagatgga tccgttcaac tagcagacca ttatcaacaa aatactccaa
     6001 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa tctgcccttt
     6061 cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta actgctgctg
     6121 ggattacaca tggcatggat gagctctaca aaagcggttc cggaccggat caaacaagtt
     6181 tgtacaaaaa agcaggctcc gaattcgccc ttatggctag tggcagctgt caggggtgcg
     6241 aagaggacga ggaaactctg aagaagttga tagtcaggct gaacaatgtc caggaaggaa
     6301 aacagataga aacgctggtc caaatcctgg aggatctgct ggtgttcacg tactccgagc
     6361 acgcctccaa gttatttcaa ggcaaaaata tccatgtgcc tctgttgatc gtcttggact
     6421 cctatatgag agtcgcgagt gtgcagcagg tgggttggtc acttctgtgc aaattaatag
     6481 aagtctgtcc aggtacaatg caaagcttaa tgggacccca ggatgttgga aatgattggg
     6541 aagtccttgg tgttcaccaa ttgattctta aaatgctaac agttcataat gccagtgtaa
     6601 acttgtcagt gattggactg aagaccttag atctcctcct aacttcaggt aaaatcacct
     6661 tgctgatatt ggatgaagaa agtgatattt tcatgttaat ttttgatgcc atgcactcat
     6721 ttccagccaa tgatgaagtc cagaaacttg gatgcaaagc tttacatgtg ctgtttgaga
     6781 gagtctcaga ggagcaactg actgaatttg ttgagaacaa agattatatg atattgttaa
     6841 gtgcgttaac aaattttaaa gatgaagagg aaattgtgct tcatgtgctg cattgtttac
     6901 attccctagc gattccttgc aataatgtgg aagtcctcat gagtggcaat gtcaggtgtt
     6961 ataatattgt ggtggaagct atgaaagcat tccctatgag tgaaagaatt caagaagtga
     7021 gttgctgttt gctccatagg cttacattag gtaatttttt caatatcctg gtattaaacg
     7081 aagtccatga gtttgtggtg aaagctgtgc agcagtaccc agagaatgca gcattgcaga
     7141 tctcagcgct cagctgtttg gccctcctca ctgagactat tttcttaaat caagatttag
     7201 aggaaaagaa tgagaatcaa gagaatgatg atgaggggga agaagataaa ttgttttggc
     7261 tggaagcctg ttacaaagca ttaacgtggc atagaaagaa caagcacgtg caggaggccg
     7321 catgctgggc actaaataat ctccttatgt accaaaacag tttacatgag aagattggag
     7381 atgaagatgg ccatttccca gctcataggg aagtgatgct ctccatgctg atgcattctt
     7441 catcaaagga agttttccag gcatctgcga atgcattgtc aactctctta gaacaaaatg
     7501 ttaatttcag aaaaatactg ttatcaaaag gaatacacct gaatgttttg gagttaatgc
     7561 agaagcatat acattctccc gaagtggctg aaagtggctg taaaatgcta aatcatcttt
     7621 ttgaaggaag caacacttcc ctggatataa tggcagcagt ggtccccaaa atactaacag
     7681 ttatgaaacg tcatgagaca tcattaccag tgcagctgga ggcgcttcga gctattttac
     7741 attttatagt gcctggcatg ccagaagaat ccagggagga tacagaattt catcataagc
     7801 taaatatggt taaaaaacag tgtttcaaga atgatattca caaactggtc ctagcagctt
     7861 tgaacaggtt cattggaaat cctgggattc agaaatgtgg attaaaagta atttcttcta
     7921 ttgtacattt tcctgatgca ttagagatgt tatccctgga aggtgctatg gattcagtgc
     7981 ttcacacact gcagatgtat ccagatgacc aagaaattca gtgtctgggt ttaagtctta
     8041 taggatactt gattacaaag aagaatgtgt tcataggaac tggacatctg ctggcaaaaa
     8101 ttctggtttc cagcttatac cgatttaagg atgttgctga aatacagact aaaggatttc
     8161 agacaatctt agcaatcctc aaattgtcag catctttttc taagctgctg gtgcatcatt
     8221 catttgactt agtaatattc catcaaatgt cttccaatat catggaacaa aaggatcaac
     8281 agtttctaaa cctctgttgc aagtgttttg caaaagtagc tatggatgat tacttaaaaa
     8341 atgtgatgct agagagagcg tgtgatcaga ataacagcat catggttgaa tgcttgcttc
     8401 tattgggagc agatgccaat caagcaaagg agggatcttc tttaatttgt caggtatgtg
     8461 agaaagagag cagtcccaaa ttggtggaac tcttactgaa tagtggatct cgtgaacaag
     8521 atgtacgaaa agcgttgacg ataagcattg ggaaaggtga cagccagatc atcagcttgc
