| 質粒名稱: | pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 207507 |
| 存儲實驗室: | Alexander Marson |
|
載體骨架基本信息 |
|
| 載體名稱: | PMSGV |
| 載體出品公司: | Clontech |
| 完整質粒大小: | 8647bp |
| 載體類型: | Retroviral |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | tNGFR, HA-GD2-28z_CAR |
| 物種來源: | H. sapiens (human) |
| 克隆方法: | 未知 |
|
文獻引用方法 |
|
| 材料和方法部分: | pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral was a gift from Alexander Marson (Addgene plasmid # 207507 ; //n2t.net/addgene:207507 ; RRID:Addgene_207507) |
| 參考文獻部分: | Modular pooled discovery of synthetic knockin sequences to program durable cell therapies. Blaeschke F, Chen YY, Apathy R, Daniel B, Chen AY, Chen PA, Sandor K, Zhang W, Li Z, Mowery CT, Yamamoto TN, Nyberg WA, To A, Yu R, Bueno R, Kim MC, Schmidt R, Goodman DB, Feuchtinger T, Eyquem J, Jimmie Ye C, Carnevale J, Satpathy AT, Shifrut E, Roth TL, Marson A. Cell. 2023 Sep 14;186(19):4216-4234.e33. doi: 10.1016/j.cell.2023.08.013. 10.1016/j.cell.2023.08.013 PubMed 37714135 |
買家導航
pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral載體質粒基本信息
pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral質粒圖譜載體圖譜和 pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral載體序列質粒序列多克隆位點信息
pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral質粒載體簡介
pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral質粒序列載體序列
LOCUS pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral 8647 bp DNA circular SYN 14-APR-2025
DEFINITION This plasmid can be used to generate retrovirus..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8647)
AUTHORS Blaeschke F, Chen YY, Apathy R, Daniel B, Chen AY, Chen PA, Sandor
K, Zhang W, Li Z, Mowery CT, Yamamoto TN, Nyberg WA, To A, Yu R,
Bueno R, Kim MC, Schmidt R, Goodman DB, Feuchtinger T, Eyquem J,
Jimmie Ye C, Carnevale J, Satpathy AT, Shifrut E, Roth TL, Marson A
TITLE Modular pooled discovery of synthetic knockin sequences to program
durable cell therapies.
JOURNAL Cell. 2023 Sep 14;186(19):4216-4234.e33. doi:
10.1016/j.cell.2023.08.013.
PUBMED 37714135
REFERENCE 2 (bases 1 to 8647)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
//gt12365.cn
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.cell.2023.08"; journalName: "Cell"; date: "2023-09-14-
14"; volume: "186"; issue: "19"; pages: "4216-4234.e33"
COMMENT Sequence Label: pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral
FEATURES Location/Qualifiers
source 1..8647
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(join(171..962,963..1031))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 794..813
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1032..1136)
/gene="bla"
/label=AmpR promoter
primer_bind 1204..1222
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(1260..1282)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 1382..1401
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 1595..1617
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 1745..1764
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 1964..2480
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
primer_bind 3320..3342
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 3358..3374
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind 3712..3728
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
regulatory 3781..3790
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 4681..4734
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
LTR 6995..7509
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 7681..7697
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(7705..7711,7712..7729,7730..7735))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7750..7771
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7888..7905)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8059..8647)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8139..8158)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
1141 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
1201 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
1261 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
