| 質粒名稱: | pcDNA3.1(+)_Strep-Lrrk2-Myc |
|---|---|
| 出品公司: | Addgene |
| 目錄編號: | 161582 |
| 存儲實驗室: | Maik Hintze |
|
載體骨架基本信息 |
|
| 載體名稱: | pcDNA3.1(+) |
| 載體出品公司: | Invitrogen |
| 空載體大小: | 5322bp |
| 完整質粒大小: | 13004bp |
| 載體類型: | 哺乳細胞表達 |
| 篩選標記: | 新霉素（G418） |
| 載體抗性: | 氨芐霉素 |
| 生長溫度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷貝數: | 高拷貝 |
|
插入基因信息 |
|
| 插入基因名稱: | Lrrk2 |
| 物種來源: | M. musculus (mouse) |
| 插入大小: | 7700bp |
| 基因突變: | 無 |
| 啟動子: | CMV |
| 標簽/融合蛋白: |
StrepII (N terminal on insert)
Myc tag (C terminal on insert)
|
| 克隆方法: | 限制性內切酶 |
| 5’克隆位點: | NheI (unknown if destroyed) |
| 3’克隆位點: | ApaI (unknown if destroyed) |
| 5’測序引物 | GGC TAA CTA GAG AAC CCA CTG |
| 3’測序引物 |
GGC AAC TAG AAG GCA CAG TC
(Common Sequencing Primers)
|
|
文獻引用方法 |
|
| 材料和方法部分: | pcDNA3.1(+)_Strep-Lrrk2-Myc was a gift from Maik Hintze (Addgene plasmid # 161582 ; //n2t.net/addgene:161582 ; RRID:Addgene_161582) |
買家導航
pcDNA3.1(+)_Strep-Lrrk2-Myc載體質粒基本信息
pcDNA3.1(+)_Strep-Lrrk2-Myc質粒圖譜載體圖譜和pcDNA3.1(+)_Strep-Lrrk2-Myc載體序列質粒序列多克隆位點信息
_Strep-Lrrk2-Myc質粒圖譜.png)
pcDNA3.1(+)_Strep-Lrrk2-Myc質粒載體簡介
pcDNA3.1(+)_Strep-Lrrk2-Myc質粒序列載體序列
LOCUS pcDNA3.1(+)_Strep-Lrrk2-Myc 13004 bp DNA circular SYN 14-APR-2025 DEFINITION expression of full-length mouse Lrrk2 with C-terminal Myc tag. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13004) TITLE Lrrk2 expression plasmids REFERENCE 2 (bases 1 to 13004) AUTHORS . TITLE Direct Submission JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1 //gt12365.cn COMMENT SGRef: number: 1; type: "Journal Article" COMMENT Sequence Label: pcDNA3.1(+)_Strep-Lrrk2-Myc FEATURES Location/Qualifiers source 1..13004 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 629..648 /label=T7 /note="T7 promoter, forward primer" promoter 629..647 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 665..674 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" CDS 680..703 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" RBS 1842..1850 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" CDS 8303..8332 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" primer_bind complement(8361..8378) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8367..8591 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8637..9065 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8724..8743) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8934..8955 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(9074..9094) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 9079..9408 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9259..9394 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9321..9340 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 9475..10269 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(9529..9548) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 10139..10158 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 10443..10564 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10480..10499) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10534..10553 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(10613..10629) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10613..10629) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10626..10648) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10637..10653 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(join(10661..10667,10668..10685,10686..10691)) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10706..10727 