| 質粒名稱: | pDEST53-LRRK2-WT |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 25044 |
| 存儲實驗室: | Mark Cookson |
|
載體骨架基本信息 |
|
| 載體名稱: | pcDNA-DEST53 |
| 載體出品公司: | Invitrogen |
| 空載體大小: | 7767 bp |
| 完整質粒大小: | 13764 bp |
| 載體類型: | 哺乳細胞表達 |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | LRRK2 |
| 物種來源: | H. sapiens (human) |
| 插入大小: | 7584 bp |
| 標簽/融合蛋白: | GFP (N terminal on insert) |
| 克隆方法: | Gateway Cloning |
| 5’克隆位點: | attR1 (not destroyed) |
| 3’克隆位點: | attR2 (not destroyed) |
| 5’測序引物 | CMV-F, GFP-F |
| 3’測序引物 | BGH-rev |
|
文獻引用方法 |
|
| 材料和方法部分: | pDEST53-LRRK2-WT was a gift from Mark Cookson (Addgene plasmid # 25044 ; //n2t.net/addgene:25044 ; RRID:Addgene_25044) |
| 參考文獻部分: | Kinase activity is required for the toxic effects of mutant LRRK2/dardarin. Greggio E, Jain S, Kingsbury A, Bandopadhyay R, Lewis P, Kaganovich A, van der Brug MP, Beilina A, Blackinton J, Thomas KJ, Ahmad R, Miller DW, Kesavapany S, Singleton A, Lees A, Harvey RJ, Harvey K, Cookson MR. Neurobiol Dis. 2006 Aug . 23(2):329-41. 10.1016/j.nbd.2006.04.001 PubMed 16750377 |
買家導航
pDEST53-LRRK2-WT載體質粒基本信息
pDEST53-LRRK2-WT質粒圖譜載體圖譜和pDEST53-LRRK2-WT載體序列質粒序列多克隆位點信息
pDEST53-LRRK2-WT質粒載體簡介
pDEST53-LRRK2-WT質粒序列載體序列
LOCUS pDEST53-LRRK2-WT 13749 bp DNA circular SYN 14-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13749)
AUTHORS Greggio E, Jain S, Kingsbury A, Bandopadhyay R, Lewis P, Kaganovich
A, van der Brug MP, Beilina A, Blackinton J, Thomas KJ, Ahmad R,
Miller DW, Kesavapany S, Singleton A, Lees A, Harvey RJ, Harvey K,
Cookson MR
TITLE Kinase activity is required for the toxic effects of mutant
LRRK2/dardarin.
JOURNAL Neurobiol Dis. 2006 Aug . 23(2):329-41.
PUBMED 16750377
REFERENCE 2 (bases 1 to 13749)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Neurobiol
Dis. 2006 Aug . 23(2):329-41."
COMMENT Sequence Label: pDEST53-LRRK2-WT
FEATURES Location/Qualifiers
source 1..13749
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 112..906
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(166..185)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 776..795
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 1080..1201
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1117..1136)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1171..1190
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(1250..1266)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1250..1266)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1263..1285)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 1274..1290
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(1298..1304,1305..1322,1323..1328))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1343..1364
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(1481..1498)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1652..2240)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1732..1751)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(2411..3202,3203..3271))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3034..3053
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(3272..3376)
/gene="bla"
/label=AmpR promoter
primer_bind complement(3451..3470)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 3642..4021
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4022..4225
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 4176..4196
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 4270..4289
/label=T7
/note="T7 promoter, forward primer"
promoter 4270..4288
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS join(4312..4314,4315..4317,4318..5028)
/codon_start=1
/product="Cycle 3 GFP (Crameri et al., 1996)"
/label=Cycle 3 GFP
/note="mammalian codon-optimized"
/translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
primer_bind complement(4345..4373)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 4968..4988
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
protein_bind 5050..5074
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(12691..12715)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