     8581 tcttaaggag gctggccctg gatgttgcca acaatagcat ttgccttgga ggattttgta
     8641 taggaaaagt tgaaccttct tggcttggtc ctttatttcc agataagact tctaatttaa
     8701 ggaaacaaac aaatatagca tctacactag caagaatggt gatcagatat cagatgaaaa
     8761 gtgctgtgga agaaggaaca gcctcaggca gcgatggaaa tttttctgaa gatgtgctgt
     8821 ctaaatttga tgaatggacc tttattcctg actcttctat ggacagtgtg tttgctcaaa
     8881 gtgatgacct ggatagtgaa ggaagtgaag gctcatttct tgtgaaaaag aaatctaatt
     8941 caattagtgt aggagaattt taccgagatg ccgtattaca gcgttgctca ccaaatttgc
     9001 aaagacattc caattccttg gggcccattt ttgatcatga agatttactg aagcgaaaaa
     9061 gaaaaatatt atcttcagat gattcactca ggtcatcaaa acttcaatcc catatgaggc
     9121 attcagacag catttcttct ctggcttctg agagagaata tattacatca ctagaccttt
     9181 cagcaaatga actaagagat attgatgccc taagccagaa atgctgtata agtgttcatt
     9241 tggagcatct tgaaaagctg gagcttcacc agaatgcact cacgagcttt ccacaacagc
     9301 tatgtgaaac tctgaagagt ttgacacatt tggacttgca cagtaataaa tttacatcat
     9361 ttccttctta tttgttgaaa atgagttgta ttgctaatct tgatgtctct cgaaatgaca
     9421 ttggaccctc agtggtttta gatcctacag tgaaatgtcc aactctgaaa cagtttaacc
     9481 tgtcatataa ccagctgtct tttgtacctg agaacctcac tgatgtggta gagaaactgg
     9541 agcagctcat tttagaagga aataaaatat cagggatatg ctcccccttg agactgaagg
     9601 aactgaagat tttaaacctt agtaagaacc acatttcatc cctatcagag aactttcttg
     9661 aggcttgtcc taaagtggag agtttcagtg ccagaatgaa ttttcttgct gctatgcctt
     9721 tcttgcctcc ttctatgaca atcctaaaat tatctcagaa caaattttcc tgtattccag
     9781 aagcaatttt aaatcttcca cacttgcggt ctttagatat gagcagcaat gatattcagt
     9841 acctaccagg tcccgcacac tggaaatctt tgaacttaag ggaactctta tttagccata
     9901 atcagatcag catcttggac ttgagtgaaa aagcatattt atggtctaga gtagagaaac
     9961 tgcatctttc tcacaataaa ctgaaagaga ttcctcctga gattggctgt cttgaaaatc
    10021 tgacatctct ggatgtcagt tacaacttgg aactaagatc ctttcccaat gaaatgggga
    10081 aattaagcaa aatatgggat cttcctttgg atgaactgca tcttaacttt gattttaaac
    10141 atataggatg taaagccaaa gacatcataa ggtttcttca acagcgatta aaaaaggctg
    10201 tgccttataa ccgaatgaaa cttatgattg tgggaaatac tgggagtggt aaaaccacct
    10261 tattgcagca attaatgaaa accaagaaat cagatcttgg aatgcaaagt gccacagttg
    10321 gcatagatgt gaaagactgg cctatccaaa taagagacaa aagaaagaga gatctcgtcc
    10381 taaatgtgtg ggattttgca ggtcgtgagg aattctatag tactcatccc cattttatga
    10441 cgcagcgagc attgtacctt gctgtctatg acctcagcaa gggacaggct gaagttgatg
    10501 ccatgaagcc ttggctcttc aatataaagg ctcgcgcttc ttcttcccct gtgattctcg
    10561 ttggcacaca tttggatgtt tctgatgaga agcaacgcaa agcctgcatg agtaaaatca
    10621 ccaaggaact cctgaataag cgagggttcc ctgccatacg agattaccac tttgtgaatg
    10681 ccaccgagga atctgatgct ttggcaaaac ttcggaaaac catcataaac gagagcctta
    10741 atttcaagat ccgagatcag cttgttgttg gacagctgat tccagactgc tatgtagaac
    10801 ttgaaaaaat cattttatcg gagcgtaaaa atgtgccaat tgaatttccc gtaattgacc
    10861 ggaaacgatt attacaacta gtgagagaaa atcagctgca gttagatgaa aatgagcttc
    10921 ctcacgcagt tcactttcta aatgaatcag gagtccttct tcattttcaa gacccagcac
    10981 tgcagttaag tgacttgtac tttgtggaac ccaagtggct ttgtaaaatc atggcacaga
    11041 ttttgacagt gaaagtggaa ggttgtccaa aacaccctaa gggaattatt tcgcgtagag
    11101 atgtggaaaa atttctttca aaaaaaagga aatttccaaa gaactacatg tcacagtatt
