如果沒有序列信息，請刪除本句話LOCUS       pHes7-Achilles-Hes7       11926 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses fusion protein of Achilles/YFP and mouse Hes7 from mouse 
            Hes7 promoter. The reporter shows oscillatory expression with 2-3 
            periodicity when expressed in mouse presomitic mesoderm..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11926)
  AUTHORS   Yoshioka-Kobayashi K, Matsumiya M, Niino Y, Isomura A, Kori H, 
            Miyawaki A, Kageyama R
  TITLE     Coupling delay controls synchronized oscillation in the segmentation
            clock.
  JOURNAL   Nature. 2020 Apr;580(7801):119-123. doi: 10.1038/s41586-019-1882-z. 
            Epub 2020 Jan 8.
  PUBMED    31915376
REFERENCE   2  (bases 1 to 11926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            //gt12365.cn
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1882-z"; journalName: "Nature"; date: "2020-04";
            volume: "580"; issue: "7801"; pages: "119-123"
COMMENT     Sequence Label: pHes7-Achilles-Hes7
FEATURES             Location/Qualifiers
     source          1..11926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..17
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6..28
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     25..41
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     25..41
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     60..80
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        62..80
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     117..133
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     117..133
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(167..183)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(168..184)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     regulatory      5568..5577
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5574..5576,5577..5579,5580..6290)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLAVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGSGFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSFLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5619..5640)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5880..5899)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6227..6248
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7070..7081
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9173..9192)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9174..9192)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9202..9219)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9202..9218)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9211..9233)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9360..9815
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9447..9466)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9657..9678
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9841..9945
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9946..10014,10015..10806)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10164..10183)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10977..11565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11466..11485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11719..11736
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11853..11874
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11889..11894,11895..11912,11913..11919)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
       61 caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta
      121 gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc
      181 tcgacgctag gactctaggc atcaccgcat ccagacaccc cattcttcta gagtgaggct
      241 ttgcagaatc acagttctta ccctctgctc cctttctctg tcttttctcc atcataccta
      301 ctgactgcca aataagagtt tatgtaatgc atatggactg tgtccccaca aaagcagaag
      361 ccttttgttc agagctgtct ctgtggggcc tggaacagag caggtcggct aactatgtac
      421 tgtggcaggc agcattcaac ttttgtagct gtggtcttta aagtttgtaa gtgctttcca
      481 gtggagaccc gtcctcaaaa caaacaacaa caaggagaga aaggagggag gaagaggaag
      541 aaagcaataa aaaaagaaaa gaaaaaagtg ttcctttcta ttttatctga gattccccca
      601 gtttagtttt gaatcataga acaaaagacc gagaagttag gccacagtta ctttatgagc
      661 ccagaacgct cccctgcttt gctgttttga ctgagagatt gagtgttctt gctttctaag
      721 gatacagaaa cagctgtcta ggaacatccc cgattcagga cctagggcat cctaaggaga
      781 ccactttccc tttgaagaac taaaggagaa gaagaaggag cttgaaagcc aggcctgggg