primer_bind complement(12747..12764)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 12753..12977
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 13023..13451
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(13110..13129)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 13320..13341
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(13460..13480)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter join(13465..13749,1..45)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin join(13645..13749,1..31)
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 13707..13726
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
ORIGIN
1 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
61 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
121 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
181 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
241 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
301 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
361 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
421 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
481 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
541 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
601 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
661 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
721 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
781 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
841 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
901 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
961 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
1021 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
1081 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
1141 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
1201 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
1261 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
1321 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
1381 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
1441 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
1501 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
1561 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
1621 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
1681 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
1741 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
1801 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
1861 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
1921 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
1981 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
2041 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
2101 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
2161 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
2221 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
2281 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
2341 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
2401 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
2461 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
2521 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
2581 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
2641 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
2701 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
2761 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
2821 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
2881 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
2941 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
3001 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
3061 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
3121 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
3181 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
3241 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
3301 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
3361 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
3421 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
3481 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
3541 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
3601 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
3661 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
3721 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
3781 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
3841 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
3901 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
3961 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
4021 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
4081 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
4141 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
4201 gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg cttactggct
4261 tatcgaaatt aatacgactc actataggga gacccaagct ggctagacac catggccagc
4321 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
4381 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgctacata cggaaagctt
4441 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
4501 actttctctt atggtgttca atgcttttcc cgttatccgg atcatatgaa acggcatgac
4561 tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat
4621 gacgggaact acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt
4681 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttctcggaca caaactcgag
4741 tacaactata actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa
4801 gctaacttca aaattcgcca caacattgaa gatggatccg ttcaactagc agaccattat
4861 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg
4921 acacaatctg ccctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag
4981 tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaaag cggttccgga
5041 ccggatcaaa caagtttgta caaaaaagca ggctccgaat tcgcccttat ggctagtggc
5101 agctgtcagg ggtgcgaaga ggacgaggaa actctgaaga agttgatagt caggctgaac
5161 aatgtccagg aaggaaaaca gatagaaacg ctggtccaaa tcctggagga tctgctggtg
5221 ttcacgtact ccgagcacgc ctccaagtta tttcaaggca aaaatatcca tgtgcctctg
5281 ttgatcgtct tggactccta tatgagagtc gcgagtgtgc agcaggtggg ttggtcactt
5341 ctgtgcaaat taatagaagt ctgtccaggt acaatgcaaa gcttaatggg accccaggat
5401 gttggaaatg attgggaagt ccttggtgtt caccaattga ttcttaaaat gctaacagtt
5461 cataatgcca gtgtaaactt gtcagtgatt ggactgaaga ccttagatct cctcctaact
5521 tcaggtaaaa tcaccttgct gatattggat gaagaaagtg atattttcat gttaattttt
5581 gatgccatgc actcatttcc agccaatgat gaagtccaga aacttggatg caaagcttta
5641 catgtgctgt ttgagagagt ctcagaggag caactgactg aatttgttga gaacaaagat
5701 tatatgatat tgttaagtgc gttaacaaat tttaaagatg aagaggaaat tgtgcttcat
5761 gtgctgcatt gtttacattc cctagcgatt ccttgcaata atgtggaagt cctcatgagt
5821 ggcaatgtca ggtgttataa tattgtggtg gaagctatga aagcattccc tatgagtgaa
5881 agaattcaag aagtgagttg ctgtttgctc cataggctta cattaggtaa ttttttcaat
5941 atcctggtat taaacgaagt ccatgagttt gtggtgaaag ctgtgcagca gtacccagag
6001 aatgcagcat tgcagatctc agcgctcagc tgtttggccc tcctcactga gactattttc
6061 ttaaatcaag atttagagga aaagaatgag aatcaagaga atgatgatga gggggaagaa
6121 gataaattgt tttggctgga agcctgttac aaagcattaa cgtggcatag aaagaacaag
6181 cacgtgcagg aggccgcatg ctgggcacta aataatctcc ttatgtacca aaacagttta
6241 catgagaaga ttggagatga agatggccat ttcccagctc atagggaagt gatgctctcc
6301 atgctgatgc attcttcatc aaaggaagtt ttccaggcat ctgcgaatgc attgtcaact
6361 ctcttagaac aaaatgttaa tttcagaaaa atactgttat caaaaggaat acacctgaat
6421 gttttggagt taatgcagaa gcatatacat tctcccgaag tggctgaaag tggctgtaaa
6481 atgctaaatc atctttttga aggaagcaac acttccctgg atataatggc agcagtggtc
6541 cccaaaatac taacagttat gaaacgtcat gagacatcat taccagtgca gctggaggcg
6601 cttcgagcta ttttacattt tatagtgcct ggcatgccag aagaatccag ggaggataca
6661 gaatttcatc ataagctaaa tatggttaaa aaacagtgtt tcaagaatga tattcacaaa
6721 ctggtcctag cagctttgaa caggttcatt ggaaatcctg ggattcagaa atgtggatta
6781 aaagtaattt cttctattgt acattttcct gatgcattag agatgttatc cctggaaggt
6841 gctatggatt cagtgcttca cacactgcag atgtatccag atgaccaaga aattcagtgt