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10844..10861) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(11015..11603) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(11095..11114) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(join(11774..12565,12566..12634)) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 12397..12416 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12635..12739) /gene="bla" /label=AmpR promoter primer_bind complement(12814..12833) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg 661 gctagccacc atggcaagct ggagccaccc gcagttcgaa aaagccggca tggccagtgg 721 cgcctgtcag ggctgcgaag aggaagagga ggaggaggct ctgaagaagt tgatagtcag 781 gctgaataat gtccaggaag gcaagcagat cgagacgttg cttcagctcc tggaggacat 841 gctggtgttc acctactcgg accgcgcctc caagttattt gaagataaaa atttccacgt 901 gcctctgttg attgtcctgg actcctacat gagagttgcc agtgtacagc aggcggggtg 961 gtcacttctg tgcaaattaa tagaagtctg tccagggaca ttgcaaagct taataggacc 1021 ccaggatatt ggaaatgatt gggaagtcct tggtattcac cggctgattc ttaaaatgtt 1081 aactgttcat cacgccaatg taaacctgtc aatagttgga ctaaaagcct tggatctcct 1141 cctagattca ggtaaactca ccttgctgat actggatgaa gaatgtgata ttttcttgtt 1201 aatttttgat gccatgcaca gatattcagc caatgatgaa gtccaaaaac tgggatgcaa 1261 agctttacac gtgctttttg agagagtttc cgaggaacag ctgactgagt ttgtggagaa 1321 caaagattac acgatactgc tgagtacgtt cggcagcttc agaagggaca aggagattgt 1381 gtaccacgta ctttgctgct tgcattccct ggcggttaca tgcagcaatg tagaggtcct 1441 catgagtggg aatgtccggt gctacaatct tgtggtggag gccatgaaag ccttccccac 1501 caatgaaaac atccaagagg tgagctgctc cttgttccag aagcttacat taggtaactt 1561 tttcaacatc ctggtgttga acgaagtgca tgtctttgtg gtgaaagcgg tccgacagta 1621 tcctgagaac gcagccttac agatctctgc actcagctgt ttagcactcc tcactgagac 1681 tattttctta aaccaagact tggaggaaag aagtgagact caagagcaga gcgaagagga 1741 agacagtgag aagcttttct ggctggaacc ctgctataaa gccctggtgc gccatcgaaa 1801 ggacaaacac gtgcaggagg ctgcctgctg ggcactaaat aacctcctta tgtaccagaa 1861 cagtttgcat gagaagatcg gagatgaaga tggccagttc cctgcgcaca gggaagtgat 1921 gctgtctatg ctgatgcact cttcttccaa agatgtcttc caagcagctg cacatgctct 1981 gtccactctc ttggaacaaa atgttaattt caggaaaatc ctgctggcaa aaggagtata 2041 cctgaatgtc ttggaattga tgcagaagca tgcccatgcg cctgaggtgg cagagagtgg 2101 ctgcaagatg ctgagtcacc tgtttgaagg aagtaaccct tctctggata caatggcagc 2161 agtggtccct aaaatactaa cagtgatgaa agcccacgga acgtctctgt cagtccagct 2221 ggaggcgctg cgagctatct tgcatttcgt tgtgccagga ctattggaag aatccaggga 2281 ggactctcaa tgcagaccaa atgtgctcag aaaacagtgt ttcaggactg acatccacaa 2341 gctggttcta gtcgctctga acaggttcat tgggaatcct gggattcaga aatgtggatt 2401 gaaagtaatc tcttctctcg cgcaccttcc tgatgccaca gagacattgt ccctgcaagg 2461 agcagttgac tcagtcctcc acaccttaca gatgtatcca gatgaccaag aaattcagtg 2521 tctgggctta caccttatgg gatgcttgat gacaaagaag aatttctgca tagggacagg 2581 gcacctcctg gcaaaaattc tggcttccac tttgcagcgc tttaaagatg ttgctgaggt 2641 gcagactaca ggattacaga caaccctgtc aatacttgag ctgtcagtat ctttctccaa 2701 gctgctagtg cactattcct ttgatgtggt gatatttcat cagatgtctt ccagtgttgt 2761 agaacaaaag gatgagcagt tcctcaatct atgttgcaaa tgctttgcaa aagtggccgt 2821 ggatgatgag ctgaaaaaca ccatgctaga gagagcctgc gatcagaata acagcatcat 2881 ggttgaatgt ttgctcctct tgggagctga tgccaaccaa gtgaaggggg caacttcttt 2941 aatctatcag gtatgtgaga aagagagcag tcctaaattg gtggaactgt tgcttaatgg 3001 tggttgtcgt gaacaagatg tacggaaggc cctgaccgta agcatccaaa agggcgacag 3061 ccaggtcatc agcttgctcc tcaggaaact tgccctggac ctggccaaca acagcatttg 3121 ccttggagga tttggcatag gaaaaattga tccttcttgg cttggtcctt tatttccaga 3181 taagtcatcc aatttaagga agcaaacaaa cacaggatct gtcctagcga ggaaagtgct 3241 ccggtatcag atgagaaaca cccttcaaga aggcgtggcc tcaggcagtg acggcaagtt 3301 ttctgaagac gcgctggcga aatttggaga atggaccttt