LOCUS       pMO746       3206 bp    DNA     circular SYN 14-APR-2025
DEFINITION  upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori 
            from pCR4/TOPO, Pnpt-npt-upp;, KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Parks JM, Johs A, Podar M, Bridou R, Hurt RA Jr, Smith SD, Tomanicek
            SJ, Qian Y, Brown SD, Brandt CC, Palumbo AV, Smith JC, Wall JD, 
            Elias DA, Liang L
  TITLE     The genetic basis for bacterial mercury methylation.
  JOURNAL   Science. 2013 Mar 15;339(6125):1332-5. doi: 10.1126/science.1230667.
            Epub 2013 Feb 7.
  PUBMED    23393089
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            //gt12365.cn
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1230667"; journalName: "Science"; date: 
            "2013-03-15- 15"; volume: "339"; issue: "6125"; pages: "1332-5"
COMMENT     Sequence Label: pMO746
FEATURES             Location/Qualifiers
     source          1..3206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(2..793,794..862))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     625..644
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      986..1574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1475..1494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2274..3068)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2385..2404)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2995..3014
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
       61 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
      121 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
      181 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
      241 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
      301 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
      361 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
      421 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
      481 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
      541 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
      601 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
      661 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
      721 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
      781 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
      841 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
      901 gttattgtct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
      961 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1021 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1081 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1141 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1201 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1261 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1321 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1381 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1441 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1501 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1561 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1621 cttttgctca catcttactt ggtgccgaat atcttgtcgc ccgcgtcgcc gagaccgggc
     1681 aggatgtagc cgtgttcgtt caggcgttca tccacggcgg cgacgtagat gtccacatcg
     1741 gggtgcttgt ccacgatgcg cttcagccct tcgggcgcgg cgacgaggaa aagtcccttt
     1801 atctgcgggc agcctgcatt cttgagaagg tcgatggtgg cgtcgagggt gccgccggtc
     1861 gccagcatgg ggtcgaggat gacagccatg cgctcgtcga tgttcttggc gagcttggtg
     1921 tagtactgca ccggttcgag ggtctcctcg ttgcggaaca tgcccaccac gctgaccttg
     1981 gcaccgggaa tcatatccag gaagccgtcg agcatgccga gaccggcgcg caggatgggc
     2041 acggcggtga tcttcttgcc ctttatctgg tcgacctcga caggcccggc ccaaccctgt
     2101 acgctgacct tctccgtttc gaggtccttc gtggcctcgt aggcgagaag acggcagagt
     2161 tcgttggcga tggcccggaa ttcgctcacc gggacatcat gctggcgcag gcggcccagc
     2221 ttgtgtttga ccagcggatg gtcgacgacg taaacagcca cggttgtctc ctttcagaag
     2281 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     2341 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     2401 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa
     2461 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     2521 tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     2581 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     2641 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     2701 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     2761 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     2821 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     2881 tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     2941 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     3001 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     3061 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc cctgcgccat
     3121 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca
     3181 gagggcgccc cagctggcaa ttccgg
//