      841 agctgccctg caggctaggg gaagttaaaa ggaccaccag ctgctcctag tcttcctttc
      901 accttctgca tccctagagg cctacatgtg gccatgtgct gccctcctcc gaacttcagt
      961 gcggcccacc atagagcaag cgtagggaga catagatgcc tgtatatgcc tgtactgtta
     1021 gtatatagcc atagcatgtc ctctctggac ggaggcttgt gaccttcaga gaaatcctac
     1081 tcataccctc aaacaaaggt accacacaca gccacatctt cacatctgtc agggcaagga
     1141 gctggagtcc aatcacccag ttcattcagc tggtctccct gaagggtaac cttaggctgg
     1201 agggcctgag aagcacatag tgtttatttc tattttttat ttttcaagac agggtttctc
     1261 tttgtaacac tggctgtccc agaactcaga gatgctcctg cctctgcctt ccaagtcctg
     1321 ggattaaaag tgtgtgctgc cactgcctaa tgtacataaa atgtaaaaga aaaaaaaact
     1381 aggcataata agtaatattt agaacaaagc aaagaatgta atgatttcta tcttttaaaa
     1441 attggctctg ggccacttcc gtggtacact ccggaaggta gagtcaggca gctgtctgag
     1501 tttgaggcca gtctggtcta tagggcaagt tccaggacag ccaggagtac acagataaac
     1561 cctgtctcaa aaaataaaaa taaaaataga agttgaccaa tagaggggga aactctgggt
     1621 tcaaccctca atactaatgg aaaataattc tgacaaacta tcagtgaggt aaaacagcaa
     1681 gaaaagcaag accctgtctc agaaatgggt acaacgatga ctgtgatgat aactgatagc
     1741 cagtaagtta tcatcacact gacatgaaat gccagaaaag caacctaact cctggtagac
     1801 ctccacaata ttctcagcag actgcctacc tactttgtat atttttcttg gtgtgtatgt
     1861 gcaatttaca gagttctata ggccctatga acaccttgtt ggaactttcc ccagactgtt
     1921 ttcgggggtc cctcatttgc attagcatca tggtgaatca gccttgcact tgaagttctt
     1981 ctgctccccc caccccctgc gtcccaccgc cccttaaaag ctttcagcct actgggaaag
     2041 cctattttct taaaccagta ggtttgtttt ttcacttttc aaaatgagcc tttctcatca
     2101 aatggcctgc ctatagcact tctgaggccc gtggggataa ccaggagctg gggtggggct
     2161 ggagatgggt gagttgatgt ctgatggggg tggggaatca gcttcagtag cagggaagac
     2221 cccttttcca tcgagtggtc gggagatgga gggtatgatg ttgggaaaag tggggttctc
     2281 taactgctac tctttcttac agaacaatgg ctcaaactag gatgccaact tgaaagtagt
     2341 ctttgttgcc aagccaaggc tggtcttgaa ttctccactg cacttagact aacccccaag
     2401 cttggaatag gctatgcctg acattatcta tctatctatc tatctatcta tctatctatc
     2461 tatctatcta tctatcttat atttcatctt acacctgcca tccccaaatt ctctgttccg
     2521 ggttctcttt ctctaccacc agcagatctc ttttctatag gatttgttcg ttttgcttgg
     2581 actcatggct gaccccgggg agatattatc cccaccgtga agttgcctct gactacttcg
     2641 accgaactag tacttcactg tcacgtcttt gcacttatcc ttacaaccat gtgaaattat
     2701 cctattctgc cttcttggat gtttccggtc ttcacaccac catccccgtc aaaatggacg
     2761 tttcaggggc gctctgagcc ggtgtaaccc gaaagcctaa caagagtggt cacaaagcag
     2821 gcacacacag atttaggctg agtgggcaaa tgtaccagtt gggctcacag agatgccaag
     2881 atgcggtttc cttttctcct agggccatgg cccctgcttg gaaccgagaa gggagagttg
     2941 ttactgacac attccgagac caggctgaaa cgtttaggag acagcccaga gaggagaaga
     3001 aggcttaaac cggaattgag actgtgagag cctgagaagg cagggaggag gggcacagaa
     3061 ggtggcggag cgctccggag gggagcggaa agccagcgcg gggcgtcagg gcccccgggt
     3121 cgctcacaag tggccccagc aggtgaccat gccgggtccc gcctttcccg ggcgcggcgc
     3181 gtgcgggatc aaagctgccg gcaaggggcg ggccagggag ctgcagggaa ctgggggtgc
     3241 gagcgtggga gcgcagggtt gccggaggag acagggacca ggaaagacga cgaggtagcc
     3301 cggagaggcc cagagaggga gcagcagaag gatgtgagac tcagaagccg gaaagtacac
     3361 tctagacccg gagagacccc agcagaagtc tccgagagac ctgaaaaaac tatcagagag
     3421 atggagtcgc tggaagtgct cagggaccag caaaaacgga aaactggaat gcccaggact
     3481 gaatatgggg aaaaggtgct tcagagaaca gaaaatgaaa gggagagaga ggaagaggga
     3541 aagacctgga agacctggcc ctcccctgct gaggaaggag agaactcttg ggcagtgatc
     3601 ccagctccgt gtcctttctc agggttgcgc ctcagggagt gtggacaaag atgtgtgtcg
     3661 aggcagccag aactcccgag ggttaggggt agattgtgag gacctgcgga gtcagagaat
     3721 aattttggaa cgtccaagcc ggccacagaa acaaaacact acatatcagt tttggttaaa
     3781 atgttaaagc gcaagaataa tacttgcttg atgtgctgtc aatttaactt tttcctagtt
     3841 gaggtagacg gaaaaaaaaa aaaagaaaaa agctagaact gtgcattggc cgggaatcga
     3901 acccgggcct cccgcgtggc aggcgagaat tctaccactg aaccaccaat gcagataagg
     3961 agggaggtca taatgagcct atgtgaactt ctcagaggca gatccaatcc tacttctagg
     4021 tgtgggaaaa ggttgtagag aatcattgag gtgggtggag cgtcaggcca tttttttctc
     4081 tccgttggat ccggccaccg ggccataaag tcattccata tggccagggg cggccccaca
     4141 cccgggtgca aactgcctca ggccccgagc ctcacgtgca ggtgagaaaa actcaacccc
     4201 aatcctggtt cccaaggagg ggggcggtgg ggcctgctgg ggccacacat ctgtgcttcc
     4261 atttggctga agtaggggaa ggtgggatgt actgagggcc ctaaccccaa atgggtgcct
     4321 cctttctcaa gtaccgggcc cctcctgctc tgcctttggc aaggcagcct cttgccagaa