attcccgact cttctatgga 3361 cagtgtgttt ggccagagcg atgatctgga tagcgaaggc agcgagagct catttctcgt 3421 gaagaggaag tccaactcaa ttagtgtagg ggaagtttac agagatctag ctctgcagcg 3481 ctgctcacca aatgctcaga ggcattccaa ttcgctgggt cctgtttttg accatgaaga 3541 cttactgaga cgaaaaagaa aaatactgtc ttcagatgag tctctcaggt cctcaaggct 3601 gccgtcccat atgaggcaat cagatagctc ttcttccctg gcttctgaga gagaacacat 3661 cacgtcgtta gacctatctg ccaacgaact caaagatatt gatgctctga gccagaagtg 3721 ttgcctcagt agccacctgg aacatctcac caaactggaa cttcaccaga attcactcac 3781 gagcttccca cagcagctgt gtgagactct gaagtgtttg atacacttgg atttgcacag 3841 taacaaattc acctcatttc cctctttcgt gttgaaaatg ccacgtatca ccaacctaga 3901 tgcctctcga aatgacatcg ggccaacagt agttttagac cctgcgatga agtgtccaag 3961 cctcaaacag ttgaatctgt cctataacca gctctcttca atcccagaga atcttgccca 4021 agtggtggag aaacttgagc agctcctact ggaaggaaat aaaatatccg ggatttgctc 4081 tcccctgagc ctgaaggaac tgaagatttt aaatcttagt aaaaatcaca ttccatccct 4141 acctggagat tttcttgagg cttgttcaaa agtcgagagt ttcagtgctc gcatgaattt 4201 tcttgctgca atgcctgcct taccttcttc cataacgagc ttaaaattgt ctcagaactc 4261 tttcacgtgc attccagaag cgattttcag tcttccgcac ttgcggtcct tggatatgag 4321 ccacaacaac attgaatgtc tgccgggacc tgcacattgg aagtctctga acttaaggga 4381 actcattttt agcaagaatc agatcagcac cttagacttt agtgagaacc cacacgtgtg 4441 gtcaagagta gagaaactgc atctctctca taataaactg aaagagattc ctccagaaat 4501 tggctgcctt gaaaatctga cgtctctcga cgtcagttac aacttggaac tgaggtcctt 4561 cccaaatgaa atggggaagt tgagcaagat atgggatctt cccttggacg gactgcatct 4621 gaattttgac tttaagcacg taggatgcaa ggccaaagac atcataaggt ttctacaaca 4681 acgtctgaaa aaggctgtac cctacaaccg aatgaagctc atgattgtgg gaaatacggg 4741 gagcggtaag accactttac tgcaacaact catgaaaatg aagaaaccag aacttggcat 4801 gcagggtgcc acagtcggca tagacgtgcg agactggtcc atccaaatac ggggcaaaag 4861 gagaaaggac ctggttctaa acgtgtggga ttttgcaggt cgtgaggaat tctacagcac 4921 tcacccccac ttcatgaccc agagagccct ctacctggct gtctatgatc tcagcaaggg 4981 gcaggcagag gtggacgcca tgaagccctg gctcttcaat atcaaggctc gtgcctcttc 5041 ttccccggtg attctggtgg gcacacattt ggatgtttct gatgagaagc agcggaaagc 5101 gtgcataagc aaaatcacga aggaactcct aaataagcga ggattcccca ccatccggga 5161 ctaccacttt gtgaatgcca ccgaggagtc agatgcgctg gcaaagcttc ggaaaaccat 5221 cataaatgag agccttaatt tcaagatccg agatcagcct gtggttgggc agctaattcc 5281 agattgctac gtagaactgg agaaaatcat tttatcagag cggaaagctg tgccgactga 5341 gtttcctgtg attaaccgga aacacctgtt acagctcgtg aacgaacatc agctgcagct 5401 ggatgagaac gagctcccac acgccgttca cttcctaaat gagtcgggag ttcttctgca 5461 ttttcaagac cctgccctgc agctaagtga cctgtacttt gtggaaccca agtggctttg 5521 taaagtcatg gcacagatct tgacagtgaa ggtagacggc tgtctgaaac atcctaaggg 5581 catcatttcc cggagagatg tggaaaaatt cctttcaaag aagaagcgat tcccgaagaa 5641 ctatatgatg caatacttta aactattaga aaaatttcag atcgcattgc caatagggga 5701 agaatatctt ctggttccaa gcagcttgtc tgaccacagg ccagtgatag agctccccca 5761 ctgtgagaac tctgagatca tcatccggct gtacgaaatg ccgtactttc ccatgggatt 5821 ttggtcaaga ttgattaacc gattacttga aatctcaccc ttcatgcttt ctggcagaga 5881 gagagcacta cgccctaaca ggatgtattg gcggcaaggc atctacttga attggtctcc 5941 agaagcatac tgtctggtag gctctgaagt cttagacaat cgacctgaga gtttcttgaa 6001 aatcacagtt ccgtcttgta gaaaaggttg tattcttctg ggccgagttg tggatcatat 6061 tgactcactc atggaagaat ggtttcccgg gttactggag attgacattt gtggggaagg 6121 agaaactctg ttgaagaaat gggcattgta cagttttaat gatggtgaag aacatcagaa 6181 gatcttgctt gatgagttga tgaagaaggc tgaagaagga gacctgttaa taaatccaga 6241 ccaaccaagg ctcactattc caatatccca gattgctccg gacttgatct tggctgacct 6301 gcctagaaat atcatgttga acaatgatga gttggaattt gaggaagcac cagagtttct 6361 cttaggcgat