1321 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
1381 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
1441 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
1501 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
1561 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
1621 gcgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
1681 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
1741 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
1801 ccacgatgcg tccggcgtag aggcgattag tccaatttgt taaagacagg atatcagtgg
1861 tccaggctct agttttgact caacaatatc accagctgaa gcctatagag tacgagccat
1921 agataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
1981 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact
2041 gagaatagag aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac
2101 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
2161 atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
2221 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
2281 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
2341 agtcctccga tagactgcgt cgcccgggta cccgtattcc caataaagcc tcttgctgtt
2401 tgcatccgaa tcgtggactc gctgatcctt gggagggtct cctcagattg attgactgcc
2461 cacctcgggg gtctttcatt tggaggttcc accgagattt ggagacccct gcccagggac
2521 caccgacccc cccgccggga ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct
2581 ttgtgcgtgt ttgtgccggc atctaatgtt tgcgcctgcg tctgtactag ttagctaact
2641 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc ggccgcaacc
2701 ctgggagacg tcccagggac ttcgggggcc gtttttgtgg cccgacctga gtcctaaaat
2761 cccgatcgtt taggactctt tggtgcaccc cccttagagg agggatatgt ggttctggta
2821 ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttgggac
2881 cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct ctgtctgact
2941 gtgtttctgt atttgtctga aaatatgggc ccgggctagc ctgttaccac tcccttaagt
3001 ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc
3061 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
3121 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
3181 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
3241 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct
3301 ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt
3361 tatccagccc tcactccttc tctaggcgcc cccatatggc catatgagat cttatatggg
3421 gcacccccgc cccttgtaaa cttccctgac cctgacatga caagagttac taacagcccc
3481 tctctccaag ctcacttaca ggctctctac ttagtccagc acgaagtctg gagacctctg
3541 gcggcagcct accaagaaca actggaccga ccggtggtac ctcaccctta ccgagtcggc
3601 gacacagtgt gggtccgccg acaccagact aagaacctag aacctcgctg gaaaggacct
3661 tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc agcttggata
3721 cacgccgccc acgtgaaggc tgccgacccc gggggtggac catcctctag ccctcgagcc
3781 gccaccatgg gcgctggtgc aactggacgg gccatggatg gaccacggct tttgctcctg
3841 ttgttgttgg gcgtcagcct tggaggggct aaagaggcct gcccgaccgg tttgtacaca
3901 cactccggcg agtgttgtaa ggcgtgtaat cttggcgaag gggtcgccca accgtgtggc
3961 gccaaccaga cggtttgtga gccttgcttg gatagcgtca cgttttccga cgtcgtttca
4021 gccaccgaac catgcaaacc ttgtacggaa tgcgtgggtc tgcagagcat gagcgcaccg
4081 tgcgtagagg cggacgatgc ggtatgccga tgtgcctacg gctactacca agacgagacg
4141 acagggaggt gtgaggcatg cagagtatgt gaggccgggt ctggactcgt attttcctgt
4201 caagacaaac aaaacacagt atgtgaggag tgcccggatg ggacttattc cgatgaagcg
4261 aatcatgtgg acccgtgctt gccatgtacg gtctgtgagg ataccgaaag acagctccga
4321 gaatgcaccc gctgggctga cgcggaatgc gaagaaatcc caggtcgatg gatcacccgc
4381 tcaacccccc ccgaagggtc agattctacg gcgccctcta ctcaggagcc agaagctcca
4441 cccgaacaag acctgatcgc ttctacggtg gcaggtgtcg ttacaacagt catgggttcc
4501 tcacaacctg tggttacgcg ggggacaact gacaatttga ttccagtata ttgtagcatt
4561 ctggccgcgg tcgtggtagg gcttgtcgcg tatatagcgt tcaaaaggtg gaacagcagg
4621 gctaagagag gttcaggatc tggggggggc tcgggaggtg gaaccggtgc tggaagtggt
4681 gagggcaggg gatctctcct tacttgtggc gacgtggagg agaaccccgg ccccatggaa
4741 ttcggcctga gctggctgtt cctggtggcc atcctgaagg gcgtgcagtg cagcagagac
4801 atcctgctga cccagacccc tctgagcctg cctgtgtctc tgggcgatca ggccagcatc
4861 agctgcagat ccagccagag cctggtgcac cggaacggca acacctacct gcactggtat
4921 ctgcagaagc ccggccagag ccccaagctg ctgatccaca aggtgtccaa ccggttcagc