6901 ctgggtttaa gtcttatagg atacttgatt acaaagaaga atgtgttcat aggaactgga
6961 catctgctgg caaaaattct ggtttccagc ttataccgat ttaaggatgt tgctgaaata
7021 cagactaaag gatttcagac aatcttagca atcctcaaat tgtcagcatc tttttctaag
7081 ctgctggtgc atcattcatt tgacttagta atattccatc aaatgtcttc caatatcatg
7141 gaacaaaagg atcaacagtt tctaaacctc tgttgcaagt gttttgcaaa agtagctatg
7201 gatgattact taaaaaatgt gatgctagag agagcgtgtg atcagaataa cagcatcatg
7261 gttgaatgct tgcttctatt gggagcagat gccaatcaag caaaggaggg atcttcttta
7321 atttgtcagg tatgtgagaa agagagcagt cccaaattgg tggaactctt actgaatagt
7381 ggatctcgtg aacaagatgt acgaaaagcg ttgacgataa gcattgggaa aggtgacagc
7441 cagatcatca gcttgctctt aaggaggctg gccctggatg ttgccaacaa tagcatttgc
7501 cttggaggat tttgtatagg aaaagttgaa ccttcttggc ttggtccttt atttccagat
7561 aagacttcta atttaaggaa acaaacaaat atagcatcta cactagcaag aatggtgatc
7621 agatatcaga tgaaaagtgc tgtggaagaa ggaacagcct caggcagcga tggaaatttt
7681 tctgaagatg tgctgtctaa atttgatgaa tggaccttta ttcctgactc ttctatggac
7741 agtgtgtttg ctcaaagtga tgacctggat agtgaaggaa gtgaaggctc atttcttgtg
7801 aaaaagaaat ctaattcaat tagtgtagga gaattttacc gagatgccgt attacagcgt
7861 tgctcaccaa atttgcaaag acattccaat tccttggggc ccatttttga tcatgaagat
7921 ttactgaagc gaaaaagaaa aatattatct tcagatgatt cactcaggtc atcaaaactt
7981 caatcccata tgaggcattc agacagcatt tcttctctgg cttctgagag agaatatatt
8041 acatcactag acctttcagc aaatgaacta agagatattg atgccctaag ccagaaatgc
8101 tgtataagtg ttcatttgga gcatcttgaa aagctggagc ttcaccagaa tgcactcacg
8161 agctttccac aacagctatg tgaaactctg aagagtttga cacatttgga cttgcacagt
8221 aataaattta catcatttcc ttcttatttg ttgaaaatga gttgtattgc taatcttgat
8281 gtctctcgaa atgacattgg accctcagtg gttttagatc ctacagtgaa atgtccaact
8341 ctgaaacagt ttaacctgtc atataaccag ctgtcttttg tacctgagaa cctcactgat
8401 gtggtagaga aactggagca gctcatttta gaaggaaata aaatatcagg gatatgctcc
8461 cccttgagac tgaaggaact gaagatttta aaccttagta agaaccacat ttcatcccta
8521 tcagagaact ttcttgaggc ttgtcctaaa gtggagagtt tcagtgccag aatgaatttt
8581 cttgctgcta tgcctttctt gcctccttct atgacaatcc taaaattatc tcagaacaaa
8641 ttttcctgta ttccagaagc aattttaaat cttccacact tgcggtcttt agatatgagc
8701 agcaatgata ttcagtacct accaggtccc gcacactgga aatctttgaa cttaagggaa
8761 ctcttattta gccataatca gatcagcatc ttggacttga gtgaaaaagc atatttatgg
8821 tctagagtag agaaactgca tctttctcac aataaactga aagagattcc tcctgagatt
8881 ggctgtcttg aaaatctgac atctctggat gtcagttaca acttggaact aagatccttt
8941 cccaatgaaa tggggaaatt aagcaaaata tgggatcttc ctttggatga actgcatctt
9001 aactttgatt ttaaacatat aggatgtaaa gccaaagaca tcataaggtt tcttcaacag
9061 cgattaaaaa aggctgtgcc ttataaccga atgaaactta tgattgtggg aaatactggg
9121 agtggtaaaa ccaccttatt gcagcaatta atgaaaacca agaaatcaga tcttggaatg
9181 caaagtgcca cagttggcat agatgtgaaa gactggccta tccaaataag agacaaaaga
9241 aagagagatc tcgtcctaaa tgtgtgggat tttgcaggtc gtgaggaatt ctatagtact
9301 catccccatt ttatgacgca gcgagcattg taccttgctg tctatgacct cagcaaggga
9361 caggctgaag ttgatgccat gaagccttgg ctcttcaata taaaggctcg cgcttcttct
9421 tcccctgtga ttctcgttgg cacacatttg gatgtttctg atgagaagca acgcaaagcc
9481 tgcatgagta aaatcaccaa ggaactcctg aataagcgag ggttccctgc catacgagat
9541 taccactttg tgaatgccac cgaggaatct gatgctttgg caaaacttcg gaaaaccatc
9601 ataaacgaga gccttaattt caagatccga gatcagcttg ttgttggaca gctgattcca
9661 gactgctatg tagaacttga aaaaatcatt ttatcggagc gtaaaaatgt gccaattgaa
9721 tttcccgtaa ttgaccggaa acgattatta caactagtga gagaaaatca gctgcagtta
9781 gatgaaaatg agcttcctca cgcagttcac tttctaaatg aatcaggagt ccttcttcat
9841 tttcaagacc cagcactgca gttaagtgac ttgtactttg tggaacccaa gtggctttgt
9901 aaaatcatgg cacagatttt gacagtgaaa gtggaaggtt gtccaaaaca ccctaaggga
9961 attatttcgc gtagagatgt ggaaaaattt ctttcaaaaa aaaggaaatt tccaaagaac
10021 tacatgtcac agtattttaa gctcctagaa aaattccaga ttgctttgcc aataggagaa
10081 gaatatttgc tggttccaag cagtttgtct gaccacaggc ctgtgataga gcttccccat
10141 tgtgagaact ctgaaattat catccgacta tatgaaatgc cttattttcc aatgggattt
10201 tggtcaagat taatcaatcg attacttgag atttcacctt acatgctttc agggagagaa
10261 cgagcacttc gcccaaacag aatgtattgg cgacaaggca tttacttaaa ttggtctcct