ggaagttttg gatccgttta tcgagctgcc tacgaaggag aggaagtggc 6421 tgtgaagatt tttaataagc acacatctct taggctgtta agacaagagt tggtggtcct 6481 ttgtcacctt caccacccca gcctgatatc cttgctggcg gctggtattc gtcctcggat 6541 gttggtaatg gagttggcct ccaaaggttc cttggatcgc ctgctgcagc aggacaaagc 6601 cagcctcacc agaaccctcc agcacaggat cgcgttgcat gtggccgacg gcctgaggta 6661 tctccactca gccatgatta tttaccgtga cctgaagccc cacaatgtgc tgctttttac 6721 cctgtatccc aatgctgcca tcattgcgaa gattgcggac tacgggatcg cacagtactg 6781 ctgcaggatg ggaataaaga catcagaggg caccccaggg ttccgggcac ctgaagttgc 6841 cagggggaat gtcatttata accaacaggc cgatgtttat tcttttggct tactacttca 6901 cgatatttgg acaactggga gtaggattat ggagggtttg aggttcccaa atgagtttga 6961 tgagttagcc atacaaggga agttgccaga tccagttaaa gaatatggct gtgccccatg 7021 gcctatggtt gagaagttaa ttacaaagtg tttgaaagaa aatcctcaag aaagacccac 7081 ttctgcccag gtctttgaca ttttgaattc ggctgaatta atttgcctca tgcgacacat 7141 tttaatacct aagaacatca ttgttgaatg catggttgcc acgaatctca atagcaagag 7201 tgcgactctc tggttgggat gtgggaacac agaaaaagga cagctttcct tatttgactt 7261 aaacacggaa agatacagct atgaggaagt tgctgatagt agaatactgt gcttggcttt 7321 ggtgcatctc gctgctgaga aagagagctg ggttgtgtgc gggacacagt ctggggctct 7381 cctggtcatc aatgttgaag aggagacaaa gagacacacc ctggaaaaga tgactgattc 7441 tgtcacttgt ttgcattgca attcccttgc caagcagagc aagcaaagta actttctttt 7501 ggtgggaact gctgatggta acttaatgat atttgaagat aaagccgtta agtgtaaagg 7561 agctgccccc ttgaagacac tacacatagg cgatgtcagt acgcccctga tgtgcctgag 7621 cgagtccctg aattcatctg aaagacacat cacatgggga gggtgtggca caaaggtctt 7681 ctccttttcc aatgatttca ccattcagaa actcatcgag acaaaaacca accagctgtt 7741 ttcttacgca gctttcagcg attctaacat catagcgctg gcagtagaca cagccctgta 7801 tattgccaag aaaaacagcc ctgtcgtaga ggtgtgggac aagaaaacag aaaagctctg 7861 tgaattaata gactgtgtgc acttcttaaa ggaggtgatg gtaaaactaa acaaggaatc 7921 gaaacatcag ctgtcctact ctgggagggt gaaggccctc tgcctgcaga agaacacggc 7981 tctctggatc ggaactggag gaggccacat cttactcctg gatctttcta ctcggcgagt 8041 tatccgcacc attcacaatt tctgtgattc tgtgagagcc atggccacag cacaattagg 8101 aagccttaag aatgtcatgc tggttttggg gtacaagcgg aagagtacag agggtatcca 8161 agaacaaaaa gagatacaat cttgtttgtc tatttgggac ctcaatcttc cacacgaggt 8221 gcaaaattta gaaaaacaca ttgaagtaag aacagaatta gctgataaaa tgaggaaaac 8281 atctgttgaa ggtaccgccc tcgagcaaaa actcatctca gaagaggatc tgtaggggcc 8341 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg 8401 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata 8461 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt 8521 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt 8581 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc 8641 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 8701 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 8761 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 8821 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 8881 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 8941 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 9001 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 9061 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 9121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 9181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 9241 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 9301 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta 9361 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga 9421 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt 9481 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 9541 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 9601 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac 9661 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 9721 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 9781 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 9841 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 9901 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 9961 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag 10021 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 10081 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 10141 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 10201 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 10261 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat 10321 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 10381 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 10441 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 10501 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 10561 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 10621 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 10681 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 10741 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 10801 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 10861 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 10921 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 10981 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 11041 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 11101 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 11161 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 11221 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 11281 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 11341 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 11401 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 11461 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 11521 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 11581 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 11641 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 11701 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 11761 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 11821 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 11881 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 11941 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 12001 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 12061 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 12121 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 12181 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 12241 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 12301 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 12361 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 12421 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 12481 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 12541 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 12601 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 12661 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 12721 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg 12781 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 12841 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat 12901 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag 12961 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtt //
pcDNA3.1(+)_Strep-Lrrk2-Myc載體圖譜質粒圖譜pdf版和pcDNA3.1(+)_Strep-Lrrk2-Myc載體序列質粒序列等相關資料下載
pcDNA3.1(+)_Strep-Lrrk2-Myc質粒載體應用舉例
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