4981 ggcgtgcccg acagattttc tggcagcggc tccggcaccg acttcaccct gaagatcagc
5041 cgggtggaag ccgaggacct gggcgtgtac ttctgcagcc agtccaccca cgtgcccccc
5101 ctgacatttg gcgccggaac aaagctggaa ctgaaggggg gaggcggatc tggcggcgga
5161 ggaagtggcg gagggggatc tgaagtgaag ctgcagcagt ccggccccag cctggtggaa
5221 cctggcgcct ctgtgatgat ctcctgcaag gccagcggca gctccttcac cggctacaac
5281 atgaactggg tgcgccagaa catcggcaag agcctggaat ggatcggcgc catcgacccc
5341 tactacggcg gcaccagcta caaccagaag ttcaagggca gagccaccct gaccgtggac
5401 aagagcagca gcaccgccta catgcacctg aagtccctga ccagcgagga cagcgccgtg
5461 tactactgcg tgtccggcat gaagtactgg ggccagggca caagcgtgac cgtgtctagc
5521 gccaagacca ccccccctag cgtgtacgga agagtgacag tgtcctctgc cgagcccaag
5581 agctgcgaca agacccacac ctgtccccct tgtcctgccc ctcctgttgc aggcccttcc
5641 gtgttcctgt tccccccaaa gcccaaggac acactgatga tcagcagaac ccccgaagtg
5701 acctgcgtgg tggtggacgt gtcccacgag gacccagaag tgaagttcaa ttggtacgtg
5761 gacggcgtgg aagtgcacaa cgccaagaca aagcccagag aggaacagta ccaaagcacc
5821 taccgggtgg tgtccgtgct gaccgtgctg catcaggatt ggctgaacgg caaagagtac
5881 aagtgcaaag tgtccaacaa ggccctgcct gcccccatcg agaaaaccat cagcaaggcc
5941 aagggccagc cccgcgaacc ccaggtgtac acactgcccc ctagcaggga cgagctgacc
6001 aagaaccagg tgtccctgac atgcctcgtg aagggcttct acccctccga tatcgccgtg
6061 gaatgggaga gcaacggcca gcccgagaac aactacaaga caacccctcc cgtgctggac
6121 agcgacggct cattcttcct gtacagcaag ctgacagtgg ataagtcccg gtggcagcag
6181 ggcaacgtgt tcagctgctc cgtgatgcac gaggccctgc acaaccacta cacccagaaa
6241 agcctgtccc tgagccccgg caagaaggac cccaaagcgg ccgcaattga agttatgtat
6301 cctcctcctt acctagacaa tgagaagagc aatggaacca ttatccatgt gaaagggaaa
6361 cacctttgtc caagtcccct atttcccgga ccttctaagc ccttttgggt gctggtggtg
6421 gttgggggag tcctggcttg ctatagcttg ctagtaacag tggcctttat tattttctgg
6481 gtgaggagta agaggagcag gctcctgcac agtgactaca tgaacatgac tccccgccgc
6541 cccgggccca cccgcaagca ttaccagccc tatgccccac cacgcgactt cgcagcctat
6601 cgctccagag tgaagttcag caggagcgca gacgcccccg cgtaccagca gggccagaac
6661 cagctctata acgagctcaa tctaggacga agagaggagt acgatgtttt ggacaagaga
6721 cgtggccggg accctgagat ggggggaaag ccgagaagga agaaccctca ggaaggcctg
6781 tacaatgaac tgcagaaaga taagatggcg gaggcctaca gtgagattgg gatgaaaggc
6841 gagcgccgga ggggcaaggg gcacgatggc ctttaccagg gtctcagtac agccaccaag
6901 gacacctacg acgcccttca catgcaggcc ctgccccctc gctaaggatc cgataaaata
6961 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg
7021 caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact gagaatagag
7081 aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac aggatatctg
7141 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtccc
7201 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
7261 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
7321 ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga
7381 tagactgcgt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
7441 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
7501 gtctttcatg ggtaacagtt tcttgaagtt ggagaacaac attctgaggg taggagtcga
7561 atattaagta atcctgactc aattagccac tgttttgaat ccacatactc caatactcct
7621 gaaatccatc gatggagttc attatggaca gcgcagaaag agctggggag aattgtgaaa
7681 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
7741 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
7801 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
7861 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
7921 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
7981 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
8041 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
8101 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
8161 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
8221 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
8281 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
8341 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
8401 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
8461 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
8521 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
8581 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
8641 atctcaa
//