10321 gaagcttatt gtctggtagg atctgaagtc ttagacaatc atccagagag tttcttaaaa
10381 attacagttc cttcttgtag aaaaggctgt attcttttgg gccaagttgt ggaccacatt
10441 gattctctca tggaagaatg gtttcctggg ttgctggaga ttgatatttg tggtgaagga
10501 gaaactctgt tgaagaaatg ggcattatat agttttaatg atggcgaaga acatcaaaaa
10561 atcttacttg atgacttgat gaagaaagca gaggaaggag atctcttagt aaatccagat
10621 caaccaaggc tcaccattcc aatatctcag attgcccctg acttgatttt ggctgacctg
10681 cctagaaata ttatgttgaa taatgatgag ttggaatttg aacaagctcc agagtttctc
10741 ctaggtgatg gcagttttgg atcagtttac cgagcagcct atgaaggaga agaagtggct
10801 gtgaagattt ttaataaaca tacatcactc aggctgttaa gacaagagct tgtggtgctt
10861 tgccacctcc accaccccag tttgatatct ttgctggcag ctgggattcg tccccggatg
10921 ttggtgatgg agttagcctc caagggttcc ttggatcgcc tgcttcagca ggacaaagcc
10981 agcctcacta gaaccctaca gcacaggatc gcactccacg tagctgatgg tttgagatac
11041 ctccactcag ccatgattat ataccgagac ctgaaacccc acaatgtgct gcttttcaca
11101 ctgtatccca atgctgccat catcgcaaag attgctgact acggcattgc tcagtactgc
11161 tgtagaatgg ggataaaaac atcagagggc acaccagggt ttcgtgcacc tgaagttgcc
11221 agaggaaatg tcatttataa ccaacaggct gatgtttatt catttggttt actactctat
11281 gacattttga caactggagg tagaatagta gagggtttga agtttccaaa tgagtttgat
11341 gaattagaaa tacaaggaaa attacctgat ccagttaaag aatatggttg tgccccatgg
11401 cctatggttg agaaattaat taaacagtgt ttgaaagaaa atcctcaaga aaggcctact
11461 tctgcccagg tctttgacat tttgaattca gctgaattag tctgtctgac gagacgcatt
11521 ttattaccta aaaacgtaat tgttgaatgc atggttgcta cacatcacaa cagcaggaat
11581 gcaagcattt ggctgggctg tgggcacacc gacagaggac agctctcatt tcttgactta
11641 aatactgaag gatacacttc tgaggaagtt gctgatagta gaatattgtg cttagccttg
11701 gtgcatcttc ctgttgaaaa ggaaagctgg attgtgtctg ggacacagtc tggtactctc
11761 ctggtcatca ataccgaaga tgggaaaaag agacataccc tagaaaagat gactgattct
11821 gtcacttgtt tgtattgcaa ttccttttcc aagcaaagca aacaaaaaaa ttttcttttg
11881 gttggaaccg ctgatggcaa gttagcaatt tttgaagata agactgttaa gcttaaagga
11941 gctgctcctt tgaagatact aaatatagga aatgtcagta ctccattgat gtgtttgagt
12001 gaatccacaa attcaacgga aagaaatgta atgtggggag gatgtggcac aaagattttc
12061 tccttttcta atgatttcac cattcagaaa ctcattgaga caagaacaag ccaactgttt
12121 tcttatgcag ctttcagtga ttccaacatc ataacagtgg tggtagacac tgctctctat
12181 attgctaagc aaaatagccc tgttgtggaa gtgtgggata agaaaactga aaaactctgt
12241 ggactaatag actgcgtgca ctttttaagg gaggtaatgg taaaagaaaa caaggaatca
12301 aaacacaaaa tgtcttattc tgggagagtg aaaaccctct gccttcagaa gaacactgct
12361 ctttggatag gaactggagg aggccatatt ttactcctgg atctttcaac tcgtcgactt
12421 atacgtgtaa tttacaactt ttgtaattcg gtcagagtca tgatgacagc acagctagga
12481 agccttaaaa atgtcatgct ggtattgggc tacaaccgga aaaatactga aggtacacaa
12541 aagcagaaag agatacaatc ttgcttgacc gtttgggaca tcaatcttcc acatgaagtg
12601 caaaatttag aaaaacacat tgaagtgaga aaagaattag ctgaaaaaat gagacgaaca
12661 tctgttgagt aagagagaag ggcgaattcg acccagcttt cttgtacaaa gtggtgataa
12721 ttaattaaga taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
12781 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
12841 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
12901 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
12961 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
13021 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
13081 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
13141 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
13201 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
13261 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
13321 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
13381 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
13441 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
13501 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
13561 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
13621 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
13681 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
13741 gagctattc
//
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