     4381 aaccctctca ggatgtggag ggcctctggg aaggggttcc actttgtctc tgactccaca
     4441 gctatcttct ggctcggttt gtagccctgt ctttattatg tcctttcccc aggccttctt
     4501 gtcgtgttaa atttcgattt taccttctca ggcacttcgc cctgaagtct ccaggtgttg
     4561 acatatccat ccttctgaga ggtcatgctc tttgtttgag gtgggtgaag atcccatttt
     4621 gcaaccaaac ctttcttgtc cctttagcaa atctcacgtc tccctgctcc cctacagtcc
     4681 ctagaggcct ccagagcgcc caccaactgg tgcactctat gttttcctgc cagctgtact
     4741 cctgaattta gttttaaacc aagttcttgt aaacggaaag gtatcgcttc tcggcctctt
     4801 ggctaactaa gatcaagggt agtgaatgga aagatttctt cactcacgta tacttcaagg
     4861 attttttttc cccctagaag tttcctttgt cctcccttag tccagccatc cttaggaaaa
     4921 tctgttcccg gggactcaag tcacagtatc tatagagtca gtttggcggc agattctcct
     4981 tattactacg tctgcaggga taagtactgg agttattgtg ggaattgagt agaagagaca
     5041 gatacaacgg cgccgtcttt tcccacagcc agccagtaga atccggtaga tctgctctgc
     5101 acctttaggg gttgggagaa gattataata ttcagcctgg aggcagaagc atgccaacat
     5161 ttatttcagg gttggtgggg ctatagtgaa cctccaagaa atctactagt ctaggagacc
     5221 cccaaatttc atgcctaagt gatttcttcc cagttcactt agactctctt cccctgataa
     5281 tctcctccct ccagttgggt cccgccttat tatacagctt tatggtcctt aaaccagcct
     5341 gtggcccctt cctcccctta cttcccacat ttggggctag ttcctcccct tcccctcccc
     5401 cttcactccc tgcctttccg ggagcctcgt gccggggtcc ttgagctggg catctagggg
     5461 ctgaaggggg cggggccggg ccctagccct cctaccctgc agcggcggga tataaggatc
     5521 taggcagcac gcgctgagtc caccgaaggg tccggaggag cactcgagcc accatggtga
     5581 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     5641 taaacggcca caagttcagc gtgcgcggcg agggcgaggg cgatgccacc atcggcaagc
     5701 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     5761 ccaccctggg ctacggcctg gccgtcttcg cccgctaccc cgaccacatg aagcagcacg
     5821 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     5881 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     5941 gcatcgagct gaagggctcc ggcttcaagg aggacggcaa catcctgggg cacaagctgg
     6001 agtacaactt caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     6061 aggccaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact
     6121 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     6181 gctaccagtc cttcctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     6241 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag gtcacccggg
     6301 agcgagctga gaatagggac ggccccaagg tgagaggtgg gaagggaggg aaatagggtc
     6361 cctgttcagg ccagaggact taaaggttct aactctgtgg actaggagtg aggatggaga
     6421 cacaagagtg aaaagaccga aagaacctgt aaagtataaa gagggcaggg tcagagccca
     6481 acttgcgcgg gctgcggaag cggaggtcta ggggtttgct ttattgtggg ctaaggcttg
     6541 tggctaggaa agtgccagaa tttgggcatc gtggcctatt ctagggctcg ggaagctggg
     6601 gcaggtggat tatcgcaagt tcgaggccac agagtgcacc cctaccgaaa aaacaaacaa
     6661 acaaacaaaa accaaaccaa aataaaacaa acaaacaaaa ccccacgaag ctatactata
     6721 acatgagata agctagaggc catagctggt ggaggagggt ggaaacaaaa atagaggaaa
     6781 ggctatggac gctgtctgta agcgcctggg aacccacatc tgactcaaag agaagacctt
     6841 tgagtcgttg gttccggagg tgcgaggcca ggtgagagat attaggagaa cgagtgaagg
     6901 gtttgagtga gaagaggagg tgtggtcgca ctgtgtgtct gcctttcacc ttaggtctct
     6961 atcgcgcggc tcactagcct gggggcaaag agtggcccta aagttcttca gtgatacact
     7021 gagagggaga cgacacaaga tagagatata aagagaaaag aagagtgcca tagaggggag
     7081 aagagaggag aaagctgaac acagaaaggg cagggagaag tgggcgagcc acggaaagcg
     7141 acccaaggga ctgggaagta gcagggagtg agagggaaac gaatggacca gacccaagca
     7201 gcctgggatt aggggaaagg ggcgcatggg gggttttccc agcggcagga tcggacgctt
     7261 gcgcggcggc gctgagtctc ggtccggtcg gcccgctctt cctttcccgc tggtcacaga
     7321 tgctgaagcc gttggtggag aagcggcgcc gggaccgcat caaccgcagc ctagaagagc
     7381 tgaggctgct gctcctggag aggaccaggg accaggtcag ttccaccgct ggccccaggc
     7441 ccctaagcat tctgtacctg tgtcccagag ctttctactg gggtggggac ataagctgga
     7501 gtggtgactc tggacccaag cctgctcctt gaaatcccat cccagacccc ctctcgctct
     7561 cagaacttgc tcttctccac cttgctggtc tgtaagtttc cagtctggct cggtaaactg
     7621 agcttccagc ctgcgtcagt ttcccacttg gggttttcta aaattcctta tctacctccc
     7681 acgcctgatc aatgggcttc agttctgcgg tggttttcac catccgggtt gctgtttcgt
     7741 acacctctca tattgggaac tggttccact ccctcaggtt ggacaggctc atctcggggt
     7801 gggacgggaa cttttcttgg ccatctccac attcggtcgc gagtcatggg ggcacccgca
     7861 gctcccgtat ccgcccctcc cttcttcccc tagccacttt ccccctctct ccccacccct