    11161 ttaagctcct agaaaaattc cagattgctt tgccaatagg agaagaatat ttgctggttc
    11221 caagcagttt gtctgaccac aggcctgtga tagagcttcc ccattgtgag aactctgaaa
    11281 ttatcatccg actatatgaa atgccttatt ttccaatggg attttggtca agattaatca
    11341 atcgattact tgagatttca ccttacatgc tttcagggag agaacgagca cttcgcccaa
    11401 acagaatgta ttggcgacaa ggcatttact taaattggtc tcctgaagct tattgtctgg
    11461 taggatctga agtcttagac aatcatccag agagtttctt aaaaattaca gttccttctt
    11521 gtagaaaagg ctgtattctt ttgggccaag ttgtggacca cattgattct ctcatggaag
    11581 aatggtttcc tgggttgctg gagattgata tttgtggtga aggagaaact ctgttgaaga
    11641 aatgggcatt atatagtttt aatgatggcg aagaacatca aaaaatctta cttgatgact
    11701 tgatgaagaa agcagaggaa ggagatctct tagtaaatcc agatcaacca aggctcacca
    11761 ttccaatatc tcagattgcc cctgacttga ttttggctga cctgcctaga aatattatgt
    11821 tgaataatga tgagttggaa tttgaacaag ctccagagtt tctcctaggt gatggcagtt
    11881 ttggatcagt ttaccgagca gcctatgaag gagaagaagt ggctgtgaag atttttaata
    11941 aacatacatc actcaggctg ttaagacaag agcttgtggt gctttgccac ctccaccacc
    12001 ccagtttgat atctttgctg gcagctggga ttcgtccccg gatgttggtg atggagttag
    12061 cctccaaggg ttccttggat cgcctgcttc agcaggacaa agccagcctc actagaaccc
    12121 tacagcacag gatcgcactc cacgtagctg atggtttgag atacctccac tcagccatga
    12181 ttatataccg agacctgaaa ccccacaatg tgctgctttt cacactgtat cccaatgctg
    12241 ccatcatcgc aaagattgct gactacagca ttgctcagta ctgctgtaga atggggataa
    12301 aaacatcaga gggcacacca gggtttcgtg cacctgaagt tgccagagga aatgtcattt
    12361 ataaccaaca ggctgatgtt tattcatttg gtttactact ctatgacatt ttgacaactg
    12421 gaggtagaat agtagagggt ttgaagtttc caaatgagtt tgatgaatta gaaatacaag
    12481 gaaaattacc tgatccagtt aaagaatatg gttgtgcccc atggcctatg gttgagaaat
    12541 taattaaaca gtgtttgaaa gaaaatcctc aagaaaggcc tacttctgcc caggtctttg
    12601 acattttgaa ttcagctgaa ttagtctgtc tgacgagacg cattttatta cctaaaaacg
    12661 taattgttga atgcatggtt gctacacatc acaacagcag gaatgcaagc atttggctgg
    12721 gctgtgggca caccgacaga ggacagctct catttcttga cttaaatact gaaggataca
    12781 cttctgagga agttgctgat agtagaatat tgtgcttagc cttggtgcat cttcctgttg
    12841 aaaaggaaag ctggattgtg tctgggacac agtctggtac tctcctggtc atcaataccg
    12901 aagatgggaa aaagagacat accctagaaa agatgactga ttctgtcact tgtttgtatt
    12961 gcaattcctt ttccaagcaa agcaaacaaa aaaattttct tttggttgga accgctgatg
    13021 gcaagttagc aatttttgaa gataagactg ttaagcttaa aggagctgct cctttgaaga
    13081 tactaaatat aggaaatgtc agtactccat tgatgtgttt gagtgaatcc acaaattcaa
    13141 cggaaagaaa tgtaatgtgg ggaggatgtg gcacaaagat tttctccttt tctaatgatt
    13201 tcaccattca gaaactcatt gagacaagaa caagccaact gttttcttat gcagctttca
    13261 gtgattccaa catcataaca gtggtggtag acactgctct ctatattgct aagcaaaata
    13321 gccctgttgt ggaagtgtgg gataagaaaa ctgaaaaact ctgtggacta atagactgcg
    13381 tgcacttttt aagggaggta atggtaaaag aaaacaagga atcaaaacac aaaatgtctt
    13441 attctgggag agtgaaaacc ctctgccttc agaagaacac tgctctttgg ataggaactg
    13501 gaggaggcca tattttactc ctggatcttt caactcgtcg acttatacgt gtaatttaca
    13561 acttttgtaa ttcggtcaga gtcatgatga cagcacagct aggaagcctt aaaaatgtca
    13621 tgctggtatt gggctacaac cggaaaaata ctgaaggtac acaaaagcag aaagagatac
    13681 aatcttgctt gaccgtttgg gacatcaatc ttccacatga agtgcaaaat ttagaaaaac
    13741 acattgaag
//