     7921 ttctgtctct gcgcccctcc ccccgtctgt agaacctccg gaacccgaag ctggagaaag
     7981 cggagatact ggagttcgcc gtgggctact tgagggagcg aagccgggtg gagcccccgg
     8041 gtacagcgcg gggggtgggg cagcggaggg aggctgggag ggtgggagcc cggaaggctc
     8101 cggccggggg aggggcgggc tctgaggtgg cctgggactt gggggagcgg ccagctgtgc
     8161 accatggcga ggttaataac acccgcgcgt gtctgtctct gtgtctccct cattgccctc
     8221 ctctgcacgt ccatccggcc tcctgtctct gtctgtgcgc ccgtctgacc tcggtatctc
     8281 tgcgccccgc cccttccctc aaccccgttg ggcccctgcc ctgcccgcct gtcctcccgc
     8341 cggccgcacc aggggttccc cggtccccag gccaggacgc cgaggcgctc gccagctgct
     8401 acttgtccgg cttccgcgag tgcctgcttc gcttggcggc ctttgcgcac gacgccagcc
     8461 cggccgcacg ctcccagctt ttctccgcgc tgcacggcta ccggcgcccc aaaccgcccc
     8521 ggcccgaggc cgtagatcca gggctcccag cgccgcgccc accgctggac cccgcttcac
     8581 caatccttgg ccccgcgctg caccagcgcc ccccagtgca ccagggccct cctagtcccc
     8641 gcctcgcttg gtccccatcc cactgctcct ctcgcgccgg ggattcgggc gcgccggcgc
     8701 ccctcaccgg actgctgccg ccaccgccac cgccttacag acaagacggg gcgcccaagg
     8761 ccccgccact cccaccgccc gcgttttgga gaccttggcc ctgagctttt gggggctggg
     8821 gcggggattg ggggtggtgt agagactcca gtcagagggc aggagaggga tccgggcgag
     8881 gagggcagcg gggcgcgcgg gctcagcgcg cgggtgagat gtggtctata ttagagtatc
     8941 tatataaata tatattttcc catccctttt ccctgtccca cttccctgtg tgtagcattg
     9001 tactctctct accctagtcc agtcccagtc ccttcccgag tccctagtgc atggaataaa
     9061 atcgttatta aatcccggtg tgtcccctag ccaggggcct gcctttatat tggcgccacg
     9121 cctacttgcc cataccgcgg ccgcgtcgag ggggggcccg gtacccaatt cgccctatag
     9181 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     9241 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     9301 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     9361 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     9421 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     9481 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     9541 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     9601 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     9661 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     9721 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     9781 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     9841 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     9901 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     9961 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
    10021 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
    10081 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
    10141 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
    10201 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
    10261 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
    10321 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
    10381 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
    10441 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
    10501 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
    10561 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
    10621 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    10681 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    10741 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    10801 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    10861 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    10921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    11041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    11101 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    11161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    11221 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    11281 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    11341 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    11401 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    11461 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    11521 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    11581 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    11641 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    11701 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    11761 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    11821 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    11881 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaa
//